• Journal of Wetlands Research
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• v.14 no.1
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• pp.59-73
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• 2012

The importance of rice paddy wetland has been raised since the 10th Ramsar Convention in 2010. However, there is shortage of study on the evaluation of rice paddy wetland and also of the study on the effect of organic agriculture on the vegetation characteristics. Accordingly, this study examined the vegetation characteristics of organic rice paddy for the basic resources of evaluating value of rice paddy wetland. 6 places of organic rice paddy and conventional rice paddy were chosen as research targets. It analyzed the function of 'Floral Diversity and Wildlife Habitat' among the revised RAM, an existing wetland evaluation system. As to the factor affecting the analysis result, simple land-use result was proved to determine the evaluation. As a result of vegetation investigation total 176 taxa, 53 families, 146 generics, 148 species, 26 varieties and 1 forma. When the difference of appearance of life form between organic area and conventional area was examined, organic paddy had higher appearance of life form in Therophyte and Megaphanerophyte. For the distribution of Naturalized plants, organic rice paddy had lower naturalized rate and urbanization index than conventional rice paddy. As to the Pearson correlation analysis between growing condition and vegetation characteristics, variety of rice paddy vegetation showed it was not heavily influenced by the land use. However, the organic rice paddy area had more variety in vegetation than conventional rice paddy. There was about 1% correlation with types of Cyperaceae, which means that the classification group of Cyperaceae can be utilized in evaluating rice paddy wetland later on. It is determined that the wetland evaluation has been widely influenced by soil environment, water environment and surrounding natural and artificial landscape as well as vegetation characteristics. Accordingly, further research seems to be required with minute investigation to an extensive area.

• Journal of the Korean Association of Geographic Information Studies
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• v.18 no.4
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• pp.81-99
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• 2015

The acceleration of global warming has required better understanding of carbon cycles over local and regional areas such as the Korean peninsula. Since forests serve as a carbon sink, which stores a large amount of terrestrial carbon, there has been a demand to accurately estimate such forest carbon sequestration. In Korea, the National Forest Inventory(NFI) has been used to estimate the forest carbon stocks based on the amount of growing stocks per hectare measured at sampled location. However, as such data are based on point(i.e., plot) measurements, it is difficult to identify spatial distribution of forest carbon stocks. This study focuses on urban areas, which have limited number of NFI samples and have shown rapid land cover change, to estimate grid-based forest carbon stocks based on UNFCCC Approach 3 and Tier 3. Land cover change and forest carbon stocks were estimated using Landsat 5 TM data acquired in 1991, 1992, 2010, and 2011, high resolution airborne images, and the 3rd, 5th~6th NFI data. Machine learning techniques(i.e., random forest and support vector machines/regression) were used for land cover change classification and forest carbon stock estimation. Forest carbon stocks were estimated using reflectance, band ratios, vegetation indices, and topographical indices. Results showed that 33.23tonC/ha of carbon was sequestrated on the unchanged forest areas between 1991 and 2010, while 36.83 tonC/ha of carbon was sequestrated on the areas changed from other land-use types to forests. A total of 7.35 tonC/ha of carbon was released on the areas changed from forests to other land-use types. This study was a good chance to understand the quantitative forest carbon stock change according to the land cover change. Moreover the result of this study can contribute to the effective forest management.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.27-28
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• 2014

