• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.187-201
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• 2005

Bioprocessing technologies utilizing 'biorecognition' between a solid matrix and a protein is being widely experimented as a means to replacing the conventional, solution-based technology. Frequently the matrices are chromatographic resins with specific functional groups exposed outside. Since the reactions of and interactions with the proteins occur as they are attached to the solid matrix, this 'solid-phase' processing has distinct advantages over the solution-phase technology. Solid-phase refolding of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed from the micromoiety around the protein, it is refolded into a native, three-dimensional structure. Once the refolding is complete, the folded protein can be eluted by a conventional elution technique such as the salt-gradient. This concept was successfully extended to 'EBA (expanded bed adsorption)-mediated refolding,' in which the denaturant-dissolved inclusion body in whole cell homogenate is adsorbed to a Streamline resin while cell debris and other impurity proteins are removed by the EBA action. The adsorbed protein follows the same refolding steps. This solid-phase refolding process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this paper, the experimental results of the solid-phase refolding technology applied to several biopharmaceutical proteins of various types are presented.

• Journal of the Korean Geotechnical Society
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• v.28 no.12
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• pp.5-15
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• 2012

In this study, a series of CFD (Computational Fluid Dynamics) numerical analyses were performed in order to evaluate the thermal performance of six full-scale closed-loop vertical ground heat exchangers constructed in a test bed located in Wonju. The circulation HDPE pipe, borehole and surrounding ground formation were modeled using FLUENT, a finite-volume method (FVM) program, for analyzing the heat transfer process of the system. Two user-defined functions (UDFs) accounting for the difference in the temperatures of the circulating inflow and outflow fluid and the variation of the surrounding ground temperature with depth were adopted in the FLUENT model. The relevant thermal properties of materials measured in laboratory were used in the numerical analyses to compare the thermal efficiency of various types of the heat exchangers installed in the test bed. The simulation results provide a verification for the in-situ thermal response test (TRT) data. The CFD numerical back-analysis with the ground thermal conductivity of 4 W/mK yielded better agreement with the in-situ thermal response tests than with the ground thermal conductivity of 3 W/mK.

• Journal of the Korean Electrochemical Society
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• v.14 no.1
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• pp.1-8
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• 2011

Ionic liquids are room-temperature molten salts, composed of organic mostly of organic ions that may undergo almost unlimited structural variation. We approach the new aspects of ionic liquids in applications where the semiconductor nanoparticles used as sensitizers of solar cells. We studied the effects of ionic liquids as capping ligand and/or solvent, on the morphology and phase of the CdSe/ZnS nanoparticles. Colloidal CdSe/ZnS nanoparticles were synthesized using a series of imidazolium ionic liquids; 1-R-3-methylimidazolium bis(trifluoromethylsulfonyl) imide ([RMIM][TFSI]), where R = ethyl ([EMIM]), butyl ([BMIM]), hexyl ([HMIM]), octyl ([OMIM]). The average size of nanoparticles was 8~9 nm, and both zinc-blende and wurtzite phase was produced. We also synthesized the nanoparticles using a mixture of trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl)imide ([$P_{6,6,6,14}$][TFSI]) and octadecene (ODE). The CdSe/ZnS nanoparticles have a smaller size (5.5 nm) than that synthesized using imidazolium, and with a controlled phase from zinc-blende to wurtzite by increasing the volume ratio of [$P_{6,6,6,14}$][TFSI]. For the first time, the phase and size control of the CdSe/ZnS nanoparticles was successfully demonstrated using those ionic liquids.

• Resources Recycling
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• v.19 no.1
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• pp.33-39
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• 2010

The spheric sintered body with $6{\pm}2mm$ diameter was manufactured in a rotary kiln at $1125^{\circ}C$/15 min using green body formed by pelletizing the batch powder composing of coal bottom ash produced from power plant and dredged soil by 70:30, wt%. And the physical properties of sintered body (BD) were analyzed to confirm the possibility for applying to an absorbent to restore a contaminated soil. The sintered body had a giant pore above 100 ${\mu}m$ and a fine pore below 10 ${\mu}m$, and bulk density was 1.4. Also its specific surface area, porosity and void proportion were $12.0m^2/g$, 30.1% and 38.2% respectively. The crushed body (BD-C), produced by crushing a BD specimen into an irregular shape with a aspect ratio of about 2, was similar to BD specimen at bulk density and pore size distribution. But it had superior values of specific surface area, porosity and void proportion compared with BD specimen owing to a decreased apparent volume due to conversion of closed pore existed at interior of BD to open pore during a crushing process. The IEP of sintered body occurred at about pH=5, so the optimum pH condition of reacting aqueous solution could be known before bonding a microbe to the sintered body. Hence, the optimum void proportion and porosity of an absorbent can be obtained by appropriate mixing a BD with BD-C from the base data calculated in this study.

