• Journal of Power Electronics
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• 제9권2호
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• pp.146-155
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• 2009

The static frequency converter (SFC) systems are used as a method of driving large synchronous machines in many power and industrial plants. In this paper, new control algorithms of SFC systems for starting gas turbo sets are proposed for a four quadrant operation: start-up at standstill; an acceleration up to the speed of the rated voltage; field weakening to reach the rated speed; synchronization to the main alternating current (AC) source; and dynamic braking to stop safely within the rating of the synchronous machine. Experimental results show that the proposed algorithms are proper and effective.

• 한국발생생물학회지:발생과생식
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• 제21권1호
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• pp.47-54
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• 2017

Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect of cell cycle inhibitors on the cell cycle synchronization of piPSCs. piPSCs were generated using combination of six human transcriptional factors under stem cell culture condition. To examine the efficiency of cell cycle synchronization, piPSCs were cultured on a matrigel coated plate with stem cell media and they were treated with staurosporine (STA, 20 nM), daidzein (DAI, $100{\mu}M$), roscovitine (ROSC, $10{\mu}M$), or olomoucine (OLO, $200{\mu}M$) for 12 h. Flow Cytometry (FACs) data showed that piPSCs in control were in G1 ($37.5{\pm}0.2%$), S ($34.0{\pm}0.6%$) and G2/M ($28.5{\pm}0.4%$). The proportion of cells at G1 in DAI group was significantly higher than that in control, while STA, ROSC and OLO treatments could not block the cell cycle of piPSCs. Both of viability and apoptosis were affected by STA and ROSC treatment, but there were no significantly differences between control and DAI groups. Real-Time qPCR and FACs results revealed that DAI treatment did not affect the expression of pluripotent gene, Oct4. In case of OLO, it did not affect both of viability and apoptosis, but Oct4 expression was significantly decreased. Our results suggest that DAI could be used for synchronizing piPSCs at G1 stage and has any deleterious effect on survival and pluripotency sustaining of piPSCs.

• 인간행동과 음악연구
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• 제16권1호
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• pp.47-72
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• 2019

본 연구는 자폐스펙트럼장애(ASD) 아동의 운동조절을 평가하기 위해 리드믹 운동과제를 포함한 국외 실험연구를 고찰하고 분석하고자 하였다. 이를 위해 ASD로 진단받은 아동과 신경학적 손상이 없는 대조군(TD)을 대상으로 하고 리드믹 탭핑, 리드믹 운동, 동기화 등 운동조절 관련 변인을 포함하고 있는 국외 실험연구를 선정하였다. 총 10개의 연구가 최종 선정되었고, 해당 연구에 포함된 운동조절 관련 변인과 사용된 청각 자극, 측정 방법 등을 분석하였다. 또한 ASD 아동과 TD 아동의 리드믹 운동과제 수행력 차이를 확인하기 위해 메타분석을 실시하였다. 연구 결과, 리드믹 운동과제를 통해 운동조절을 평가할 때 목표된 변인은 다섯 가지로 운동 타이밍 조절, 타이밍 재산출, 양손 협응, 동기화, 대인 간 동기화가 포함되었고, 각 과제는 움직임의 정확도(accuracy)와 정밀도(precision)의 측면에서 분석됨을 알 수 있었다. 또한 리드믹 운동과제 수행력과 사회기술 지표의 상관관계가 보고됨을 확인할 수 있었다. 메타분석 결과, ASD 그룹은 청각 자극에 맞추어 움직이다가 자극이 소거된 후에도 동일한 움직임을 지속하는 타이밍 재산출 지표에 있어서는 TD 그룹과 유의한 차이가 없는 반면, 외부 자극(청각적 큐나타인)에 동기화하고 움직임을 일관적으로 조절하는 데 있어서는 유의하게 낮은 수행 수준을 보이는 것으로 나타났다. 이러한 연구 결과는 리드믹 운동과제가 음악치료 임상 현장에서 ASD 아동의 운동조절뿐만 아니라 사회기술을 평가하는 효과적인 방안이 될 수 있음을 시사한다.

