• ALGAE
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• 제25권2호
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• pp.65-70
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• 2010

The genus Chaetoceros provides highly diversified diatoms in marine systems. Morphological descriptions of the genus are well-documented, yet the DNA taxonomy of Chaetoceros has not been satisfactorily established. Here, the molecular divergences of the 18S-28S rDNA of Chaetoceros were assessed. DNA similarities were relatively low in both 18S (93.1 $\pm$ 3.9%) and 28S rDNA (81.0 $\pm$ 4.6%). Phylogenies of the 18S, 28S rDNAs showed that Chaetoceros was divided according to individual species, clustering the same species into single clades. Statistical analysis with corrected genetic (p-) distance scores showed that nucleotide divergence of Chaetoceros 28S rDNA significantly differed from that of 18S rDNA (Student's t-test, p < 0.05). This finding suggests that the 28S rDNA may be treated as a more suitable marker for species-level taxonomic distinctions of Chaetoceros.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.105-109
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• 2004

This study was conducted to investigate the potential origin of Weissella species, which were found in ingredients of kimchi, such as salted Chinese cabbage, radish, green onion, red pepper powder, pickled shrimps, garlic, and ginger. Ten strains of Weissella species (Weissella thailandensis, W. kimchii, W. koreensis, W. minor, W. halotolerans, W. hellenica, W. kandleri, W. confusa, W. viridescens, and W. paramesenteroides) and lactic acid bacteria isolated from ingredients of kimchi were analyzed by SDS-PAGE of whole-cell proteins. Several strains with patterns identical to those of Weissella kimchii were isolated from green onion. On the basis of biochemical characteristics and 16S rDNA sequence comparisons, these strains were identified as Weissella kimchii, suggesting green onion as a major origin of Weissella kimchii found in kimchi.

• 생명과학회지
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• 제18권10호
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• pp.1331-1335
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• 2008

여러 가지 난분해성 색소에 대하여 탈색능을 나타내는 Klebsiella pneumoniae WL-5이 염색폐수처리장의 활성슬러지로부터 분리되었다. 이 세균은 정치배양과 at pH 6-8 및 $30-35^{\circ}C$에서 높은 탈색능을 나타내었다. Congo Red색소에 대해서는 $200\;{\mu}M$ 농도에서 12시간 배양하였을 때 90% 이상이 탈색되었고, Malachite Green, Brilliant Green, Reactive Black-5에 대해서는 $10\;{\mu}M$ 농도에서 80% 이상이 탈색되었지만, Reactive Red-120, Reactive Orange-16, Crystal Violet에 대해서는 $10\;{\mu}M$ 농도에서 각각 46%, 25%, 13%의 비교적 낮은 탈색능을 나타내었다. 트리페닐메탄계 색소는 세포표면에의 흡착에 의한 탈색을 나타내었고, 아조계 색소는 지금까지 알려져 있지 않는 새로운 효소반응계에 의해서 탈색된다는 것을 제시하였다.

• 농업과학연구
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• 제40권4호
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• pp.377-383
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• 2013

Premature-green rice is typically obtained by early harvest when culms of rice still appear green in color, and the rice and its food products have been considered as wellbeing foods. This study was carried out to determine the quality characteristics of cookies made from flour added with 10, 20, and 30% whole green rice powde r(WGRP) of two kinds of Hopum and Shinsun waxy rice. The quality characteristics of cookies, including pH, spread factor, color, hardness, and sensory properties, were estimated. WGRP with different levels of 10, 20, and 30% was added into powder for preparing cookies, and their quality properties were evaluated. The pH and hardness of the cookies increased, while spread factor showed highest added with 20% Hopum and Shinsun waxy rice. The color (L) of cookies decreased 20% added with Hopum and Shinsun waxy rice. The sensory properties of cookies was highest added with 20% Hopum and 10% Shinsun waxy rice. The result of this study suggest that addition of 20% Hopum and 10% Shinsun waxy rice are available rice cookies. It was concluded that WGRP may have a potential in bakery industry as a new food material.

• 인간식물환경학회지
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• 제24권6호
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• pp.639-649
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• 2021

Background and objective: Various images from visual display terminals (VDTs) as well as living lighting are important parts of our daily life; thus, properly controlling the lighting environment - that is, illuminance, color temperature and good images from VDTs - can have a substantial effect on improving the mental health and work efficiency in everyday life. We examined electroencephalography (EEG) and heart rate variability (HRV) responses to various lighting conditions in 25 university students as they viewed images of a green landscape or traffic congestion. Methods: EEG was performed in darkness and when the room was illuminated with 10 different light-emitting diode (LED) color temperatures, while the EEG and HRV responses to green landscape or traffic congestion image stimuli were measured in darkness and during room illumination with three different LED color temperatures. Results: We found a significant difference between darkness and high LED illumination (400 lx) at 7 (CZ, F4, FZ, O1, O2, OZ, and T6) of 30 channels, while the alpha wave activity increased during darkness. In the second experiment, the green landscape image stimuli in the 30 lx-2600 K lighting condition elicited theta wave activity on the EEG, whereas the traffic congestion image stimuli under high LED illumination elicited high beta and gamma wave activities. Moreover, the subjects exhibited better stress coping ability and heart rate stability in response to green landscape image stimuli under illuminated conditions, according to their HRV. Conclusion: These results suggest that lower color temperatures and illumination levels alleviate tension, and that viewing green landscape image stimuli at low illumination, or in darkness, is effective for reducing stress. Conversely, high illumination levels and color temperatures are likely to increase tension and stress in response to traffic congestion image stimuli.

