• Journal of Korean Society of Environmental Engineers
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• v.32 no.12
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• pp.1141-1146
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• 2010

Isolated the heterotrophic aerobic bacteria in sandfiltered water on NA and TSBA solid medium, selected 8 dominant species and identified by Sherlock System. Each samples are irradiated 0, 5, 16, 40 and $60\;mJ/cm^2$ using on CBD (Collimated Beam Device) Medium Pressure UV lamp after these identified bacterium did liquid culture how to make $10^6{\sim}10^7\;cells/mL$ suspended in dilution water. Then cultured bacteria are estimated inactivation rate on plate media. Identified Gram positive group are Bacillus Subtilus, Bacillus megaterium, Rhodococcus erythropolis and Microbacterium laevaniformans; Gram negative group are Pseudomonas vesicularis, Pseudomonas pseudoflava, Alcaligenes paradoxus and Zooglea ramigera. These isolation of bacterium are more stronger reference strain and high resistance of MP UV irradiation, Besides Gram negative bacterium are more sensitive Gram positive bacterium on MP UV dose. Now we are estimating to $60{\sim}100\;mJ/cm^2$ MP UV dose for efficient disinfection in water treatment plant.

• Microbiology and Biotechnology Letters
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• v.24 no.2
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• pp.160-165
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• 1996

An alkalophilic bacterium producing alkaline protease(s) was isolated from soil. It was a Gram-positive, non-sporulating, immotile, irregular rod, strictly aerobic, and weak acid-forming bacterium. The morphological, physiological, and biochemical characteristics of the isolate resembled those of the Coryneform bacteria. However, there was not any species within this genera to which this microorganism can be closely matched. Therefore, it is provisionally identified as a Coryneform bacterium TU-19.

• Korean Journal of Pharmacognosy
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• v.18 no.1
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• pp.1-4
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• 1987

The methanol and benzene extracts of the leaves of 55 higher plants in Korea were tested for their antibacterial activity against three Gram positive bacteria, Streptococcus mutans, Staphylococcus aureus and Bacillus subtilis, and one Gram negative bacterium Escherichia coli. Among them, the methanol extract of the leaves of Liriodendron tulipifera showed remarkably potent antibacterial activity against both Gram positive and negative bacteria.

• The Microorganisms and Industry
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• v.17 no.1
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• pp.10-18
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• 1991

A phylogenetic comparison of homologous protein can often supplement genetic and biochemical analysis by revealing conserved structures that are critical for function(Waugh et al., 1989). I therefore isolated a secY homologue from B. subtilis, a gram positive bacterium evolutionary distant from E. coli. The comparison and interplay between these two bacterial systems should contribute greatly to our understanding of the functions and interactions within systems evolved for protein translocation in both prokaryotic and eukaryotic organisms.

• Biodesign
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• v.6 no.4
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• pp.96-99
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• 2018

The gram-positive bacterium Staphylococcus aureus is a common cause of abscesses, sinusitis and food poisoning. The emergence of antibiotic-resistant strains has caused significant clinical issues worldwide. The HslU-HslV complex was first identified as a prokaryotic homolog of eukaryotic proteasomes. HslU is an unfoldase that mediates the unfolding of the substrate proteins, and it works with the protease HslV in the complex. To date, the protein complex has been mostly studied in gram-negative bacteria. In this study, we report the purification and crystallization of the full-length HslU from S. aureus. The crystal diffracted X-rays to a $3.5{\AA}$ resolution, revealing that the crystals belong to space group $P2_1$, with unit cell parameters of a = 166.5, b = 189.6, $c=226.6{\AA}$, and ${\beta}=108.1^{\circ}$. We solved the phage problem by molecular replacement using the structure of HslU from Haemophilus influenzae as a search model. The cell content analysis with this molecular replacement solution revealed that 24 molecules are contained in the asymmetric unit. This structure provides insight into the structural and mechanistic difference of the HslUV complex of gram-positive bacteria.

