• Journal of Microbiology and Biotechnology
• /
• v.10 no.1
• /
• pp.115-118
• /
• 2000

Recombinant plasmids harboring a heterologous gene coding for the enhanced green fluorescent protein (EGFP) were transfected and expressed in Drosophila melanogaster S2 cells. A stable transformation of polyclonal cell populations expressing EGFP were isolated after 4 weeks of selection with hygromycin B. The recombinant EFGP expressed in transformed S2 cells consisted of a molecular weight of 27 kDa. EGFP expression was also confirmed by fluorometric measurement. The maximum EGFP concentration was about 9.3 mg/I. The present findings demonstrate not only the successful stable expression of EGFP in Drosophuila was about 9.3 mgI. The present findings demonstrate not only the successful stable expression of EGFP in Drosophila S2 cells, but also the use of EGFP as a reporter to analyze gene expression, with its potential of a Drosophila cell expression system for recombinant protein production being an alternative to a baculovirus-insect cell expression system.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.18 no.1
• /
• pp.143-149
• /
• 1993

An approach is presented for efficient numerical integration of the Sormnerfeld type integrals pertinent to the microstrip surface Green's function arising in the problem of an electric current point source on an infinite planar grounded dielectric substrate. This approach, valid for both lossless and lossy dielectric substrates, is based on the deformation of the integration contour via a coordinate transformation and Cauchy's residue theory, and identifies clearly the effects of surface waves. I ts useful application is in a rigorous moment method analysis of micros trip antenna arrays and microstrip guided wave structures. The efficiency and the usefulness of the present approach are emphasized through some numerical calculations of the impedance matrix elements with associated CPU times.

• International Journal of Advanced Culture Technology
• /
• v.8 no.4
• /
• pp.101-109
• /
• 2020

Along with rapid global economic development, people are slowly becoming environmentally aware and green development is increasingly a vital topic of social concern. Academic circles are currently focusing on environmental protection and economic growth to explore the coordinated development of both and to achieve a win-win solution between them. To study the relationship between environmental protection experience and both cost and performance, this study empirically analyzes companies listing on the Shenzhen A-share market from 2014 to 2018. The results show that senior executives' environmental protection experience is negatively correlated with corporate costs, whereas it is positively correlated with business performance. These results provide empirical evidence of the importance of enterprises introducing and cultivating environmental personnel and insisting on sustainable development.

• Journal of Plant Biotechnology
• /
• v.7 no.4
• /
• pp.211-218
• /
• 2005

Genetic transformation was carried out by using biolistic gun method. The hypocotyl derived embryogenic calli (explants) of cotton (Gossypium hirsutum L.) cv. Cocker-312 were transformed with a recombinant pGreen II plasmid, in which both, bar (selection marker) and GUS (${\beta}$-glucuronidase) reporter genes were incorporated. Explants were arranged on osmoticum-containing medium (0.5M mannitol) 4 hours prior to and 16 hours after bombardment that was resulted into an increase about >80% for GUS stable expression. 3 days after bombardment, GUS assay was performed, which exhibited, $18.36{\pm}1.00$ calli showed blue spots. The transformed embryogenic calli were cultured on selection medium (@ 6 mg/L basta) for 3 months. The putative transgenic plants were developed via selective somatic embryogenesis (@1.50 mg/L basta); maximum $27.58{\pm}1.25$ somatic embryos were obtained while $17.47{\pm}1.00$ embryos developed into plantlets (@ 0.75mg/L basta). In five independent experiments, up to 7.24% transformation efficiency was recorded. The presence of the transgenes was analyzed by using PCR and southern hybridization analysis. The transgenic plants were developed with in 6-7 months, but mostly transformants were abnormal in morphology.

• Fashion & Textile Research Journal
• /
• v.9 no.2
• /
• pp.138-144
• /
• 2007

The purpose of this research was to compare the harmony of tone on tone coloration according to transformation of color area-ratio in shirt and necktie. The respondents were asked to evaluate 48 stimuli of the shirt and necktie with the coloration of the shirt/necktie, the necktie/shirt was separately compounded to be 4 tones such as vivid, light, dull, dark. The subjects were 288 female undergraduate students. The data was analyzed by using SPSS program. Analysis methods were ANOVA, LSD test and t-test. The results of each can be summerized as follows. As to the red, combination of v-lt made a difference in the harmony of coloration according to the transformation of color area-ratio. As to the blue, combination of v-lt, v-dk, lt-d made a difference in the harmony of coloration according to the transformation of color area-ratio. As to the purple, v-lt, v-d was evaluated as harmonized coloration regardless of area-ratio by both male and female students. As to the green, v-lt, lt-d was evaluated as harmonized coloration regardless of area-ratio by both male and female students.

