• 공업화학
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• 제32권3호
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• pp.312-319
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• 2021

광열 치료(photothermal therapy)란 빛을 조사하여 열을 발생시킴으로써 정상세포보다 열에 약한 비정상 세포, 특히 암세포를 선택적으로 괴사시키는 치료법이다. 본 연구에서는 광열 치료를 위한 카르복실화된 환원 그래핀옥사이드(reduced graphene oxide with carboxyl groups, CRGO)-골드나노막대(gold nanorod, AuNR) 나노복합체를 합성하고자 하였다. 이를 위해 그래핀옥사이드(graphene oxide, GO)를 고온에서 선택적으로 환원, 박리하여 CRGO를 합성하였고, AgNO₃의 양에 따라 AuNR의 길이를 조절하여 880 nm에서 강한 흡광 특성을 나타내는 AuNR를 합성하여 광열 인자로 사용하였다. 일반적인 방법으로 환원된 RGO에 비해 CRGO에 상대적으로 많은 카르복실기가 결합되어 있음을 FT-IR, 열 중량 분석 및 형광 분석을 통해 확인하였다. 또한, RGO에 비해 많은 carboxyl group이 결합된 CRGO는 수용액상에서 우수한 안정성을 나타내었다. 정전기적 상호작용을 통해 합성된 CRGO-AuNR 나노복합체는 약 317 nm의 균일한 크기와 좁은 크기 분포를 보였다. CRGO-AuNR 나노복합체는 두 가지 광열 인자인 CRGO와 AuNR의 synergistic effect로 인하여 조직 투과도가 우수한 근적외선 880 nm 레이저의 조사에 의한 광열 효과가 AuNR보다 2배 이상 향상 되는 것을 확인하였다. 또한, 광열 효과에 의한 암세포 독성 분석 결과, CRGO-AuNR 나노복합체가 가장 우수한 세포 독성 특성을 나타내었다. 따라서 CRGO-AuNR 나노복합체는 안정된 분산성과 향상된 광열 효과를 기반으로 항암 광열 요법 분야에 응용될 수 있을 것으로 기대된다.

• 산업기술연구
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• 제29권B호
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• pp.229-232
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• 2009

We prepared gold nanoparticles (Au NPs) by reduction-oxidation reaction between $HAuCl_4$ and trisodium citrate and measured the size and morphology of Au NPs by TEM for various molar ratios of $HAuCl_4$ to citrate and for various concentrations of $HAuCl_4$. UV-vis spectroscopy was used to characterize the optical properties of Au NPs. Au NPs in the size range from 14.3 nm to 20.3 nm were prepared with monodisperse distribution.

• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.3047-3051
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• 2014

In this paper, we describe a new method for the selective extraction and quantification of glutathione (GSH) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and maleimide-presenting gold nanoparticles (Mal-AuNPs). Our strategy utilizes the Michael addition to selectively extract GSH, from chosen samples, onto the maleimide of Mal-AuNPs. After the extraction step, the GSH bound to the AuNPs was analyzed by MALDI-TOF MS in the presence of an internal standard which was prepared by reacting Mal-AuNPs with isotope-labeled GSH ($GSH^*$). The $GSH^*$ has the same structure as GSH but a higher molecular weight, and therefore, enables absolute quantification of GSH by comparing the mass signal intensities of the GSH- and $GSH^*$-conjugated alkanethiols. Our strategy was verified by analyzing GSH-spiked fetal bovine serum and NIH 3T3 cells.

• Journal of the Korean Physical Society
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• 제73권11호
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• pp.1725-1728
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• 2018

We synthesized the gold nanoparticles (Au NPs)-embedded methylammonium lead iodide ($MAPbI_3$) film for the first time. The effects of metal nanoparticles on $MAPbI_3$ perovskite were systematically studied using UV-Vis absorption and photoluminescence (PL) measurements. As a result, the 20-nm-sized Au NPs-embedded $MAPbI_3$ film exhibited a 4.15% higher absorbance than the bare $MAPbI_3$ film. Moreover, the average PL intensity of the Au NPs-embedded $MAPbI_3$ film increased by about 75.25% over the bare $MAPbI_3$ film. Therefore, we have confirmed that addition of the Au NPs has a positive effect on the optical properties of $MAPbI_3$, and we believe that this study will provide a basic insight into the metal nanoparticles-embedded perovskite thin films for the future optoelectronic applications.

