• BMB Reports
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• 제47권5호
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• pp.241-248
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• 2014

CD1 molecules belong to non-polymorphic MHC class I-like proteins and present lipid antigens to T cells. Five different CD1 genes (CD1a-e) have been identified and classified into two groups. Group 1 include CD1a-c and present pathogenic lipid antigens to ${\alpha}{\beta}$ T cells reminiscence of peptide antigen presentation by MHC-I molecules. CD1d is the only member of Group 2 and presents foreign and self lipid antigens to a specialized subset of ${\alpha}{\beta}$ T cells, NKT cells. NKT cells are involved in diverse immune responses through prompt and massive production of cytokines. CD1d-dependent NKT cells are categorized upon the usage of their T cell receptors. A major subtype of NKT cells (type I) is invariant NKT cells which utilize invariant $V{\alpha}14-J{\alpha}18$ TCR alpha chain in mouse. The remaining NKT cells (type II) utilize diverse TCR alpha chains. Engineered CD1d molecules with modified intracellular trafficking produce either type I or type II NKT cell-defects suggesting the lipid antigens for each subtypes of NKT cells are processed/generated in different intracellular compartments. Since the usage of TCR by a T cell is the result of antigen-driven selection, the intracellular metabolic pathways of lipid antigen are a key in forming the functional NKT cell repertoire.

• Archives of Pharmacal Research
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• 제29권7호
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• pp.598-604
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• 2006

In many conventional drug delivery systems in vogue, failure to deliver efficient drug delivery at the target site/organs; is evident as a result, less efficacious pharmacological response is elicited. Microspheres can be derived a remedial measure which can improve site-specific drug delivery to a considerable extent. As an application, Lung-targeting Ofloxacin-loaded gelatin microspheres (GLOME) were prepared by water in oil emulsion method. The Central Composite Design (CCD) was used to optimize the process of preparation, the appearance and size distribution were examined by scanning electron microscopy, the aspects such as in vitro release characteristics, stability, drug loading, loading efficiency, pharmacokinetics and tissue distribution in albino mice were studied. The experimental results showed that the microspheres in the range of $0.32-22\;{\mu}m$. The drug loading and loading efficiency were 61.05 and 91.55% respectively. The in vitro release profile of the microspheres matched the korsmeyer’s peppas release pattern, and release at 1h was 42%, while for the original drug, ofloxacin under the same conditions 90.02% released in the first half an hour. After i.v. administration (15 min), the drug concentration of microspheres group in lung in albino mice was $1048\;{\mu}g/g$, while that of controlled group was $6.77\;{\mu}g/g$. GLOME found to release the drug to a maximum extent in the target tissue, lungs.

• 생약학회지
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• 제2권1호
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• pp.23-28
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• 1971

This study was planned to see whether the Panax Ginseng root has any influence upon the emotionality in rats exposed to a novel open-field and induced by a grid shock. Sixty four male albino rats whose body weight ranged from 130 to 180 gm were used. They were divided into four groups, i.e., placebo control group, 5mg/kg Ginseng ext. group, 10mg/kg Ginseng ext. group, and 20mg/kg Ginseng ext. group. Sixteen rats were subjected in each group. Open-field activity was measured in a $2.20m\;{\times}\;2.20m$ open-field that was divided into 15cm squares. Four novel objects and food pellets were put with an equal distance in the open-field. Locomotion was recorded by entering the number of squares traversed for 4 min. and behaviors which were shown at the 10-sec. period were checked by a modified Jarrad and Bunnel's observation procedure. Sensitivity to the grid shock was measured in a compartment of shuttle box. 10, 20, 30, 40, 50, 60 and 70 volt ac. 2 sec., electric shock were delivered in each subject by the grid to animal's feet. Flinch, vocalization and jumping responses were rated in each shock level. The results obtained were as follows: 1.) 5mg/kg and 10mg/kg Ginseng ext. groups were tended to increase the ambulation and exploration compared with placebo control. 2.) All Ginseng extract groups were defecated significantly less often than placebo control in the open-field and shock situation. 3.) Thresholds to the electric shock were tended to elevate in all Ginseng groups. 4.) These results suggest that Ginseng extract has a stimulant action in open-field and a tranqulizing action in feared situation.

