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http://dx.doi.org/10.5941/MYCO.2012.40.4.258

Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green  

Son, Yu-Lim (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Kim, Hyoun-Young (Institute of Sunchang Fermented Soybean Products)
Thiyagarajan, Saravanakumar (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Xu, Jing Jing (Institute of Sunchang Fermented Soybean Products)
Park, Seung-Moon (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Publication Information
Mycobiology / v.40, no.4, 2012 , pp. 258-262 More about this Journal
Abstract
cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces $H_2O_2$ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of $H_2O_2$ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to $150{\mu}M$ within 90 min.
Keywords
Dye decolorization; Glyoxal oxidase; Manganese peroxidase; Phanerochaete chrysosporium; Pichia pastoris;
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