• Mass Spectrometry Letters
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• 제15권1호
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• pp.26-39
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• 2024

Gold nanostructures (Au NSs) are useful and interesting matrices for mass spectrometric analysis of various biomolecules based on organic matrix-free laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS). Au NSs provide high efficiency and versatility in LDI-TOF-MS analysis based on their well-established synthesis and surface functionalization, large surface area, high laser absorption capacity, and photothermal conversion efficiency. Therefore, Au NSs based LDI-TOF-MS can be a facile, functional, and efficient analytical method for important small biomolecules owing to its simple preparation, rapid analysis, salt-tolerance, signal reproducibility, and quantitative analysis. This review chronologically summarizes the important advance of Au NSs-based LDI-TOF-MS platforms in terms of in-depth mechanism, signal enhancement, quantitative analysis, and disease diagnosis.

• 접착 및 계면
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• 제11권4호
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• pp.144-148
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• 2010

본 연구에서는 플라즈몬 공명 신호 증폭을 위한 AuNPs의 실리카 표면 개질 및 나노 입자의 안정적인 표면 박막 형성을 목표로 하였다. 직경 10 nm의 AuNPs를 수용액 상에서 제조하였으며, AuNPs 표면에 실리카 층을 단계별로 형성시켰다. Tetraethlyorthosilicate 농도를 조절함으로써 실리카 박막 두께를 조절하였으며, 얻어진 나노 입자들을 수용액 표면에 분산시켜 Langmuir-Blodgett 박막을 제조하였다. 흡광스펙트럼의 변화를 관찰함으로써 AuNPs의 크기 변화를 확인하였으며, 원자 힘 현미경으로 LB 박막의 형성 여부와 표면 균일도를 살펴보았다.

• Clinical and Experimental Vaccine Research
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• 제12권1호
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• pp.32-46
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• 2023

Purpose: The present study aimed to compare the immune-enhancing potential of gold nanoparticles (AuNPs) to Alum against rabies vaccine and the related immunological, physiological, and histopathological effects. Materials and Methods: Alum and AuNPs sole and in combination with rabies vaccine were used at 0.35 mg/mL and 40 nM/mL, respectively. Rats used were categorized into six groups (20/each): control rats, rabies vaccine, aluminum phosphate gel, rabies vaccine adsorbed to Alum, AuNPs, and rabies vaccine adjuvant AuNPs. Results: Liver and kidney functions were in the normal range after AuNPs and Alum adjuvanted vaccine compared to control. Interleukin-6 and interferon-γ levels were significantly increased in groups immunized with Alum and AuNPs adjuvanted vaccine, the peak level was in the case of AuNP adjuvanted vaccine on the 14th day. Ninety days post-vaccination, total immunoglobulin G (IgG) against adjuvanted rabies vaccine showed a significantly elevated antirabies IgG with AuNPs and Alum adsorbed vaccine compared with unadjuvanted one. The total antioxidant capacity, malondialdehyde (MDA) levels, superoxide dismutase, and glutathione peroxidase activities were significantly increased post-adjuvanted AuNPs adjuvanted vaccine vaccination than in Alum adsorbed vaccine, while MDA was significantly decreased. The histopathological examination revealed detectable alterations post-AuNPs and Alum adjuvanted vaccine immunization compared with liver and kidney profiles post-administration of unadjuvanted and non-immunized groups, meanwhile, splenic tissue revealed hyperplasia of lymphoid follicles indicating increased immune reactivity. Conclusion: The AuNPs are promising enhancers of the immune response as Alum, and the undesirable effects of AuNPs could be managed by using suitable sizes, shapes, and concentrations.