Beauveria bassiana (Cordycipitaceae, Hypocreales, Ascomycota) is an anamorphic fungus having a potential to be used as a biological control agent because it parasitizes a wide range of arthropod hosts including termites, aphids, beetles and many other insects. A number of bioactive secondary metabolites (SMs) have been isolated from B. bassiana and functionally verified. Among them, beauvericin and bassianolide are cyclic depsipeptides with antibiotic and insecticidal effects belonging to the enniatin family. Non-ribosomal peptide synthetases (NRPSs) play a crucial role in the synthesis of these secondary metabolites. NRPSs are modularly organized multienzyme complexes in which each module is responsible for the elongation of proteinogenic and non-protein amino acids, as well as carboxyl and hydroxyacids. A minimum of three domains are necessary for one NRPS elongation module: an adenylation (A) domain for substrate recognition and activation; a tholation (T) domain that tethers the growing peptide chain and the incoming aminoacyl unit; and a condensation (C) domain to catalyze peptide bond formation. Some of the optional domains include epimerization (E), heterocyclization (Cy) and oxidation (Ox) domains, which may modify the enzyme-bound precursors or intermediates. In the present study, we analyzed genomes of B. bassiana and its allied species in Hypocreales to verify the distribution of NRPS-encoding genes involving biosynthesis of beauvericin and bassianolide, and to unveil the evolutionary processes of the gene clusters. Initially, we retrieved completely or partially assembled genomic sequences of fungal species belonging to Hypocreales from public databases. SM biosynthesizing genes were predicted from the selected genomes using antiSMASH program. Adenylation (A) domains were extracted from the predicted NRPS, NRPS-like and NRPS-PKS hybrid genes, and used them to construct a phylogenetic tree. Based on the preliminary results of SM biosynthetic gene prediction in B. bassiana, we analyzed the conserved gene orders of beauvericin and bassianolide biosynthetic gene clusters among the hypocrealean fungi. Reciprocal best blast hit (RBH) approach was performed to identify the regions orthologous to the biosynthetic gene cluster in the selected fungal genomes. A clear recombination pattern was recognized in the inferred A-domain tree in which A-domains in the 1st and 2nd modules of beauvericin and bassianolide synthetases were grouped in CYCLO and EAS clades, respectively, suggesting that two modules of each synthetase have evolved independently. In addition, inferred topologies were congruent with the species phylogeny of Cordycipitaceae, indicating that the gene fusion event have occurred before the species divergence. Beauvericin and bassianolide synthetases turned out to possess identical domain organization as C-A-T-C-A-NM-T-T-C. We also predicted precursors of beauvericin and bassianolide synthetases based on the extracted signature residues in A-domain core motifs. The result showed that the A-domains in the 1st module of both synthetases select D-2-hydroxyisovalerate (D-Hiv), while A-domains in the 2nd modules specifically activate L-phenylalanine (Phe) in beauvericin synthetase and leucine (Leu) in bassianolide synthetase. antiSMASH ver. 2.0 predicted 15 genes in the beauvericin biosynthetic gene cluster of the B. bassiana genome dispersed across a total length of approximately 50kb. The beauvericin biosynthetic gene cluster contains beauvericin synthetase as well as kivr gene encoding NADPH-dependent ketoisovalerate reductase which is necessary to convert 2-ketoisovalarate to D-Hiv and a gene encoding a putative Gal4-like transcriptional regulator. Our syntenic comparison showed that species in Cordycipitaceae have almost conserved beauvericin biosynthetic gene cluster although the gene order and direction were sometimes variable. It is intriguing that there is no region orthologous to beauvericin synthetase gene in Cordyceps militaris genome. It is likely that beauvericin synthetase was present in common ancestor of Cordycipitaceae but selective gene loss has occurred in several species including C. militaris. Putative bassianolide biosynthetic gene cluster consisted of 16 genes including bassianolide synthetase, cytochrome P450 monooxygenase, and putative Gal4-like transcriptional regulator genes. Our synteny analysis found that only B. bassiana possessed a bassianolide synthetase gene among the studied fungi. This result is consistent with the groupings in A-domain tree in which bassianolide synthetase gene found in B. bassiana was not grouped with NRPS genes predicted in other species. We hypothesized that bassianolide biosynthesizing cluster genes in B. bassiana are possibly acquired by horizontal gene transfer (HGT) from distantly related fungi. The present study showed that B. bassiana is the only species capable of producing both beauvericin and bassianolide. This property led to B. bassiana infect multiple hosts and to be a potential biological control agent against agricultural pests.

