• 대한두개안면성형외과학회지
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• 제21권4호
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• pp.211-218
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• 2020

Autologous fat grafts are widely used in soft-tissue augmentation and reconstruction. To reduce the unpredictability of fat grafts and to improve their long-term survival, cell-assisted lipotransfer (CAL) was introduced. In this alternative method, autologous fat is mixed and grafted with stromal vascular fraction cells or adipose-derived stem/stromal cells (ASCs). In regenerative medicine, ASCs exhibit excellent therapeutic potential and are also simple to harvest. Although the efficacy of CAL has been demonstrated in experimental and clinical research, studies on its safety in terms of oncologic risk have reported inconclusive results. In order to establish CAL as a viable stem cell therapeutic approach, it will be necessary to demonstrate its oncologic safety in basic and clinical studies. Doing so could transform the paradigm of clinical strategy and practice for the treatment of a wide variety of diseases.

• Journal of Chest Surgery
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• 제45권4호
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• pp.251-253
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• 2012

We report a redo coronary artery bypass grafting (CABG) in a 55-year-old man. Angina recurred 7 years after the initial surgery. Coronary angiography showed all patent grafts except a faint visualization of the in situ right gastroepiploic artery (RGEA) graft, which was anastomosed to the posterior descending coronary artery, associated with celiac axis stenosis. Redo-CABG was performed at postoperative 10 years because of aggravated angina and decreased perfusion of the inferior wall in the myocardial single photon emission computed tomography. The saphenous vein graft was interposed between the 2 in situ grafts used previously; the right internal thoracic artery and RGEA grafts. Angina was relieved and myocardial perfusion was improved.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제18권1호
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• pp.164-174
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• 1996

In order to develop the allogeneic bone implants instead of autogenous bone grafts for maxillofacial reconstruction, undemineralized freeze-dried human bone was processed. The freeze-dried human bone was implanted into the cranial and mandibular defects of the rabbits. The implants were evaluated clinically, roentgenographically and histomophometrically. And immunohistochemical evaluation of the implants was performed on the rat. The results were as follows : 1. When compared with control defects of $0.8{\times}0.8\;cm$, the implants on the rabbit defects displayed complete osseous bridging clinically and roentgenographically. Histomophometrically a minimal inflammatory cell infiltrate was present but the defects healed well clinically. 2. When compared with control grafts, the freeze-dried implants on the rat muscle displayed decreased antigenicity by immunohistochemical evaluation, due to freeze-drying process. 3. Undemineralized freeze-dried human bone in this study can be preserved as a bank bone in this study and seems to be applicable for clinical allogeneic bone grafts.

• 대한두개안면성형외과학회지
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• 제25권2호
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• pp.99-103
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• 2024

Because facial nerve injuries affect the quality of life, leaving them untreated can have devastating effects. The number of patients with traumatic and iatrogenic facial nerve paralysis is considerably high. Early detection and prompt treatment during the acute injury phase are crucial, and immediate surgical treatment should be considered when complete facial nerve injury is suspected. Symptom underestimation by patients and clinical misdiagnosis may delay surgical intervention, which may negatively affect outcomes and in some cases, impair the recovery of the injured facial nerve. Here, we report two cases of facial nerve injury that were treated with nerve grafts during the subacute phase. In both cases, subacute facial nerve grafting achieved significant improvements. These cases highlight surgical intervention in the subacute phase using nerve grafts as an appropriate treatment for facial nerve injuries.

• Journal of Chest Surgery
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• 제39권6호
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• pp.427-433
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• 2006

배경: 현재까지 개발된 인조혈관들은 소구경 혈관에서는 개통성을 유지하기 어려워 사용에 제한이 따른다. 이에 저자는 이종혈관이식편이 소구경 혈관을 대신할 수 있는지 시험하고자 돼지의 혈관을 채취하여 냉동보관과 무세포화의 두 가지 방법으로 전처치한 뒤 이를 염소에게 이식하고 일정기간 동안 그 변화를 비교 분석하였다. 대상 및 방법: 돼지의 양측 경동맥을 적출한 뒤 하나는 바로 조직 보관액에 담아 냉동 보관하였고 하나는 NaCl-SDS 용액을 이용하여 무세포화(acellularization)한 뒤 냉동 보관하였다. 동일한 염소의 양측 경동맥에 냉동보관만 했던 이식편과 무세포화한 이식편을 각각 삽입하였다. 3마리의 염소에게 이를 시행하였고 술 후 각각 1, 3, 6개월째에 이식편을 적출하였다. 관찰기간 동안 주기적으로 초음파검사를 시행하여 개통성 여부를 확인하였으며, 이식 편의 적출 후에는 육안소견 및 광학현미경 소견을 비교 분석하였다. 결과: 3마리 실험동물 모두 혈전색전증과 같은 별다른 문제없이 예정된 적출시기까지 생존하였다. 초음파검사상 모든 혈관이식편에서 관찰기간 동안 우수한 개통성을 보였다. 육안소견상 이식편의 내강에서는 혈전의 생성없이 매끈한 표면을 관찰할 수 있었다. 현미경 검사상 6개월째의 혈관이식편에서 세포가 재구성되고 있는 것을 확인할 수 있었다. 이식 후 초기였을 때 무세포화이식편보다 단순 냉동이식편에서 염증반응이 활발한 것으로 보였으나 유의한 거부반응의 증거는 관찰되지 않았다. 결론: 돼지-염소간의 이종이식모델에서 단순 냉동혈관이식편과 무세포화혈관이식편 사이에 이식 후 6개월까지는 임상적인 차이를 야기하지 않았다. 이는 동일한 모델의 혈관이식에 있어 무세포화의 과정을 생략할 수 있다는 가능성 때문에 고무적인 결과라 할 수 있으나, 관찰기간이 비교적 짧고, 실험동물의 수가 많지 않아 향후 추가적인 연구가 뒷받침되어야 하리라 생각된다.

