• Food Science and Biotechnology
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• 제14권3호
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• pp.344-349
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• 2005

Aldose reductase catalyzes the conversion of glucose into sorbitol. Inhibiting this enzyme in diabetes mellitus can delay or prevent pathogenic process. Aldose reductase inhibitor was screened from Korean fermented soybean pastes (Doen-jang) and purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. Aldose reductase inhibitor was identified as genistein with molecular weight of 270 Da and molecular formula of $C_{15}H_{10}O_5$ based on UV spectrometry, $^1H$ and $^{13}C\;NMRs$, and mass spectrometry. Genistein inhibited aldose reductase of pig lens with $IC_{50}$ level of $20\;{\mu}M$. Because genistein was effective against aldose reductase of animal source, it may be a potential therapeutic agent for diabetic complications.

• 한국작물학회지
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• 제55권3호
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• pp.187-194
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• 2010

고품질 녹색자엽 검정콩 신품종 육성 연구의 일환으로 녹색자엽의 유전자원별 녹색의 정도를 평가할 수 있는 엽록소 함량 및 조성과 이들의 유전양상을 검토하고, 유전적 배경을 달리한 녹색자엽 자원을 활용한 녹색자엽 검정콩의 선발 효율을 검토한 결과를 요약하면 아래와 같다. 1. 유전적 배경이 상이한 녹색자엽 자원을 검정콩 육종프로그램에 도입코자 실시한 특성조사 결과 핵유전 녹색자엽 유전자 d1d2를 보유한 자원은 세포질 유전 녹색자엽 유전자 cytG를 보유한 자원보다 총 엽록소의 함량이 매우 높은 경향이었고, 조성에서는 d1d2 보유 자원은 엽록소 a의 함량이 엽록소 b보다 2배 이상 높고, cytG 보유 유전자원은 엽록소 b의 함량이 엽록소 a 함량 보다 높은 경향이었다. 2. 핵유전 녹색자엽 유전자(d1d2)는 당대의 종실에서 발현되어 $F_1$ 세대의 $F_2$ 종실에서부터 선발이 가능하며, 2개의 열성유전자에 지배를 받는다. 3. 녹색 종피의 핵유전 녹색자엽(d1d2)과 황색자엽 검정콩(D1D2)의 교배후대에서 검정색 종피에 녹색자엽 종자를 가진 개체는 모본의 유전적 배경에 따라 조금은 다르지만 $F_2$ 전체 개체 중 3% 미만의 낮은 비율로 나타나, 향후 핵유전 녹색자엽 검정콩 신품종 육성효율 증진을 위해서는 핵유전 녹색자엽 검정콩 중간모본 개발과 선발방법 개선이 필요할 것으로 판단된다.

• Journal of Biosystems Engineering
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• 제41권2호
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• pp.126-137
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• 2016

Purpose: The overall objective of this study was to evaluate the vegetation fraction of soybeans, grown under different cropping conditions using an unmanned aerial vehicle (UAV) equipped with a red, green, and blue (RGB) camera. Methods: Test plots were prepared based on different cropping treatments, i.e., soybean single-cropping, with and without herbicide application and soybean and barley-cover cropping, with and without herbicide application. The UAV flights were manually controlled using a remote flight controller on the ground, with 2.4 GHz radio frequency communication. For image pre-processing, the acquired images were pre-treated and georeferenced using a fisheye distortion removal function, and ground control points were collected using Google Maps. Tarpaulin panels of different colors were used to calibrate the multi-temporal images by converting the RGB digital number values into the RGB reflectance spectrum, utilizing a linear regression method. Excess Green (ExG) vegetation indices for each of the test plots were compared with the M-statistic method in order to quantitatively evaluate the greenness of soybean fields under different cropping systems. Results: The reflectance calibration methods used in the study showed high coefficients of determination, ranging from 0.8 to 0.9, indicating the feasibility of a linear regression fitting method for monitoring multi-temporal RGB images of soybean fields. As expected, the ExG vegetation indices changed according to different soybean growth stages, showing clear differences among the test plots with different cropping treatments in the early season of < 60 days after sowing (DAS). With the M-statistic method, the test plots under different treatments could be discriminated in the early seasons of <41 DAS, showing a value of M > 1. Conclusion: Therefore, multi-temporal images obtained with an UAV and a RGB camera could be applied for quantifying overall vegetation fractions and crop growth status, and this information could contribute to determine proper treatments for the vegetation fraction.

• 한국식품과학회지
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• 제30권4호
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• pp.883-887
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• 1998