• Korean Journal of Environment and Ecology
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• v.32 no.5
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• pp.445-468
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• 2018

The purpose of this study was to investigate the distribution of vascular plants growing at the Mt. Daedun provincial park area in the boundary between Nonsan-si, Geumsan-gun, Chungcheongnam-do and Wanju-gun, Jeollabuk-do, Korea. We collected the vascular plants 9 times from May 2016 to July 2017. The flora of Mt. Daedun was classified as a total of 434 taxa comprising of 95 families, 278 genera, 388 species, 3 subspecies, 38 varieties and 5 forma. Among them, the endemic plants in Korea that the investigation found were 11 taxa including Broussonetia kazinoki Siebold, Clematis trichotoma Nakai, Thalictrum actaefolium var. brevistylum Nakai, etc. It also found rare plant species, as designated by the Korea Forest Service were 6 taxa including 5 grade LC taxa such as Eranthis stellata Maxim., Viola albida Palib., and Scopolia japonica Maxim. and 1 grade DD taxa, Scrophularia koraiensis Nakai. The special floristic plants were a total of 35 taxa such as 9 taxa in degree III (Eranthis stellata Maxim., Stewartia pseudocamellia Maxim., Acer palmatum Thunb., etc.), 7 taxa in degree II (Equisetum hyemale L., Potentilla dickinsii Franch.&Sav., Viola orientalis (Maxim.) W. Becker, etc.) and 19 taxa in degree I (Dryopteris crassirhizoma Nakai, Cephalotaxus koreana Nakai, Ulmus parvifolia Jacq., etc.) to provide basic data. No taxa in grade V or IV were found. The naturalized plants were 34 taxa including Rumex acetosella L., Phytolacca americana L. and Chenopodium album L., and the percentage of urbanization index was 10.6% while the naturalized plant index was 7.7%.

• Korean Journal of Environment and Ecology
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• v.32 no.5
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• pp.469-477
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• 2018

Hanabusaya asiatica is an endemic species whose distribution is limited in the mid-eastern part of the Korean peninsula. Due to its narrow range and small population, it is necessary to protect its habitats by identifying it as Key Biodiversity Areas (KBAs) adopted by the International Union for Conservation of Nature (IUCN). In this paper, we estimated potential natural habitats for H. asiatica using maximum entropy model (MaxEnt) and identified candidate sites for KBA based on the model results. MaxEnt is a machine learning algorithm that can predict habitats for species of interest unbiasedly with presence-only data. This property is particularly useful for the study area where data collection via a field survey is unavailable. We trained MaxEnt using 38 locations of H. asiatica and 11 environmental variables that measured climate, topography, and vegetation status of the study area which encompassed all locations of the border region between South and North Korea. Results showed that the potential habitats where the occurrence probabilities of H. asiatica exceeded 0.5 were $778km^2$, and the KBA candidate area identified by taking into account existing protected areas was $1,321km^2$. Of 11 environmental variables, elevation, annual average precipitation, average precipitation in growing seasons, and the average temperature in the coldest month had impacts on habitat selection, indicating that H. asiatica prefers cool regions at a relatively high elevation. These results can be used not only for identifying KBAs but also for the reference to a protection plan for H. asiatica in preparation of Korean reunification and climate change.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.1
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• pp.13-18
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• 2009

Accurate solar radiation data are critical to evaluate major physiological responses of plants. For most upland crops and orchard plants growing in complex terrain, however, it is not easy for farmers or agronomists to access solar irradiance data. Here we suggest a simple method using a sun-slope geometry based topographical coefficient to estimate daily solar irradiance on any sloping surfaces from global solar radiation measured at a nearby weather station. An hourly solar irradiance ratio ($W_i$) between sloping and horizontal surface is defined as multiplication of the relative solar intensity($k_i$) and the slope irradiance ratio($r_i$) at an hourly interval. The $k_i$ is the ratio of hourly solar radiation to the 24 hour cumulative radiation on a horizontal surface under clear sky conditions. The $r_i$ is the ratio of clear sky radiation on a given slope to that on a horizontal reference. Daily coefficient for slope correction is simply the sum of $W_i$ on each date. We calculated daily solar irradiance at 8 side slope locations circumventing a cone-shaped parasitic volcano(c.a., 570m diameter for the bottom circle and 90m bottom-to-top height) by multiplying these coefficients to the global solar radiation measured horizontally. Comparison with the measured slope irradiance from April 2007 to March 2008 resulted in the root mean square error(RMSE) of $1.61MJ\;m^{-2}$ for the whole period but the RMSE for April to October(i.e., major cropping season in Korea) was much lower and satisfied the 5% error tolerance for radiation measurement. The RMSE was smallest in October regardless of slope aspect, and the aspect dependent variation of RMSE was greatest in November. Annual variation in RMSE was greatest on north and south facing slopes, followed by southwest, southeast, and northwest slopes in decreasing order. Once the coefficients are prepared, global solar radiation data from nearby stations can be easily converted to the solar irradiance map at landscape scales with the operational reliability in cropping season.

