The purpose of this study was to evaluate the tensile bond strength between composite resin and the human enamel. Three composite resin systems, two chemical (Clearfil Posterior, and Clearfil Posterior-3) and one light cure (Photo Clearfil-A), used with and without an intermediate resin (clearfil bonding agent), were evaluated under different amounts of load (10g, 200g and 200g for a moment) for in vitro tensile bond strength to acid-eched human enamel. Clinically intact buccal or lingual surfaces of 144 freshly extracted human permanent molars, embedded in acrylic were flattened with No #600 carborundum discs. Samples were randomly assigned to the different materials and treatments using a table of random numbers. Eight samples were thus prepared for each group(Table 2) these surfaces were etched with an acid etchant (Kurarey Co. Japan) in a mode of etching for 30 seconds, washing for 15 seconds, and drying for 30-seconds. During the polymerization of composite resin on the acid-etched enamel surfaces with and without bonding agent 10-gram, 200 gram and temporary 200 gram of load were applied. The specimens were stored in 50% relation humidity at $37^{\circ}C$ for 24 hours before testing. An universal Testing machine (Intesco model No. 2010, Tokyo, Japan) was used to apply tensile loads in the vertical directed (fig 5), and the force required for separation was recorded with a cross head speed of 0.25 mm/min and 20 kg in full scale. The results were as follow: 1. The tensile bond strength was much greater in applying a bonding agent than in not doing that. 2. The tensile bond strength of chemical cure composite resin was higher than that of light cure composite resin with applying on bonding agent on the acid-etched enamel. 3. In case of not applying a bonding agents on the acid-etching enamel, the highest tensile bond strength under 200 gram of load was measured in light cure composite resin. 4. The tensile bond strength under 200-gram of load has no relation with applying the bonding agent. 5. Under the load of 10-gram, There was significant difference in tensile bond strength as applying the bonding agent.
Han Dong-Soo;Kim Hong-Song;Jong Woo-Hyuk;Lee Sung-Doke
Journal of KIISE:Computing Practices and Letters
/
v.11
no.5
/
pp.427-435
/
2005
With the accumulation of protein and its related data on the Internet, many domain based computational techniques to predict protein interactions have been developed. However, most of the techniques still have many limitations to be used in real fields. They usually suffer from a low accuracy problem in prediction and do not provide any interaction possibility ranking method for multiple protein pairs. In this paper, we reevaluate a domain combination based protein interaction prediction method and develop an interaction possibility ranking method for multiple protein pairs. Probability equations are devised and proposed in the framework of domain combination based protein interaction prediction method. Using the ranking method, one can discern which protein pair is more probable to interact with each other than other protein pairs in multiple protein pairs. In the validation of the ranking method, we revealed that there exist some correlations between the interacting probability and the precision of the prediction in case of the protein pair group having the matching PIP(Primary Interaction Probability) values in the interacting or non interacting PIP distributions.
The purpose of this study was to estimate genetic parameters for productive traits in Duroc breed. In this study, 40,657 records for productive traits and the pedigree data of 47,974 families were collected from 41 farms registered at the Korean Animal Improvement Association (KAIA) from 2004 to 2011. The REMLf90 program was used to analyze a multiple traits animal model with fixed effects of sex, contemporary group, parity and age at the end of the test as covariate and random effects of animal and residual error. The heritabilities of days to 90 kg (D90KG), average daily gain (ADG), backfat thickness (BF) and eye muscle areas (EMA) were estimated to be 0.334, 0.340, 0.335, and 0.200, respectively. The genetic correlation coefficients were -0.992 between D90KG and ADG, -0.142 between ADG and BF, -0.361 between ADG and EMA, and -0.243 between BF and EMA. Conversely, positive genetic correlations for D90KG with BF and EMA were 0.13 and 0.36, respectively.
This study was performed to investigate the effect of essential oils and ethanolic extracts of approximately 650 plant species on acetylcholine esterase (AChE) enzyme activity using Ellman's colorimetric method in 96-well microplates. The results showed that the ethanolic extracts from twig of Sophora subprostrata, twig of Phellodendron amurense, seed of Corylopsis coreana, and essential oil (EO) from Citrus paradisi, Cupressus sempervirens, Ocimum basilicum, Pinus sylvestris and Rosmarinus officinalis inhibited more than 80% of AChE activity. Among these, EO from Pinus sylvestris, C. sempervirens and C paradisi exhibited higher values of AChE inhibitory activity, which were 75, 84 and 99% at a concentration of 50 ug/ml, respectively. Finally, EO from C paradisi (grapefruit, GEO) showed the highest inhibitory activity towards AChE, which showed 91% of inhibition at a concentration of 20 ug/ml. We also examined the anti-dementia effects of GEO in mouse by passive avoidance test and Morris water maze test. The model mouse (male, ICR) of dementia (negative control) was induced by administration of scopolamine (1 mg/kg body weight). The latency time of sample group administrated with GEO (100 mg/kg, p.o.) increased significantly as compared with negative control on passive avoidance test. There were significant recovery from the scopolamine-induced deficits on learning and memory in water maze test through daily administrations with GEO (100 mg/kg, p.o.). From these results, we conclude that GEO treatment might enhance the cognitive function, suggesting that the EO of C. paradis may be a potential candidate for improvement of perceptive ability and dementia.