• Journal of the Korean Institute of Landscape Architecture
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• v.44 no.4
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• pp.109-120
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• 2016

This study aims to assess vulnerability in urban thermal environments of Seoul by using indicators frequently used in previous studies as well as effective indicators for spatial planning like urban and environmental planning. For this purpose, fifteen indicators that influence urban thermal environments such as heat waves, urban heat island effects, and urban micro-climates were identified based on literature reviews. Indicators for presenting urban structure and spatial properties were included; for example, building volume as 'exposure to climate', buildings completed before 1980 as 'sensitivity', and green space areas as 'adaptive capacity'. Among them, twelve indicators were applied to assess vulnerability in urban thermal environments of Seoul by using a GIS spatial analysis combined with fuzzy logic. The results show that the Gangnam area is identified as more vulnerable to a heat environment as compared to the Gangbuk area. In the Gangnam area, Seocho-gu, Gangnam-gu, Dongjak-gu, Yeongdeungpo-gu, Gangseo-gu were relatively high in vulnerability, while Dongdaemun-gu, Gangbuk-gu, Gwangjin-gu, Jungrang-gu were relatively high in the Gangbuk area. Gwanak-gu, Dobong-gu, Eunpyeong-gu, and Nowon-gu, which include forested areas, have low vulnerability in the sectors of 'exposure to climate' and 'sensitivity' due to the impact of Gwanaksan and Bukhansan. However, some areas with high vulnerability like Seocho-gu and Gangnam-gu may have lower vulnerability if the indicator 'status of air conditioning' from the sector of 'adaptive capacity' is used. This study could support the establishment of a practicable thermal environment policy and spatial planning to reduce heat-related risks in the field of urban and environmental planning.

• Journal of the Korean Society of Physical Medicine
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• v.9 no.2
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• pp.151-159
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• 2014

PURPOSE: The purpose of this study was to evaluate whether light-emitting diodes (LED) irradiation could be effective in a noninvasive, therapeutic device for the treatment of osteoarthritis(OA). METHODS: Twenty-four male Sprague-Dawley rats were divided into four groups: Vehicle control (saline); monosodium iodoacetate-injection (MIA); LED irradiation after MIA injection (MIA-LED); indomethacin-treatment after MIA injection (MIA-IMT). OA was induced by intra-articular injection of 3 mg MIA through the patellar ligament of the right knee. Vehicle control rats were injected with an equivalent volume of saline. The LED was irradiated for 15 min/day for a week after 7 days of MIA treatment. To compare with the effect of LED irradiation, the indomethacin was administrated 20 mg/kg twice a week orally after 7 days of MIA treatment. Knee joints were removed and fixed overnight in 10% neutral buffered formalin and decalcified by EDTA for 2 week before being embedded in paraffin. The assessment of OA induction were monitored by knee movement and radiographic finding. Histologic analysis were performed following staining with hematoxylin and eosin, safranin O-fast green, or toluidine blue, picrosirius red, and histologic changes were scored according to a modified Mankin system. Apoptotic cell in tissue sections was detected using TUNEL method. RESULTS: Radiographic examination could not show the differences between the MIA-treated and the MIA-LED-treated rats. In the histologic analysis, however, LED irradiation prevented cartilage damage and subchondral bone destruction, and significantly reduced mononuclear inflammatory cell infiltration and pannus formation. LED irradiation also reduced apoptosis of cartilage cells, but it prevented apoptosis of infiltrated inflammatory cells in synovium. In addition, LED irradiation showed an increase of collagen production in the meniscus. CONCLUSION: These results suggest that the 840 nm LED irradiation would be a suitable non-thermal phototherapy for the treatment of OA, as a cartilage protection and anti-inflammatory modality.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.271-278
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• 2009

