• Title/Summary/Keyword: Glycyrrhiza glabra

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Development of natural sunscreen using plant extracts (식물 추출물을 이용한 천연 자외선 차단제 개발)

  • Moon, Ji-Sun
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.5
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    • pp.1138-1150
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    • 2020
  • In this study, an experiment was conducted to develop a sunscreen with antioxidant effects by simultaneously investigating the antioxidant and UV protection capabilities of various plant extracts. First, to investigate the UV-blocking ability of 33 kinds of plant extracts, the absorbance spectrum between the UV wavelength of 280 to 400 nm was investigated. Arrowroot, graviola, wheat sprout, sangbaek skin, thorn meal, lacquer, etc. 11 species were selected. The total polyphenol content, total flavonoid content, and DPPH radical scavenging activity of the selected plant extracts are measured to examine the degree of antioxidant activity, and from this, it is a plant extract that has excellent UV protection and antioxidant activity at the same time. The species was selected. A gel-shaped cream is prepared by mixing the selected gold, hops, and licorice extracts in a ratio of 1:1:1, and the UV protection effect of this cream is measured when the cultured cells are irradiated with UV rays. Determined by the method. As a result of the study, it was confirmed that the selected mixture of plant extracts complemented each other in terms of ultraviolet absorption ability and increased cell damage protection effect. Through these results, it was confirmed that it was possible to develop a sunscreen with an antioxidant effect if the antioxidant and sunscreen capabilities of various plant extracts were determined at the same time.

Biological Activities of Licorice F1 Lines and Content Analysis of Phytochemical Constituents

  • Park, Chun-Geon;Lee, Ah Young;Lee, Jeong Hoon;Lee, Jeong Min;Park, Jun Yeon;Lee, Sang-Hoon;Choi, Ae Jin;Park, Chung Berm;Cho, Eun Ju;Lee, Sanghyun
    • Natural Product Sciences
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    • v.20 no.3
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    • pp.137-145
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    • 2014
  • The biological activities of licorice F1 (Glycyrrhiza glabra ${\times}$ G. uralensis) lines (G) were investigated, revealing strong radical scavenging activity targeting 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (${\cdot}OH$) radicals. At a concentration of $100{\mu}g/mL$, most of the licorice F1 lines scavenged DPPH and ${\cdot}OH$ by more than 80%. Gs-1, -2, and -6 can be considered good scavengers of DPPH radical and G-7 have higher antioxidant activity against ${\cdot}OH$ radical. In addition, licorice F1 lines exerted effective anti-microbial activities against Escherichia coli (Gs-12, -17, and -18) and Staphylococcus aureus (Gs-3, -4, -5, -21, and -26). Moreover, Gs-2, - 20, -31, and -32 effectively inhibited the growth of Helicobacter pylori. Among licorice F1 lines, Gs-25 exhibited high anti-inflammatory effects on nitric oxide produced by lipopolysaccharide- and interferon-${\gamma}$-activated RAW 264.7 cells. Furthermore, Gs-1, -12, and -20 inhibited the growth of AGS human gastric adenocarcinoma cells by more than 60% at a concentration of $100{\mu}g/mL$ and Gs-5, -11, -19, and -32 showed inhibitory effects against rat lens aldose reductase ($IC_{50}$ values, 1.69, 6.07, 6.12, and $4.54{\mu}g/mL$, respectively). The total content of glycyrrhizin (1), glycyrrhetinic acid (2), glabridin (3), and isoliquiritigenin (4) in licorice F1 lines was high in Gs-11, -15, and -30. The present study therefore indicated that Gs-2, -26, -31, and -32 of licorice F1 possessing strong anti-oxidative, anti-microbial, anti-inflammatory, anti-cancer, and aldose reductase inhibitory effects may be used as a possible source material for natural health supplements in the future.

Evaluation of the Estrogenic and Antioxidant Activity of Some Edible and Medicinal Plants (식용 및 약용자원의 에스트로젠 활성과 항산화능 평가)

  • Choi, Sun-Young;Lim, Sun-Hye;Kim, Ji-Sun;Ha, Tae-Youl;Kim, Sung-Ran;Kang, Kyung-Sun;Hwang, In-Kyeong
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.549-556
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    • 2005
  • Estrogenic and antioxidant activities of ethanol extracts of 45 edible and medicinal plants were evaluated by ${\beta}-galactosidase$ assay, and DPPH radical scavenging assay, and TBARS inhibition rate, respectively. Total polyphenol contents were in the range of 8.6 (Panax notoginseng Buck F.H. Chen.)-594.7 (Amomum globosum Loureiro) mg/g. Direct correlation between the DPPH radical scavenging activity and polyphenol content $(r^2=0.61)$ was established through simple regression analysis, whereas no correlation was observed between TBARS inhibition rate or ${\beta}-galactosidase$ activity and polyphenol content. Among medicinal plants screened, Glycyrrhiza glabra L. and Rheum undulatum L. showed strong antioxidant and estrogenic activities. Results of this study could be used as fundamental data for selecting potential phytoestrogen candidates.

