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http://dx.doi.org/10.5012/jkcs.2004.48.2.144

Separation of Bacteria Using Capillary Electrophoresis  

Moon, Byoung-Geoun (Division of Chemical Engineering, Kyungnam University)
Choi, Kyu-Seong (Division of Chemical Engineering, Kyungnam University)
Lee, Sang-Chun (Division of Chemical Engineering, Kyungnam University)
Kim, Yong-Seong (Division of Chemical Engineering, Kyungnam University)
Publication Information
Journal of the Korean Chemical Society / v.48, no.2, 2004 , pp. 144-150 More about this Journal
Abstract
Various experimental factors that affect the separation of bacteria were investigated using capillary electrophoresis. At different buffer concentrations, gram-positive bacteria and gram-negative bacteria showed somewhat different migration behavior under high electric filed. The separation efficiency was also investigated as a function of concentration of bacterium injected into the capillary. In order to separate bacteria as the difference of size and shape, water soluble polymers such as poly(ethylene)oxide (PEO), polyvinylpyirrolidone (PVP), and dextran were studied. PEO, which is more flexible and has lower steric hinderance, showed the best separation efficiency. The mixed bacteria sample of Micrococcus lysodeikticus as gram-positive bacteria and Aerobacter aerogenes as gram-negative bacteria were successfully analyzed with PEO.
Keywords
Capillary; Electrophoresis; Bacteria; Polymer;
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