• 한국임상수의학회지
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• 제25권2호
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• pp.102-105
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• 2008

The most common abnormality of the umbilicus in the foal is the patent urachus. Patent urachus may be a congenital or acquired condition in foals in which the urachus fails to close spontaneously at or shortly after parturition. A 17-day-old male Thoroughbred foal was requested to the Veterinary Pathology Laboratory of Cheju National University. The foal showed clinical signs such as umbilical urination, anorexia, depression, lethargy, and abdominal pain for 10 days. Because of the umbilical urination, the surgery for patent urachus was performed, but he died next day. Grossly, many pale yellowish foci 10-20 mm in diameter were scattered on the throughout surface of lungs. Severe subcapsular hemorrhage was observed in left kidney. Large milky yellow mass 10X6-7 cm in size was found in the adjacent area of right kidney. Histopathologically, many abscesses with bacterial cocci were scattered in the blood vessels or adjacent pulmonary parenchyma of lungs. Severe numerous abscesses with intralesional bacterial cocci were mostly occupied in the abdominal mass from right kidney. Gram staining for tissue sections demonstrated numerous Gram positive cocci in pulmonary and abdominal abscesses. In bacterial culture, catalase-positive beta-hemolytic colonies were isolated and confirmed as Staphylococcus (S.) aureus by Vitek system. Based on the results, acquired patent urachus and then multiple abscesses may be originated from the umbilical cord infected with S. aureus in this foal.

• 미생물학회지
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• 제45권1호
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• pp.86-90
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• 2009

식용얼음은 식품의 제조 가공 조리 등에 직접 사용하거나 그대로 먹기 위해 먹는 물을 얼린 얼음을 뜻하며 얼음의 제조 및 판매 과정에서 미생물이 교차 오염되면 식중독 및 장염을 유발한다. 또한 식용얼음 뿐만 아니라 상수계통, 약수물, 상수와 냉온수기의 정수된 물에서도 여러 가지 원인에 의해서 세균이 증식하는 것으로 나타났다. 이런 이유로 대구시 북구지역에서 생산되는 식용얼음의 위생 상태를 살펴보고자 무작위로 15곳을 선정하여 Brain Heart Infusion 배지에서 세균을 배양한 결과 1곳을 제외한 14곳의 검체에서 균이 증식 되었고 그람염색 결과, 그람음성간균이 80% 차지하였고 그람양성구균 17% 그리고 그람음성구균 3%의 분포를 나타냈다. 그람음성간균의 잠정적인 동정방법으로 API 20E kit와 API 20NE kit를 이용한 결과 Enterobacter cloacae, Chryseomonas luteola, Pantoea spp., Klebsiella pneumoniae, Acinetobacter baumannii, Acinetobacter calcoaceticus, Providencia rettgeri 등이 검출 되었다. 5곳에서 분리된 그람양성구균을 API Staph kit를 이용한 잠정적 동정결과 L업소에서 분리된 균은 식중독을 일으키는 병원성이 강한 Staphylococcus aureus로 동정되었다. 본 연구에서는 대구시 북구 지역의 식용 얼음류에 존재하는 일반세균 및 병원성 세균의 분포를 조사함으로 식용얼음의 위생 상태에 대한 주의를 부각시키고 나아가 대구시 타지역의 식용얼음에 대한 세균분포 조사 및 위생상태 점검에 대한 필요성을 시사한다.

• 대한임상검사과학회지
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• 제56권1호
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• pp.59-65
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• 2024