• Journal of Microbiology
• /
• v.43 no.4
• /
• pp.361-365
• /
• 2005

In this study, we chronicle the establishment of a novel transformation system for the unicellular marine green alga, Dunaliella salina. We introduced the CaMV35S promoter-GUS construct into D. salina with a PDS1000/He micro-particle bombardment system. Forty eight h after transformation, via histochemical staining, we observed the transient expression of GUS in D. salina cells which had been bombarded under rupture-disc pressures of 450 psi and 900 psi. We observed no GUS activity in either the negative or the blank controls. Our findings indicated that the micro-particle bombardment method constituted a feasible approach to the genetic transformation of D. salina. We also conducted tests of the cells' sensitivity to seven antibiotics and one herbicide, and our results suggested that 20 ${\mu}g$/ ml of Basta could inhibit cell growth completely. The bar gene, which encodes for phosphinothricin acetyltransferase and confers herbicide tolerance, was introduced into the cells via the above established method. The results of PCR and PCR-Southern blot analyses indicated that the gene was successfully integrated into the genome of the transformants.

• Applied Biological Chemistry
• /
• v.27
• /
• pp.56-67
• /
• 1984

As in many other country, the use of organic matter in Korea has long history. Farmers understand the value of organic matter as the source of plant nutrient and soil improving agent in general. Since 50 years ago, the sources of organic matter in paddy soils were compost, rice and barly straw, green manure, animal waste, fish and beancake, etc.. Application of green manures such as vetch and chinese milk vetch showed no significant effect on the yield of brown rice in paddy soil. On the other hand, the effects of compost and rice straw showed more significant on the yield of brown rice in paddy soil. Application of rice straw in rice cultivation is commonly made at different times between harvest, early spring and several weeks before transplanting. Considering the suitable paddy soil for application of rice straw under well to moderately well drained soil, the yield was pronounced more than poorly drained soil. Based on laboratory and field experimants, application of rice straw promoted the decrease of oxidation-reduction potential in well to moderately well drained soil. This results to be enhanced the release of some mineral nutrients,. such as potassium, calcium, silicon, and increase of availability of soil phosphorus. In the field experiments, results obtained from nitrogen fraction on the immobilization-mineralization of the tracer nitrogen applied in paddy soil,the amount and index of organic nitrogen incoporated in soil was more pronounced in rice straw application than control. Rice straw and its transformation products incoporated in the soil, provided the inflow of energy necessary to maintain heterotrophic microbes activities. Rice straw and its transformation products, especially soluble carbohydrate, enhanced the population of free-living heterotrophic $N_2$ - fixing microbes. Moreover, rice straw and its transformation products in paddy soil, enhanced the activities of soil enzymes such as dehydrogenase and urease.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.35 no.6
• /
• pp.603-608
• /
• 2011

Since PTV (particle tracking velocimetry) provides velocity vectors by tracking each particle in a fluid flow, it has significant benefits when used for nano- and bio-fluid flows. However, PTV has only been used for limited flow fields because interpolation data loss is inevitable in PTV in principle. In this paper, a hybrid particle image velocimetry (PIV) algorithm that eliminates interpolation data loss was constructed by using an affine transformation. For the evaluation of the performance of the constructed hybrid PIV algorithm, an artificial image test was performed using Green-Taylor vortex data. The constructed algorithm was tested on experimental images of the wake flow (Re = 5,300) of a rectangular body ($6cm\;{\times}3cm$), and was demonstrated to provide excellent results.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.7 no.2
• /
• pp.85-92
• /
• 1989

The aim of this study is to find out the combination of effective transformed data, applying Remote Sensing techniques, as to the classification and particular objects by transforming the MSS data and TM data of the satellite LANDSAT into several linearly transformed data. Since one of the problems in the processing of the LANDSAT data is the vastness of the data, the Linear Transformation could be a method to perform analysis of those vast data, more efficiently and economically. This method is carried out as follows : (1) offering the simplicity over complex data, (2) selectional processing over redundant data and removing unnecessary data, (3) emphasizing on the object of the study ; by transforming multispectral data through linear calculation and statistical transformation. In this study, the analysis and transformation of the data have been performed by means of Band Ratioing and Principal Component Analysis. As the classificatory consequence, Infrared/RED Ratioing which expands the characterization of green vegetation, has been useful for a distinctive classification among other classes. For the Principal Component Analysis, band 1,2,7 are efficient in the classification of the green vegetation.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.43 no.5
• /
• pp.675-687
• /
• 2023

The border area between the South and North Korea is considered a stage for promoting Green Détent through the transformation of the Demilitarized Zone into the Green Peace Zone. This paper proposes 'Prosuming-index' composed of items to evaluate the multi-layered nature of industrial and infrastructure projects in the ecological and environmental fields planned in the border area. Based on the prosuming-index, we derived the following four types of potential for Green Detent practice in the border area-first, the Incheon and Ganghwa regions as 'a logistics/human network and agricultural/fishery production area', second, the northern Gyeonggi region as a 'cooperative network area based on the smart technology industries', third, the western Gangwon and the parts of nothern Gyeonggi region, as 'a multiple prosumer's area based on carbon neutral technologies', lastly, the eastern Gangwon region, as 'a transition area from idle or aged infrastructure to green infrastructure through the renewable energy industries'.