육류 조리에 사용되거나 또는 육류 섭취시 부식으로 제공되는 농산물을 위주로 단백분해효소의 활성을 측정한 결과, 청양고추, 꽈리고추, 깻잎, 콩나물, 숙주나물에서 높은 효소 활성을 보였으며, 이중 콩나물의 단백질분해 활성이 가장 높았다. 또한 효소의 사용시 중요한 지표인 효소의 안정성을 투석과 동결 해동 후 잔존 효소활성을 측정하였다. 투석 후 숙주나물, 청양고추, 꽈리고추와 깻잎은 12, 23, 45%와 37%의 잔존 활성을 보였으며, 콩나물은 64%의 잔존 활성을 보임에 따라 비교적 다른 효소에 비해 안정한 것을 확인하였다. 동결 해동 후 숙주나물과 콩나물의 단백분해효소는 100%와 65%의 높은 잔존 활성을 보였다. 비교적 효소의 활성이 높고 또한 안정성이 우수한 콩나물의 단백분해효소의 활성은 소고기보다는 돼지고기에 대한 분해 효과가 우수하였으며, stroma protein보다는 myofibrillar과 sarcoplasmic protein에 대한 분해력이 우수하였다. SDS-PAGE에 의한 육 단백의 분해 특성은 myosin heavy chain, actin, tropomyosin과 myosin light chain이 관찰되었으며, 반응시간이 경과할수록 myosin heavy chain, actin, tropomyosin이 분해되는 경향을 보였다. 특히 2시간 효소처리 시 근원섬유 단백질의 분해 효과가 뛰어났다. 육 단백의 농도가 증가할수록 단백분해 활성은 완만히 증가하다가 평형을 이루는 경향을 보였다.

• 한국유기농업학회지
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• 제7권1호
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• pp.79-90
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• 1998

제주지역에서 前作物로 綠肥作物을 재배하여 破碎$\cdot$耕耘하고 폴리에칠렌 투명비닐로 1997년 8월 5일부터 8월 29일까지 太陽熱土壤消毒후 가을감자를 재배한 결과를 요약하면 다음과 같다. 멀칭기간중의 토양깊이 10cm에서 최고지원은 $42^{\circ}C$였다. 綠肥作物栽培 및 멀칭에 의해 토양의 pH 및 有機物含量은 높아졌다. 前作物로 재배한 綠肥作物의 生體收量은 sudangrass 交雜種, 콩, 알팔파 순으로 많앙Tejs 반면 멀칭 太陽熱土壤消毒후 가을감자의 地上部生體重, 塊莖收量 등의 생육형질은 콩 + 가을감자재배구가 가장 양호한 것으로 나타났다. 이상의 결과로 볼 때 여름철 고온기에 綠肥作物栽培후 비닐 피복 太陽熱消毒에 의해 작물의 생육형질이 향상될 뿐만 아니라 土壤消毒의 가능성도 있을 것으로 생각된다.

• Bulletin of the Korean Chemical Society
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• 제34권7호
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• pp.2044-2050
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• 2013

A series of poly(glycerol-succinic acid) dithiocarbamate and 1,3,4-thiadiazole dendrimers, which have potential as anti-wear oil additives, were synthesized. Their anti-wear properties in three different oils (100N, DB-51, and soybean) were evaluated using a four-ball wear tester. The results indicated that thiocarbamate dendrimers have moderate anti-wear properties in DB-51 oil, and 1,3,4-thiadiazole dendrimers exhibited good anti-wear properties in 100N and DB-51 oils. However, dithiocarbamate and 1,3,4-thiadiazole dendrimers were not effective anti-wear additives in soybean oil.

• Mycobiology
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• 제40권4호
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• pp.258-262
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• 2012

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces $H_2O_2$ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of $H_2O_2$ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to $150{\mu}M$ within 90 min.

• 한국조리학회지
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• 제3권
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• pp.327-341
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• 1997

Food materials usually contain much water, and thus are susceptible to decay be enzyme and microorganism. Cereals are usually consumed as staple food, but they have low protein content. Protein is a major component of body, and should be consumed as a nutrient for all ages. Animal protein like chicken and vegetable protein such as bean curd and soybean and good sources of high quality protein. Bean curd is a traditional food which have good nutrients, and so various cooking methods are needed to be developed for the supplement of high quality food. 1) Cereals such as rice, barley, and bean curd, soybean, green vegetables, tangle and brown seaweed are known as food for long life. 2) Soybean is able to suppress the formation of hydroperoxide by saponin and lecithin. 3) Curd in bean curd means soft in Chinese character, and bean curd may be the mother of cheese. 4) Bean curd have high nutrition and digestibility. As soybean is dipped longer in water for bean curd, the production rate is higher. 5) There are many kinds of bean curd products, and can be purchased inexpensively.

• 한국작물학회지
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• 제49권2호
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• pp.141-147
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• 2004

This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

• Preventive Nutrition and Food Science
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• 제7권3호
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• pp.255-260
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• 2002

Yukwa is a popular Korean traditional fried rice snack. The high fat content and porous structure of Yukwa cause it to rapidly become rancid, presenting difficult challenges for commercial distribution. In this study, an-tioxidant activities of green tea extracts (GTE) were evaluated in Yukwa fried in soybean oil (SBO), rice bran oil (RBO) and winterized rice bran oil (WRBO) during storage at 4$0^{\circ}C$ for 12 weeks. Lipid oxidation of Yukwa was determined by acid value (AV), peroxide value (POV), p-anisidine value(AnV), totox value and sensory evaluation. The addition of GTE to the oils reduced the increases in AV, POV, AnV, and totox. Totox increased most vapidly in Yukwa fried in SBO, fellowed by RBO>WRBO>SBO+200 ppm GTE>RBO+200 ppm GTE > WRBO + 200 ppm GTE (p<0.05). Sensory evaluation revealed that the addition of 200 ppm GTE delays rancidity in Yukwa by 7~8 weeks; providing compelling evidence that GTE is an effective antioxidant for Yukwa.