• Journal of the Korean Institute of Landscape Architecture
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• v.37 no.2
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• pp.114-123
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• 2009

To investigate the possible use of plants for landscaping in reclaimed soil, a planting pilot system experiment was performed over the course of four years in reclaimed dredging area with four species: Alnus firma, Alnus hirsuta, Pinus thunbergii, and Pyrachantha angustifolia for 4 years. The physicochemical characteristics of the tested soil showed that it was sandy through coming from a reclaimed dredging area. The average pH of the tested soil was 7.16(slight alkali), and electric conductivity(EC) was relatively low, $294{\mu}S/cm$, even though it came from a saltwater area. To test the effect of planting density vs. phytomass by plant specie from a planting basin, the experiment was designed using four plant species with high and low planting densities over 4 years. The planting conditions of the growth of landscape tree species exhibited growth height as follows: A. hirsuta, A. firma, P. thunbergii, and P. angustifolia, whill the DBH followed the order of A. hirsuta, A. firma, and P. thunbergii. The total phytomass of each plant was higher at low density planting areas than high density planting area in terms of total phytomass production and growth distribution in the reclaimed dredging area. Total phytomass per unit area increased as follows: A. hirsuta, A. firma, P. thunbergii, and P. angustifolia. The total phytomass per each tested plant was 2 times higher in low density planting areas than high density planting areas. Total phytomass per unit area, however, was similar or slighty higher in high density planting areas compared to low density areas. Among the tested plants, A. hirsuta showed the highest phytomass, implying that A. hirsuta adapted very well to the reclaimed area and has the capability of a fast growth, nitrogen fixation tree, and utilizing insoluble nutrients through inoculated root nodule bacteria. The yield of phytomass per individual in low density Alnus species was greater than that of the high density. However, those per unit areas had no difference in the density-dependent planting. The ratio of belowground to aboveground was $0.21{\sim}0.26$. Thus, it could be concluded that the Alnus species are potential candidates for ornamental tree species in reclaimed dredging areas. This study offers baseline data for the use of ornamental tree species in reclaimed dredging areas. Additional research is required for different ornamental species in order to increase phytomass of a planting conditions based on reclaimed dredging areas.

• Korean Journal of Community Nutrition
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• v.1 no.1
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• pp.9-27
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• 1996

The nutritional status is strictly related with flood production, flood processing, and distribution along with habits, education and technological achievement, adapted and adjusted to socio-economic conditions. All these factors are independently affecting the nutritional status of populations. In addition to the above mentioned factors launch of it is useful to consider two points ; unification of the South and the North Korea and WTO. The present study gives and overview of basic knowledge about nutritional status of Korean by using availab1e data in relation to nutrition. The basic characteristics of Korean diet with proportions high in carbohydrate and low in fat, have been relatively constant for the past two decades. The average daily flood intake in terms of weight of flood per person is relatively constant throughout the years. Although the proportion of animal food intake tends to increase recently, the Korean diet is still insufficient in meat, eggs, milk, and fish. Moreover because milk has not been accustomed flood with the general population and not much used in traditional flood preparation in Korea, milk consumption was especially low in comparison with western countries. The total energy intake was relatively constant throughout the years from 1969 to 1993. However, changes in the composition have occurred in the past two decades. The amount and proportions of fat have been s1ightly increasing while the total amount of carbohydrate has been decreasing. The nutrition preblems of Korea have changed over the past severa1 decades. The general adequacy of protein and energy existing after Korean War(1950) was resolved now. Since then the average diet appears to be nearly desirable in terms of rapid rates of growth during childhood and attainment of progressively stature and body weight at maturity. The dietary habits of some young people in these days seem to be taking a more western style diets. This trends if established by habit may lead to a marked change in the traditional diet and health. I think Korean nutritional experiences have potential values for tole other countries in Asia and in western countries. Korean diet illustrates a high level of nutritional status and health attainable will a largely vegetable diet : high in complex carbohydrates, and dietary fibre, and low in tat, and reasonable amount of total protein. This is significant for developing and developed countries that must select specific goals fir adequate nutrition for the people. Compared to the western countries, Koreas different incidence of coronary heart disease and malignancy demonstrates the significance of environment and probably the prominent role of diet in the development of these diseases. The changes occurring in the Korean diet of fir the unusual opportunity to assess the effect of diet upon chronic degenerative disease. In the future, the Korean diet might be continue to change significantly These changes are being influenced by socioeconomic factors that have been emerging and growing stronger since mid-l980 and that probably continue to be potent. The expanded purchasing power of the consumer results in increased discretionary purchases. In the case of foodstuffs, the consumer demands appear to be directed toward items of higher protein content, which, being primarily animal products, are inevitably accompanied by an increased consumption of fat. The continued availability of these more expensive flood items depends upon the balance of foreign trade favouring their importation and domestic production. A regression of foreign trade could result in a decreased supply for the consumer, whereas continued growth of trade iou]d provide freedom for increasing availability to the consumer. In this latter situation the exact choice of foodstuffs is depending upon comsumer tastes and the pressures that may influence it.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.2
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• pp.108-125
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• 2015

