• Food Science of Animal Resources
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• v.23 no.3
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• pp.262-268
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• 2003

Kefir is a traditional fermented milk in Caucasusian area and is made mainly of milk fermented with lactic acid bacteria and yeasts. Six typical kefir grains were selected from ten kefir grains collected from different locals in Korea. Kefir grains were gelatinous in texture and had various shapes of villi, grapes, leaves, hulled millets, and towels. To investigate predominant microflora of kefir grains, SPC, MRS, M17, Rogosa, and APT agar media were used for viable cell count MRS, SPC, and Rogosa media were most acceptable for bacterial cell counts of the selected kefir grains. From one or two of the SPC agar plates which contained around 25∼50 colonies, all grown colonies were isolated and identified. Most predominant bacteria was identified as Lactobacillus fermentum by API 50 CHL kit. The proportions of Lb. fermentum and Lb. brevis among the total identified bacteria were around 41~88% and M4%, respectively. To select the best preservation method for kefir grains, refrigeration, freezing, and freeze drying were compared. Freeze drying was found most suitable for the preservation of kefir grains, based upon their acid-producing activities and production of off-flavors.

• Journal of Applied Biological Chemistry
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• v.61 no.3
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• pp.291-295
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• 2018

Metcalfa pruinosa is a pest causing widespread problems to many crops in Korea. This pest infects fruit crops especially, persimmon and grapes. We tested the possibility of M. pruinosa management using essential oils of Alpina galangal and methylcinnamate which were extracted from A. galangal by steam distillation method. The use of essential oil showed a mortality rate of 10.0 and 23.3% for adults and nymphs, respectively. While the use of methylcinnamate resulted to a mortality rate of 40.0% in adults and 36.6% in nymphs. For its repellent effect, it showed an avoidance rate of 50.0 and 63.3% for adult and nymph respectively. Considering these two results, the extract of A. galangal are shown to have some synergic effect for pest control. The result of this study showed a possibility of M. pruinosa control using essential oil and methylcinnamate from A. galangal.

• Korean journal of applied entomology
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• v.15 no.2 s.27
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• pp.95-100
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• 1976

A distribution status of host plants for fruit-piercing moths was surveyed in Kocksung fruit growing area of Chonnam province during June to October, 1975. The results were summarized as follows; Main host plants of fruit·piercing larvae Smilax china L., Allizzia zulibrissim Dura, Cocculus trilobus Dc., Thalictrum aquilegifolium L., and Pterocarya rhoifolia Sieb & Zucc. Pterocarya rhoifolia Sieb & Zucc. were found to be distributed at mountain regions over 500m above sea level, while the other host plants were found to be distributed with in loom to 300m above sea level The moths was found from June 25th to Oct. 4th. Maximum emergence of the moths on plum an8 peach were about July 18th and on the grapes tree about August 31th. The moths collected from June 25th to Oct.4th were 17 spcies. Dermaleipa zuno Dalman $(35.7\%)$, Oraesia emaginata Fabriciul$(17.7\%)$, Oraesia excavata Butler $(12.9\%)$, Adrias tyramus amurensis Staduinger$(9.1\%)$, were collected much more than any other species; They accounted for approximately$70\%$ of total moths collected. In regards to the sugar contents of peach and plum, it was observed that the more the sugar concentrated, the more the fruits damaged. but the acid value of fruits had not shown any significant relationship with their attractants.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.113-118
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• 2015

The objective of this experiment was to evaluate the effects of application wide plastic shelter (WPS) in the 'Campbell Early' grape. WPS was developed 30cm wider than standard plastic shelter (SPS; 210cm). Under the plastic shelter, the average daily temperature was $2{\sim}3^{\circ}C$ higher than ambient temperature, but WPS treatment was $0.5{\sim}1^{\circ}C$ higher than SPS treatment. However, relative humidity 15~20% lower than ambient and shown different result between two treatments annually. The occurrence of brown leaf spot disease caused by Pseudocecospora vitis on the vineyard decreased 55.6% (2012) and 17.2% (2013) under WPS than SPS. Grape bunches collected two harvest seasons (2012, 2013), were analyzed fruit quality (total soluble solid; TSS, titratable acidity; TA, berry weight, berry size). In 2012, grapes had no differences in the quality of two treatments, but in 2013, significantly increase $0.4^{\circ}Bx$ of TSS and decrease 0.8% of TA on the WPS than SPS. It suggests that the application WPS reduced the incidence of brown leaf spot disease annually, and then affect to the fruit quality.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.206-211
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• 2016

Modified T trellis (MT) and Wakeman trellis (WT) were widely used Korean vineyard because they had an advantage for spur pruning type cultivar such as Campbell Early. In this experiment, we compared labor time and intensity for bunch management between MT and WT trellis systems in 'Campbell Early' grapes. As a result, berry thinning was required 17.3 hours (10a) on the WT trellis but was required 12.3 hours (10a) on the MT trellis. In like manner, bagging was required 10.1 hours (10a) on the WT trellis but was required 8.2 hours (10a) on the MT trellis. On the other hand, labor intensity measured on berry thinning and bagging practices using REBA (Rapid Entire Body Assessment) index, then WT trellis was scored 13.0, but MT trellis was scored 8.6. Meanwhile, MT trellis reduced vigorous growing of internodes length and width on grapevine shoots. Consequently, MT trellis is more convenience trellis for working ergonomically in Korea vineyard.