제주특별자치도에 서식하는 등줄쥐를 포획하여 대장을 포함한 내부장기에서 분변 검체를 채취한 후, 장내 미생물을 호기적 배양하여 등줄쥐 분변에서 배양된 장내 미생물 분포를 조사하였다. 등줄쥐 분변 36개의 검체를 호기성 배양된 세균 집락을 그람염색 한 결과 모든 검체에서 그람음성막대균이 우세하게 관찰되었다. 36 검체 중 그람음성막대균은 36 균주(100%), 그람양성알균은 21 균주(58.3%), 그람양성막대균은 15 균주(41.7%)로 나타났으며 그람음성알균은 관찰되지 않았다. 36개의 검체에서 배양된 그람음성막대균은 Vitek 2 system을 이용하여 동정한 결과 모두 Escherichia coli (E. coli) 균종으로 동정되었으며, 1개 검체는 E. coli 균종과 Enterobacter cloacae 균종이 동시에 동정되었다. 분리된 E. coli 36 균주 중 항균제에 내성을 보이는 균주는 발견되지 않았으며, 1 균주만 cefoxitin 항균제에 중등도 내성을 보이는 것으로 나타났다. 진드기, 설치류 매개 감염병의 원인이 되는 등줄쥐의 분변에서는 병원성 세균은 존재하지 않았다.

• Restorative Dentistry and Endodontics
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• 제19권2호
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• pp.568-584
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• 1994

Bacteria have been regarded as major etiolgic factors in root canal infections. Infected root canal flora from thirteen patients who had visited to conservative department of Wonkwang dental hospital were cultured on blood agar plates. Cultued microorganisms were isolated and identified with Gram stain and biochemical tests using Vitek Systems(BioMeriux, MO, USA); Antibiotic susceptibillity was performed with disk diffusion and broth microdilution using Vitek Systems. Gram positive cocci(65 %) were predominant, which were composed of 6 Streptococcus viridans group, 5 Staph. spp., and 4 Enterococcus faecium, in the isolatd 23 strains. Gram negative rods (26 %) were the next common bacteria, which were composed of 5 non - fermentative Gram negative rods, and 1 Enterobacter cloacae. Most strains of S. viridans group and E. faecium were susceptible to antibiotics including penicillin. But strains of Staphylococcus spp. and non - fermentative Gram negative rods showed marked resistance to antibiotics except tetrancyclin and cefotaxime. Most results between disk diffusion and microdilution were all agreed, but the results of non - fermentative Gram negative rods were susceptible to cefotaxime in disk diffusion method but resistant in microdilution.

• Archives of Pharmacal Research
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• 제13권2호
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• pp.211-213
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• 1990

To test flavonoids for antibacterial activity against oral micraorganisms, flavonoids, quercetrin and naringenin, were incorporated into two pharmaceutical preparations in the form of tooth paste. Samplees of dental plaque, the msot accused dental deposit which initiates the gingival and periodental diseases, were collected from the teeth surface of ten dental students at one week interval before and after using placebo, followed by two formulae of tooth paste containing 0.1% of quercetrin and naringenin (formulas I and II, respectively). The amount of dental plaque was assessed by the quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The amount of dental plaque was assessed by the Quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The results revealed that most of Gram negative cocci and bacilli were highly affected by the two formulae : the number of actinomycetes were decreased after using formula I and disappeared completely by the sue of formula II, while the number of Gram positive streptococci was highly decreased after the treatment with the two formulae. These results indicate a possible use of flavonoids to inhibit dental plaque formation.

• 한국환경보건학회지
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• 제19권1호
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• pp.30-36
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• 1993

A survey was conducted to measure concentration of airborne microbe in a hospital using RSC air sampler during October~November 1991.The result was as follows: 1) In an agar strip GK-A media for total counts of microbial particles. The highest count were 1384 CFU/m$^3$ in the main lobby, followed by 912 CFU/m$^3$, in the obstetric room, 688 CFU/m$^3$ in 1CU. By gram staining, the distribution for organisms in the air were shown 74.1% in gram possitive cocci followed by 16.8%, in gram possitive bacilli 6.7% in gram negative bacilli and 4.7% in yeast, but low organism was detected in recovery room with 194 CFU/m$^3$. 2) In agar strip S media for Staphylococci the count at the main lobby was detected in the recovery room with 92 CFU/m$^3$, Tests of coagulase were negative Staphylococci with 78%, and positive Staphylococci with 22%. The Staphylococci were highly resistance to penicillin, ampicillin and sensitive to amikacin, cefazolin, gentamycin and chloramphenicol. 3) In agar strip C media for coliform bacteria the colony counts at the main lobby was 139 CFU/m$^3$ and treatment room was 190 CFU/m$^3$, most frequently isolated microorganisms were non fermentative bacilli. 4) In agar strip HS media for yeast and molds. Most frequently colony counts 17~76 CFU/m$^3$, 0.5% lactophenol cotton blue stains were shown unidentified 77.2%, 8.1%, in Penicillium 8.1% in Aspergillus, and 3.8% in mucor.