• 정보처리학회 논문지
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• 제13권2호
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• pp.34-40
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• 2024

비밀공유 기법은 개인키와 같은 비밀을 복수의 지분으로 분할하여 분산 관리함으로써 비밀의 보안성을 높이는 기술이다. 그동안 다양한 상황에서 비밀공유를 적용하기 위한 많은 연구가 있어 왔으며, Tassa가 제안한 논리곱 기반의 비밀공유 방법은 도함수를 사용하여 계층적 비밀공유를 가능하게 하는 방법이다. 하지만 도함수를 사용하는 계층적 비밀공유는 몇 가지 한계를 가진다. 첫째, 각 레벨의 지분들이 하나의 도함수로부터 생성되기 때문에 하나의 레벨에 하나의 참여자 그룹만을 만들 수 있다. 둘째, 논리곱에 기반한 비밀 복원만 가능하여 임의의 비밀 복원 조건을 규정할 수 없다. 셋째, 도함수를 사용하기 때문에 버크호프 보간법을 필요로 하며, 이는 다항식 기반 비밀공유에 사용되는 라그랑주 보간법에 비해 구현이 복잡하고 어렵다. 본 논문에서는 논리곱 기반 계층적 비밀공유를 일반화시킨 다중 컴파트먼트 비밀공유 기법을 제안한다. 제안하는 기법은 비밀을 복원하는데 필요한 외부지분들을 이용하여 비밀을 암호화하고, 암호화된 비밀 값이 삽입된 다항식을 생성하여 내부지분들을 생성한다. 내부지분들로 다항식을 복원할 수는 있지만, 이 때 얻을 수 있는 값은 암호화된 비밀 값이며 복호화를 위해서는 외부지분들이 필요하다. 이 기법을 적용하면 하나의 계층에 복수의 참여자 그룹을 만들 수 있으며, 논리곱은 물론 임의의 비밀 복원 조건을 구현할 수 있다. 또한 다항식을 사용함에 따라 라그랑주 보간법을 적용하는 것도 가능해진다.

• 대한치과교정학회지
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• 제18권1호
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• pp.189-207
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• 1988

In almost all biologic systems, mechanically induced electric charge separation is a fundamental phenomenon. Since the hypothesis was established that the generation of electric potentials in bone by mechanical stress including muscular force might control the activity in bone by mechanical stress including muscular force might control the activity of osseous cells and their biopolymeric byproduct, the concept of electrically mediate growth mechanism, which involves biological growth and bone remodeling by any means, in living systems has been applied clinically and experimentally to orthopedic fracture repair, the regulation of orthodontic tooth movement, epiphyseal cartilage regeneration, etc. On the other hand, recent numerous research data available show apparently that the mandibular condyle has the characteristics of growth center as well as growth site. In addition, there exists a considerable difference of opinion as to the role of external pterygoid muscle in condylar growth. In view of these evidences, this. experiment was performed to investigate the effect of the galavic current on the growth of the mandible and condyle for elucidating the nature of condylar growth. The bimetallic device was composed of silver and platinum electrode connected with resistor (3.9 Mohm), which was expected to produce galvanic current of 23.6 nA according to the galvanic principle. The 25 Sprague-Dawley rats were divided into two group, 2 week group comprising 8 animals exposed to satanic current for 2 weeks and 3 control animals not exposed for 2 weeks, 4 week group comprising 10 animals in experimental group and 4 animals in control group applied for 4 weeks respectively. The experimental rats were subjected to application of the galvanic current invasively to codylar head surface and the control groups with sham electrode. On the basis of anatomic and histologic data from the mandibular condyle of experimental and control group, the following results were obtained. 1. After 2 weeks, there was no increase of mandibular size in experimental group over that of the control group. 2. After 4 weeks, the size of the condylar head was larger in experimental group than that of the control. 3. In 2 week group, the thickness of the mitotic compartment and hypertrophic chondroblastic layer was increased in experimental group. 4. In 4 week group, the number and the size of the hypertrophic chondroblasts were increased significantly on experimental group over that of the control group. 5. The application of the satanic current caused an increase in chondrocytic hypertrophy and intercellular matrix in both groups.

• Animal cells and systems
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• 제7권4호
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• pp.303-307
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• 2003

The aim of this study is to investigate whether alcohol alters learning and memory processes pertaining to emotional and spatial factors using the active avoidance and T-maze task in zebrafish. In the active avoidance task, zebrafish were trained to escape from one compartment to another to avoid electric shocks (unconditioned stimulus) following a conditioned light signal. Acquisition of active avoidance task appeared to be normal in zebrafish that were treated with 1% alcohol for 30 min for 17 days until the end of the behavioral test, and retention ability of learned behavior, tested 2 days later, was the same as control group. In the T-maze task, the time to find a reservoir was compared. While the latency was similar during the 1 st training session between control and alcohol-treated zebrafish, it was significantly longer in alcohol-treated zebrafish during retention test 24 h later. Furthermore, when alcohol was treated 30 min after 2nd session without prior treatment, zebrafish demonstrated similar retention ability compared to control. These results suggest that chronic alcohol treatment alters spatial learning of zebrafish, but not emotional learning.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권5호
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• pp.453-457
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• 2006

