• The Journal of the Convergence on Culture Technology
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• v.5 no.4
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• pp.467-472
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• 2019

Seaweeds are rich in in minerals, vitamins, proteins, and fiber, and are classified as green algae, and they are distributed on the coasts of Korea, East Asia, Oceania, etc. and are used as a health function material as well as food ingredients in our countries and countries. In this study, Codium fragile was extracted from ethanol and concentrated to confirm DPPH radical scavenging activity, SOD activity, FRAP and ABTS cation radical scavenging ability. As a result, DPPH scavenging activity was 0.83, 22.83, 38.27, 40.93, 45.60% at 6.25, 12.50, 25, 50 and $100{\mu}g/mL$, SOD-like activity were 23.13, 33.63, 33.93, 44.07 and 59.07%. FRAP and ABTS showed antioxidant activity in a concentration-dependent manner, as in the previous experiment. Therefore, this study confirmed that it can be used as a cosmetic material using Codium fragile, a natural material.

• Journal of the Society of Naval Architects of Korea
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• v.38 no.3
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• pp.41-46
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• 2001

The very large vessels like VLCC and container ship have been built recently and those vessels have smaller structural strength in comparison with the other convectional skips. As a result the fatigue destruction of upper deck occurs a frequently due to the springing phenomenon at the encountering frequencies. In this study, the hydrodynamic loads are calculated by three-dimensional source distribution method with the translating and pulsating Green function. A ship is longitudinally divided into 23 sections and the added mass, damping and hydrodynamic force of each section is calculated. focusing only on the vertical motion. Stiffness matrix is calculated by the Euler beam theory. The calculation is carried out for Esso Osaka.

• Geomechanics and Engineering
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• v.19 no.1
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• pp.11-20
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• 2019

The use of electrokinetic dissipation method to study the fluid flow law in micro-pores is of great significance to reservoir rock microfluidics. In this paper, the micro-capillary theory was combined with the coupling model of the seepage field and the current field under the excitation of the harmonic signal, and the coupling theory of the electrokinetic effect under the first-order approximation condition was derived. The dissipation equation of electrokinetic dissipation and viscous resistance dissipation and its solution were established by using Green's function method. The physical and mathematical models for the electrokinetic dissipation of reservoir rocks were constructed. The microscopic mechanism of the electrokinetic dissipation of reservoir rock were theoretically clarified. The influencing factors of the electrokinetic dissipation frequency of the reservoir rock were analyzed quantitatively. The results show that the electrokinetic effect transforms the fluid flow profile in the pores of the reservoir from parabolic to wavy; under low-frequency conditions, the apparent viscosity coefficient is greater that one and is basically unchanged. The apparent viscosity coefficient gradually approaches 1 as the frequency increases further. The viscous resistance dissipation is two orders of magnitude higher than the electrokinetic effect dissipation. When the concentration of the electrolyte exceeds 0.1mol/L, the electrokinetic dissipation can be neglected, while for the electrolyte solution (<$10^{-2}M$) in low concentration, the electrokinetic dissipation is very significant and cannot be ignored.

• The Plant Pathology Journal
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• v.37 no.2
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• pp.182-193
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• 2021

The transcription factor SHE1 was identified as an interacting partner with the cucumber mosaic virus (CMV) 1a protein in the yeast two-hybrid system, by a pull-down assay, and via bimolecular fluorescent complementation. Using fluorescent-tagged proteins and confocal microscopy, the CMV 1a protein itself was found distributed predominantly between the nucleus and the tonoplast membrane, although it was also found in speckles in the cytoplasm. The SHE1 protein was localized in the nucleus, but in the presence of the CMV 1a protein was partitioned between the nucleus and the tonoplast membrane. SHE1 expression was induced by infection of tobacco with four tested viruses: CMV, tobacco mosaic virus, potato virus X and potato virus Y. Transgenic tobacco expressing the CMV 1a protein showed constitutive expression of SHE1, indicating that the CMV 1a protein may be responsible for its induction. However, previously, such plants also were shown to have less resistance to local and systemic movement of tobacco mosaic virus (TMV) expressing the green fluorescent protein, suggesting that the CMV 1a protein may act to prevent the function of the SHE1 protein. SHE1 is a member of the AP2/ERF class of transcription factors and is conserved in sequence in several Nicotiana species, although two clades of SHE1 could be discerned, including both different Nicotiana species and cultivars of tobacco, varying by the presence of particular insertions or deletions.

