• 한국잠사곤충학회지
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• 제41권3호
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• pp.129-134
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• 1999

The purpose of this study is to investigate the effects of the ice-cream containing mulberry leaves on glucose levels in blood and on sensory characteristics. Mulberry leaves were treated with sodium hydrogencarbonate (NaHCO3) to remove the fresh green note out of dried mulberry powder with ball mill. The recovery rate of dried mulberry leaf powder was 28.9% from fresh mulberry leaves. The points of ice-cream with (treatment 2 : milk-fatty 23%) and without (treatment 7 : coconut butter 9.5%) milk-fatty in the sensory characteristics were 8.31 and 8.47, respectively, of 9.00 as a full point. The range of age of volunteers taken part in the glucose level test were 21∼25 years old. The glucose levels in blood were measured before and after taking up ice-cream. In case of ice-cream with vanilla, the glucose levels increased 27.8%, while those in the up take of ice-cream with mulberry powder decreased 1.6%. In conclusion, the present study has demonstrated that ice-cream containing mulberry leaf powder has an effect to decrease blood glucose levels after the application.

• 동아시아식생활학회지
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• 제12권3호
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• pp.235-240
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• 2002

In this study, rice candy was produced with mulberry leaf and its effect on the lowering blood glucose level was assessed. The results were as follows: The recovery rate of mulberry leaf powder was 32.7%. The contents of crude protein, lipid, ash and crude fiber of mulberry leaf were 19.54%, 4.82%, 12.80%, and 11.27%, respectively. The sensory evaluation revealed that the rice candy added with 1% mulberry leaf powder showed slightly hi인or palatability score than that of 2%, but we decided that 2% addition of mulberry leaf powder would be preferred over 1% if considering both the physiological function and the sensory acceptability. The blood glucose level after ingesting the rice candy with mulberry leaf lowered by 31%, more than that of control.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권3호
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• pp.210-212
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• 2003

Recombinant $\beta$-glucosidase from Thermus caldophilus GK24 was easily purified partially by a heat treatment procedure, resulting in 8-fold and recovery yield of 80% from crude enzyme. When the $\beta$-glucosidase was incubated with a 80% glucose solution (w/w), gentiobiose ($\beta$1,6-glucobiose) was the major product in the reaction mixture. The optimal conditions for producing gentiobiose (11% yields of total sugar) were pH 8-9 and 7$0^{\circ}C$ for 72 h.

• 보건교육건강증진학회지
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• 제14권1호
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• pp.173-182
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• 1997

This study presents the results observed in the change in blood components of ten female students of “K” university's physical Education Department during submaximal exercise, relaxation and recovery periods. 1. After ecercise, the WBC value is higher than in relaxation time. Also within thirty minutes of the relaxation period it does not return to the normal range. 2. After exercise, the RBC value is higher than during relaxation time. Also in the recovery period, within 30 minutes it returns to the normal range. 3. After exercise. the RCT value is higher than during relaxation time. Also in the 30 minutes recovery period it returns to the normal range of relaxation. 4. After exercise, the Hb value is higher than during relaxation time. It rises slowly after exercise and returns to the relaxation range in the 30 minutes recovery period. 5. After exercise and in 10 minutes of the recovery period, the value of Glucose is lower than during relaxation time. It returns to the relaxation range in 30 minutes of the recovery period. 6. After exercise the value of protein is higher than during relaxation time. It returns to the relaxation range within ten to thirty minutes of the recovery period.

• Journal of Chest Surgery
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• 제24권11호
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• pp.1058-1067
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• 1991

High potassium cardioplegia is a widely accepted procedure to enhance myocardial protection from ischemic injuries associated with open heart surgery. Maintaining optimum osmolarity of the cardioplegic solution is one of the required conditions for an ideal cardioplegic solution Albumin is an frequently added component for maintaining optimum osmolarity of clinically used cardioplegic solutions. But the source of albumin is human blood so that the supply is limited and the cost of manufacturing is relatively high. Recently there are moves to minimized the use of blood product for fear of blood-associated infections or immunological disorders. In this experiment, we substituted mannitol or glucose for albumin added to the cardioplegic solution which has been used at the Wonju Medical College, To determine whether addition of mannitol or glucose instead of albumin in the cardioplegic solution can produce satisfactory myocardial protection during ischemia, three different groups of isolated rat heart perfused by modified Langendorff technique were studied. Wonju Cardioplegic Solution was selected as a standard high potassium[18mEq/L of K+] cardioplegic solution. Three kinds of cardioplegic solution were made by modifying the composition maintaining the same osmolarity[339$\pm$1mOsm/Kg] Isolated rat heart were perfused initially with retrograde nonworking mode and then changed to working mode. After measuring the heart rate, systolic aortic pressure, aortic flow, coronary flow, ischemic arrest by aorta cross clamp and cardioplegia was made maintaining the temperature of water jacket at 10oC. The heart was rewarmed and reperfused after 60min of ischemic arrest with intermittent cardioplegia at the 30min interval. The time to return of heart beat and the time required to get. Regular heart beat were observed after reperfusion. The recovery rate of the functional variables-heart rate, systolic aortic pressure, aortic flow, coronary flow and cardiac output were calculated and compared among the three groups of different cardioplegia-albumin, mannitol, and glucose. The wet weight and dry weight was measured and the water content of the heart as figured out for comparison. The time to return of heart beat was fastest in the albumin group, The functional recovery rates were best in the albumin group also. In the above conditions, albumin was the best additive to the cardioplegic solution compared to the mannitol or glucose.

