• Journal of Species Research
• /
• v.6 no.2
• /
• pp.91-100
• /
• 2017

Five bacterial strains, ES10-3-3-1, KKM10-2-2-1, Ant11, JM10-4-1-3, and KMS4-11 assigned to the genus Deinococcus were isolated from soil samples collected from Namyangju-si in Gyeonggi-do, Gangnam-gu and Dongdaemun-gu in Seoul, Korea. In addition, four bacterial strains, KKM10-2-7-2, JM10-2-5, JM10-2-6-2, and KKM10-2-3 assigned to the genus Hymenobacter were isolated from soil samples collected from Gangnam-gu and Dongdaemun-gu in Seoul, in South Korea. The five Deinococcus species were Gram-stain positive, pink-pigmented, and short-rod or coccus shaped. The four Hymenobacter species were Gram-stain negative, red-pigmented, and short-rod shaped. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ES10-3-3-1, KKM10-2-2-1, Ant11, JM10-4-1-3, and KMS4-11 were most closely related to Deinococcus citri $NCCP-154^T$ (with 99.8% similarity), Deinococcus grandis DSM $12784^T$ (99.0%), Deinococcus marmoris DSM $12784^T$ (98.8%), Deinococcus claudionis $PO-04-19-125^T$ (98.7%), and Deinococcus radioresistens $8A^T$ (99.8%), respectively. KKM10-2-7-2, JM10-2-5, JM10-2-6-2, and KKM10-2-3 were most closely related to Hymenobacter algoricola $VUG-A23a^T$ (99.1% similarity), Hymenobacter elongatus $VUG-A112^T$ (99.1% similarity), Hymenobacter gelipurpurascens $Txg1^T$ (99.1% similarity), and Hymenobacter psychrotolerans $Tibet-IIU11^T$ (99.3% similarity), respectively. These nine species have never been reported in Korea; thus, five Deinococcus species are reported in the family Deinococcaceae, order Deinococcales, class Deinococci, phylum Deinococcus-Thermus and four Hymenobacter species are reported in the family Cytophagaceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes.

• The korean journal of orthodontics
• /
• v.19 no.2
• /
• pp.57-73
• /
• 1989

The purpose of this study was to investigate the tissue response of the rat molar periodontium incident to intermittent orthodontic force. The author intended to observe the healing process of injured periodontium and the response of injured tissue to the resumed force. Oxytetracyclin 50mg/Kg was given to each rat intraperitonially. 5 days later, maxillary 1st molars were moved mesially from the incisors with closed coil spring of 100gram. 7 days later, the appliances were removed and 20mg/Kg of calcein were given intraperitonially to each rat. At the same time, maxillary left 1st molars of 15 rats were moved by the same method, but force was lowered to 20 gram. After 1 day, maxillary left 1st molars of another 15 rats were moved by the same method and 50mg/Kg of oxytetracycline was given intraperitonially. After 4 days, another 15 rats were treated as above. After 7 days, another 15 rats were treated as above. 1,4,7,10 and 14 days after change of force, 3 rats were sacrificed in each group respectively. 2 rats were decalcified, embedded in paraffin, and stained with hematoxylin-eosin stain and with Masson's trichrome stain. Another rat was embedded in polyester resin and undecalcified specimen were made. Microradiograms were taken with the undecalcified sections. Observations were made with light and fluorescence microscope. Following conclusions were made. 1. Connective tissue cells and vessels were infiltrated into the hyalinized tissue from the bony cleft and along the border of the hyalinized tissue with bone and root surface. At the same time, elimination of hyalinized tissue, bone and root resorption occurred. 2. Bone and root were resorbed directly and indirectly. 3. Hyalinized tissue was removed within 5 days after force removal. 4. Hyalinized zone was less extensive and easily removed as the rest period prolonged. 5. Hyalinized tissue developed more rapidly and extensively and lasted over 10 days as the force resumed on the already formed hyalinized tissue.