• 한국조경학회지
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• 제31권4호
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• pp.1-12
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• 2003

The urban park provides a safe rest and leisure area, and offers the beauty of nature to counter the drearyness of urban space. It can provide benefits such as the reduction of environmental pollution, the regulation of the local climate, and it can also provide a safe area during times of disaster. However, there was no interest in urban park development and control in Korea during the economic boom of the mid 1960s. Furthermore, during the industrialization process, the population grew significantly in the cities and the cities' scales were noticeably extended; as a result of this, the living environment and the natural environment in the cities worsened. With rapid urbanization and industrialization, urban green area diminished, and it became necessary to organize the urban park system to improve quality of life. 45% of south korea's population lives in 6 cities, which is only 4% of Korea's land size. The Urban park system has to be considered in the urban planning process. Paris' urban green system can be a role-model for Korea's urban green development plan to function organically. Urban public park concepts have been used in Paris's urban planning since 1850. There were hardly any parks, gardens and squares for the public before the middle of the 19th century. For improving life-styles for the poor, Napoleon III strongly supported the development of green space systems in Paris by G.E. Haussmann. Napoleon III and Haussmann established and applied the urban green regulation within Paris urban planning. The purpose of this study is to investigate the green regulations Haussmann's Paris urban plan and urban green space system: and as a result of this, it can be an indicator for urban green space development in Korea.

• Food Science and Biotechnology
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• 제15권6호
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• pp.838-843
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• 2006

The chemical processes involved in the formation of green pigment in crushed garlic cloves were investigated based on the principle of pink pigmentation in macerated onions. Intact greening and non-greening garlic cloves were either left untreated or heated at $90^{\circ}C$ for 3 min to inactivate enzyme activities. First, a colorless ether soluble compound referred to as color developer reacted with glycine (among all free amino acids) in garlic to form a second compound insoluble in ether. The latter compound then reacted with formaldehyde to yield the green colored pigment. Alliinase activity was necessary for the production of color developer and for the development of green pigment. In greening garlic that had been heat treated, green pigmentation did not proceed due to the heat-inactivation of alliinase, but the addition of alliinase solution into the garlic homogenates restored the pigmentation. However, this phenomenon was not observed in non-greening garlic with or without heat treatment. Finally, the mechanism of green pigment formation in crushed garlicis similar to that of pink pigment formation in macerated onions.

• 동아시아식생활학회지
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• 제15권1호
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• pp.57-64
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• 2005

The effects of water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower on serum lipid concentrations were evaluated in rats. Forty-eight male Sprague-Dawley rats weighing l00±l0 g were divided into six groups and fed high fat diets for six weeks. Experimental groups were administered with following diets; Control diet, animal, plant high fat diet and control and high fat diets with 2% water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower. Tissue weights of liver, lung, stomach, heart, kidney and spleen of high fat diet exposed rats were reduced by water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups. The concentrations of serum triglyceride in rats fed the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower were lower than those in other groups. The concentrations of total cholesterol in water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower group were lower than those in high fat diet groups. The concentrations of HDL-cholesterol in serum of the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups were tended to be lower than those of other groups. GPT and GOT activities were decreased in water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups and than in the high fat group. LDH activity was lower in the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups than in the high fat group. These results suggest that water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

• 동아시아식생활학회지
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• 제16권4호
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• pp.447-452
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• 2006

This study examined the growth and sensory characteristics of soybean sprout cultured at 25$\pm$1$^{\circ}C$ for 4 days with distilled water control and green tea extracted water (0.03% and 0.05%). The proximate composition of soybean sprout in the green-tea water was better than that of the control in ash, protein and fat, while the soybean sprout was especially higher in 0.05% green-tea water. The contents of vitamin C and $\beta$-carotene were higher in soybean sprout in green-tea water than the control. The total free amino acids composition of soybean sprout in green-tea water was better than that of the control, with the highest being obtained in soybean sprout in 0.03% green-tea water. Antioxidative activity was assayed by DPPH radical scavenging ability with spectrophotometer at 514 nm. The soybean sprout in green-tea water was higher than control. The amylase activity of the soybean sprout increased steadily during the first 4 days and that of the control was higher than soybean sprout in green-tea water. The proteinase of soybean sprout steadily increased during 4 day culture. Furthermore, the proteinase activity of soybean sprout in green-tea water was higher than that of the control up to 2 days. Whereas after 3 days, it was the highest in 0.03% green-tea water and then decreased remarkably in 0.05% green-tea water.

• 생명과학회지
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• 제14권1호
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• pp.21-25
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• 2004

Crystal violet와 malachite green색소 분해에 관여하는 유전자들을 규명하기 위하여 색소분해능을 가진 Citrobacter sp.의 염색체 DNA속에의 transposon 도입에 의해 생성된 무작위 변이주들이 분리되었다. 이들 변이 주들로부터 두가지 색소분해능을 소실한 14개의 변이주들이 선별되었고, 이들로부터 염색체 DNA를 분리하여 EcoRl으로 절단한 후 Tn5 단편을 probe로하여 Southern hybridization을 행한 결과, 염색체 DNA상의 각각 다른 부위에 Transposon이 Single 삽입된 5개의 변이주 (Cmg2, Cmg6, Cmg8, Cmgll, Cmg12)가 최종적으로 분리되었다. 이들 변이주들의 Transposon 삽입부위 주위의 염기서열과 이로부터 유추되는 아미노산서열을 database상에 등록되어 있는 유전자의 염기서열과 단백질의 아미노산 서열에 대한 상동성을 비교한 결과, Cmg2는 대장균 maltose trnasporter (Mal C)이고, Cmg6은 LysR-type 전사조절 단백질이며, Cmg12는 산화환원효소를 코드하는 유전자인 것으로 알려졌고, 나머지 Cmg8과 Cmg11은 아직까지 기능이 알려져 있지 않은 단백질을 코드하는 유전자인 것으로임이 판명되었다.