• Proceedings of the Korean Society of Crop Science Conference
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• 2004.04a
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• pp.12-27
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• 2004

Thanks to spectacular advances in the techniques for identifying proteins separated by two-dimensional electrophoresis and in methods for large-scale analysis of proteome variations, proteomics is becoming an essential methodology in various fields of plant sciences. Plant proteomics would be most useful when combined with other functional genomics tools and approaches. A combination of microarray and proteomics analysis will indicate whether gene regulation is controlled at the level of transcription or translation and protein accumulation. In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is a most prevalent technique to identify rapidly a large of proteins in proteome analysis. However, the conventional Western blotting/sequencing technique us still used in many laboratories. As a first step to efficiently construct protein data-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein spots are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins (i. e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 30% of total rice cDNA have been deposited in the database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that fumed out to be calreticulin, gibberellin-binding protein, which is ribulose-1,5-bisphosphate carboxylase/oxygenase activate in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins (http://genome .c .kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Recently, we are separated proteins from grain filling and seed maturation in rice to perform ESI-Q-TOF/MS and MALDI-TOF/MS. This experiment shows a possibility to easily and rapidly identify a number of 2-DE separated proteins of rice by ESI-Q-TOF/MS and MALDI-TOF/MS. Therefore, the Information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful in the plant molecular breeding. Also, information from our study could provide a venue to plant breeder and molecular biologist to design their research strategies precisely.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.205-209
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• 2021

Barnyard millet (Echinochloa esculenta) can be processed through soiling, hay, and silage, depending on the weather conditions during harvesting. However, research on barnyard millet is insufficient, and standards for cultivar, seeding density, and fertilizers have not been established. This study was conducted to examine the effects of seeding density and seeding methods on dry matter yields. For this, we used the early-maturing (Shirohie millet) variety of the barnyard millet. The experimental design included different seeding density (10 kg/ha, 15 kg/ha, 20 kg/ha (standard seeding density), 25 kg/ha and 30 kg/ha), and different methods of seeding (drill seeding and broadcast seeding). The seeding date was May 13, 2021, and the harvest date was July 13, 2021. Harvesting was carried out when the heading reached 40 %. Lodging occurred at 5, 9 and 7 at 20, 25 and 30 kg/ha densities in the broadcast seeding, but not in the drill seeding. With decreasing density of seeding, tillage number showed an increasing trend in both drill seeding and broadcast seeding (p>0.05). The plant heights were comparable in both drill seeding and broadcast seeding (p>0.05). The heading stage of barnyard millet was checked July 7 for drill seeding, and, on July 8 for broadcast seeding. It took 62 days, i.e., till July 13 for the heading to reach 40 % of the output. The dry matter yield of barnyard millet was significantly higher at the seeding density of 30 kg/ha, for both the methods of seeding (p<0.05). There was no difference in the chemical composition of grain, based on the seeding method and seeding density. However, as the seeding density increased, the CP, NDF, ADF, and TDN contents increased in both drill seeding and broadcast seeding. We found that, the dry matter content was the highest at the seeding density of 30 kg/ha for both the methods of seeding, but this was only 1.3 times higher than that of 10 kg/ha. Considering the seed price and labor force involved in seeding, it is advisable to have a seeding density of 15-20 kg/ha.

• Journal of the Korean Geotechnical Society
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• v.34 no.12
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• pp.105-113
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• 2018

In this study, the engineering properties including bearing capacity of subgrades stabilized with a binder are analyzed by laboratory and field experiments. The main components of the binder are CaO and $SO_3$. After the binder was mixed with a low plasticity clay, the passing rates were relatively decreased as the sieve mesh size increased. Not only did the soil type change to silty sand, but engineering properties, such as the plasticity index and modified California bearing ratio (CBR), were improved for the subgrade. A comparison of the compaction curves of the stabilized subgrade and field soil compacted with the same energy demonstrated an increase of approximately 6% in the maximum dry unit weight, slight decrease in optimum moisture content, and considerable increase improvement in grain size. In the modified CBR test, the effect of unit weight and strength increase of the modified soil (with a specific amount of binder) was remarkably improved. As the proportion of granulated material increased after the addition of binder, the swelling was reduced by 3.3 times or more during initial compaction and 6.5 times by final compaction. The unconfined compressive strength of the specimens was maintained at the homogeneous value with a constant design strength. The stabilized subgrade was validated by applying it in the field under the same conditions; this test demonstrated that the bearing capacity coefficients at all six sites after one day of compaction exceeded the target value and exhibited good variability.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.13
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• pp.119-126
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• 1973

