• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.63-68
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• 1994

432 samples of raw goat milk were analyzed for sodium, calcium, potassium, chloride, and ashes content. The samples came from 16 pure breed goats, 2 to 4 years of age kept in semi-feedlots. They were basically fed soy bran, cotton bran, triturated Guandu hay and disintegrated corn with its straw and corncob (according to requirements for due preservation and production). The mean values found were: sodium, $45{\pm}10.2mg%$ (24.5-65.6 mg%); potassium, $206{\pm}44.7mg%$ (116.6-295 mg%); calcium, $111{\pm}22mg%$ (66.5-155.4 mg %); ashes, $0.82{\pm}0.06mg%$ (0.68-0.95%); chloride, $235{\pm}39mg%$ (157-313 mg%). Results were related to: a) breed, the Brown and the Saanen revealed superior values for calcium and ashes, b) climate, which in the rain season led to higher contents of sodium, potassium, and calcium when compared to the local dry season, c) time of lactation which increasingly affected the contents of calcium, sodium and ashes, and d) the time between milking, when higher concentration of chloride and ashes were mostly concentrated in the evening and calcium in the morning. These results have important implications in the handling and management of the goat herd.

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.36-40
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• 2007

Proteins and lipids not only provide a source of energy to the cell, but also play vital roles in modifying the physical properties and function of the biological membranes. In the present study, we investigated the biochemical constituents, viz. proteins and lipids, in growing oocytes of goat antral follicles during summer and winter seasons. Goat genitalia in phosphate buffered saline (pH 7.4) were brought to the laboratory within one hour of slaughter under aseptic conditions at $37^{\circ}C$. Oocytes were aspirated from normal small (<3 mm in diameter) and large (>3 mm) follicles and pooled for biochemical estimations. A significant increase in the amount of protein and lipid was observed with the growth of the oocyte. The amount of protein varied non-significantly with the season, while the amount of lipid varied significantly. The amounts of phospholipid, cholesterol, free fatty acid, and triglyceride increased with the growth of the oocyte, but no significant effect of season in these constituents was observed. Lysolecithin, sphingomyelin, and sterols were the polar lipids identified in both oocytes prepared from small follicles (small oocytes) as well as large follicles (large oocytes). In addition, the small oocytes also contained phosphatidyl serine, while large oocytes contained phosphatidyl glycerol phosphate and phosphatidyl inositol. Among non-polar lipids, triglycerides and long chain alcohols appear only in small oocytes and not in large oocytes. Monoglycerides, 1,2-diglycerides, 1,3-diglycerides and o-dialkyl glycerol ethers, fatty acids, fatty acid methyl esters, and wax esters were identified in both small and large oocytes. Information on biochemical composition of growing oocytes is relevant to oocyte and embryo competence, culture and cryopreservation.

• Asian-Australasian Journal of Animal Sciences
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• 제22권9호
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• pp.1234-1240
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• 2009

Gene flow, genetic structure and differentiation of Kutchi, Mehsana and Sirohi breeds of goat from North-Western India were evaluated based on 25 microsatellite markers so as to support breed conservation and improvement decisions. The microsatellite genotyping was carried out using an automated DNA sequencer. The gene diversity across the studied loci for the Kutchi breed varied from 0.57 (ILST 065) to 0.93 (OarFCB 304, OMHC 1, ILSTS 058) with an overall mean of 0.79${\pm}$0.02. The corresponding values for Mehsana and Sirohi breeds were 0.16 (ILST 008) to 0.93 (OMHC 1, ILSTS 058) with an average of 0.76${\pm}$0.04, and 0.50 (ILSTS 029) to 0.94 (ILSTS 058) with an average of 0.78${\pm}$0.02, respectively. The Mehsana breed had lowest gene diversity among the 3 breeds studied. All the populations showed an overall significant heterozygote deficit ($F_{is}$). The Fis values were 0.26, 0.14 and 0.36 for Kutchi, Mehsana and Sirohi goat breeds, respectively. Kutchi and Mehsana were more differentiated (16%) followed by Mehsana and Sirohi (13%).The measures of standard genetic distance between pairs of breeds indicated that the lowest genetic distance was between Kutchi and Sirohi breeds (0.73) and the largest genetic distance was between Mehsana and Kutchi (1.0) followed by Sirohi and Mehsana (0.75) breeds. Mehsana and Kutchi are distinct breeds and this was revealed by the estimated genetic distance between them. All measures of genetic variation revealed substantial genetic variation in each of the populations studied, thereby showing good scope for their further improvement.