• 운동영양학회지
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• 제16권2호
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• pp.65-71
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• 2012

Oxygen is the final acceptor of electron transport from fat and carbohydrate oxidation, which is the rate-limiting factor for cellular ATP production. Under altitude hypoxia condition, energy reliance on anaerobic glycolysis increases to compensate for the shortfall caused by reduced fatty acid oxidation [1]. Therefore, training at altitude is expected to strongly influence the human metabolic system, and has the potential to be designed as a non-pharmacological or recreational intervention regimen for correcting diabetes or related metabolic problems. However, most people cannot accommodate high altitude exposure above 4500 M due to acute mountain sickness (AMS) and insulin resistance corresponding to a increased levels of the stress hormones cortisol and catecholamine [2]. Thus, less stringent conditions were evaluated to determine whether glucose tolerance and insulin sensitivity could be improved by moderate altitude exposure (below 4000 M). In 2003, we and another group in Austria reported that short-term moderate altitude exposure plus endurance-related physical activity significantly improves glucose tolerance (not fasting glucose) in humans [3,4], which is associated with the improvement in the whole-body insulin sensitivity [5]. With daily hiking at an altitude of approximately 4000 M, glucose tolerance can still be improved but fasting glucose was slightly elevated. Individuals vary widely in their response to altitude challenge. In particular, the improvement in glucose tolerance and insulin sensitivity by prolonged altitude hiking activity is not apparent in those individuals with low baseline DHEA-S concentration [6]. In addition, hematopoietic adaptation against altitude hypoxia can also be impaired in individuals with low DHEA-S. In short-lived mammals like rodents, the DHEA-S level is barely detectable since their adrenal cortex does not appear to produce this steroid [7]. In this model, exercise training recovery under prolonged hypoxia exposure (14-15% oxygen, 8 h per day for 6 weeks) can still improve insulin sensitivity, secondary to an effective suppression of adiposity [8]. Genetically obese rats exhibit hyperinsulinemia (sign of insulin resistance) with up-regulated baseline levels of AMP-activated protein kinase and AS160 phosphorylation in skeletal muscle compared to lean rats. After prolonged hypoxia training, this abnormality can be reversed concomitant with an approximately 50% increase in GLUT4 protein expression. Additionally, prolonged moderate hypoxia training results in decreased diffusion distance of muscle fiber (reduced cross-sectional area) without affecting muscle weight. In humans, moderate hypoxia increases postprandial blood distribution towards skeletal muscle during a training recovery. This physiological response plays a role in the redistribution of fuel storage among important energy storage sites and may explain its potent effect on changing body composition. Conclusion: Prolonged moderate altitude hypoxia (rangingfrom 1700 to 2400 M), but not acute high attitude hypoxia (above 4000 M), can effectively improve insulin sensitivity and glucose tolerance for humans and antagonizes the obese phenotype in animals with a genetic defect. In humans, the magnitude of the improvementvaries widely and correlates with baseline plasma DHEA-S levels. Compared to training at sea-level, training at altitude effectively decreases fat mass in parallel with increased muscle mass. This change may be associated with increased perfusion of insulin and fuel towards skeletal muscle that favors muscle competing postprandial fuel in circulation against adipose tissues.

• 한국양식학회지
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• 제22권1호
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• pp.63-67
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• 2009

In order to establish optimum anesthesia concentration, we tested the efficacy of clove oil at five different concentrations in large sized (mean SL $17.1{\pm}2.21\;cm$) and small sized (mean SL $0.6{\pm}0.06\;cm$) dark-banded rockfish Sebastes inermis. Optimal anesthesia concentration for dark-banded rockfish was $150\;mgL^{-1}$ in both large and small sized fish. In general, fish exposed to higher anesthetic doses were rapidly induced but took longer to recover (P<0.05). Recovery time of small sized fish was longer than large sized fish in lower concentrations, while recovery time of large sized fish was longer than small sized fish in higher concentration (P<0.05). Using the established optimum aesthetic concentration, we evaluated the physiological response of dark-banded rockfish to clove oil by measuring plasma cortisol and glucose levels. Following administration of $150\;mgL^{-1}$ clove oil at $20^{\circ}C$ (optimum breeding temperature), plasma cortisol level was highest ($42.2{\pm}11.318{\mu}g/dL$) after 0 hour, while plasma glucose level was highest ($52.5{\pm}10.61\;mg/dL$) after 1 hour. Plasma cortisol and glucose concentrations required 6 and 2 hours, respectively, to return to pre-exposure levels.