• 한국콘텐츠학회논문지
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• 제11권11호
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• pp.194-203
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• 2011

불완전 척수손상환자에게 시각적 바이오피드백 시뮬레이션훈련의 적용을 통해 균형에 미치는 영향을 알아보고자 하였다. 불완전 척수손상환자 중 기능향상을 기대할 수 있는 ASIA장애척도 C, D환자 15명을 실험군 7명, 대조군 8명으로 무작위 분류하였다. 균형진단과 바이오피드백 훈련이 가능한 Tetrax Interactive Balance System을 이용하여 시각적 바이오피드백 시뮬레이션훈련을 6주간 일주에 3회씩, 1회에 15분간 총 18회를 실시하였다. 분석방법은 집단 내 전과 후의 차이는 대응표본 t검정을 시행하였고, 두집단 사이 전후 변화량의 차이는 독립표본 t검정을 사용하였다. 실험군은 자세 흔들림을 측정하는 안정성지수와 낙상의 위험정도를 나타내는 낙상지수 및 각각의 체중 변화를 감지하는 체중분포도(A, C, D, LEFT, HEEL)와 체중분포지수에서 유의하게 향상되었고, 자세조절능력을 나타내는 동조화에서는 좌우뒤꿈치(AC)간과 좌우앞꿈치(BD)간을 제외하고 모두 유의하게 향상되었다(p<.05). 대조군은 체중분포도(A, C, LEFT)에서만 유의하게 향상되었다(p<.05). 실험군과 대조군간의 변화량에서 체중분포도(A, B, C, HEEL)와 안정성지수 및 낙상지수, 그리고 동조화 AD에서만 유의한 향상을 보였다(p<.05). 이 연구의 결과로 볼 때 불완전 척수손상환자에게 부가적으로 균형증진을 위한 시각적 바이오피드백 시뮬레이션훈련은 균형능력향상에 있어서 유용할 것이며 추후 더 많은 연구들이 시행되어야 할 것이다.

• Journal of electromagnetic engineering and science
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• 제4권2호
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• pp.72-78
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• 2004

In this paper, we evaluate the performance of the digital modulation schemes described in the APCO Project 25 FDMA specifications which can be used for applying the ultra-narrowband technology to the current domestic simple two-way radio systems, and discuss difficulties in the DSP implementation of the systems. We analyze the effect on the systems' BER performance of receiver non-matched filter and frequency-offset between the transmitter and receiver oscillators. And we present a frequency offset compensation method for improving the system performance. The results of performance analysis showed that the CQPSK of APCO Project 25 using non-matched filter degraded the BER by 0.5～1.0 ㏈ comparing with PI/4 DQPSK using matched filter. In the event of 2 % frequency offset, about 1 ㏈ performance loss was produced at the BER of $$10^{-3}TEX>. With the frequency-offset compensation method implemented in the systems using phase recovery scheme of PSK synchronization detection, the performance degradation of about 1.0 ㏈ was occurred at the BER of $$10^{-3}TEX> for 10 % of frequency offset. The proposed method can be used for the improvement of system performance.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.140-140
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• 2003

The study evaluated the effect of donor cell treatments for G0/Gl synchronization and the donor ceil type on development and incidence of apoptosis in cloned cattle embryos. Primary cultures were established from a female fetus on day 50 of gestation and adult ear skin biopsies. Cells were randomly allocated into 3 experimental treatment groups after 6~8 passages. Group 1 (Confluent), cells were cultured in DMEM supplemented with 10% FBS until 90% confluent. Group 2 (Serum-starvation), cells were cultured in DMEM Supplemented With 0.5% FBS for 5 days. Group 3 (Roscovitine), Cells were cultured in DMEM supplemented with 10% FBS and 30 $\mu$M Roscovitine for 12 h. Cell cycle and apoptosis were analyzed using flow cytometry after labelling with DAPI and YO-PRO-1. At 19 h post-maturation (hpm), enucleated oocytes were reconstructed with donor cells and fused by a single DC pulse (1.6 kV/cm, 60 $\mu$sec). (중략)

• Clinical and Experimental Reproductive Medicine
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• 제48권4호
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• pp.352-361
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• 2021