• Archives of Reconstructive Microsurgery
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• 제6권1호
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• pp.9-28
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• 1997

Adequate vascularization is pivotally essential for a successful nerve graft. Theoretically, the immediate vascularization will inhibit fibroblast infiltration and stimulate nerve cell regeneration. In this study, histomorphological and electrophysiological studies were performed to determine if vascularized grafts are functionally superior. In rat model, a 4cm segment of the sciatic nerve was obtained and placed as a non vascularized graft on one side, and as a vascularized graft connected to the inferior gluteal vessels on the opposite side. To determine the compound action potential of the gastrocnemius muscle, electromyography was done after 2, 3 and 4 months. Histomorphologically, the distribution of myelinated nerve fibers and Schwann cell were evaluated after toluidine blue staining, The following resutls were obtained: 1. The electrophysiological studies showed no difference between the nonvascularized and vascularized grafts. 2. Two and three months after grafting, myelinated nerve fibers were more abundant in the vascularized proximal, middle and distal areas in all nerve fibers of varying diameters. 3. In the post-nonvascularized graft 2-month group, a few myelinated nerve fibers were present in the proximal and middle areas, but none distally. In the post-vascularized graft 2 month group, myelinated nerve fibers ranging $2-8{\mu}m$ were present in all three areas. 4. In the post-nonvascularized graft 3 month group, a few myelinated nerve fibers ranging in $2-6{\mu}m$ were present in all three areas, but in the post-vascularized graft 3 month group, many myelinated nerve fibers ranging in $2-10{\mu}m$ were present in all three areas. 5. In the post-graft 4-month group, more myelinated nerve fibers were present in all three areas of the vascularized grafts. However, nerve fibers of less than $2{\mu}m$ in diameter were more abundant in the non vascularized grafts. 6. Schwann cells were more abundant in the proximal, middle and distal areas of the post-vascularized 2, 3 and 4-month grafts. Based on these findings, the immediate restoration of circulation in vascularized nerve grafts allows for the increased number of surviving Schwann cells, rapid healing of the axon and myelin sheath changes which occur during Wallerian degeneration, and thus is able to stimulate a morphologically optimal regeneration.

• Journal of Chest Surgery
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• 제49권4호
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• pp.225-231
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• 2016

Throughout its 30-year history, the right gastroepiploic artery (GEA) has been useful for in situ grafts in coronary artery bypass grafting (CABG). The early graft patency rate is high, and the late patency rate has improved by using the skeletonized GEA graft and proper target selection, which involves having a target coronary artery with a tight >90% stenosis. Total arterial revascularization with the internal thoracic artery and GEA grafts is an option for achieving better outcomes from CABG procedures.

• Journal of Chest Surgery
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• 제55권4호
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• pp.288-292
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• 2022

Ex vivo lung perfusion (EVLP) is a technique that enables active metabolism of the lung by creating an environment similar to that inside the body, even though the explanted lungs are outside the body. The EVLP system enables the use of lung grafts that do not satisfy the acceptance criteria for lung transplantation (LTx) by making it possible to evaluate the function of the lung grafts and repair lungs in poor condition, thereby reducing the waiting time of patients requiring LTx and consequently mortality.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제12권1호
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• pp.171-179
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• 1990

This is a report on a case of delayed reconstruction after partial mandibularectomy for Ameloblastoma involving symphysis and bodies of the mandible by using of two large pieces of corticocancellous bone blocks obtained from both iliac crests. The authors failed immediate reconstruction with two kinds of Alloplasts(metal, methylmethacrylate) at two times of trial due to known infection and suggestive poor histocompatibility of materials aginst the host who has been suffered from long-term and active pulmonary tuberculosis. However we could get success late reconstruction of this problem-occured and curved area with two pieces of large corticocancellous blocks by using of miniplate osteosynthesis and biphasic external pin fixation. From our experience we are obtained some results as follows : 1. Alloplastic graft materials seemed to be dangerous to maintain successfuly in patient who has a long-term debilitating disease as a active pulmonary tuberculosis. 2. Biphasic external skeletal pin fixation appliance gave many advantages such as maintain functional position of the remained bodies and condyles of the mandible after removal of failed alloplasts and during control of inflammation of this area, assist supportive roles to fix and maintain bone grafts during healing, allow mandibular movement during healing and so offer to conduct normal functional stimuli to bone grafts during osteogenesis etc. 3. Bony union was successful between not only normal bones and grafted bones but also grafted bone pieces even though we used two pieces of large blocks of corticocancellous bone for graft. 4. Miniplate osteosynthesis was not affect any adverse effects to bone grafts but offered good role of fixation and maintenance for bone grafts.

• Journal of Korean Dental Science
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• 제3권1호
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• pp.25-31
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• 2010

This study sought to evaluate the effect of the type of grafts used in sinus lifting. A review of literature through MEDLINE search covering the period 1980 ~ 2006 was performed. After screening, this study was narrowed down to 2,452 patients receiving sinus lift grafts wherein 7,151 implants were placed. In this study, the types of grafts used in sinus augmentation were autogenous bone, allogenic bone, corticocancellous block bone, and various alloplastic materials. The success rate varied from 69% to 100% depending on the graft material type. The highest success rate was reported for the autogenous bone, with high success rates recorded for the most part in most studies.