• 제목/요약/키워드: Granulosa

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A Systematic Study on Octocorallia in Korea: 5. Paramuriceidae (Holaxonia: Gorgonacea

  • Song, Jun-Im
    • 한국동물학회지
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    • 제23권1호
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    • pp.25-40
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    • 1980

  • 著者는 한국산 八放珊瑚類의 계통분류학적 연구를 하기 위하여 1965년부터 1978년까지 제주도의 濟州, 爲美里, 林島, 西歸浦, 烏島, 門島, 虛島, 橫干島와 해운대의 尾浦등 9個 地域으로부터 채집한 標本들을 同定, 分類한 결과 다음과 같은 측뾰족산호科에 속하는 7屬 14種의 韓國未記錄種이 밝혀졌다. Bebryce indica, B. thomsoni, B. brocki, Calicogorgia granulosa, Filigella mitsukuri, Muricella abnormalis, Paracis pustulata, P. ijimai, Plexauroides reticulata, P. praelonga, P. rigida, P. complexa, Villogorgia alterans 및 V. antillarum.

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염색체 분석 기법에 의한 소 체외수정란의 성 조절 (Sexing by the Chromosome Analysis of In Vitro Fertilized Embryos in Cattle)

  • 손시환;박충생;송상현
    • 한국가축번식학회지
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    • 제20권2호
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    • pp.179-190
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    • 1996

  • Sexing and developing from splitted embryos which were fertilized in vitro implicate a possibility of production of the superior and sex controlled individuals. This study was carried out to investigate the production of transferable late blastocysts from in vitro fertilized embryos and to analyze sex by chromosome analysis from same embryos. In results, the ratio of cleavage and fertility of bovine follicular oocytes matured in vitro was 90% in co-cultured with granulosa cells. The competence of embryonic development from in vitro matured and fertilized bovine oocytes was 38% in co-cultured with bovine oviductal epithelial cells. To produce a lot of transferable embryos, therefore, the best conditon of culture system was co-cultured with granulosa cells for immature bovine oocytes and then co-cultured with bovine oviductal eptithelial cells for matured and fertilized oocytes. In chromosome analysis, 93% of in vitro fertilized embryos were very important aspect in chromosome preparation from bovine embryos such as duration of colcemid treatment, weakening of zona pellucida, methods of hypotonic treatment and fixation treatment.

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