• The Korean Journal of Physiology and Pharmacology
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• 제12권1호
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• pp.7-12
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• 2008

OLETF (Otsuka Long-Evans Tokushima Fatty) rats are characterized by obesity-related insulin resistance, which is a phenotype of type 2 diabetes. Sulfonylurea drugs or benzoic acid derivatives as inhibitors of the ATP-sensitive potassium $(K_{ATP})$ channel are commercially available to treat diabetes. The present study compared sulfonylurea drugs (glimepiride and gliclazide) with one of benzoic acid derivatives (repaglinide) in regard to their long-term effect on ameliorating insulin sensitivity in OLETF rats. Each drug was dissolved and fed with drinking water from 29 weeks of age. On high glucose loading at 45 weeks of age, response of blood glucose recovery was the greatest in the group treated with glimepiride. On immunohistochemistry analysis for the Kir6.2 subunit of $K_{ATP}$ channels, insulin receptor ${\beta}$-subunits, and glucose transporters (GLUT) type 2 and 4 in liver, fat and skeletal muscle tissues, the sulfonylurea drugs (glimepiride and gliclazide) were more effective than repaglinide in recovery from their decreased expressions in OLETF rats. From these results, it seems to be plausible that $K_{ATP}$-channel inhibitors containing sulfonylurea moiety may be much more effective in reducing insulin resistance than those with benzoic acid moiety. In contrast to gliclazide, non-tissue selectivity of glimepiride on $K_{ATP}$ channel inhibition may further strengthen an amelioration of insulin sensitivity unless considering other side effects.

• 생명과학회지
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• 제20권9호
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• pp.1345-1352
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• 2010

본 연구에서는 탈진적인 고강도의 운동 후 현미식초 섭취에 의한 2시간에 걸친 회복기의 에너지기질 변화를 분석하고자 7명의 건강한 대학생을 대상으로 3회의 반복적인 윙게이트 테스트를 통하여 피로를 유발하고 15분이 경과되었을 때 현미식초(brown-rice vinegar, BRV) 또는 물(control, CON)을 섭취시켰다. 채혈은 테스트 전, 후, 회복기 30분, 60분, 그리고 120분에 각각 채혈하였으며, 혈중 글루코스, 젖산, 유리지방산, 암모니아, 그리고 코티졸을 분석하였다. 회복 120분이 경과되었을 때, 4회째의 윙게이트 테스트를 실시하여 3회째에 대한 회복율을 계산하였다. 호흡수, 산소포화도, 그리고 심박수는 유의한 변화는 나타나지 않았다. 혈중 글루코스는 회복 120분에 CON이 BRV에 비하여 유의하게 낮았으며, 혈중 젖산과 암모니아 농도는 회복 60분과 120분에 BRV가 유의하게 낮았고, BRV에서 회복 60분과 120분에 유리지방산 농도가 유의하게 높았다. 윙게이트 테스트의 최대파워 회복율과 체중당 최대파워의 회복 율은 BRV에서 유의한 증가가 나타났다. 이러한 결과는 탈진적인 고강도의 운동 후 현미식초의 섭취에 의해 피로의 회복이 촉진되며, 하루에 두 번 혹은 그 이상 개최되는 경기에 있어서도 효과적인 영양보조물로 사용될 수 있다는 점을 시사한다.

• 생명과학회지
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• 제8권3호
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• pp.241-248
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• 1998

In an attempt to investigate the effects of Samultang, Samultang-Mixed-Kamibulhwangumjungkisan extract on the ability of rat to recover from exhaustion after severe exercise. The results are as follows 1. Lactate was found to show remarkable decrease in the rat with administration of Samultang-Mixed-Kamibulhwangumjungkisan group at 120 min (p<0.001). 2. LDH showed elevation in the rat with administration of Samultang-Mixed-Kamibulhwangumjungkisan group 120 min.(p<0.05) 3. Glucose showed elevation in the rat with administration of Samultang-Mixed-Kamibulhwangumjungkisan group at 120 min.(p<0.05) 4. FFA showed decrease in the rat with adminstration of Samultang-Mixed-Kamibulhwangumjungkisan hroup at 120min.(p<0.05) From these results, we recoginized that the effects of Samul-tangmixedKamiBulhwangumjungkisan group is better than Samultang group on the recovery of exercise induced fatigue.