This study was designed to investigate the effect of intra-articular injection of hyaluronic acid for the treatment of internal derangements of the temporomandibular joint(TMJ). Sixteen patients with internal derangements of TMJ in 1 male and 15 females aged 68 to 18 years comprised the study material. The patients' complaint was limited mouth opening and pain during function. Patients were divided into 3 groups(articular disc displacement with reduction, articular disc displacement without reduction, osteoarthritis group). The preauricular area was disinfected anesthetized locally with 2% lidocaine hydrochloride. Arthrocentesis was performed. Hyaluronic acid(1.5 ㏄) was then injected into the superior compartment of the TMJ. Active range of motion exercises were instituted at approximately 24 hours postoperatively. Antibiotics and NSAID, three times daily by mouth, was prescribed for 3 days. Clinical evaluation of the patients was done before the procedure, immediately after the procedure, final follow-up visit postoperatively. Pain during function was assessed using visual analog scales(VAS). Maximal mouth opening was recorded as a distance between upper incisal edge and lower incisal edge. Intra-articular injection of hyaluronic acid caused significant reduction of pain during function, significant increase of maximal opening range. These findings suggest that intra-articular injection of hyaluronic acid is useful for decreasing patient reports of pain while increasing functional mobility of the mandible in internal derangements of the TMJ.

• Journal of Applied Biological Chemistry
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• 제58권4호
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• pp.363-368
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• 2015

The pharmacokinetics of florfenicol were studied in healthy and vibriosis-infected large yellow croaker (Pseudosciaena crocea) following administration of a single oral dose of $20mg{\cdot}kg^{-1}$ at $25{\pm}2^{\circ}C$. After oral administration, florfenicol levels in tissues (liver, kidney, muscle, serum, and skin) were analyzed using high-performance liquid chromatography. A two-compartment open model was used to describe the pharmacokinetics of florfenicol following oral administration. Compared to the healthy group, the absorption rate of vibriosis-infected fish significantly decreased, peak-time ($T_{max}$) delayed, maximum concentration ($C_{max}$) declined, total body clearance decreased, the elimination half-life ($T_{1/2{\beta}}$) was extended, and the area under the curve increased. These results indicate that a $20mg{\cdot}kg^{-1}$ oral dose of florfenicol administered once daily continuously for 4 or 5 days can be used for the treatment of Vibrio alginolyticus infection in large yellow croaker (Pseudosciaena crocea).

• 한국CDE학회논문집
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• 제4권4호
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• pp.381-390
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• 1999

In this study, a pseudo ship structure data model for the :.hip cargo hold structure based on STEP is proposed. The proposed data model is based on Application Reference Model of AP218 Ship Structure which is the model that specifies conceptual structures and constraints used to describe the information requirements of an application. And the proposeddata model refers the Ship Common Model framework for the model architecture which is the basis for ongoing ship AP development within the ISO ship-building group and the ship product definition information model of CSDP research project for analyzing the relationship between ship structure model entities. The proposed data model includes Space, Compartment. Ship Structural System, Structural Part and Structural Feature of cargo hold. To generate this data model schema in EXPRESS format, ‘GX-Converter’was used which enables user to edit a model in EXPRESS format and convert schema file in EXPRESS format. Using this model schema, STEP physical file containing design data for ship cargo hold data structure was generated through SDAI programming. The another STEP physical file was also generated containing geometry data of ship cargo hold which was extracted and calculated by SDAI and external surface/surface intersection program. The geometry information of ship cargo hold can be then transferred to commercial CAD system, for example, Pro/Engineer. Examples of the modification of the design information are also Presented.

• Animal cells and systems
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• 제5권3호
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• pp.263-266
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• 2001

Metallothionein (MT) is induced in the regenerating rat liver. We have investigated expression of MT gene by RT PCR as well as specific localization of MT by immunocytochemistry in regenerating rat liver after partial hepatectomy (PH). MT mRNA level started to increase from 1 h and reached the peak at 8 h after PH. The level decreased gradually by 24 h, and became similar to that of control group. In the immunocytochemical study, in all groups treated with primary antibody, immunogold particles indicating the presence of MT were evenly distributed throughout both cytoplasm and nucleus of the rat hepatocytes. Within the nucleus, the gold particles appeared to be intensely localized in the areas of euchromatin and nucleolus. Within the cytoplasm, gold particles did not seem to adhere to mitochondria or Iysosomes, but were freely distributed. However, rough endoplasmic reticulum was the obvious compartment on which the gold particles were localized. Time course of MT immunoreactivity revealed that distribution of gold particles in hepatocytes increased gradually by 24 h, and decreased at 48 h after PH. Briefly, PH resulted in the sharpest increase in the expression of MT mRNA at 8 h and in the immunoreactivity of MT at 24 h, respectively. It is suggested that the increase of MT mRNA expression, the intensity of immunoreactivity and the specific localization of MT may be associated with the compensatory cell proliferation followed by PH.