• Title/Summary/Keyword: Glutamic Acid

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Studies on the Synthesis of L(+)-Glutamic Acid Derivatives(II) (L(+)-Glutamic Acid 유도체의 합성(II))

  • 조윤상
    • YAKHAK HOEJI
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    • v.23 no.2
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    • pp.125-128
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    • 1979
  • In the formation of L(+) -glutamine from L(+) -glutamic acid-5-hydrazide, large amount of Raney-Ni was effective under normal pressure but hydrogenation or amonolysis of ester under pressure was useless. Preparation of glutamine with .alpha.-ketoglutaric acid (by way of 1, 4, 5, 6-tetrahydro-6-oxo-3-pyridazine carboxylic acid) is intersting but not so efficient in yield.

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Studies on the Production of L-Glutamic Acid by Brevibacterium ammoniagenes (Brevibacterium ammoniagenes에 의한 글루탐산 제조에 관한 연구)

  • Yoo, Young-Jin;Kim, Taik-Yung
    • Korean Journal of Food Science and Technology
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    • v.9 no.1
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    • pp.1-9
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    • 1977
  • A bacterium strain (K-173-10) which was isolated from waste soil of Korea brewing factory, could be grown on acetate as the sole carbon source and accumulated a considerable amount of L-glutamic acid in the medium. This strain was identified as the new species Brevibacterium ammoniagenes. This study was concerned not only with the culture condition for the production of L-glutamic acid and the cell growth, but also with the effects on concentration of various kind of organic substances, growth factors and penicillin. The results obtained were summarized as follow; 1. It was found that the concentrations of acetate and ammonium ions affected the growth of the bacterium as well as its L-glutamate accumulation. The optimum conditions of the composition of grown media for the growth of the bacterium and its glutamic acid production was found to be 40 g/l of total acetate, $100\;{\mu}g/l$ thiamine, $0.5\;{\mu}g/l$ biotin and $1{\sim}2g/l$ corn steep liquor as the growth factors. 2. Organic acid such as succinic acid, malic acid and ${\alpha}-ketoglutaric$ acid inhibited the cell growth as well as its L-glutamic acid production. 3. The penicillin (20 units/ml) stimulated the production of glutamic acid at appropriate incubation period. 4. It was found that this strain could grow in the presence of urea and ammonium acetate but not in other nitrogen sources.

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Glutamic Acid-Grafted Metal-Organic Framework: Preparation, Characterization, and Heavy Metal Ion Removal Studies

  • Phani Brahma Somayajulu Rallapalli;Jeong Hyub Ha
    • Applied Chemistry for Engineering
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    • v.34 no.5
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    • pp.556-565
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    • 2023
  • Fast industrial and agricultural expansion result in the production of heavy metal ions (HMIs). These are exceedingly hazardous to both humans and the environment, and the necessity to eliminate them from aqueous systems prompts the development of novel materials. In the present study, a UIO-66 (COOH)2 metal-organic framework (MOF) containing free carboxylic acid groups was post-synthetically modified with L-glutamic acid via the solid-solid reaction route. Pristine and glutamic acid-treated MOF materials were characterized in detail using several physicochemical techniques. Single-ion batch adsorption studies of Pb(II) and Hg(II) ions were carried out using pristine as well as amino acid-modified MOFs. We further examined parameters that influence removal efficiency, such as the initial concentration and contact time. The bare MOF had a higher ion adsorption capacity for Pb(II) (261.87 mg/g) than for Hg(II) ions (10.54 mg/g) at an initial concentration of 150 ppm. In contrast, an increased Hg(II) ion adsorption capacity was observed for the glutamic acid-modified MOF (80.6 mg/g) as compared to the bare MOF. The Hg(II) ion adsorption capacity increased by almost 87% after modification with glutamic acid. Fitting results of isotherm and kinetic data models indicated that the adsorption of Pb(II) on both pristine and glutamic acid-modified MOFs was due to surface complexation of Pb(II) ions with available -COOH groups (pyromellitic acid). Adsorption of Hg(II) on the glutamic acid-modified MOF was attributed to chelation, in which glutamic acid grafted onto the surface of the MOF formed chelates with Hg(II) ions.

Effects of Nicotinic Acid Deficiency on the Levels of Various Metabolites in the Serum of Quail (Nicotinic acid 결핍이 메추리 혈청의 여러 대사물질 수준에 미치는 효과)

  • 이재혁;박인국
    • The Korean Journal of Zoology
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    • v.34 no.2
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    • pp.203-208
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    • 1991
  • Body weight gain in the niadn deficient group of quail was markedly lowered as compared to that of the control group, but heart, kidney and liver weight were slighdy reduced reladve to the body weight. Nicotinic acid deficiency resulted in the significant increase of serum glucose level but the serum cholesterol, albumin and total protein levels were not affected to any extent. Glutamic oxaloacetate iransaminase and glutamic pyruvate transaminase activities were significandy enhanced but alkaline phosphatase and lactic dehydrogenase activities were not influenced. Tryptophan and tyrosine levels were remarkably reduced and a similar observation was also made with aspartic acid, glutamic acid and alanine plus serine. However, the levels of basic amino acids such as arginine, histidine and lysine plus branched chain amino acids such as isoleucine, leucine and valine were not affected.

