• Title/Summary/Keyword: Graduate Program

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Potential Swimming Motility Variation by AcrR in Escherichia coliS

  • Kim, Ye Jin;Im, So Young;Lee, Jae Ok;Kim, Ok Bin
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1824-1828
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    • 2016
  • AcrR, the toxic-compounds-response regulator, regulates motility in microorganisms, presumably to escape from toxic environments. In this study, the genome-wide target genes of AcrR were investigated in a ΔacrR mutant strain by microarray analysis. In the absence of AcrR, the transcription of most flagella/motility genes was highly increased. In addition, flagella formation was increased in this mutant strain. Motility assays revealed that AcrR modulates swimming motility, but not swarming.

A Measurement System for Rounded Shoulder Posture using a Wearable Stretch Sensor

  • Nguyen, Manh Thang;Dang, Quoc Khanh;Kim, Younghoon;Chee, Youngjoon
    • Journal of Biomedical Engineering Research
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    • v.39 no.1
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    • pp.10-15
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    • 2018
  • In this paper, we present a wearable measurement system for monitoring rounded shoulders. The system contains a shoulder correction band and a stretch sensor that can correct and measure shoulder posture, respectively. The capacitance of the stretch sensor changes linearly according to changes in the shoulders. To verify measurement, a motion analysis system was used as the reference to compare the change in the rounded angles of the shoulders and the change in the stretch sensor's capacitance. The results indicated that there is a high correlation between the two changes and the system can be used as a monitoring device for rounded shoulders.

Anti-fuse program circuits for configuration of the programmable logic device

  • Kim, Phil-Jung;Gu, Dae-Sung;Jung, Rae-Sung;Park, Hyun-Yong;Kim, Jong-Bin
    • Proceedings of the IEEK Conference
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    • 2002.07b
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    • pp.778-781
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    • 2002
  • In this paper, we designed the anti-fuse program circuit, and there are an anti-fuse program/sense/latch circuit, a negative voltage generator, power-up circuit and etc. in this circuit. An output voltage of a negative voltage generator is about -4,51V. We detected certainly it regardless of simulation result power rise time or temperature change to detect the anti-fuse program state of an anti-fuse program/sense/latch circuit and were able to know what performed a steady action. And as a result of having done a simulation while will change a resistance value voluntarily in order to check an anti-fuse resistance characteristic of this circuit oneself, it recognized as a programmed anti-fuse until 23k$\Omega$, and we were able to know that this circuit was a lot of margin than general anti-fuse resistance 500$\Omega$. Therefore, the anti-fuse program circuit of this study showed that was able to apply for configuration of the programmable logic device.

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Determination of Aflatoxin B1 in Rice, Barley, and Feed by Non-instrumental Immunochromatographic Strip-test and High Sensitive ELISA

  • Shim, Won-Bo;Kim, Jung-Sook;Kim, Ji-Young;Choi, Jin-Gil;Je, Jung-Hyun;Kuzmina, Nina Sergeevna;Eremin, Sergei Alexandrovich;Chung, Duck-Hwa
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.623-630
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    • 2008
  • A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples

  • Shim, Won-Bo;Yang, Zheng-You;Kim, Jung-Sook;Kim, Ji-Young;Kang, Sung-Jo;Woo, Gun-Jo;Chung, Young-Chul;Eremin, Sergei A.;Chung, Duck-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.17 no.10
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    • pp.1629-1637
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    • 2007
  • An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

Bacillus licheniformis Isolated from Korean Traditional Food Sources Enhances the Resistance of Caenorhabditis elegans to Infection by Staphylococcus aureus

  • Yun, Hyun Sun;Heo, Ju Hee;Son, Seok Jun;Park, Mi Ri;Oh, Sangnam;Song, Min-Ho;Kim, Jong Nam;Go, Gwang-Woong;Cho, Ho-Seong;Choi, Nag-Jin;Jo, Seung-Wha;Jeong, Do-Youn;Kim, Younghoon
    • Journal of Microbiology and Biotechnology
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    • v.24 no.8
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    • pp.1105-1108
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    • 2014
  • We investigated whether Bacillus spp., newly isolated from Korean traditional food resources, influence the resistance of hosts to foodborne pathogens, by using Caenorhabditis elegans as a surrogate host model. Initially, we selected 20 Bacillus spp. that possess antimicrobial activity against various foodborne pathogens, including Staphylococcus aureus. Among the selected strains, six strains of Bacillus spp. used in preconditioning significantly prolonged the survival of nematodes exposed to S. aureus. Based on 16S rRNA gene sequencing, all six strains were identified as B. licheniformis. Our findings suggest that preconditioning with B. licheniformis may modulate the host defense response against S. aureus.

Association Between Local Government Social Expenditures and Mortality Levels in Korea

  • Ko, Hansoo;Kim, Jinseob;Kim, Donggil;Kim, Saerom;Park, Yukyung;Kim, Chang-Yup
    • Journal of Preventive Medicine and Public Health
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    • v.46 no.1
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    • pp.1-9
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    • 2013
  • Objectives: We examined the association between social expenditures of the local government and the mortality level in Korea, 2004 to 2010. Methods: We used social expenditure data of 230 local governments during 2004 to 2010 from the Social Expenditure Database prepared by the Korean Institute for Health and Social Affairs. Fixed effect panel data regression analysis was adopted to look for associations between social expenditures and age-standardized mortality and the premature death index. Results: Social expenditures of local governments per capita was not significantly associated with standardized mortality but was associated with the premature death index (decline of 1.0 [for males] and 0.5 [for females] for each expenditure of 100 000 Korean won, i.e., approximately 100 US dollar). As an index of the voluntary effort of local governments, the self-managed project ratio was associated with a decline in the standardized mortality in females (decline of 0.4 for each increase of 1%). The share of health care was not significant. Conclusions: There were associations between social expenditures of the local government and the mortality level in Korea. In particular, social expenditures per capita were significantly associated with a decline in premature death. However, the voluntary efforts of local governments were not significantly related to the decline in premature death.