Objective: The study assessed the developmental potential of germinal vesicle (GV) oocytes subjected to in vitro maturation (IVM) after prematuration culture with cilostamide (a phosphodiesterase-3 inhibitor) and the impact of cilostamide exposure on the morphology of meiosis II (MII) oocytes and subsequent embryo quality. Methods: In total, 994 oocytes were collected from 63 patients. Among 307 GV oocytes, 140 oocytes were selected for the experimental group and 130 oocytes for the control group. The denuded GV-stage oocytes were cultured for 6 hours with cilostamide in the experimental group and without cilostamide in the control group. After 6 hours, the oocytes in the experimental group were washed and transferred to fresh IVM medium. The maturational status of the oocytes in both groups was examined at 26, 36, and 48 hours. Fertilization was assessed at 18 hours post-intracytoplasmic sperm injection. Embryo quality was assessed on days 3 and 5. Results: In total, 92.1% of the oocytes remained in the GV stage, while 6.4% converted to the MI stage (p<0.01) after cilostamide exposure. In both groups, more MII oocytes were observed at 36 hours (25.8% vs. 21.5%) than at 26 hours (10.8% vs. 14.6%) and 48 hours (13% vs. 7.9%) (p>0.05). With the advent of cilostamide, blastocyst quality was better in the experimental group than in the control group (p<0.05). Conclusion: Cilostamide effectively blocked nuclear maturation and promoted cytoplasmic growth. Prematuration culture with cilostamide enabled synchronization between cytoplasmic and nuclear maturity, resulting in better blastocyst outcomes.

• Journal of Positioning, Navigation, and Timing
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• 제12권3호
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• pp.245-255
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• 2023

Time comparison techniques are necessary for generating and keeping Coordinated Universal Time (UTC) and distributing standard time clocks. Global Navigation Satellite System (GNSS) Common View, GNSS All-in-View, Two-Way Satellite Time and Frequency Transfer (TWSTFT), Very Long Baseline Interferometry (VLBI), optical fiber, and Network Time Protocol (NTP) based methods have been used for time comparison. In these methods, GNSS based time comparison techniques are widely used for time synchronization in critical national infrastructures and in common areas of application such as finance, military, and wireless communication. However, GNSS-based time comparison techniques are vulnerable to jamming or interference environments and it is difficult to respond to GNSS signal disconnection according to the international situation. In response, in this paper, Code-Division Multiple Access (CDMA) based All-to-All TWSTFT operation method is proposed. A software-based simulation platform also was designed for performance analysis in multi-TWSTFT signal environments. Furthermore, code and carrier measurement jitters were calculated in multi-signal environments using the designed simulation platform. By using the technique proposed in this paper, it is anticipated that the TWSTFT-based time comparison method will be used in various fields and satisfy high-performance requirements such as those of a GNSS master station and power plant network reference station.

• Asian-Australasian Journal of Animal Sciences
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• 제24권10호
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• pp.1365-1371
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• 2011

The intensity and duration of sexual behavior in Bos indicus was assessed through the continuous observation of sexual receptivity. Two groups of cows were formed: only synchronized (n = 50) and other group further superovulated (n = 20). An intravaginal implant that released progesterone over 9 d was used. After removing the implant, 25 mg of $PGF_{2{\alpha}}$ was administered. In the superovulated group, the administration of 280 mg (Follicle stimulant hormone) FSH-P1 per cow with a decreasing dosage over 4 d was utilized. In both groups, behavioral observations began at the moment of implant removal. Sexual behavior was analyzed using a Kruskal-Wallis test to compare the mean of hours in estrus, effective mountings and number of mounts/hour during estrus. A nonparametric survival analysis was performed using the time in two ways: i) when an event happened it was placed in a 24 h timeframe and, ii) the time of observation in continuous form (96 h) assessing the difference between curves by the log rank test Chi-square. The only significant difference was the number of mounts/h during receptivity (p<0.05). In the superovulated group three periods of sexual activity during the day were identified, with these events being of greater frequency and duration than the synchronized group (p = 0.02); besides, the superovulated group began estrus before the synchronized group (p = 0.0035) when using the total period. In a simulation study, when the number of observations went from two (06:00-18:00) to three periods (06:00, 12:00 and 18:00) cows detected accurately (<6 h after the onset) increased more than 20%. The results show that superovulated cows presented greater intensity and duration of sexual activity in contrast with only synchronized animals.