This study was conducted to investigate the effect of the mixture ratio of the inorganic soil amendments on the soil physicochemical properties. In this experiment, three kinds of soil amendments which had similar pH, EC and particle size, the A, B and C, were tested. The mixture ratio of soil amendment were 0%, 3%, 5%, 7% and 10% (V/V) incorporated with sand which met to the USGA(United State of Golf Association) particle standard. To analyze the effects of amendment on chemical soil properties, pH, EC(electrical conductivity) and CEC(cation exchangeable capacity) were measured. The porosity, bulk density and hydraulic conductivity also measured to analyze the changes of physical properties. In the chemical properties, pH was significantly related to the mixture ratios of amendments, A and C(P<0.05), CEC and EC also related to the ratios of C(P<0.01). When the results were applied to the USGA standard of the soil physical properties, the optimum mixture ratios of each amendment were 3% in A and B, and 7~10% in C. To analyze the corelation of mixture ratio versus to physical character, volume of porosity was significantly related to the ratio of B (P<0.05), and showed similar corelation in porosity and hydraulic conductivity with ratio of C(P<0.05). These results indicate that types and mixture ratio of inorganic soil amendments should affect on soil physio-chemical properties of root zone on USGA sand green.

• Journal of the Korean Institute of Landscape Architecture
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• v.49 no.2
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• pp.113-127
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• 2021

Although planning techniques linking parks, green areas, and waterways have become common, there are frequent disruptions in the operation and management of landscape-use artificial channels (LuAC). Therefore, this study examined a design to promote the sustainable management and operation of a LuAC using rainwater for the streamlets of the Jeonju-Wanju Innovative City. In order to accomplish the purpose of this study, scenarios were set up by dividing the design into waterhead and waterway portions. First, the scenario regarding the waterhead was analyzed to calculate the water supply and storage required for the waterway and waterhead. The analysis showed that the waterway requires a water supply of 676.8 tons/months, 3,018 tons to 5,512 tons of storage space, and a water depth of 0.75 m to 1.37 m considering the ecological and landscape aspects. The second scenario is to select an effective system of facilities for the operation and management of the LuAC. To accomplish this, a single-circulation system (SCS), which transports water to a highland location was compared to a multi-circulation system (MCS), which supplied water separately to each water space and operated independently. The results showed that the MCS, which was operated independently by small power units, was more effective owing to the vast difference in water supply operation times.

• Journal of Climate Change Research
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• v.2 no.2
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• pp.115-131
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• 2011

Out of all the possible actions that can be taken to respond to greenhouse gas reduction, including development of greenhouse gas reduction technology, infrastructure, actions to improve energy saving and efficiency, and offset with carbon emission reductions (CERs), this study shall focus on the investment on CERs. This study will take a look at risks involved with investing in CERs such as UN registration refusal risk and CERs price fluctuation, and will design risk management model which shall be verified. The goal of this paper is to provide optimized CERs investment strategies for different types of investors, such as general trading companies seeking for investment opportunities and financial companies with plans for green products development and investment by preparation for carbon market. It is expected that the global competitiveness of domestic financial companies shall be improved by taking actions on carbon market instead of previous passive response to climate change and that Korea, the number two Carbon Emissions supplier and number one derivatives market in terms of volume, shall be able to lead the worldwide carbon market.

• Journal of Climate Change Research
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• v.3 no.2
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• pp.153-159
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• 2012

CFC-12 used in mobile air conditioning(MAC) system has been replaced by R-134a, a type of HFC refrigerant, from 1991 to 1994. R-134a has since been widely used as a refrigerant of a mobile air conditioner. However, it is one of the six main green house gases listed in Kyoto Protocol, which makes it imperative to regulate its emission and develop alternative refrigerants. In this study, the concentration of leaked R-134a was measured using VT(Variable Temperature) shed and Running loss test shed to analyze the level of air conditioner refrigerant leaked in a vehicle. According to the analysis of the concentration of R-134a leaked from a vehicle parked, annual leakage amount of R-134a was in the range of 6.46~13.28 g/yr. The figure was similar with the leakage from the mobile air conditioning system currently used. In a study using the same vehicle model, a vehicle equipped with dual evaporation system had a higher leakage rate of refrigerant than a vehicle with a single evaporation system. It appears that the added fittings and joints of the dual evaporator system led to higher leakage rate. Besides, the analysis of the change in R-134a concentration under various car speed found that more refrigerant leaked under high speed(100km/hr) and but the volume of the wind did not affect to the variation of refrigerant leakage.