Kir4.1 is coexpressed with stemness markers in activated astrocytes in the injured brain and a Kir4.1 inhibitor BaCl2 negatively regulates neurosphere formation in culture

  • Kwon, Jae-Kyung;Choi, Dong-Joo;Yang, Haijie;Ko, Dong Wan;Jou, Ilo;Park, Sang Myun;Joe, Eun-Hye
    • The Korean Journal of Physiology and Pharmacology
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    • v.25 no.6
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    • pp.565-574
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    • 2021
  • Astrocytes are activated in response to brain damage. Here, we found that expression of Kir4.1, a major potassium channel in astrocytes, is increased in activated astrocytes in the injured brain together with upregulation of the neural stem cell markers, Sox2 and Nestin. Expression of Kir4.1 was also increased together with that of Nestin and Sox2 in neurospheres formed from dissociated P7 mouse brains. Using the Kir4.1 blocker BaCl2 to determine whether Kir4.1 is involved in acquisition of stemness, we found that inhibition of Kir4.1 activity caused a concentration-dependent increase in sphere size and Sox2 levels, but had little effect on Nestin levels. Moreover, induction of differentiation of cultured neural stem cells by withdrawing epidermal growth factor and fibroblast growth factor from the culture medium caused a sharp initial increase in Kir4.1 expression followed by a decrease, whereas Sox2 and Nestin levels continuously decreased. Inhibition of Kir4.1 had no effect on expression levels of Sox2 or Nestin, or the astrocyte and neuron markers glial fibrillary acidic protein and β-tubulin III, respectively. Taken together, these results indicate that Kir4.1 may control gain of stemness but not differentiation of stem cells.

Effect of the type of resin cement on the fracture resistance of chairside CAD-CAM materials after aging

  • Laura Vitoria Rizzatto;Daniel Meneghetti;Marielle Di Domenico;Julia Cadorin Facenda;Katia Raquel Weber;Pedro Henrique Corazza;Marcia Borba
    • The Journal of Advanced Prosthodontics
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    • v.15 no.3
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    • pp.136-144
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    • 2023
  • PURPOSE. The study objective was to evaluate the influence of the type of resin cement on the flexural strength and load to fracture of two chairside CADCAM materials after aging. MATERIALS AND METHODS. A polymer-infiltrated ceramic network (PICN) and a nanoceramic resin (RNC) were used to produce the specimens. Two types of dual-cure resin cements, a self-adhesive and a universal, were investigated. Bilayer specimens were produced (n = 10) and aged for 6 months in a humid environment before the biaxial flexural strength test (σf). Bonded specimens were subjected to a mechanical aging protocol (50 N, 2 Hz, 37℃ water, 500,000 cycles) before the compressive load test (Lf). σf and Lf data were analyzed using two-way ANOVA and Tukey tests (α = .05). Chi-square test was used to analyze the relationship between failure mode and experimental group (α = .05). RESULTS. The type of resin cement and the interaction between factors had no effect on the σf and Lf of the specimens, while the type of restorative material was significant. RNC had higher σf and Lf than PICN. There was a significant association among the type of cracks identified for specimens tested in Lf and the restorative material. CONCLUSION. The type of resin cement had no effect on the flexural strength and load to fracture of the two investigated CAD-CAM chairside materials after aging.

The Impact of Social Media Marketing Towards Purchase Decision: Interactive Flat Panel Display Technology Distribution from Indonesia's B2B Market

  • Yunita SWASTI;Ricardo INDRA;Nadia Kris SIGIT;Muhammad ILHAM;La MANI;Muhammad ARAS
    • Journal of Distribution Science
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    • v.22 no.9
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    • pp.129-139
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    • 2024
  • Purpose: This research aims to examine the impact of social media marketing on buy decisions in Indonesia's B2B market, considering the mediating roles of brand image, perceived quality, and perceived value in relation to interactive flat panel display technology. To better understand technology adoption and distribution, we utilize the innovation diffusion theory. Research Design, Data and Methodology: The Decision-Making Unit of each organization that buy interactive flat panel display technology conducted an empirical survey of 82 participants. The quantitative research design analyzed the data utilizing the PLS-SEM model. outcome: This research reveals that social media marketing significantly impacted perceived quality, brand image, perceived value, and buy decisions. The research also found that perceived quality does not significantly impact buy decisions, but perceived value and brand image significantly impacted buy decisions. This research contributes to understanding the key factorsinfluencing buy decisionsin Indonesia's B2B market. Conclusion: Thisresearch concludesthat B2B consumers in Indonesia are less concerned about product quality but prioritize the value they receive when purchasing interactive display technology. Social media marketing could impacted the distribution of interactive display technology in Indonesia's B2B market by affecting the DMU's purchasing decisions. Brandsshould leverage social media marketing to positively impact theirsuccess.