The agro-climatic index is one of the ways to assess the climate resources of particular agricultural areas on the prospect of agricultural production; it can be a key indicator of agricultural productivity by providing the basic information required for the implementation of different and various farming techniques and practicalities to estimate the growth and yield of crops from the climate resources such as air temperature, solar radiation, and precipitation. However, the agro-climate index can always be changed since the index is not the absolute. Recently, many studies which consider uncertainty of future climate change have been actively conducted using multi-model ensemble (MME) approach by developing and improving dynamic and statistical downscaling of Global Climate Model (GCM) output. In this study, the agro-climatic index of Korean Peninsula, such as growing degree day based on $5^{\circ}C$, plant period based on $5^{\circ}C$, crop period based on $10^{\circ}C$, and frost free day were calculated for assessment of the spatio-temporal variations and uncertainties of the indices according to climate change; the downscaled historical (1976-2005) and near future (2011-2040) RCP climate sceneries of AR5 were applied to the calculation of the index. The result showed four agro-climatic indices calculated by nine individual GCMs as well as MME agreed with agro-climatic indices which were calculated by the observed data. It was confirmed that MME, as well as each individual GCM emulated well on past climate in the four major Rivers of South Korea (Han, Nakdong, Geum, and Seumjin and Yeoungsan). However, spatial downscaling still needs further improvement since the agro-climatic indices of some individual GCMs showed different variations with the observed indices at the change of spatial distribution of the four Rivers. The four agro-climatic indices of the Korean Peninsula were expected to increase in nine individual GCMs and MME in future climate scenarios. The differences and uncertainties of the agro-climatic indices have not been reduced on the unlimited coupling of multi-model ensembles. Further research is still required although the differences started to improve when combining of three or four individual GCMs in the study. The agro-climatic indices which were derived and evaluated in the study will be the baseline for the assessment of agro-climatic abnormal indices and agro-productivity indices of the next research work.

• Journal of Food Hygiene and Safety
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• v.32 no.3
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• pp.187-192
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• 2017

The consumption of herbal medicines has been increasing with growing interest in health. However, due to recent climate change and the complex distribution process of herbal medicines with high import dependence the likelihood of contamination with mycotoxin has been increased. Mycotoxins are emerging as key indicators for ensuring safety of herbal medicines. A total of 498 herbal medicine samples were screened for mycotoxin contamination in this study. Aflatoxin in the herbal medicine samples was extracted by using immunoaffinity column, then the extracted aflatoxin was quantified via high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) method. The extraction method was verified by linearity, recovery, LOD and LOQ. Aflatoxins were detected in 39/498 samples in an average of $7.670{\mu}g/kg$ ($0.610-77.452{\mu}g/kg$ range). Although safety standards for Corydalis Tuber is not currently available in korea, five of the 39 samples had high concentration of aflatoxins (average of $14.9{\pm}4.1{\mu}g/kg$). In conclusion, it is urgent to establish safety criteria of aflatoxin in Corydalis Tuber. The results of the current study suggest that continuous monitoring is necessary for proactive management of herbal medicine safety.