• 제목/요약/키워드: Good Laboratory Practice

검색결과 104건 처리시간 0.02초

PFOS salts 및 PFOS 대체물질에 대한 미생물분해시험 (Biodegradation test of the alternatives of perfluorooctanesulfonate (PFOS) and PFOS salts)

  • 최봉인;나숙현;손준효;신동수;유병택;정선용
    • 한국환경보건학회지
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    • 제42권2호
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    • pp.112-117
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    • 2016
  • Objectives: In this study, we investigated the biodegradation rates of 8 perfluorooctanesulfonate (PFOS) alternatives synthesized at the at Changwon National University in comparison to those of PFOS potassium salt and PFOS sodium salt. Methods: A biodegradability test was performed for 28 days with microorganisms cultured in the good laboratory practice laboratory at the Korea Environment Corporation following the OECD Guidelines for the testing of chemicals, Test No. 301 C Results: While $C_5H_8F_3SO_3K$, $C_8F_{17}SO_3K$ and $C_8F_{17}SO_3Na$ were not degraded after 28 days, the 3 alternatives were biodegraded at the rates of 31.4% for $C_8H_8F_9SO_3K$, 25.6% for $C_{10}H_8F_{13}SO_3K$, 23.6% for $C_{25}F_{17}H_{32}S_3O_{13}Na_3$, 20.9% for $C_{15}F_9H_{21}S_2O_8Na_2$, 15.5% for $C_{23}F_{18}H_{28}S_2O_8Na_2$, 8.5% for $C_{17}F_9H_{25}S_2O_8Na_2$ and 4.8% for $C_6H_8F_5SO_3K$. When the concentration was the same(500 mg/L), $C_{23}F_{18}H_{28}S_2O_8Na_2$ had the lowest tension with 20.94 mN/m, which was followed by $C_{15}F_9H_{21}S_2O_8Na_2$ (23.36 mN/m), $C_{17}F_9H_{25}S_2O_8Na_2$ (27.31 mN/m), $C_{25}F_{17}H_{32}S_3O_{13}Na_3$ (28.17 mN/m), $C_{10}H_8F_{13}SO_3K$ (29.77 mN/m) and $C_8H_8F_9SO_3K$ (33.89 mN/m). Having higher surface tension of 57.64 mN/m and 67.57 mN/m, respectively, than those of the two types of PFOS salts, $C_6H_8F_5SO_3K$ and $C_5H_8F_3SO_3K$ were found valueless as substitute for PFOS. Conclusion: The biodegradation test suggest that 6 compounds could be used as substitutes for PFOS. $C_{23}F_{18}H_{28}S_2O_8Na_2$ and $C_{15}F_9H_{21}S_2O_8Na_2$ were found to be the best substitutes based on biodegradation rate and surface tension, followed by $C_{25}F_{17}H_{32}S_3O_{13}Na_3$, $C_8H_8F_9SO_3K$ and $C_{10}H_8F_{13}SO_3K$. $C_{17}F_9H_{25}S_2O_8Na_2$ was found to have relatively low value as an alternative but it still had a potential to substitute the conventional PFOS.

연속체 암반비탈면의 강도정수 산정 연구 (A Study on Estimating Shear Strength of Continuum Rock Slope)