In this study the retrieval algorithms have been developed to retrieve total precipitable water (TPW) from Terra/Aqua Moderate Resolution Imaging Spectroradiometer (MODIS) infrared measurements using a physical iterative retrieval method and a split-window technique over East Asia. Retrieved results from these algorithms were validated against Defense Meteorological Satellite Program (DMSP) Special Sensor Microwave/Imager (SSM/I) over ocean and radiosonde observation over land and were analyzed for investigating the key factors affecting the accuracy of results and physical processes of retrieval methods. Atmospheric profiles from Regional Data Assimilation and Prediction System (RDAPS), which produces analysis and prediction field of atmospheric variables over East Asia, were used as first-guess profiles for the physical retrieval algorithm. We used RTTOV-7 radiative transfer model to calculate the upwelling radiance at the top of the atmosphere. For the split-window technique, regression coefficients were obtained by relating the calculated brightness temperature to the paired radiosonde-estimated TPW. Physically retrieved TPWs were validated against SSM/I and radiosonde observations for 14 cases in August and December 2004 and results showed that the physical method improves the accuracy of TPW with smaller bias in comparison to TPWs of RDAPS data, MODIS products, and TPWs from split-window technique. Although physical iterative retrieval can reduce the bias of first-guess profiles and bring in more accurate TPWs, the retrieved results show the dependency upon initial guess fields. It is thought that the dependency is due to the fact that the water vapor absorption channels used in this study may not reflect moisture features in particular near surface.
The purpose of this study is to examine longitudinal impacts of various factors on happiness in primary school children. Specifically, attachment theory and ecosystems perspective were utilized for the purpose of this study. We used Korea Youth Panel Survey, which is a 5-year longitudinal data collected from fourth grade in elementary school to second grade in middle school Latent growth model was employed as the analytic method. The findings of this study are as follow: first, academic achievement, self-esteem, parent attachment, peer attachment, teacher attachment, and community attachment all decreased over the 5-year study period. Also, the intercept and the slope variance of variables were found to be statistically significant. This means that there are significant differences in the intercept and the slope of individuals. Second, self-esteem, parent attachment, and peer attachment were found to have cross-sectional influences on happiness. This means that self-esteem, parent attachment, and peer attachment are positively associated with happiness at the intercept. Also, self-esteem, parent attachment, teacher attachment, and community attachment were found to have longitudinal influences on happiness. This means that the higher levels of community attachment at the intercept is associated with slower the rate of decrease in happiness at the slope. In addition, faster rates of decrease in self-esteem, parent attachment, teacher attachment, and community attachment are associated with faster the rate of decrease in happiness. Third, this study conducted multiple group analysis with gender. The findings of this analysis revealed no significant differences in analytic models between males and females. Based on these findings, theoretical and practice implications with regard to happiness in primary school children are discussed.
This paper studied condo selection attributes that affected satisfaction, recommendation and revisitation, in particular, investigated gender and age differences. Research target is the group who revisited time-sharing condominium within one year. The paper seeks to understand factors that affect and contribute to customer satisfaction and intentions for reuse. This study model was analyzed by the basic statistical analysis, factor analysis, reliability analysis and multiple analysis, using SPSS 18.0 and AMOS 18.0. We found that 5 condo selection attributes that have significant affect on user satisfaction: facility, service, product, accessibility and expense. Furthermore it was evident that user satisfaction has a significant effect on condo recommendation and intentions of reuse. With regard to sex, for male users expense, accessibility and service had a significant effect on their satisfaction level, while for female users, product was most important. User satisfaction both have a significant effect on recommendation and intentions of reuse but for females this was more evident. Regarding the age, for 20~30 age band, service and product factor had a significant effect on user satisfaction in order, whereas, for the age band of over 40s, expense, product and facility factors were important. User satisfaction of both have a significant effect on recommendation and intentions of reuse. In the meantime user satisfaction of 20~30 age band had a bigger positive significant effect on recommendation and intentions of reuse than the age band over 40s.