• Korean Journal of Veterinary Research
• /
• v.39 no.4
• /
• pp.819-824
• /
• 1999

Suspected infections of Campylobacter spp and Clostridium spp were observed in three dogs. The diagnosis was based on fecal cytology, Gram's stain, clinical signs and serum chemistry. The rectal swabs of diarrheic dogs were performed to confirm the enteropathogens. Suspected Campylobacter spp were a sea-gull shape and Clostridium spp had a large, clear endospore in rectal cytology. Treatment with appropriate antibiotics resulted in a complete resolution of all clinical abnormalities in three cases. The source of Campylobacter spp and Clostridium spp could not be found clearly in three cases, but gastrointestinal origin was most likely. When detecting the enteropathogens in feces, fecal smear with Wright's and Gram's stain should be made at first and also, if the patients have canine parvoviral enteritis, attention should be paid to confirm the Campylobacter spp and Clostridium spp. In addition, since Campylobacter spp and Clostridium spp as normal bacterial flora exists in canine intestines, it is thought that microbiological isolation should be performed to confirm the suspected Campylobacter spp and Clostridium spp as primary enteropathogens in subsequent study.

• Journal of the Korean Society of Radiology
• /
• v.18 no.4
• /
• pp.365-372
• /
• 2024

To analyze the image quality and disinfection effectiveness of alcohol gel application in ultrasound scans, we varied the application of alcohol gels with different alcohol content from ultrasound gels. The image quality analysis compared the Dead zone, axial and lateral resolution, sensitivity, and gray scale changes using the ATS-539 phantom, while the disinfection effectiveness analysis was performed by swabbing the three cover faces of convex probe used in clinical practice, collecting specimens and enrichment culture them for 24 hours before identification with Gram stain. image quality analysis showed that all items, regardless of the alcohol content, produced images that were not different from the ultrasonic gel application images, and pathogens such as staphylococcus aureus, streptococcus, and enterococcus were detected on all probes, and all were extinction by the application of alcohol gel. The application of alcohol gel during ultrasound examination was meaningful in terms of image quality maintenance and disinfection effect, and if the results of this study are further studied and commercialized, the application of alcohol gel as an alternative to ultrasound gel will be very useful in clinical examinations because it can consider not only image quality but also infection prevention.

• Journal of Sericultural and Entomological Science
• /
• v.7
• /
• pp.53-63
• /
• 1967

In order to identify the bacteria living on the cocoons in Korea, the isolated bacterias' morphological. cultural and physiological characters has been determined through the detailed study. The second aim of this experiment was to protect against the bacteria which damage silk protein during storage. 1. The twelve strains of the bacteria were isolated and identified in the cocoons produced in Korea. The results of the identification are as the following. No 1, No 8; Bacillus subtilis variation No 2, ; Bacillus stearothermophilus No 3, ; Bacillus circulans No 5, No 6; Bacillus thuringiensis No 7, No 11; Bacillus brevis No 12, No l0; Bacillus cereus variation

• Korean Journal of Veterinary Pathology
• /
• v.1 no.1
• /
• pp.66-71
• /
• 1997

Eighteen NZW rabbits used for local skin irritation study were examined grossly and microscopically for natural infection with Encephalitozoon cuniculi. For microscopic tissue evaluation histochemical techniques such as PAS Gram iron hematoxylin and HE stain were used. Although rabbits in the study had no abnormal clinical signs 7/18(38.9%) animals were microscopically infected with E. cuniculi. The affected rabbits had gray-whitish and depressed approximately 0.1∼0.6 cm diameter lesions in the kidneys. All other organs examined were grossly unremarkable. Histopathologically however in addition to segmental interstitial nephritis focal lymphocytic myocarditis and granulomatous inflammatory reaction in portal areas of the liver multifocal granulomatous foci with vasculitis were present in the brain kidney and lungs. Aggregates of minute oval organisms were observed in brain and kidney sections frequently within the granulomatous foci and sometimes without any inflammatory reaction particularly in the renal tubules. in histochemical stains the organisms were gram positive stained with iron hematoxylin and had PAS positive granule at one pole. They measured approximately 1.5×2.5μm consistent with E. cuniculi. Histochemical characterization is important to differentiate E. cuniculi from other common protozoal infection such as Toxoplasma gondii. This study demonstrate the importance of subclinical. E. cunuculi infection and the associated histological alterations may interfere with tissue evaluation in toxicologic studies.