  • 김형민;이수곤;이벽규;우재경;허익;이준기
    • 한국지반공학회논문집
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    • 제35권5호
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    • pp.5-19
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    • 2019
  • 암반으로 구성되어 있는 급경사($65^{\circ}{\sim}85^{\circ}$)비탈면이 장기간 안정한 상태로 유지되고 있는 자연현상을 고려할 때, 설계 및 초기 시공 단계에서 위와 유사한 지반 상태로 이루어진 깎기 암반비탈면에 대해 1:0.5(발파암 경사 기준)보다 급한 경사를 적용할 수 있을 것이다. 급경사로 설계 가능한 양호한 연속체 암반비탈면의 안정성을 검토하는 과정에서, 설계실무 측면에서 범용적인 암반강도정수 산정방법이 필요하게 되었다. Hoek 등(2002)이 수정 보완하여 발표한 Hoek-Brown 파괴기준과 GSI분류는 불연속구조의 영향을 충분히 고려한 암반특성화 시스템으로 평가되었으며, 응력변화에 따라 등가 Mohr-Coulomb 강도정수(등면적법)를 산출하는 방법을 제안하였다. 비탈면에서는 등가 M-C 강도정수가 최대구속응력(${{\sigma}^{\prime}}_{3max}$ 또는 수직응력)변화에 따라 민감하게 변화하므로 실무적으로 활용하기에 어려운 점이 있다. 이 연구에서는 양호한 연속체 암반비탈면에 대해 최대구속응력 범위이내에서 범용적으로 적용할 수 있는 강도정수산정방법(등각분할법)을 H-B 파괴기준을 응용하여 제안한다. 등각분할법 강도정수(A)의 타당성 및 적용성을 평가하기 위해, 연구대상 암반비탈면을 기존 실시설계 현장 인근에 있는 급경사 비탈면에서 암석종류별(화성암, 변성암, 퇴적암)로 선정하고, Hoek이 제시한 등가 M-C 강도정수(등면적법)들과 비교 분석하였다. 등면적법 및 등각분할법 등가 M-C 강도정수는 기본적인 자료인 기존 실시설계 현장의 실내 암석 삼축압축시험과 연구대상 암반비탈면의 불연속구조의 특성조사(Face Mapping)를 통해 RocLab 프로그램(H-B 파괴기준을 기본으로 전산화된 지반정수 산정 소프트웨어)을 활용하여 산정하였다. 산정된 등면적법 등가 M-C 강도정수는 상호 연동되어 점착력과 내부마찰각이 아주 크거나($45^{\circ}$ 이상) 작게 나타났다. 등각분할법 등가 M-C 강도정수는 등면적법 등가 M-C 강도정수의 중간 정도이며, 내부마찰각은 $30^{\circ}{\sim}42^{\circ}$의 범위를 보인다. 연구대상 암반비탈면의 등각분할법 강도정수(A)와 기존 실시 설계 현장에서 연구대상 암반비탈면과 유사한 암반상태(동일 등급 RMR)에 적용한 강도정수(B)와 비교 분석하고, 이 지반정수들로 적용한 비탈면 안정해석(한계평형해석과 유한요소해석) 결과를 통해 제안한 등각분할법의 적용성을 간접적으로 평가하였다. A와 B의 강도정수 차이는 10% 정도이다. 한계평형해석 결과(우기 기준), A적용 안전율(Fs)=14.08~58.22(평균 32.9), B적용 안전율(Fs)=18.39~60.04(평균 32.2)이며, 각 동일한 암석종류에 따라 상호 유사하게 나타났다. 유한요소 해석 결과, A적용 변위=0.13~0.65mm(평균 0.27mm), B적용 변위=0.14~1.07mm(평균 0.37mm)으로 매우 유사하다. H-B 파괴기준을 응용하여 등각분할법으로 산출한 지반 정수를 실무적인 전단강도로 적용할 수 있는 적용성 평가에서 유의미한 결과를 확인할 수 있었다.

