• 제목/요약/키워드: Gracilaria

검색결과 75건 처리시간 0.027초

꼬시래기 홍조류로부터 열산가수분해, 효소당화 및 에탄올 발효 (Thermal Acid Hydrolysis Pretreatment, Enzymatic Saccharification and Ethanol Fermentation from Red Seaweed, Gracilaria verrucosa)

  • 라채훈;최진규;강창한;선우인영;정귀택;김성구
    • 한국미생물·생명공학회지
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    • 제43권1호
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    • pp.9-15
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    • 2015
  • 본 연구는 해조류, 꼬시래기를 발효하여 에탄올을 생산하였다. 최적 전처리 조건은 12% (w/v) 해조류 슬러리, 270 mM 황산, 121도 60분동안 실시하였다. 열산가수분해 후에, 꼬시래기 가수분해산물에 16 U/ml의 혼합효소 Viscozyme L과 Celluclast 1.5 L를 이용하여 효소당화를 수행하였다. 50.4 g/l의총단당류의농도는, 120 g dw/l 꼬시래기 슬러리로부터 열산가수분해와효소당화에 의해 총 탄수화물 60 g/l의 전환율 84.2%를 나타내었다. 꼬시래기 가수분해산물은 분리당화발효(SHF)로 에탄올 생산을 위한 기질로 사용하였다. 고농도 galactose로 순치한 Candida lusitaniae ATCC42720에 의한 에탄올 생산은 0.43의 에탄올 수율(YEtOH)인 22.0 g/l를 생산하였다. 특정 당에 순치한 효모는 혼합당의 흡수에 유용하며, 그 결과 해조류가수분해산물배지로부터 높은 에탄올 수율을 나타내었다.

심근 경색 유발 심부전 모델에서 강리 추출물의 심장 보호 가능성 (Cardioprotective Potential of Gracilaria Verrucosa Extract in Myocardial Infarction-Induced Heart Failure Model)

  • 장윤재;김혜윰;윤정주;한병혁;유제국;조남근;이호섭;강대길
    • 대한한의학방제학회지
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    • 제31권3호
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    • pp.157-169
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    • 2023
  • Gracilaria Verrucosa (GV), a seaweed used in traditional Korean medicine, was studied for its effects on MI-induced heart failure in rats. MI is caused by a blocked coronary artery, leading to severe cardiac dysfunction. The study used a rat model to assess cardiac changes over time and evaluate the impact of GV on heart failure. Ischemia was induced through LAD ligation surgery, and the extent of ischemic area was measured as a prognostic factor. GV extract administration significantly improved cardiac morphology and reduced cardiac weight compared to the MI group. GV treatment also improved cardiac function, as evidenced by positive effects on chamber dilation during MI-induced heart failure. Parameters such as ejection fraction (EF) and fractional shortening (FS) were measured. The MI group showed decreased EF and FS compared to the sham group, while these parameters improved in the GV group. GV treatment also reduced levels of LDH, CPK, and CK-MB in the serum, indicating reduced myocardial damage. Histological analysis revealed that GV treatment attenuated cardiac hypertrophy and fibrosis, with reduced collagen deposition in the myocardium. Immunohistochemistry analysis showed suppressed expression of TGF-β1 and collagen 1, involved in fibrosis. In conclusion, GV showed potential in improving cardiac function in a rat model of MI-induced heart failure. It alleviated myocardial damage, attenuated cardiac hypertrophy and fibrosis, and suppressed fibrotic markers. Further studies are needed to explore its clinical efficacy and underlying mechanisms in cardiac diseases beyond animal models.

Reduction of Stress Caused by Drought and Salt in Rice (Oryza sativa L.) Crops through Applications of Selected Plant Extracts and the Physiological Response Mechanisms of Rice