In order to investigate the effects of phosphorous fertilizer such as fused Phosphate, compound fertilizer and triple phosphate on barley(growth and yield at the fixed level of nitrogen and potassium, 4 level of hosphorous) has been tested at Suwon. Iri. Kwangju ani Daegu with randomized completeblock design. The results obtained could be summarized as follows. 1. In four locations. increased application of phosphorus have brought earlier maturity in barley regardless the type of fertilizer but fused phosphate considered to have more effects on stimulation of maturity of barley compare with the others. 3. Fused phosphate increased yield higher by-8kg/10a application compare with the same level of compound fertilizer and triple phosphate but there was no differences in yield at 12kg/10a phosphate but there was no differences in yield at 12kg/l0a application among the fertilizers except Kwangju and Suwon. 3. Grain weight was considerably higher in application of fused phosphate Thus are considered effects phosphate. 4. Optimum amount of phosphorous fertilizer was considered 8kg/l0a. Application of 4kg per 10a produced lower yield than 8kg and slightly or no increased yield was observed in 12kg application. 5. Among the 4kg/10a applied condition of phosphorous fertilizers, yield was decreased at fused phosphate treatment compare with the others and this fact resulted supposedly due to the lower portion of valid phosphorus in fused phosphate because of its citric acid solubility. At level of 12kg/10a application fused phosphate was considered more effective in yield increment.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.92-92
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• 2022

In preparation for the ever-changing climate and unification of North Korea and South Korea, it is necessary to increase the grain self-sufficiency rate by selecting crops with good utilization in high-altitude regions. The principle is to sow pastures at the end of August. However, sowing occurs in spring because the sowing period is missed when the weather is bad or when the workforce is insufficient. Sometimes when the grassland is completely devastated, it is frequently sowed in spring. In addition, North Korea consists of a high-altitude regions, and has been devastated in a general mountainous region. As a result, the landscape is not good and it is vulnerable to natural disasters such as landslides. Therefore, to prevent this, pasture must be sowed in the high-altitude regions. The goal of this study was to evaluate and compare yield and feed value of pasture species and varieties by spring sowing in high-latitude regions. The study was conducted in Pyeongchang, Gangwon-do, which is 700m height above sea level. The pasture species and varieties was sown on April 24, 2022. Each treatment was carried out by sowing 30 kg/ha, the experiment field size was 1 m²(1m×1m), and randomized block design with tri-repeat. The total of 14 varieties was used in the study, 6 varieties of Orchardgrass (OG), 6 varieties of Tall fescue (TF) and 2 varieties of Perennial ryegrass (PRG). The grassland composition fertilization using (N:P₂O₅:K₂O at 80:200:70 kg/ha) was conducted and management fertilizer was N:P₂O₅:K₂O at 210:150:180 kg/ha. The first harvest was June 26,2022 and the second harvest was on August 16, 2022. For statistical analysis of the data, an Analysis of Variance (ANOVA) was performed using the R3.6.3 software program, and all data was subjected to analysis using Duncan's multiple range test. Significance was set at the 5% level. The dry matter yield at the first harvest was the highest in PRG, and second harvest was the highest in TF (p < 0.05). Overall, PRG showed a trend of gradually decreasing growth, OG and TF showed a trend of gradually improving growth. This showed that PRG was considerably weaker to summer depression than other pasture species. Comparing the total dry matter yield, TF was the highest (4,565.45 kg/ha), but there was no significance difference with PRG (4,487.24 kg/ha) (p < 0.05). In addition, comparing the total TDN (total digestible nutrient) yield, TF was the highest (3147.33 kg/ha), second in PRG (2975.67 kg/ha) and third in OG (2052.33 kg/ha). Since this result is the data of the second harvests, if the result is derived by the end of next year, it will be provided as basic data for selection of pasture species and varieties suitable for spring seeding in high-altitude regions.