• 대한수의학회지
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• 제63권4호
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• pp.33.1-33.5
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• 2023

Many scolicidal agents have been used to destroy fertile protoscolices, but these scolicidal agents have side effects, highlighting the need for research on effective and non-toxic replacement scolicidal agents. Gold nanoparticles (AuNPs) are biocompatible and non-toxic. The current study examined the effects of AuNPs in killing the protoscolices of Echinococcus granulosus in vitro using eosin staining. The protoscolices were treated with 0.2, 0.4, 0.8, or 1.0 mg/mL of AuNPs for 15, 30, 45, or 60 minutes. A concentration of 1.0 mg/mL was the most efficient in killing the protoscolices after 60 minutes exposure, reaching 96%, followed by 0.8 mg/mL (84.5%), whereas 0.4 and 0.2 mg/mL of AuNPs achieved a death rate of 76.8% and 68.5%, respectively. The loss of the protoscolices was lower at shorter exposure times with the same concentration of AuNPs and increased as the AuNP concentration was increased at the same exposure time. Significant differences were found between the different groups compared to the control group.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.421.1-421.1
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• 2014

Top-down processes based on photolithography technology have been developed by using light sources with short wavelength, however, the processes are expected to meet their limits in higher integration of semiconductor integrated circuits. To overcome the limits, researches on bottom-up processes have been proceeded. One of those, fabrication of nanodevices by using nanoparticles has been on research. But it is difficult to align nanoparticles at appropriate positions. To resolve this, studies has been proceeded to form nanowires by bonding DNA molecules which have self-assembly property and positive-charged functionalized gold nanoparticles. There are negative-charged phosphates in backbones of DNA molecules. By using the attractive force between the negative charge of the phosphates and the positive charge of gold nanoparticles, the Au-DNA nanowires are made. However, bonding Au nanoparticles only on DNA molecules, not other nanoparticles, is to be solved. So we studied to resolve this problem. In the formation of Au nanoparticles, we changed the charge of Au nanoparticles by adding HCl to control pH of the functionalized nanoparticles, measured zeta potential. Then we bonded the nanoparticles and DNA molecules and made observation by using FE-SEM and AFM.

• 한국산학기술학회논문지
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• 제18권4호
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• pp.608-613
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• 2017

본 연구에서는 저품위인 35wt% 금합금에 대해 80.0wt% 이상의 Au를 얻기 위한 건식 정련 공정을 제안하였다. Au35wt%-Ag5wt%-Cu60wt%의 조성을 가진 금합금에 대해 PbO/(PbO+CaO)의 혼합비를 각각 0~1로 변화시키고 플럭스/금합금의 무게비는 1/2로 하여 $1200^{\circ}C$-5시간의 열처리를 진행하였다. 이때 공정 전, 후 시료의 조성 변화는 energy dispersive X-ray spectroscopy(EDS)로 확인하고, 공정이 완료된 후 분리된 플럭스 금속 원소 성분은 time of flight secondary ion mass spectromerty(ToF-SIMS)로 확인하였다. EDS분석 결과 플럭스의 비율이 1(PbO 단일)인 경우 Au의 함량이 35.0wt%에서 86.7wt%로 가장 크게 향상되었고, 다른 플럭스 조성의 경우도 84wt% 이상으로 정련이 가능하였다. 또한 2/3 혼합비의 플럭스에서 Ag가 플럭스부로 빠져나가는 손실이 가장 적었다. 플럭스부의 ToF-SIMS 분석 결과 플럭스의 비율이 1, 0 일 때 $Au^+$의 특성 피크의 강도가 각각 349, 37로 측정되었다. Au의 손실을 고려하였을 때 CaO 단일 플럭스의 사용이 더 유리할 수 있었으나, 이 정도의 신호강도는 무시할 수 있는 정도로 판단되었다. 따라서 혼합플럭스를 이용한 건식 열처리를 통해 효과적인 금의 정련이 가능하여 경제적인 습식제련의 전처리 공정으로 사용될 수 있음을 확인하였다.