  • Hyun Hwa Park;Young Seon Lee;Yong In Kuk
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.57-57
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    • 2022
  • In many areas of the world, salt damage and drought have had a negative impact on human survival due to a decrease in agricultural productivity. For instance, about 50% of agricultural land will be affected by salt damage by 2050. Biostimulants such as plant extracts can not only increase the nutrient utilization efficiency of plants, but also promote plant growth and increase resistance to abiotic or biotic stress. Therefore, the objective of this study was to determine how selected plant extracts might reduce levels of stress caused by drought and salt and to better understand the physiological response mechanisms of rice plants. In this study, we used Soybean leaves, Soybean stems and Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts were used. These extracts had been used in previous studies and were found to be effective. The materials were dried in a dry oven at 50℃ for 5 days and ground using a blender. Each 50 g of materials was put in 1 L of distilled water, stirred for 24 hours, filtered using 4 layers of mirocloth, and then concentrated using a concentrator. Rice (cv. Hopumbyeo) seeds were immersed and germinated, and then sown in seedbeds filled with commercial soil. In drought experiments, three rice seedlings at 1 week after seeding was transplanted into 100 ml cups filled with commercial soils and grown until the 4-leaf stage. For this experiment, the soil weight in a cup was equalized, and water was allowed to become 100% saturated and then drained for 24 hours. Thereafter, plant extracts at 3% concentrations were applied to the soils. For NaCl treatments, rice plants at 17 days after seeding were treated with either 100 mM NaCl or plant extracts at 1%+ 100 mM NaCl combinations in the growth chamber. Leaf injury, relative water content, photosynthetic efficiency, and chlorophyll contents were measured at 3, 5, and 6 days after treatments. Shoot fresh weight of rice under drought conditions increased 28-37% in response to treatments of Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Shoot fresh weight of rice subjected to 100 mM NaCl treatments also increased by 6-24% in response to Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Compared to the control, rice plants treated with these six extracts and subjected to drought conditions had significantly higher relative water content, Fv/Fm, total chlorophyll and total carotenoids than control plants. With the exception of relative water contents, rice plants treated with the six extracts and subjected to salt stress (100 mM NaCl treatments) had significantly higher Fv/Fm, total chlorophyll and total carotenoids than control plants. However, the type of extract used did not produce significant difference in these parameters. Thus, all the plant extracts used in this study could mitigate drought and NaCl stresses and could also contribute substantially to sustainable crop production.

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국내산 주요 해조류의 식이섬유소의 함량 및 추출조건 (Dietary Fiber Contents of Marine Algae and Extraction Condition of the Fiber)

  • 도정룡;김은미;구재근;조길석
    • 한국수산과학회지
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    • 제30권2호
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    • pp.291-296
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    • 1997
  • 국내 연안에서 생산되는 해조류 즉, 갈조류에 속하는 미역, 다시마 그리고 톳, 녹조류에 속하는 파래, 그리고 홍조류에 속하는 우뭇가사리, 꼬시래기에 함유된 식이섬유소의 함량과 이들 해조류로부터 가용성 식이섬유소를 효과적으로 추출하고자 산과 알칼리 그리고 효소를 사용하였다. 그 결과, 미역의 식이섬유 추출에는 $1.5\%$의 탄산나트륨 용액, 또는 $2.0\%$$Na_2EDTA$가 효과적인 것으로 나타났다. 다시마는 $1.0\%$$Na_2EDTA$, 톳은 $2.0\%$의 탄산나트륨, 우뭇가사리와 꼬시래기는 $2.0\%$$Na_2EDTA$를 사용하였을 때 가용성 식이섬유의 수율이 높았다. 파래는 탄산나트륨, 수산화나트륨 그리고 $Na_2EDTA$ 중에서 특별히 효과적인 것은 없었으며 $2\%$농도에서 $17.49\~18.46\%$로 나타났다. 또한 추출시간과 온도를 검토한 결과, $100^{\circ}C$ 에서 해조중의 식이섬유 추출은 추출시간이 길수록 추출율이 높게 나타났으며, 추출온도에도 큰 영향을 받는 것으로 나타나 우뭇가사리와 꼬시래기는 $110\~$에서 1시간 추출시 각각 $38.48\%$$42.11\%$의 높은 수율을 나타내었다.