• Clinical and Experimental Pediatrics
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• 제54권9호
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• pp.363-367
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• 2011

Central venous catheters (CVCs) are regularly used in intensive care units, and catheter-related bloodstream infection (CRBSI) remains a leading cause of healthcare-associated infections, particularly in preterm infants. Increased survival rate of extremely-low-birth-weight infants can be partly attributed to routine practice of CVC placement. The most common types of CVCs used in neonatal intensive care units (NICUs) include umbilical venous catheters, peripherally inserted central catheters, and tunneled catheters. CRBSI is defined as a laboratory-confirmed bloodstream infection (BSI) with either a positive catheter tip culture or a positive blood culture drawn from the CVC. BSIs most frequently result from pathogens such as gram-positive cocci, coagulase-negative staphylococci, and sometimes gram-negative organisms. CRBSIs are usually associated with several risk factors, including prolonged catheter placement, femoral access, low birth weight, and young gestational age. Most NICUs have a strategy for catheter insertion and maintenance designed to decrease CRBSIs. Specific interventions slightly differ between NICUs, particularly with regard to the types of disinfectants used for hand hygiene and appropriate skin care for the infant. In conclusion, infection rates can be reduced by the application of strict protocols for the placement and maintenance of CVCs and the education of NICU physicians and nurses.

• Journal of Yeungnam Medical Science
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• 제5권1호
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• pp.49-60
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• 1988

1984년부터 1987년까지의 최근 4년간 영남대학교 의과대학 부속병원에서 시행한 혈액배양의 분석결과 다음과 같은 결론을 얻었다. 1) 4년간 8083검체의 혈액이 배양되어 582검체에서 균이 분리되어, 총 혈액배양 양성율은 7.2%이었고, 분리균주 중 다균성 감염증은 16예(2.8%)였다. 2) 분리균주의 분포는 그람 양성균이 189, 그람 음성균이 393이었고, 그람 양성균의 균종별 분리는 Staphylococcus aureus가 82, coagulase 음성 Staphylococcus가 73, 기타 Staphylococcus sp.가 20의 순이었다. 그람 음성균은 Escherichia coli가 75, Salmonella typhi가 80, Salmonella paratyphi A가 72이고, 그 외 Kldbsiella, Enterobacter의 순으로 분리되었다. 포도당 비발효 그람 음성균은 Pseudomonas cepacia가 46, Pseudomonas aeruginosa가 16, 그 외 Acinetobacter등이 소수 분리되었다. 3) Salmonella균종을 제외한 모든 균종에서 매년 증가되는 양상을 보이고, 병원내 감염균으로 간주되는 Serratia, Acinetobacter균종이 1984년의 6.35%에서 1987년에는 17.7%롤 증가되었다. 4) Aeromonas hydrophilia는 6~10월에 높은 분리율을 나타애고, Salmonella typhi는 1월과, 5월에서 8월사이, Salmonella paratyphi A는 1월과 6월에서 9월사이에서 높은 분리율을 나타냈다. 대개 모든 균종에서 여름철에 분리율이 높았다. 5) 분리균종의 항생제 감수성 검사에서는 Staphylococcus aureus와 장내세균에서는 기본 항생제에 대한 감수성을 나타낸 반면, 포도당 비발효 균종은 약 32%에서 기본 항생제에 대한 내성을 가진 것으로 나타났으며, 병원성 감염의 주요 원인균은 Serratia, Acinetobacter, Pseudomonas, Enterobacter균종에서는 항생제의 내성이 큰 문제점으로 대두되었다.