한국농촌보건(韓國農村保健)의 문제점(問題點)과 개선방안(改善方案) (Innovative approaches to the health problems of rural Korea)

  • 노인규
    • 농촌의학ㆍ지역보건
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    • 제1권1호
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    • pp.5-9
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    • 1976
  • The categories of national health problems may be mainly divided into health promotion, problems of diseases, and population-economic problems which are indirectly related to health. Of them, the problems of diseases will be exclusively dealt with this speech. Rurality and Disease Problems There are many differences between rural and urban areas. In general, indicators of rurality are small size of towns, dispersion of the population, remoteness from urban centers, inadequacy of public transportation, poor communication, inadequate sanitation, poor housing, poverty, little education lack of health personnels and facilities, and in-accessibility to health services. The influence of such conditions creates, directly or indirectly, many problems of diseases in the rural areas. Those art the occurrence of preventable diseases, deterioration and prolongation of illness due to loss of chance to get early treatment, decreased or prolonged labour force loss, unnecessary death, doubling of medical cost, and economic loss. Some Considerations of Innovative Approach The followings art some considerations of innovative approaches to the problems of diseases in the rural Korea. 1. It would be essential goal of the innovative approaches that the damage and economic loss due to diseases will be maintained to minimum level by minimizing the absolute amount of the diseases, and by moderating the fee for medical cares. The goal of the minimization of the disease amount may be achieved by preventive services and early treatment, and the goal of moderating the medical fee may be achieved by lowering the prime cost and by adjusting the medical fees to reasonable level. 2. Community health service or community medicine will be adopted as a innovative means to disease problems. In this case, a community is defined as an unit area where supply and utilization of primary service activities can be accomplished within a day. The essential nature o the community health service should be such activities as health promotion, preventive measures, medical care, and rehabilitation performing efficiently through the organized efforts of the residents in a community. Each service activity should cover all members of the residents in a community in its plan and performance. The cooperation of the community peoples in one of the essential elements for success of the service program, The motivations of their cooperative mood may be activated through several ways: when the participation of the residents in service program of especially the direct participation of organized cooperation of the area leaders art achieved through a means of health education: when the residents get actual experience of having received the benefit of good quality services; and when the health personnels being armed with an idealism that they art working in the areas to help health problems of the residents, maintain good human relationships with them. For the success of a community health service program, a personnel who is in charge of leadership and has an able, a sincere and a steady characters seems to be required in a community. The government should lead and support the community health service programs of the nation under the basis of results appeared in the demonstrative programs so as to be carried out the programs efficiently. Moss of the health problems may be treated properly in the community levels through suitable community health service programs but there might be some problems which art beyond their abilities to be dealt with. To solve such problems each community health service program should be under the referral systems which are connected with health centers, hospitals, and so forth. 3. An approach should be intensively groped to have a physician in each community. The shortage of physicians in rural areas is world-wide problem and so is the Korean situation. In the past the government has initiated a system of area-limited physician, coercion, and a small scale of scholarship program with unsatisfactory results. But there might be ways of achieving the goal by intervice, broadened, and continuous approaches. There will be several ways of approach to motivate the physicians to be settled in a rural community. They are, for examples, to expos the students to the community health service programs during training, to be run community health service programs by every health or medical schools and other main medical facilities, communication activities and advertisement, desire of community peoples to invite a physician, scholarship program, payment of satisfactory level, fulfilment of military obligation in case of a future draft, economic growth and development of rural communities, sufficiency of health and medical facilities, provision of proper medical care system, coercion, and so forth. And, hopefully, more useful reference data on the motivations may be available when a survey be conducted to the physicians who are presently engaging in the rural community levels. 4. In communities where the availability of a physician is difficult, a trial to use physician extenders, under certain conditions, may be considered. The reason is that it would be beneficial for the health of the residents to give them the remedies of primary medical care through the extenders rather than to leave their medical problems out of management. The followings are the conditions to be considered when the physician extenders are used: their positions will be prescribed as a temporary one instead of permanent one so as to allow easy replacement of the position with a physician applicant; the extender will be under periodic direction and supervision of a physician, and also referral channel will be provided: legal constraints will be placed upon the extenders primary care practice, and the physician extenders will used only under the public medical care system. 5. For the balanced health care delivery, a greater investment to the rural areas is needed to compensate weak points of a rurality. The characteristics of a rurality has been already mentioned. The objective of balanced service for rural communities to level up that of urban areas will be hard to achieve without greater efforts and supports. For example, rural communities need mobile powers more than urban areas, communication network is extremely necessary at health delivery facilities in rural areas as well as the need of urban areas, health and medical facilities in rural areas should be provided more substantially than those of urban areas to minimize, in a sense, the amount of patient consultation and request of laboratory specimens through referral system of which procedures are more troublesome in rural areas, and more intensive control measures against communicable diseases are needed in rural areas where greater numbers of cases are occurred under the poor sanitary conditions.

