• Food Science and Biotechnology
• /
• v.14 no.2
• /
• pp.255-258
• /
• 2005

The compounds, 1-Linoleic-2-olein (1), stigmasterol (2), 1,4-glucopyranosyl-1',4'-glucopyranosyl-1",4"-glucopyranoside (3), 2',3'-diphosphoryl-1'-propanoxy-${\beta}$-D-glucopyranoside (4), 1-Linoleic-3-olein (5), l-(N,N,N-trimethyl ethyl amino phosphoryl)-2,3-dilinolein ion (6) and glyceryl phosphate (7) were isolated and identified from the Mushroom of Pholiota adiposa for the first time by column chromatography, TLC, UV, IR, $^1H$ and $^{13}C$ NMR, EI-MS, and FAB-MS. The compounds 1 and 2 were found to have weak cytotoxicity against P388 murine leukemia cells. However, the compounds 6 and 7 did not show any cytotoxicity.

• YAKHAK HOEJI
• /
• v.38 no.4
• /
• pp.469-475
• /
• 1994

As the continued studies for Korean cultivated rhubarb, MeOH extract of the leaves was fractionated with ether, ethylacetate, and n-butanol. From the ethyl acetate fraction of MeOH extract, one flavone glycoside, apigenin-8-${\beta}$-D-glucopyranoside(vitexin, $C_{21}H_{20}O_{10}$) and from the n-BuOH fraction of MeOH extract, two flavonol glycosids, kaempferol-3-O-(2,6-di-O-rhamnopyranosyl)-${\beta}$-D-galactopyranoside$(C_{33}H_{40}O_{19})$and quercetin-3-O-rutinoside(rutin, $C_{27}H_{30}O_{16}$) were isolated and identified through the physico-chemical properties and spectroscopic evidences(UV, IR, NMR, Mass) respectively.

• YAKHAK HOEJI
• /
• v.38 no.4
• /
• pp.410-417
• /
• 1994

As one of the studies for Korean cultivated Rhubarb, which has been used stomachic bitter, laxative and purgative, etc, MeOH extract of the leaves was fractionated with ether, ethylacetate. From the ether fraction of MeOH extract, two anthraquinone derivatives, 1,6,8-trihydroxy-3-methyl anthraquinone(emodin, $C_{15}H_{10}O_5$), 1,6,8-trihydroxy-3-hydroxymethyl anthraquinone (citrerosein, $C_{15}H_{10}O_6$) and from the ethyl acetate fraction of MeOH extract, one anthraquinone derivative, emodin-8-${\beta}$-D-glucopyranoside$(C_{21}H_{20}O_{10})$ were isolated and identified through the physico-chemical properties and spectroscopic evidences(UV, IR, NMR, Mass), respectively.

• Archives of Pharmacal Research
• /
• v.21 no.2
• /
• pp.207-211
• /
• 1998

Three prolyl endopeptidase inhibitors were isolated and identified as luteolin, quercetin and ${\beta}$-sitosterol-3-O-${\beta}$-D-glucopyranoside with $IC_{50}$ of 0.17, 0.19 and 27.5 ppm, respectively. The inhibition of two flavonoids were non-competitive with substrate. Twenty authentic flavonoids were tested in order to investigate structure-activity relationship. No significant relationship was found in them, however, catechol moiety of B-ring and 7-OH group in flavonoid skeleton were seemed to be responsible for the stronger activity.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.27 no.1
• /
• pp.99-109
• /
• 2001

Novel glucoconjugates phenolic moiety, 3-(methoxycarbonyl)-4-(hydroxyphenyl)-$\beta$-D-glucopyranoside(4), 3-(methoxyacetyl)-4-(hydroxyphenyl)-$\beta$-D-glucopyranoside(7), 4-(hydroxyphenyl)-$\beta$-D-ribofuranoside(11), were synthesized. In order to investigate their depigmentation effect, inhibitory activity against mushroom tyrosinase and inhibitory activity of melanin synthesis in B16 melanoma cell were evaluated in vitro. Compound 11 showed 92.0$\mu\textrm{g}$/㎖ of tyrosinase inhibitory activity whereas compound 4 and 7 showed very low activity not less than 300$\mu\textrm{g}$/㎖. Inhibitory activities of melanin synthesis in B16 melanoma cell of compound 4, 7, and 11 were 8.7, 15.1, and 36.0%, respectively, at the concentration of 100$\mu\textrm{g}$/㎖. Inhibitory activity of compound 11 was much higher than that of arbutin at the same concentration.

• Natural Product Sciences
• /
• v.3 no.1
• /
• pp.71-74
• /
• 1997

Cell suspension cultures of Solanum mammosum transformed inoculated salicyl alcohol into salicin $(salicyl\;alcohol\;2-0-{\beta}-D-glucopyranoside)$. The highest level of salicin (59.3 mg/flask) in the cells was formed within 3 days after inoculating with salicyl alcohol (50 mg /flask containing 50 ml medium). The glucosylation capability of salicyl alcohol by cell suspension cultures of S. mammosum was relatively higher than that reported previously.

• Korean Journal of Pharmacognosy
• /
• v.25 no.4 s.99
• /
• pp.328-335
• /
• 1994

The ether soluble fraction of the methanol extract from the fruits of Crataegus pinnatifida var. psilosa was applied to silica gel column chromatography, eluted step-gradiently with a mixture of dichloromethane and methanol, repeatedly, and five compounds were isolated. These compounds are identified as ursolic acid(I), corosolic acid(II) and euscapic acid (III), ${\beta}-sitosteryl-3-O-{\beta}-_D-glucopyranoside(IV)$ and hyperin(V).

• Korean Journal of Pharmacognosy
• /
• v.25 no.4 s.99
• /
• pp.336-341
• /
• 1994

For the investigation of medicinal resources in Aconitum species, the studies were carried out to search the less toxic constituents than Aconitum alkaloid in the aerial part of Aconitum jaluense for. album (Ranunculaceae). From the BuOH fraction of MeOH extract, three flavonoid compounds, hyperoside(1), $kaempferol-3-O-{\beta}-_D-galactopyranoside(2)$, $kaempferol-3-O-{\alpha}-_L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-_D-glucopyranoside(3)$ were isolated and identified on the basis of their physico-chemical properties, spectroscopic evidences (UV, IR, NMR and Mass etc.) and comparison with authentics, respectively.

• Korean Journal of Pharmacognosy
• /
• v.30 no.1
• /
• pp.87-91
• /
• 1999

Two carotenoids and four flavonoids were isolated from the whole plant of Artemisia sylvatica Max. Their structures were identified as ${\beta}-carotene$, lutein, luteolin, rutin, $pectolinarigenin-7-O-{\beta}-glucopyranoside$ and luteolin-7-O-neohesperidoside on the basis of spectroscopic means.

• Natural Product Sciences
• /
• v.5 no.3
• /
• pp.124-126
• /
• 1999

A new natural product $stigmast-5-en-3-O-{\beta}-D-glucopyranoside$ tetraacetate (1) along with known compounds $stigmast-5-en-3-O-{\beta}-D-glucopyranoside$ (2), stigmast-5-en-3-yl-cetate (3) and ${\beta}-sitosterol$ (4) have been isolated from the stems of Cuscuta reflexa. Their structures were elucidated on the basis of chemical and spectroscopic evidence.