• 대한수의학회지
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• 제48권1호
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• pp.83-88
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• 2008

In total, 1,142 serum samples were collected from 223 goat flocks rising in five different regions of Korea. These samples were screened for the presence of border disease virus (BDV) antibodies using an enzyme linked immunosorbent assay. Of the 1,142 samples, we found 47 bovine viral diarrhea virus (BVDV) positive cases (4.1%). These positive serum samples were also examined further by using the virus neutralization test against BDV. In addition, samples were tested for both BVDV and classical swine fever virus (CSFV). All of the samples that were seropositive for BDV also demonstrated positive antibody titers against BVDV and CSFV. Due to their common antigenicity, we also determined further the prevalence and carried out virus neutralization test against three pestiviruses: 314 of the goat samples were screened using reverse transcription polymerase chain reaction with primer pairs specific to common pestivirus genome regions. Overall, 1.6% (5/314) of the samples tested was positive for pestivirus. Based on the nucleotide sequence data and the phylogenetic analysis, three isolates were characterized as BVDV type 1 and two isolates as BVDV type 2. However, none of the isolates could be classified as BDV. These results indicate that BVDV-1 and BVDV-2 are the pestivirus strains circulating among Korean goat populations.

• 대한수의학회지
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• 제53권4호
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• pp.273-276
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• 2013

Two Korean black goat (approx. 2 and 3 years old) showing diarrhea and chronic weight loss were submitted to Animal and Plant Quarantine Agency. At necropsy, there were thickening of small intestine and enlargement of mesenteric lymph nodes. Microscopically, they had granulomatous enteritis in the small and large intestine and granulomatous lymphadenitis. By polymerase chain reaction (PCR) and acid fast stain, strong positive reaction and acid-fast rod bacteria were detected. According to the result of histopathology and PCR, we confirmed this case as Johne's disease. As far as we know, this is the first report of Johne's disease in Korean black goat.

• 한국초지조사료학회지
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• 제15권4호
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• pp.297-302
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• 1995

The objective of this experiment was to compare the dry matter intake, nutrients digestibility, nitrogen and energy utilization of hehage among the orchardgrass(OG) pure, orchardgrass(OG)-red clover(RC) mixture and complex rnixtures(C. mix) by Korean native goat. The results obtained were surnmerized as follows: 1. Crude protein(CP) content was higher in OG-RC and C. Mix diets than in OG pure diets(P<0.05). NDF content was higher in OG pure diets than other diets(P<0.05). But ADF, Lignin contents and gross energy did not differ among all diets. 2. Dry matter intake by Korean native goat tend to be high in OG-RC diets, and low in OG pure diets. But there was no significant difference statistically. Dry matter and cellular constituents digestibilities were higher for OG-RC and C. Mix diets than OG pure diet, but CP and NDF digestibilities were no significant difference among diets. 3. The amount of nitrogen consumed amount by Korean native goat in the OG-RC diet was higher than other diets. But, due to largely relative urinary and fecal nitrogen loss, Apparrently digested nitrogen and retained nitrogen percents were not significant difference among diets. 4. The amount of energy consumed by Korean native goat in OG-RC diet was higher than other diets. But, due to largely relative urinary and fecal energy loss, digestable energy percent was lower than C. Mix diets. But.metabolic energy percent was no significant difference among diets. Therefore, there was no significant differences among sward types for the utilization of nitrogen and energy by Korean native goats.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.340-345
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• 2002