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Sialic Acid를 지표성분으로 하는 유청가수분해단백분말의 기능성식품 개발연구 - I. 효소분리로 7% Siailc Acid가 표준적으로 함유된 유청가수분해단백분말(7%)의 랫드를 이용한 90일 반복경구투여 독성시험 평가 연구 - (Development and Research into Functional Foods from Hydrolyzed Whey Protein Powder with Sialic Acid as Its Index Component - I. Repeated 90-day Oral Administration Toxicity Test using Rats Administered Hydrolyzed Whey Protein Powder containing Normal Concentration of Sialic Acid (7%) with Enzyme Separation Method -)

  • 노혜지;조향현;김희경
    • Journal of Dairy Science and Biotechnology
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    • 제34권2호
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    • pp.99-116
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    • 2016
  • 본 시험은 sialic acid가 7%를 함유하도록 제조한 유청가수분해단백분말제제(whey protein of hydrolysis)의 기능성 식품원료로 개발을 위한 동물안전성을 평가함에 연구목표를 두었다. GMP를 원료로 제조한 시험물질은 sialic acid 7%(v/v)와 원료인 GMP 가수분해 단백질이 93%로 구성되어 있었다(시험명: 7%-GNANA). 시험물질의 독성 유무는 한국식품의약안전청(KFDA, 2014)과 OECD(2008)의 의약품 등의 독성시험 기준에 따라 실시하였다. 평가방법으로서, 시험물질의 투여용량을 0, 1,250, 2,500 및 5,000 mg/kg/day로 하여 SPF Sprague-Dawley 계열 암수 랫드에 90일 동안 반복경구투여하였을 때 나타나는 독성 여부를 평가하였다. 평가항목으로서는 사망률, 일반증상관찰, 체중 변화, 사료 섭취량 측정, 안검사, 요검사, 혈액학적 및 혈액생화학적 검사, 부검 시 장기의 중량측정, 부검 시 육안적 검사 및 조직병리학적 검사 등을 평가하였다. 90일 반복경구투여 실험결과로서, 시험물질투여 및 관찰기간 동안 사망동물은 발생하지 않았다. 또한 일반증상, 체중 변화, 사료섭취량, 안과학적 검사, 요검사 그리고 혈액학적 및 혈액이화학적 이상 및 혈액응고검사에서 대조군 대비 특이한 변화는 관찰되지 않았다(p<0.05). 부검 및 병리조직학적 평가 결과, 암수 모두에서 시험물질-유래 중요한 변화 없이 시험물질-유래 경미한 변화(non-adverse effect)만인 5,000 mg/kg/day에서 확인되었다. Weight-based classification(독성 강도에 따른 분류)을 적용한 최종 독성평가 결과, 수컷의 경우 NOEL(No Observed Effect Level)은 5,000 mg/kg/day 그리고 암컷의 경우는 NOAEL(No Observed Adverse Effect Level)은 5,000 mg/kg/day로 최종 확인되었다. 따라서, 암수 모두에서 시험물질의 NOAEL은 투여최대용량인 5,000 mg/kg/day로 확인되었다. 결론적으로, GMP를 원료로 하여 제조한 7%-GNANA(유청가수분해단백분말)는 투여가능 최대용량에서도 독성이 없는 안전한 천연물이라는 것을 확인하였고, 의약품이나 기능성 식품으로서의 개발 가능성을 확인하였다.