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남해안 돌산도의 해조 II 조하대 해조군락의 구조 (Benthic Marine Algal of Dolsan-Island in the Southern Coast of Korea II Structure of Algal Communities of Subtidal Zone)

  • 손철현;이인규;강제원
    • 한국수산과학회지
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    • 제16권4호
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    • pp.379-388
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    • 1983
  • 본 조사는 전남 여천군 돌산읍 죽포리 두문포의 조하대 해조군락의 특성을 밝히기 위하여 1982년 6월 부터 1983년 5월 까지 연간 계절별로 SCUBA 잠수에 의해 채집된 자료를 분석하므로 수행되었다. 조하대의 수직분포는 sheltered 지역의 특성을 나타내고 있고, 상부, 중부, 하부의 3개 구역으로 나누어지며, 상부는 Ulva pertusa, Codium fragile, Chondria crassicaulis, Gigartina tenera의 녹조, 홍조류, 중부는 Myagropsis myagroides와 Sargassum tortile의 대형갈조류, 하부는 Plocamium telfairiae, Callophyllis japonica, Symphyocladia linearis의 홍조류가 우점종으로 나타났다. Normal association analysis를 통한 군락분석결과 이 지역 해조군락은 9개의 group으로 나누어지고, 각 group의 우점종은 각각 Sargassum tortile, Myagropsis myagroides, Chondrin crassicaulis, Codium fragile, Pterocladia tenuis, Gigartina tenera, Gracilaria textorii등이었고 이 지역의 해중림을 조성하는 대표적 종은 Myagropsis myagroides와 Sargassum tortile 이었다. 한편, Myagropsis myagroides의 현존량은 $50{\times}50cm$당 봄철에 5,248g, 여름에는 650.4g, 가을에는747.6g이었고, 평균체장은 봄부터 가을까지 계절별로 각각 169.9cm, 48,4cm, 149.9cm여서 생식이 끝난 직후인 봄철에 최고치를 나타내고 소멸기인 여름에 최소치를 나타냈다.

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Investigation of ${\alpha}$-Glucosidase Inhibitory Activity of Ethanolic Extracts from 19 Species of Marine Macroalgae in Korea

  • Jeong, So-Young;Qian, Zhong-Ji;Jin, Yeong-Jun;Kim, Gi-Ok;Yun, Pil-Yong;Cho, Tae-Oh
    • Natural Product Sciences
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    • 제18권2호
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    • pp.130-136
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    • 2012
  • In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) f rom all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on ${\alpha}$-glucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity ($IC_{50}$, 2.17 ${\mu}g/ml$) against ${\alpha}$-glucosidase, followed by Sargassum fulvellum (<$IC_{50}$, 8.13 ${\mu}g/ml$), Dictyopteris prolifera ($IC_{50}$, 16.66 ${\mu}g/ml$), Rugulopteryx okamurae ($IC_{50}$, 50.63 ${\mu}g/ml$), and Petrospongium rugosum ($IC_{50}$, 101.62 ${\mu}g/ml$). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.

서해 중부 연안 해조군집의 종조성과 생물량 (Species Composition and Biomass of Marine Algal Community on the Mid-Western Coast of Korea)

  • 김영환
    • Journal of Plant Biology
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    • 제38권4호
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    • pp.389-398
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    • 1995
  • 한국 서해 중부 연안의 12개 지소에서 조간대 해조류의 종조성과 생물량을 조사하였다. 조사지역에서 채집 동정된 해조류는 남조식물 19종, 녹조식물 20종, 갈조식물 32종 및 홍조식물 80종으로 총 151종이었다. 이 가운데 지충이(Sargassum thunbergii), 마디털(Stylonema alsidii) 및 꼬시래기(Gracilaria verrucosa)의 3종이 12개 조사지소 모두에서 출현하였다. 단위면적당 해조류 생물량은 백사장과 대천에서 41 g dry $wt{\cdot}m^{-2}$의 매우 낮은 평균 생물량을 보인 반면, 조사지역 가운데 가장 북쪽에 위치한 의항리에서 549 g dry $wt{\cdot}m^{-2}$로 가장 많았는데, 이 수준은 지금까지 서해안에서 측정된 해조류 생물량 가운데 가장 많은 양이다. 해조류의 문별 생물량 구성비율은 전반적으로 갈조식물이 가장 높게 나타난 반면, 녹조식물이 가장 낮았다. 출현종 가운데 지충이는 모든 조사지소에서 조간대 전반에 걸쳐 우점적인 생육을 보였다. 그러나 산호말류(Corallina spp.)는 조사지역의 상부인 태안반도의 지소들에서 우점적인 생육을 보였고 하부의 지소들에서도 부분적으로 출현하였으나, 중부의 지소들에서는 거의 출현하지 않는 분리 분포를 보였다.