• 대한간호학회지
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• 제12권1호
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• pp.39-52
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• 1982

Nosocomial infection is important in the management of the nursing care and has been found to be related with the airborne microbes contamination. The purpose of this study is to identify the differences of the airborne microbes between hospitals, nursing units, days and times and to identify the difference in the types of microorganisms between hospitals, nursing units, days and times. This study was conducted from May 25, to July 10, 1981, according to R. Koch's methods for quantative samplings of airborne microbes and the results of this study were reviewed in a statistical method. The following conclusions were obtained: 1. There was a significant difference in the types of airborne microbes between 8 hospitals (F=5.0491, p<0.01) 2. There was a significant difference in the types of airborne microbes between surgical, medical, nursery, 1.C.U., operating theatre and outpatient nursing unit. (F=2.1764, p<0.05) 3. There was not a significant difference in the types of airborne microbes between Monday, Thursday and Saturday (F= 1.6365, p>0.05) 4. There was a significant difference in the types of airborne microbes between AM 7 : 00, AM 11：00 and PM 3 : 00. (F=7.2951, p<0.01) 5. 4061 colonies were divided into more 48 types and the classification was as follows. gram positive cocci (2024 colonies) 49.3% gram positive bacillus (1211 colonies) 29.8% gram negative bacillus (577 colonies) 14.2% fungus(200colonies) 4.9% gram negative cocci (41 colonies) 1.0% other's(8 colonies) 0.3% 6. There was a significant difference of airborne microbes between 8 hospitals. (F=7.7943, P<0.01) 7. There was not a significant difference of airborne microbes between 6 nursing units. (F= 1.8461, P>0.05) outpatient nursing unit : 12.6 colonies surgical nursing unit. 10.4 colonies Medical nursing unit : 9.8 colonies nursery nursing unit : 9.4 colonies operating theatre nursing unit：8.2 colonies Intensive care nursing unit : 7.8 colonies 8. There was not a significant difference of airborne microbes between Monday, Thursday and Saturday. (F=1.4371, p>0.05). Saturday : 11.0 colonies Monday : 9.1 colonies Thursday : 9.1 colonies 9. There was a significant difference of airborne microbes between A. M. 7 ; 00, A. M. 11 : 00 and P .M. 3：00 (F=7.6658, p<0.05) P. M. 3 : 00 : 12.2 colonies A. M. 11 : 00 : 10.0 colonies A. M. 7 : 00 : 7.1 colonies.

• 대한미생물학회지
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• 제20권1호
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• pp.13-23
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• 1985

This investigation was performed to isolate and identify Staphylococcus sp. from air at the 25 sites in one hospital in Daegu. Drug sensitivity tests bacteriologically were also studied. The results are summarized as follows: 1. In gram staining of isolated colonies, the number of staphylococci strains was 959(70.2%) among 1367 collected in the morning, and 653(66.2%) among 987 collected in the afternoon. 2. The number of isolates was slightly higher in samples collected from the outpatient treatment rooms and wards in the main building than in samples from the other sites. 3. In biochemical tests of 1612 strains of isolates gram positive cocci, the number of coagulase positive strains was 584(36.2%) and coagulase negative 1028(63.8%). In experiments on growth on mannitol agar containing 7.5% NaCl and mannitol fermentation tests, the number of mannitol fermentation strains was 423(30.8%) and nonfermenter was 951(59.2%). 4. In antibiotic sensitivity tests of 746 identified strains of staphylococci, coagulase positive strains showed resistance in. higher portions to penicillin(89.7%), chloramphenicol(64.6%), gentamycin (52.1%) and tetracycline(45.2%), whereas in lower portions to kanamycin(39.2%) and apmicillin(33.1%). 5. Among coagulase negative and mannitol fermenting strains, higher portions showed resistance to chloramphenicol(76.5%), penicillin(69.8%) and ampicillin(63.6%), whereas lower portions showed resistance to kanamycin(48.8%), tetracycline(39.5%) and gentamycin(34.0%). 6. The number of strains showing multiple resistance to above 6 antibiotics were 15(52(19.8%) among coagulase positive and mannitol non-fermenter and 36(22.2%) among coagulase negative and mannitol fermenter.