The purpose of this study is to provide a comparative evaluation of container port criteria at four major container ports in the Bay of Bengal, including Colombo Port in Sri Lanka, Chennai Port in India, Chittagong Port in Bangladesh and Yangon Port in Myanmar. Important container port selection criteria are identified and comparisons among container ports are made using previous studies, personal interviews and questionnaires, completed by top shipping companies, freight forwarders, logistics service providers, and experts in Myanmar. The AHP method is used to verify the research model and hypothesis. This study identified five main criteria and eleven sub-criteria when choosing potential regional hub ports among the four ports in the Bay of Bengal. The main findings from the five main criteria suggest that port efficiency is the highest priority criteria, and the second priority is port costs. The criteria of geographical location and available port facilities are the third and fourth most important, respectively, and the last priority is port's hinterland. Regarding the relative competition among these ports, Colombo Port obtained the highest priority among the four influential factors except for port hinterland. This study has certain limitations that will require future research. First, the sample group for the population size is relatively small. Second, interviewees had limited experience answering questionnaires using this methodology and a limited amount of time was available for respondents for the interviews.
Rhodosporidium toruloides has been known as an in vitro model of fat metabolism. The purpose of this study is to identify the triglyceride inhibitory effects of hot water extracts from beetroot using R. toruloides. The triglyceride content of oleaginous yeast cultured from YPD culture medium were regulated by treatment of beet root hot water and ethanol extract, respectively. In addition, the number of cells in the oleaginous yeast was 10.48, 8.46, 12.40, 12.80 and $8.24{\times}10^3cell/mL$. The treatment of hot water extract of beet root increased total lipid content of oleaginous yeast in dose dependently. Moreover, the triglyceride content of oleaginous yeast was decreased by hot water extract of beet root extract, respectively. The fat droplet in the oleaginous yeast decreased according to the concentration of hot water extracts from beetroot. The ratio of increase in the number of cells in the oleaginous yeast were increased dose-dependently by treatment of hot water extract from beetroot compared with control group. The free fatty acid and total carotenoid contents were increased concentration-dependently by treatment of hot water extracts from beetroot. These study results indicate that hot water extracts from beetroot has a triglyceride inhibitory effects.
In the present study, we have investigated the effects of centrally administered ginsenoside Rc or Rgl on the modulation of NMDA receptor and $GABA_A$ receptor binding in rat brain. The NMDA receptor binding was analyzed by quantitative autoradiography using $[^3H]MK-801$ binding, and $GABA_A$ receptor bindings were analyzed by using $[^3H]muscimol\;and\;[^3H]flunitrazepam$ in rat brain slices. Rats were infused with ginsenoside Rc or Rg1 ($10\;{\mu}g/10{\mu}l/hr$, i.c.v.) for 7 days, through pre-implanted cannula by osmotic minipumps (Alzet, model 2ML), The levels of $[^3H]MK-801$ binding were highly decreased in part of cortex and cingulated by ginsenoside Rc and Rgl. The levels of $[^3H]muscimol$ binding were strongly elevated in almost all regions of frontal cortex by the treatment of ginseoside Rc but decreased by ginsenoside Rg 1. However, the $[^3H]flunitrazepam$ binding was not modulated by ginsenoside Rc or ginsenoside Rgl infusion. These results suggest that prolonged infusion of ginsenoside could differentially modulate $[^3H]MK-801\;and\;[^3H]muscimol$ binding in a region-specific manner. Also, we investigated the influence of centrally administered ginsenoside on the regulation of mRNA levels of the family of NMDA receptor subtypes (NR1, NR2A, NR2B, NR2C) by in situ hybridization histochemistry in the rat brain. The level of NR1 mRNA is significantly increased in temporal cortex, caudate putamen, hippocampus, and granule layer of cerebellum in Rgl-infused rats as compared to control group. The level of NR2A mRNA is elevated in the frontal cortex. In contrast, it was decreased in CAI area of hippocampus in Rgl-infused rats. However, there was no significant change of NR1 and NR2A mRNA levels in Rc-infused rats. The level of NR2B mRNA is elevated in cortex, caudate putamen, and thalamus in both Rc- and Rg-infused rats. In contrast, NR2B level is decreased in CA3 in Rgl-infused rats. The level of NR2C mRNA is increased in the granule layer of cerebellum in only Rg1 but not Rc infused rats. These results show that structure difference of ginsenoside may diversely affect the modulation of expression of NMDA receptor subunit mRNA after infusion into cerebroventricle in rats.
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