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Identification of Growth Stimulatory Compound in the Mixed Culture of Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1 in Milk (Lactobacillus helveticus YM-1 과 Streptococcus thermophilus CH-1의 혼합배양액 중에 함유된 생육촉진물질의 확인)

  • Yoon, Sung-Sik;Yu, Ju-Hyun
    • Korean Journal of Food Science and Technology
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    • v.18 no.6
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    • pp.492-496
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    • 1986
  • A compound stimulatory to the growth of S. thermophilus CH-1 was isolated from the cell-free filtrate of L. helveticus YM-1 in milk medium. The stimulant was identified as a peptide with a molecular weight of approximately 5000 and exhibited positive ninhydrin reaction. Some kinds of amino acids confirmed as aspartic acid, alanine, valine, glutamic acid, lysine, proline, leucine were rich in the stimulatory peptide hydrolysate. Among them, glutamic acid was most abundant. Judging from bioautographic results, glutamic acid and phenylalanine were expected to exert an important role for the stimulation.

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Effects of Germination in Brown Rice by Addition Chitosan/Glutamic acid (Chitosan/Glutamic acid 처리가 현미의 발아에 미치는 영향)

  • Jung Gyu-Ho;Park Nan-Young;Jang Sang-Moon;Lee Joo-Baek;Jeong Yong-Jin
    • Food Science and Preservation
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    • v.11 no.4
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    • pp.538-543
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    • 2004
  • To improve the levels of ${\gamma}$-aminobutyric acid (GABA) in germinated brown rice, chitosan and glutamic acid were treated during the brown rice germination. The GABA contents in germinated brown rices were 425.7 nmole/g and 637.0 nmole/g at germination temperature of $25^{\circ}C$ and germination time of 72 hrs. Response surface methodology(RSM) was used to monitor characteristics of germination from brown rices. As glutamic acid and chitosan concentration were increased, the GABA content was also increased. The ranges of optimum conditions were $105{\sim}160\;ppm$ in chitosan concentration and $200{\sim}290\;ppm$ in glutamic acid concentration. Predicted values at the optimized conditions were acceptable in comparison with experimental values.

Enzymatic Degradation of Poly(${\gamma}$-glutamic acid) Hydrogel Prepared by ${\gamma}$-Ray Irradiation

  • Hara, Toshio;Choi, Seong-Hyun;Choi, Woo-Young
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.342-345
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    • 2001
  • A bacterial strain PH-4, which produces an enzyme catalyzing the degradation of crosslinked poly(${\gamma}$-glutamic acid) hydrogels, was isolated and identified as a Flavobacterium sp. The enzyme was obtained by the sonication of the bacterial cells preincubated in a Bouillon medium with shaking, without adding of poly(${\gamma}$-glutamic acid) as an inducer. The products of the hydrogel degraded by the crude enzyme agreed closely with the depolymerized materials in SDS-polyacrylamide gel electrophoresis using methylene blue staining, and with a glutamic acid monomer on thin-layer chromatography, thereby suggesting that strain PH-4 produced a kind of exohydrolase.

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Effect of plasmid curing on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W (글루탐산을 유일한 탄소 원과 질소 원으로 이용하는 Acinetobacter sp. B-W의 글루탐산으로부터의 시드로포어 생산에 미치는 플라스미드 제거 효과)

  • Kim, Kyoung-Ja;Lee, Jae-Rim;Yang, Yong-Joon
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.266-271
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    • 2018
  • Effect of plasmid curing of Acinetobacter sp. B-W on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources was investigated. Plasmid cured mutant of strain B-W lost the ability to produce siderophore from glutamic acid at $28^{\circ}C$. Transformant E. coli $DH5{\alpha}$ harboring 20 kb plasmid, that was isolated from wild type of strain B-W produced siderophore from glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$, but, not at $36^{\circ}C$. Production of siderophore from glutamic acid by transformant E. coli $DH5{\alpha}$ was completely inhibited by $10{\mu}M\;FeCl_3$. In previous report, catechol nature of siderophore produced from glutamic acid by strain B-W was detected by Arnow test. The siderophore produced from glutamic acid by transformant E. coli $DH5{\alpha}$ was also catechol type. Rf value of siderophore produced from transformant E. coli $DH5{\alpha}$ grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32 in butanol-acetic acid-water (12:3:5) as developing solvent. Rf value of the siderophore was the same with that of wild type of strain B-W. Thus a single plasmid of 20 kb seemed to be involved in the production of siderophore from glutamic acid.

Effect of Glutamic Acid on Polysaccharide Production from Agaricus blazei in Liquid Culture

  • Hwang, Jeong-Min;Im, Yeong-Su;Gwon, Myeong-Sang;Choe, Jeong-U;Han, Jin-Su;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.231-234
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    • 2000
  • The polysaccharide has been known to have an antitumor activity, which were extracted from the fruiting bodies, mycelia, and culture broths of Agaricus blazei. For the cell growth and the polysaccharide production, the optimal medium contained 8% glucose, 2% yeast extract, 0.1% $MgSO_4{\cdot}7H_2O$, 0.2% $KH_2PO_4$, 0.2% $CaCl_2{\cdot}2H_2O$ and 0.2% $MnSO_4{\cdot}5H_2O$. When 0.2% of glutamic acid was added at 4day, the cell concentration was 13.5 g/L and the polysaccharide production was 9.9 g/L, respectively.

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