• The Korean Journal of Community Living Science
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• v.22 no.3
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• pp.417-427
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• 2011

This research obtained fundamental data for the development of grape foods by investigating the preference for grape foods, popularization and commercialization plan of grape foods and obtained knowledge required in order to develop grape foods. The study used 354 consumers in Seoul and GyeongGi Province. The distribution of sex on research subjects was as follows. Male was 52.0% (184 persons) and female was 48.0% (170 persons). Age distribution varied by twenties and less than twenties being 30.8%, thirties being 26.3%, forties being 24.6% and the fifties and over being 18.4%. According to the results, preference for grape foods of subjects was that rice wrapped in grape leaves ($2.14{\pm}0.7$) and sweet steamed rice($2.12{\pm}0.8$) were the highest among staple food; grape vinegar($2.38{\pm}0.7$) and grape taffy($2.25{\pm}0.7$) were the highest among spices; grape jelly ($2.53{\pm}0.6$) and grape pudding($2.45{\pm}0.7$) were the highest among snacks; grape juice ($2.70{\pm}0.5$) and grape yogurt($2.59{\pm}0.6$) were the highest among beverages. Subjects responded to the fruit group among food groups harmonized with grape foods(p<0.01). 50.7% of subjects responded to "have to be delicious" and 25.1% of subjects responded to "nutritional balance" as the most important aspects of the development of grape foods. In the popularization and commercialization plan of grape foods, Subjects chose with respected importance, personal preference($4.15{\pm}0.8$), price($4.05{\pm}0.8$) and promotional strategy($4.00{\pm}0.9$). In conclusion, the food development and revitalization plan should use grapes to find out food materials suitable for grape mixture and nutritional balance. We will expect an increase population and commercialization of grape foods if we develop grape foods and promote strategically in consideration of the preference of consumers and the price of produce.

• Korean journal of applied entomology
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• v.13 no.4 s.21
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• pp.217-225
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• 1974

The damage by fruit-piercing moths in orchards, primarily on grapes cultivated on reclaimed hillsides in Koksung, Cholla Namdo, was surveyed during July to October, 1974. Moths collected at night, mostly with the aid of flash light, were then classified. Results are summairzed as follows; 1. In addition to the 14 species of fruit-piercing moths reported in Korea, 11 new species were collected lot the first time ia Korea. These include. Speiredonia retorta Clerck, Cocytodes coerulea Guenee, Metopta rectifasciata Menetries, Ophideres fullonica Linnaeus, Serrodes campana Guenee, Mythimna turca Linnaeus, Amphipyra livida Schiffermuller et Denis, Paralleia maturata Walker, Ophiusa tirhaca Cramer, Anomis mesogona Walker and Thyas dotata Fabricius. 2. The maximum emergence of moths in Koksung Orchard was from the 10th to the end of September. Only a few moths were observed befroe the begining of August or after October 10. 3. The number of moths flying to grape vines increased as the time advanced from sunset to midnight. Populations gradually decreased thereafter until 5:30 a. m., when only occasional moths were observed. 4. Average damage to grape fruits by these insects was virtually nil until August 10; with subsequent infestation rates of $1.0\%$ on August 15, $17.6\%$ on September 26, and $20.3%$ on October 4, respectively. 5. Fruits of less than 10 percent sugar content and higher than 6.2 acid value were free from damage by these moths, and those of higher sugar content and lower acid value to be more heavily attacked.

• Journal of the East Asian Society of Dietary Life
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• v.26 no.6
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• pp.559-564
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• 2016

We investigated the effect of calcium carbonate on the quality of wine obtained from Gaeryangmerou (Vitis. spp.), grapes, which are commonly used in wine making in Korea. Alcoholic fermentation was carried out at $25^{\circ}C$, for 7 days in the presence of 0.1%, 0.2%, and 0.3% calcium carbonate. As calcium carbonate concentration increased, the pH of wine increased, while its total acid content and redness decreased. Calcium carbonate treatment during precipitation and aging is more effective than during fermentation. Concentrations of alcohol, total anthocyanin, polyphenol, and tannin showed no significant differences between controls and deacidified groups. Tartaric and malic acids were found to be the major acids in Gaeryangmerou wine. Calcium carbonate reduced total acidity by precipitating tartaric acid. In the sensory evaluation of the acidity, and overall acceptability, wine treated with 0.1% calcium carbonate was the best. Higher calcium carbonate concentration, was associated with greater reduction in total wine acidity. However, it is necessary to maintain the calcium carbonate concentration within 0.1% since excessive amounts of calcium carbonate can have a negative effect on wine quality.

• Korean Journal of Agricultural Science
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• v.24 no.2
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• pp.132-137
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• 1997

Effective storage facilities are required to stabilize the price of agricultural and marine products to preserve their qualities due to the big fluctuation of shrimp price in Korea. It is easy to make the cave because of good conditions of the land configuration, soil and convenient transportation. The cave storage can save the cost about 40% in building site and equipment, and about 50% in maintenance comparing to existing low temperature storage facilities. The cave storage provide to improve the quality of their stored products with the low heat conductivity, the constant temperature and humidity year round. Therefore, more low temperature storage facilities are required because the items are expanded from potatoes, sweet potatoes, onions, garlics, apples and chestnuts to tangerines, grapes, cabbages, radishes, and wet ginsengs. The demands of the low temperature storage facilities can be substituted into the cave storage facilities. Thus, studies are conducted to observe the changes of the components of the pure and the seasoned salted shrimps with fermentation period during stored at room temperature in cave and to establish the storing at underground facilities to produce high quality salted fish to make profit.

• Korean Journal of Agricultural Science
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• v.47 no.3
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• pp.693-705
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• 2020

Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna^® Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.