Anti-cancer effect of glabridin by reduction of extracellular vesicles secretion in MDA-MB-231 human breast cancer cells (유방암세포에서 세포외 소포체 분비 감소를 통한 glabridin의 항암효과)

  • Choi, Sang-Hun;Hwang, Jin-Hyeon;Baek, Moon-Chang;Cho, Young-Eun
    • Journal of Nutrition and Health
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    • v.55 no.2
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    • pp.240-249
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    • 2022
  • Purpose: Glabridin (GD) is a bio-available isoflavane isolated from the root extract of licorice (Glycyrrhiza glabra L.). It exhibits a variety of pharmacological activities such as anti-inflammatory and anti-oxidant activities. However, extracellular vesicles (EVs) secretion and the anti-cancer mechanism of action remains largely unknown. The present study investigates the anticancer effects of GD by determining the inhibition of EVs secretion in the human breast cancer cell line, MDA-MB-231. Methods: Cell viability, reactive oxygen species (ROS) production, migration, invasion rate, and vascular endothelial growth factor (VEGF) concentration were assessed in MDA-MB-231 cells treated with increasing concentrations of GD (0.1, 1, 5, 10, 20 µM). Subsequently, EV secretion and exosomal DEL-1 protein expression were evaluated to determine the anticancer effects of GD. Results: The results showed that GD significantly inhibited the cell proliferation of MDA-MB-231 cells in a dose- or time-dependent manner. Also, ROS production and apoptosis marker protein cleaved caspase-3 were significantly increased in GD-treated MDA-MB-231, compared to control. Furthermore, GD exposure resulted in significantly decreased not only migration and invasion rates but also the VEGF concentration, thereby contributing to a reduction in angiogenesis. Interestingly, the concentration and number of EVs as well as EV marker proteins, such as CD63 and TSG101, were decreased in GD-treated MDA-MB-231 cells. Markedly, extracellular matrix protein DEL-1 as angiogenesis factor was decreased in EVs from GD-treated MDA-MB-231 cells. Conclusion: This study identifies that the anti-cancer molecular mechanism of GD is exerted via inhibition of angiogenesis and EVs secretion, indicating the potential of GD as a chemotherapeutic agent for breast cancer.

Studies on the Development of Natural Preservatives from Natural Products (전통식품 및 천연물에서 천연보존료 개발에 관한 연구)

  • Kim, Hee-Yun;Lee, Young-Ja;Hong, Ki-Hyoung;Kwon, Yong-Kwan;Lee, Ju-Yeun;Kim, So-Hee;Ha, Sang-Chunl;Cho, Hong-Yon;Chang, Ih-Seop;Lee, Chul-Won;Kim, Kil-Saeng
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1667-1678
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    • 1999
  • Certain parts of 190 kinds of medicinal herbs and 171 kinds of original materials of food were extracted by methanol. The extracts were tested their microbial inhibition activities against several food spoilage microorganisms, Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, Saccharomyces cerevisiae, Candida albicans, Penicillium citrinum, Aspergillus flavus and Aspergillus niger. The methanol extracts of Cornus officinalis, Evodia officinalis, Glycyrrhiza glabra, Salvia miltiorrhiza. Schizandrae fructus, Coptidis rhizoma, aroma hop and bitter hop were shown inhibitory effect on certain species of gram(+) bacteria. Aroma hop and bitter hop were shown inhibitory effect on certain species of gram(-) bacteria. The methanol extract of Salvia miltiorrhiza exhibited a strong antibacterial activities. It was purified by solvent fractionation, silicagel column chromatography, prep. TLC, prep. HPLC. The purified active substance was identified as cryptotanshinone by EIMS, $1^H-NMR,\;{13}^C-NMR$ and DEPT. Cryptotanshinone showed a strong antibacterial activity against gram positive bacteria $(MIC\;:\;3.91{\sim}62.50\;{\mu}g/mL)$. Especially, this compound was the most strong activity against Bacillus subtilis $(MIC\;:\;3.91\;{\mu}g/mL)$.

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