• The Korean Journal of Cytopathology
• /
• v.6 no.2
• /
• pp.169-173
• /
• 1995

Nocardia, aerobic members of the order of Actinomycetaceae, produces infections in human lung. Nocardial infection is associated with underlying diseases of immuno-suppression or treatment with corticosteroid. It is difficult to detect Nocardia by sputum examination or histologic sections and it has rarely been diagnosed by fine needle aspiration of the lung. We describe a case of pulmonary nocardiosis in a 72 year-old man, diagnosed by fine needle aspiration, which was confirmed by culture of aspirates. The aspirates showed neutrophil-predominant inflammatory cells with microorganisms demonstrated by Gomori methenamine silver and Gram stain. The organisms had characteristic long blanching filamentous structures. The lesions on chest X-ray were in resolution with antimicrobial therapy.

• Korean Journal of Veterinary Pathology
• /
• v.7 no.1
• /
• pp.67-69
• /
• 2003

Three mature layer chickens from a farm in which chickens showed green diarrhea, cyanosis, lethargy, loss of appetite were pathologically examined. Grossly, multiple variable sized caseous nodules were detected in the liver, intestinal serosa and mesentery. In addition, parathypoid nodules in the liver and fibrous serositis on the several peritoneal organs and tissues were noticed. One of spleens had multiple infarction areas. Histologically caseous nodules consisted of central caseous core and peripheral epithelioid cells overlying the fibrous connective tissue. Multinucleated giant cells were scattered between the epithelioid cells and fibrous connective tissue. In these nodules Gram negative cocobacilus bacterial colonies were present, whereas Periodic Schiff reaction and Ziehl-Neelsen stain detected neither fungi nor acid fast bacteria. From these results multiple granulomas might be induced by Escherichia coli. In addition, severe Ascafdiodf and Salmonellosis were coinfected in these chickens.

• Restorative Dentistry and Endodontics
• /
• v.11 no.1
• /
• pp.97-103
• /
• 1985

The purpose of this study was to compare Black-pigmented Bacteroides isolated from necrotic pulp with the presence or absence of eight symptoms associated with pulpal necrosis and to identify the proportion of each Black-pigmented Bacteroides species. The canal contents of fourteen traumatically and cariously necrotized teeth were sampled with a special technique and cultured aerobically and anaerobically for growth in blood agar plate and for Black-pigmented Bacteroides on selective blood agar plate. Each Black-pigmented Bacteroides species were identified by Gram's stain, hemolysis reaction, colony color and morphology and biochemical tests. The results were as follows; 1. 60.9 percent of the bacteria isolated were anaerobic and 39.1 percent were aerobic. 2. Four Black-pigmented Bacteroides species were isolated; B. loescheii (74.1%), B. denticola (11.1%), B. intermedius (7.4%) and B. gingivalis (7.4%). 3. Black-pigmented Bacteroides was found to be significantly related to sinus tract formation and exudate.

• The Journal of the Korean dental association
• /
• v.10 no.10
• /
• pp.654-658
• /
• 1972

The toplications of 50% nicotin paste in oral mucous membrane of male mice weigh-ingabout 30 gram, were executed for 10, 15 and 20 days. And the tissues were observed in HE and PAS stain. The findings observed are as follows; I. Hyperplastic changes were revealed in underlying connective tissue and spinous cell layer. There were moderate vasodilatations with out edema but PAS reactions were dictinctive. in 10th day of experiment. II. There was remarkable increment of mortality up to 40percent and weight loss, but microscopic findings were not significant differences on than 10th days, in 15th day of experiment. III. Cellular anisocytosis, hyperchromatism and degenerations were observed in spinous cell layer, and vascular engergement in connec tivetissues were observed. And the mortality was up to 70 percent in 20th day of experment.