• Animal Bioscience
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• v.37 no.8
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• pp.1474-1482
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• 2024

Objective: The experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice. Methods: Twenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results: The coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy, in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine, threonine (Thr), and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr, and Trp were as following, SID_CP = -664.181+8.484×DM (R² = 0.40), SID_Lys = 53.126+6.031×ether extract (EE)+0.893×thousand-kernel volume (R² = 0.66), SID_Thr = 39.916+7.843×EE (R² = 0.41), and SID_Trp = -361.588+4.891×DM+0.387×total starch (R² = 0.85). Conclusion: Overall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

• Korean Journal of Soil Science and Fertilizer
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• v.3 no.1
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• pp.35-41
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• 1970

The experiment was conducted at the salt concentration of 0.5% and 1% end of April, respectively, in low and high-salty and the non-salty areas of silt loam with the Nongkwang, rice variety. The factorial design with confounding blocks of 3 levels each of 10, 15 and 20 kg of N, 8, 12 and 16kg of phosphate and potash, respectively, per 10a was applied. 1. N applications increased by 1.5 and 2 times with the fixed amount of $P_2O_5$ and $K_2O$ (8kg/10a each) increased the proportion absorbed to the applications of N in both non salty and low-salty areas. It was observed that the absorption of Ca and Si was inhibited by either an increased treatment of N alone or combination with the other nutrients in the salty area. 2. In the non-salty area, an increased applications of standard amount of N, $P_2O_5$ and $K_2O$ respectively did not increased the yields. Doubling the application of $K_2O$ resulted in a decreased yield. 3. Applications of additional of 1.5 and 2 times the 10 kg of N per 10a increased the rice yields 12% and 21% respectively, in the low-salty area. An increased application of $P_2O_5$ and $K_2O$ failed to bring about an increased yield. 4. Increasing the application of N gave a significant increased in the yield of rice grain and 1.5 times of N applications were seemed profitable on the high-salty area. Although an increased applications $P_2O_5$ and $K_2O$ seemed to increase the yields of grain, no significant increase was observed. 5. An increased application of N increased the number of panicles up to 1.5 times the standard amount in the non-salty area, but no further increase resulted by doubling the application. The number of panicles was increased in proportion to the increased application of N in both low and high-salty areas. An increased application of $P_2O_5$ increase the number of panicles per unit area in each experimental plot while that of $K_2O$ had no effect but rather decreased the number. 6. The effect of an increased application of N decreased the weight of panicle in the non-salty area, but when the application was increased to 1.5 times or more an increased weight of panicle resulted in both salty areas. Doubling the application had approximately the same effect as 1.5 times the application. Increasing the applications of $P_2O_5$ and $K_2O$ had no effect on the panicle weight in the experimental plots. Increasing the applications of N, $P_2O_5$ and $K_2O$ did not effect the weight of 1,000 grains produced in the non-salty and salty areas. Increasing the application of N decreased the number of grains per panicle in the non-salty area but increased the number of grains per panicle in either salty areas. 7. The ratio of matured grains was highest in the low-salty area and the lowest in the high-salty area. An increased N applications decreased the ratio of matured grains in the non-salty area. No effect was observed in both low and high-salty areas. Increased the $P_2O_5$ and $K_2O$ application showed no effect on the ratio of matured grains in the experimental plots. 8. Increased applications of N, $P_2O_5$ and $K_2O$ was observed not to change the percentage of milling recovery in any experimental plots. Broken rice was increased equally by an increased application of N in the non-salty and salty areas but more remarkably so in the former. 9. Increased applications of N increased the straw production equally in the non-salty, low and high-salty areas. However, no increased production was observed from heavier applications of $P_2O_5$ and $K_2O$. Additional N applications reduced the rate of rough grain weight v.s. straw weight in the non-salty area but increased the ratios in both low and high-salty areas. Additional $P_2O_5$ and $K_2O$ had no effect with the ratio.