• Journal of the Korea Society of Computer and Information
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• v.26 no.8
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• pp.181-185
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• 2021

Road fine dust is not simply a problem of air pollution, but is threatening the health and life of the public, including pedestrians using sidewalks. There are various policy proposals related to road fine dust reduction, but the actual effect cannot be guaranteed. Therefore, in order to ensure the safe and pleasant passage of the people, the pavement pavement (sidewalk pavement) for fine dust management on the road should be designed by considering the location, use, function, etc., and appropriate paving materials and construction methods should be selected and designed or sidewalk facilities should be prepared. Therefore, this study examines the concept and legal basis of sidewalk pavement, reviews the status of sidewalk pavement-related technologies that purify air pollution, and utilizes sidewalk pavement materials under the revised sidewalk pavement guidelines for reducing fine dust, 「Act on Promotion of Purchasing of Green Products」 We intend to establish a legal basis for early application of sidewalk pavement technology by proposing measures and amendments to local government sidewalk pavement management rules.

• Journal of the Semiconductor & Display Technology
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• v.20 no.4
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• pp.89-95
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• 2021

As the influence of fine dust on society spreads gradually, the public's interest in indoor air is increasingly rising. Air-purifying plants are drawing keen attention due to their natural purifying function enabled by plant physiology. However, as their fine dust reduction mechanism is limited to adsorption only, vegetation bio-filters that optimize purification effects through integration with air-conditioning systems is rising as an alternative. In accordance with the relevant standard test methods, this study looked into the fine dust reduction assessment method by air-conditioning airflow volume that can be used for the industrial spread of vegetation bio-filters. In the case of PM₁₀ at 300 ㎍/m³, it was in the order of EG-B(3,500CMH, 29 min.) < EG-A (2,500CMH, 37 min.) < CG(0CMH, 64 min.) for reaching the maintenance level (100 ㎍/m³) of publicly used facilities. For reaching the WHO Guideline(50 ㎍/m³) requirement, it was in the order of EG-B (51 min.) < EG-A (160 min.) < CG (170 min.). In the case of PM_2.5, it was in the order of EG-B (26 min.) < EG-A (33 min.) < CG (57 min.) for reaching the maintenance level (50 ㎍/m³) of publicly used facilities. It was in the order of EG-B (48 min) < EG-A (140 min) < CG (158 min) for reaching the WHO Guideline (25 ㎍/m³) requirement. The findings from the analysis showed that fine dust can be reduced most efficiently when the system is operated at 3,500CMH level. The limitation of this study is that due to the absence of a way of assessing the stress of plants in vegetation bio-filters, generating optimal air-conditioning air flow of the relevant system and economics analysis against the existing facility-type air purification system have been clarified, which should be explored further though follow-up studies.

• Journal of the Korean Society of Industry Convergence
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• v.25 no.4_2
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• pp.587-594
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• 2022

In the 2000s, a lot of cable-type grand bridges are being built in consideration of economic aspects such as the reduction of logistics costs and the distribution of traffic volume due to rapid economic development. In addition, because the recently installed grand bridges are designed in an aesthetic form that matches the surrounding environment as well as the original function of the road bridge, and serves as a milestone in an area and is used as an excellent tourism resource, attracting many vehicles and people, there is an urgent need for a safety structure that can ensure the safety of not only vehicles but also people. In order to make cable-stayed bridge safe on wind for additional five safety structures, main girder models with and without safety structures for wind-tunnel experiments was made, and wind tunnel experiments was carried out to measure aerodynamic force coefficients. Also, wind-resistant analyses of 3D cable-stayed bridge were performed on the basis of wind-tunnel experiment results. From the wind tunnel experiments for the aerodynamic force coefficients of main girder with five safety structures and the wind resistant analyses of cable-stayed bridge without safety structure and with safety structure, it was concluded that the best form of wind-resistant safety was shown in the order of mesh, standard, bracing, hollow, and closed type. And wind-resistant safety of cable-stayed bridge with hollow and closed type on design wind speed 68.0m/sec was not secured. Finally, as five safety structures are installed, maximum rate of stress increments was shown in the order of steel main beam, steel floor beam, concrete floor beam and cables.