• The Korean Journal of Food And Nutrition
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• v.12 no.6
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• pp.559-563
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• 1999

Essential oil of Artemisia lavandulaefolia the chrysanthemum family plant used in the chinese medicine was extracted and antibacterial and antifungal activity with many kinds of the pathogenic bacterium and fungi was experimented by it. Ataphylococcus epidermidis and Streptococcus aureus gram positive bacterium at the concentration of 200ppm and Streptococcus mutans at the concentration of 1,000ppm showed the growth injibition effect of the cell. These showed statistically significant difference(p<0.05) Zymomonas mobilis Entrecoccus faecalis gram negative bacterium at the concentration of 200ppm and Pseudomonas putida at the concentration of 400ppm showedd the growth inhibition effect of the cell)p<0.05) V. Parahaemolyticus at the concentration of 800ppm and Pseudomonas aeruginosa at the concentration of 1,000ppm showed the growth inhibition effect of the cell(p<0.05) Candida albicans and Cryptococcus neoformans yeast-type fungi showed the gorwth inhibition effect of the cell at the concentration of 200ppm(p<0.05) Altenaria mali Aspergillus nidulans and Fusarium oxysporum filamentous fungi took the growth inhibition effect of the cell at the concentration of 600ppm, 400ppm, and 100ppm. respectively.

• Ceramist
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• v.22 no.3
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• pp.301-315
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• 2019

We prepared hybrids between layered double hydroxide (LDH) and natural plant extract such as Peaonia suffruticosa Andrews (PS) and Peaonia Japonica (PJ) which was confirmed anti-bacterial activity through paper disc diffusion assay. According to X-ray diffractometer, scanning electron microscope, zeta-potential measurement and quantification of extract loading amount in hybrids, we confirmed that similar amount of PS and PJ loaded on inter-particle pore of LDH with partial adsorption on surface of LDH through reconstruction process. We also evaluated the bacterial colony forming inhibition of PS extract, PJ extract, PS-LDH and PJ-LDH hybrids against Escherichia coli as gram negative bacterium and Bacillus subtilis as gram positive bacterium, suggesting that both hybrids have enhanced anti-bacterial activity compared with extract itself.

• Journal of the Korean Chemical Society
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• v.48 no.2
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• pp.144-150
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• 2004

Various experimental factors that affect the separation of bacteria were investigated using capillary electrophoresis. At different buffer concentrations, gram-positive bacteria and gram-negative bacteria showed somewhat different migration behavior under high electric filed. The separation efficiency was also investigated as a function of concentration of bacterium injected into the capillary. In order to separate bacteria as the difference of size and shape, water soluble polymers such as poly(ethylene)oxide (PEO), polyvinylpyirrolidone (PVP), and dextran were studied. PEO, which is more flexible and has lower steric hinderance, showed the best separation efficiency. The mixed bacteria sample of Micrococcus lysodeikticus as gram-positive bacteria and Aerobacter aerogenes as gram-negative bacteria were successfully analyzed with PEO.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.1
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• pp.193-195
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• 2001

A radiation-resistant bacterium was isolated from gamma irradiated dried laver (Porphyra tenera) and its microbiological characteristics were examined. As a result of resistance test to gamma irradiation, the isolate was survived $10^{3}$ CFU/mL even at 30 kGy and significant shoulder line zone was shown until 20 kGy. The $D_{10}$ value was 11.27 kGy. The isolate was gram-positive, non-motile coccus and catalase-positive. n culture, the red-pigmented smooth colony was observed. The biochemical test in API (analytical profile index) system showed that the isolate fermented glucose and fructose as the carbon source. Therefore, a radiation-resistant bacterium isolated from laver was potentially identified as Micrococcus roseus sp.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.343-346
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• 2005

The isolated strain, SC2-1 was Gram-positive, catalase positive, facultatively anaerobic, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and sodium chloride required for the bacteria growth. The radical scavenging activity of the culture supernatants was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This bacterium was identified based on cellular fatty acids analysis and 16S rDNA sequencing then named Exiguobacterium sp. SC2-1. The optimum culture conditions for production of antioxidant were $25^{\circ}C,$ pH 7.8 and NaCl concentration were 4%. The modified optimal medium compositions were maltose 2.5% (w/v), yeast extract 1.5% (w/v) and $KH_2PO_4$ 0.05% (w/v). Free radical scavenging activity of under optimal culture conditions were 93%.