• Korean Journal of Agricultural Science
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• v.4 no.2
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• pp.254-282
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• 1977

To obtain information on the inheritance of the quantitative characters related with the vegetative and reproductive growth of rice, the $F_1$ seeds were obtained in 1974 from the all possible combinations of the diallel crosses among five leading rice varieties : Nongbaek, Tongil, Palgueng, Mangyeong and Gimmaze. The $F_1$'s including reciprocals and parents were grown under the standard cultivation method at Chungnam Provincial Office of Rural Development in 1975. The arrangement of experimental plots was randomized block design with 3 replications and 12 characters were used for the analysis. Analytical procedure for genetic components was followed the Griffing's and Hayman's methods and the results obtained are summarized as follows. 1. In all $F_1$'s of Tongil crosses, the longer duration to heading was due to dominant effect of Tongil and each $F_1$ showed high heterosis in delaying the heading time. It was assumed that non-allelic gene action besides dominant gene effect might be involed in days to heading character. However, in all $F_1$'s from the crosses among parents excluding Tongil the shorter duration was due to dominant gene action and the degree of dominance was partial, since dominance effects were not greater than the additive effect. The non-allelic gene interaction was not significant. Considering the results mentioned above, it was regarded that there were two kinds of Significantly different genetic systems in the days to heading. 2. The rate of heterosis was significantly different depending upon the parents used in the crosses. For instance, the $F_1$'s from Togil cross showed high rate of heterosis in longer culm. Compared to short culm, longer culm was due to recesive gene action and short culm was due to recesive gene action. The dominant gene effect was greater than the additive gene effect in culm length. The narrow sense of heretability was very low and the maternal effects as well as reciprocal effects were significantly recognized. 3. The lenght of the of the uppermost internode of each $F_1$ plant was a little lorger than these of respective parental means or same as those of parents having long internodes, indicating partial dominance in the direction of lengthening the uppermost internodes. The additive gene effects on the uppermost internode was greater than the dominance gene effect. The narrow as well as broad sense of heritabilities for the character of the uppermost internode were very high. There were significant maternal and reciprocal effect in the uppermost internode. 4. The gene action for the flag leaf angle was rather dominance in a way of getting narrower angle. However, in the Palgueng combinations, heterosis of $F_1$ was observed in both narrow and wide angles of the flag leaf. The dominant effects were greater than the additive effects on the flag leaf angle. There were observed also a great deal of non-allelic gene interacticn on the inheritance of the flag leaf angle. 5. Even though the dominant gene action on the length and width of flag leaf was effective in increasing the length or width of the flag leaf, there were found various degrees of hetercsis depending upon the cross combination. Over-dominant gene effect were observed in the inheritance of length of the flag leaf, while additive gene effects was found in the inheritance of the width of the flag leaf. High degree of heretabilities, either narrow or broad sense, were found in both length and width of the flag leaf. No maternal and reciprocal effect were found in both characters. 6. When Tongil was used as one parent in the cross, the length of panicle of $F_1$'s was remarkedly longer than that of parents. In other cross comination, the length of panicle of $F_1$'s was close to the parental mean values. Rather greater dominent gene effect than additive gene effect was observed in the inheritance of panicle length and the dominant gene was effective in increasing the panicle length. 7. The effect of dominant genes was effective in increasing the number of panicles. The degree of heterosis was largely dependent on the cross combination. The effect of dominant gene in the inheritance of panicle number was a little greater than that of additive genes, and the inheritance of panicle number was assumed to be due to complete dominant gene effects. Significantly high maternal and reciprocal effects were found in the character studied. 8. There were minus and plus values of heterosis in the kernel number per panicle depending upon the cross combination. The mean dominant effect was effective in increasing the kernel number per panicle, the degree of dominant effect varied with cross combination. The dominant gene effect and non-allelic gene interaction were found in the inheritance of the kernel number per panicle. 9. Genetic studies were impossible for the maturing ratio, because of environmental effects such as hazards delaying heads. The dominant gene effect was responsible for improving the maturing ratio in all the cross combinations excluding Tongil 10. The heavier 1000 grain weight was due to dominant gene effects. The additive gene effects were greater than the dominant gene effect in the 1000 grain weight, indicating that partial dominance was responsible for increasing the 1000 grain weight. The heritabilites, either narrow or broad sense of, were high for the grain weight and maternal or reciprocal effects were not recognized. 11. When Tongil was used as parent, the straw weight was showing high heterosis in the direction of increasing the weight. But in other crosses, the straw weight of $F_1$'s was lower than those of parental mean values. The direction of dominant gene effect was plus or minus depending upon the cross combinations. The degree of dominance was also depending on the cross combination, and apparently high nonallelic gene interaction was observed.