In this experiment, oocytes were collected from goat ovaries available in slaughterhouse by follicle puncture method. Morphologically culturable type of oocytes which having compact, multilayered cumulus granulosa cell complex and evenly granulated cytoplasm, was separated under a stereozoom microscope. Oocytes were washed thoroughly in maturation medium containing TCM-199, $1{\mu}g/ml$ estradiol-$17{\beta}$, 0.5 ${\mu}g/ml$ FSH, $100{\mu}g/ml$ LH, 3 mg/ml BSA and 10% estrus goat serum. Washed oocytes were cultured into maturation medium on granulosa cell monolayer. Culture plate was then kept into $CO_2$ incubator at $38{\pm}1^{\circ}C$, maximum humidity and 5% $CO_2$ for 18 h. After maturation the oocytes were washed thoroughly with maturation medium containing polyvinyl alcohol (PVA) without serum and BSA and further cultured for 12 h for secretory proteins of oocytes. PVA medium was collected, pooled and concentrated by 5000 cut off centrisart. Secretory proteins were separated on 12.5% SDS-PAGE. A total number of 3.41 oocytes per ovary were obtained and 2.17 culturable oocytes per ovary were cultured into maturation medium. After 18 h of maturation, 4,567 oocytes (1.82 oocytes per ovary) were further cultured into serum and BSA free PVA medium for its secretory proteins. Four secretory proteins of oocytes with approximately molecular weight of 45, 55, 65 and 95 kDa were obtained on SDS-PAGE in silver staining and three proteins with approximately molecular weight of 45, 55 and 65 kDa in Coomassie brilliant blue staining. In conclusion, four secretory proteins with approximately molecular weight of 45, 55, 65 and 95 kDa was obtained from in vitro cultured oocytes of goats.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.555-559
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• 1993

Studies at freezing point depression (FPD) for goat milk are described in this paper. The 432 samples of raw goat milk collected in a period of two years and analysed by the use of an electronic cryoscope gave a mean value of FPD: $-0.565{\pm}0.01^{\circ}C$. Statistical significant differences were found between the morning and evening milk. The evening milk showed better quality considering the lactose and others dissolved solutes. Among the Brown, Saanen and Anglo-nubian goats, the greatest increase in FPD, occurred in the Anglo-nubian breed. The statistical analysis revealed a significant breed difference (p<0.05), when climate variation was observed. The superior and inferior values found to the FPD of the caprine milk (-0.545 to $-0.585^{\circ}C$), is important in establishing regional rules, to improve the quality control criteria and legal interpretation of standards found in the pertinent legislation. The results are also important to improve herd management so that one can increase the animal production which will affect the technological composition, and the nutritional characteristics of the resultant products.

• Asian-Australasian Journal of Animal Sciences
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• 제22권3호
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• pp.428-432
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• 2009

40 goat kid ribcages were held for 7 days in storage conditions ($4^{\circ}C$) and used to determine the effects of three different packaging methods (atmospheric air, vacuum and modified atmosphere package (MAP) 10:70:20 mixture of $N_2:O_2:CO_2$) on meat quality of the chops. L* was affected by the packaging method being lighter than MAP chops. The coordinate a* significantly increased during storage time. For MAP-packed chops and those kept in atmospheric air, b* increased markedly during storage time whereas it remained unaffected throughout storage when in vacuum packages. Final pH values ranged from 5.6 to 5.8 and no effects were found for either storage time or packaging method. WHC means were lowest for the three packaging methods on day 7 of storage and highest on day 1. Storage time increased water loss in vacuum treatments. Trained panel colour acceptability was lower at 3, 5 and 7 days than on day 1 of storage for atmospheric air treatment and vacuum packaging, while for the MAP treatment average values on days 5 and 7 were lower than those observed on days 1 or 3. Trained panel odour was lower for atmospheric air and vacuum packages at 3, 5, and 7 days storage than at 1 day, while no differences were found in trained panel odour acceptability for MAP packages. With reference to consumers, the MAP proposed in the present study is the chosen method for storing goat meat, rather than vacuum or atmospheric air packaging.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.606-612
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• 2012

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.