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Assessment of the potential of algae phycobiliprotein nanoliposome for extending the shelf life of common carp burgers during refrigerated storage

  • Haghdoost, Amir;Golestan, Leila;Hasani, Maryam;Noghabi, Mostafa Shahidi;Shahidi, Seyed Ahmad
    • Fisheries and Aquatic Sciences
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    • 제25권5호
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    • pp.276-286
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    • 2022
  • This study is focused on the effect of phycobiliprotein extraction of Gracilaria on the quality of common carp burgers, and the application of nanoliposomes containing pigment in the improvement of its antimicrobial and antioxidant activity of burgers during refrigerated storage in 18 days. Burgers were incorporated with phycobiliprotein and liposomal phycobiliprotein (2.5% and 5% w/w), and their chemical and microbial changes in terms of pH, peroxide value (PV), thiobarbituric acid (TBA), total volatile basic nitrogen (TVB-N), total viable counts (TVC), psychrotrophic bacterial counts (PTC), and sensory characteristics were evaluated. Results presented a nanoliposome size of about 515.5 nm with capable encapsulation efficiency (83.98%). Our results showed non-encapsulated phycobiliprotein could delay the deterioration of common carp burgers, as a reduction in PV, TBA, and TVB-N, TVC, and PTC values in burgers treated with free and nano encapsulated phycobiliprotein. Moreover, the potential of phycobiliprotein was improved when it was encapsulated into chitosan coated liposomes. Burgers treated with 5% nanoliposomes displayed the lowest amount of lipid oxidation and microbial deterioration in comparison to others during storage. According to chemical, microbial and sensory evaluation, the shelf life of common carp burgers was increased in samples treated with encapsulated phycobiliprotein at 2.5% and 5%, as compared to the control (p ≤ 0.05).

부산 영도의 해조상과 곰피(Ecklonia stolonifera Okamura) 군락 (Algal Flora and Ecklonia stolonifera Okamura (Laminariaceae) Population of Youngdo in Busan, Korea)

  • 최창근
    • ALGAE
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    • 제22권4호
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    • pp.313-318
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    • 2007
  • Studies have been made of the species composition and community structure of benthic marine algae at intertidal zone and subtidal zone of Youngdo at Busan. In this area, a total of 69 marine algal species including 10 Chlorophyta, 17 Phaeophyta and 42 Rhodophyta was identified. Dominant species in coverage were Enteromorpha linza, Ulva pertusa, Gelidium divaricatum, Hildenbrandtia rubra and Chondrus ocellatus throughout the year. Enteromorpha linza, Ulva pertusa, Porphyra yezoensis, Gelidium divaricatum, Corallina pilulifera, Grateloupia elliptica and Chondrus ocellatus vertically distributed in intertidal zone, while Ecklonia stolonifera, Gelidium amansii, Prionitis cornea, Gracilaria textorii, Acrosorium polyneurum and Phycodrys fimbriata in subtidal zone. The vertical distribution of subtidal zone were characterized by Ecklonia stolonifera at 1 to 5 m depths. During a year, biomass and length of Ecklonia stolonifera gradually decreased from March to January, but increased from February. Zoosporangial sori were observed from September to December. In conclusion, number of species in this area was remarkably reduced as compared to the previous data at Busan and it’s vicinity.

북극 스발바드 군도 Kongsfjorden의 해조상 (Macroalgal Flora of Kongsfjorden in Svalbard Islands, the Arctic)

  • 김지희;정호성;최한구;김예동
    • Ocean and Polar Research
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    • 제25권4호
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    • pp.569-591
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    • 2003
  • Marine benthic flora was investigated in an Arctic bay. Specimens of chlorophyte, phaeophyte, and rhodophyte were collected and examined over the period from July to August 2003 from Kongsfjorden Spitsbergen in Svalbard Islands. A total of 28 genera and 32 species (5 chlorophytes, 18 phaeophytes, and 9 rhodophytes) was identified and described. A green alga Enteromorpha linza(Linnaeus) J. Agardh, a brown alga Asperococcus compresus Griffiths ex Hooker, and three red algae Gracilaria gracilis (Stackhouse) Steentoft et al., Rhodymenia pacifica Kylin and Schizochlaenion rhodotrichum Wynne et Norris were recorded in Svalbard Islands for the first time.