• Journal of the Korean Wood Science and Technology
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• v.36 no.1
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• pp.69-78
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• 2008

Shearing strength test in tension type was investigated to determine the shear resistance of bolt and drift-pin connection of domestic larix glulam. The specimen was connected with bolt and drift-pin in the inserted plate type, and only bolt in the side plate type. The diameter of bolt and drift-pin used in the experiment are 12, 16 and 20 mm. The hole of bolt was drilled at the end-distance 5 d and 7 d. Tension load was loaded in the direction parallel to grain. The shear resistance was evaluated according to end-distance through this, the yield load was compared with the experimental yield load, using Larsen's formula. The prototype design strength is based on the yield load of end-distance 7 d and the reduction factor of end-distance 5 d was calculated. The results were as follows. 1. The average of maximum load of drift-pin connection was higher by 3~30% at the inserted type than at bolt connection with increasing diameter. In bolt connection, the average of maximum load of the side type was 1.54~2.07 times higher than that of the inserted type. In the same diameter, the average of maximum load of end-distance 7 d was higher by 8~44% than that of 5 d. 2. The bearing stress was 1.16~1.41 times higher at the inserted connection than at drift-pin connection, and 1.37~1.86 times higher at 7 d than at 5 d. Also, when the slenderness ratio was below 7.5 at drift-pin connection and below 6.0 at inserted connection, the lateral capacity was good. 3. The ratio of the experimental yield load and the predicted yield load calculated by Larsen's formula proposed by Larsen was 0.80~1.10 at inserted connection, and 0.75~1.46 at side connection. 4. When the inserted bolt connection was based on the yield load of end-distance 7 d, the reduction factor was 0.89 at 12 mm connection, 0.93 at 16 mm and 0.85 at 20 mm. The reduction factor was 0.89 at 12 mm the inserted drift-pin connection, 0.93 at 16 mm, 0.93 at 20 mm. The reduction factor was 0.79 at the side connection of the 12 mm bolt connection and 0.80 at 16 mm.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.4
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• pp.76-88
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• 2014

The purpose of this study is to derive landscape characteristic elements of agricultural and fishery heritage and to classify landscape characteristic elements of Cheongsando designated as Korean agricultural and fishery heritage No.1 as well as a Globally Important Agricultural Heritage System (GIAHS). Additionally, this study was conducted to suggest priority for conservation and management of Cheongsando by grasping differences between factors which visitors deem as important and satisfying. To implement this study, the literature review was written, an on-site survey to derive landscape characteristic elements of Cheongsando and a survey on importance and satisfaction of landscape characteristic elements of Cheongsando were conducted, and reliability analysis, descriptive statistical analysis and IPA analysis were performed using SPSS 20.0. The results are as follows: The analysis showed the factors requiring persistent efforts in the first quadrant are the rape flower garden, the sea, green barley field, flat stone paddy field, breakwater and lighthouse, abalone farms, stone houses, thatched houses, a coastal road, the slow road, Stonewall Walkway of Seongseo Village, and residents' agricultural behaviors. The analysis showed the factors needing intensive management strategies in the second quadrant are the surrounding mountain area, dock, Docheong Harbor, vessels, fish market, Doksari stone wall, garish-roofed farm villages, excursion school to a slow island, pension and cafe, bus stop, shade trees, Raw Fish Street, the beach and the filming site. Analysis indicated that the factors needing management control in the third quadrant are the pine grove, the beach, tidal mudflat, the garlic fields, vinyl greenhouses, grain drying yard, sea mustard drying yard, heritage center, Choboon, Dangri exorcism, the market place, residents' fishery behaviors, residents' industrial behaviors, residents' ordinary behaviors, visitors' behaviors that visiting the dock, visitors' behaviors that walking the slow road, visitors' behaviors that eating and shopping for specialties, visitors' behaviors that experiencing agriculture and fishery. Excessive effort factors in the fourth quadrant were not derived.