• Advances in nano research
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• v.15 no.3
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• pp.263-275
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• 2023

The electron transport properties of Y-type zigzag branched carbon nanotubes (CNTs) are of great significance for micro and nano carbon-based electronic devices and their interconnection. Based on the semi-empirical method combining tight-binding density functional theory and non-equilibrium Green's function, the electron transport properties between the branches of Y-type zigzag branched CNT are studied. The results show that the drain-source current of semiconducting Y-type zigzag branched CNT (8, 0)-(4, 0)-(4, 0) is cut-off and not affected by the gate voltage in a bias voltage range [-0.5 V, 0.5 V]. The current presents a nonlinear change in a bias voltage range [-1.5 V, -0.5 V] and [0.5 V, 1.5 V]. The tangent slope of the current-voltage curve can be changed by the gate voltage to realize the regulation of the current. The regulation effect under negative bias voltage is more significant. For the larger diameter semiconducting Y-type zigzag branched CNT (10, 0)-(5, 0)-(5, 0), only the value of drain-source current increases due to the larger diameter. For metallic Y-type zigzag branched CNT (12, 0)-(6, 0)-(6, 0), the drain-source current presents a linear change in a bias voltage range [-1.5 V, 1.5 V] and is symmetrical about (0, 0). The slope of current-voltage line can be changed by the gate voltage to realize the regulation of the current. For three kinds of Y-type zigzag branched CNT with different diameters and different conductivity, the current-voltage curve trend changes from decline to rise when the branch of drain-source is exchanged. The current regulation effect of semiconducting Y-type zigzag branched CNT under negative bias voltage is also more significant.

• Journal of Microbiology and Biotechnology
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• v.34 no.6
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• pp.1222-1228
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• 2024

Protein-specific antibodies are essential for various aspects of protein research, including detection, purification, and characterization. When specific antibodies are unavailable, protein tagging is a useful alternative. Small epitope tags, typically less than 10 amino acids, are widely used in protein research due to the simple modification through PCR and reduced impact on the target protein's function compared to larger tags. The 2B8 epitope tag (RDPLPFFPP), reported by us in a previous study, has high specificity and sensitivity to the corresponding antibody. However, when attached to the C-terminus of the target protein in immunoprecipitation experiments, we observed a decrease in detection signal with reduced immunity and low protein recovery. This phenomenon was not unique to 2B8 and was also observed with the commercially available Myc tag. Our study revealed that C-terminal tagging of small epitope tags requires the addition of more than one extra amino acid to enhance (restore) antibody immunities. Moreover, among the amino acids we tested, serine was the best for the 2B8 tag. Our findings demonstrated that the interaction between a small epitope and a corresponding paratope of an antibody requires an extra amino acid at the C-terminus of the epitope. This result is important for researchers planning studies on target proteins using small epitope tags.

• Journal of the Korean Society of Industry Convergence
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• v.27 no.4_2
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• pp.833-840
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• 2024

According to recent data from the Korea Meteorological Administration(KMA), the frequency of typhoons around the Korea Peninsula is almost unchanged, but the intensity is on the rise due to climate change. A typhoon that has become so powerful can cause partial or complete damage to the traffic signal structures, limiting the operation of the vehicle and causing traffic congestion. If the traffic signal structure fails to function properly due to the influence of the typhoon, not only the v ehicle operation will be disrupted, but also direct damage to the traffic signal structure will occur. In addition, if the social overhead cost of traffic congestion is included, the recovery cost caused by the typhoon will increase to an extent that it is difficult to estimate. Therefore, in this study, a wind tunnel experiment was performed by producing a wind tunnel model of an existing fixed traffic signal structure and a traffic signal structure in which signs and traffic lights are hinged. Also, The fixed and hinge structures were modeled as 3D finite elements, and wind-resistant analysis was performed by wind speed, and, wind-resistant safety of traffic signal structures were analyzed and examined through wind-resistant analyses. From the comparative analysis of the results of experiment and FE analysis, it was known that the stress reduction rate of the hinge connection structure was at least 30% compared to that of the fixed connection structure from the results of the wind tunnel experiment and FE analysis. And As a result of finite element analysis for the maximum design wind speed of 50m/s, it was found that the maximum stress generated in the existing structure exceeded all the yield stress, but the maximum stress of the hinge connection structure was within the yield stress. Finally The hinge connection structure showed a relatively large stress reduction rate as the wind speed increased and the length of the lateral beam was shorter at the same wind speed.