• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.276-283
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• 2004

Screening was performed to isolate cellulose-producing microorganisms from the Korean traditional fermented persimmon vinegar. The resulting strain, KJ $145^{T}$, was then taxonomically investigated by phenotypic characterization, particularly chemotaxonomic, and by phylogenetic inference based on a 16S rDNA sequence analysis including other related taxa. Strain KJ $145^{T}$ was found to grow rapidly and form pale white colonies with smooth to rough surfaces on a GYC agar. Strain KJ $145^T$ also produced acetate from ethanol, and was tolerable to 10% ethanol in SM medium. In a static culture, a thick cellulose pellicle was produced, and in GYC broth, the strain grew at temperatures ranging from 28 to $40^\circ{C}$ with an optimum pH of 4.0. The genomic DNA G+C content of strain KJ $145^T$ was 61.9 mol%, and the predominant ubiquinone was Q 10 as the major quinone and Q9 as the minor quinone. The major cellular fatty acids were $C_{16:0}$ and the sum in feature 7 ($C_{18:1}$ w9c, w12t and/or w7c). A 16S rRNA-targeted oligonucleotide probe specific for strain KJ $145^T$was constructed, and the phylogenetic position of the new species was derived from a 16S rDNA-based tree. When comparing the 16S rDNA nucleotide sequences, strain KJ $145^T$ was found to be most closely related to G. hansenii LMG $1527^T$ (99.2%), although KJ $145^T$ was still distinct from G. hansenii LMG $l527^T$ and G. xylinus LMG $1515^T$ in certain phenotypic characteristics. Therefore, on the basis of 16S rDNA sequences and taxonomic characteristics, it is proposed that strain KJ $145^T$ should be placed in the genus Gluconacetobacter as a new species, Gluconacetobacter persimmonis sp. nov., under the type-strain KJ $145^T$ (=KCTC =$10175BP^T$=KCCM=$10354^T$).

• 한국식품영양과학회지
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• 제32권7호
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• pp.981-985
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• 2003

G. persimmonis KJ145$^{T}$ 를 사용하여 bacterial cellulose와 식초 동시 생산에 따른 성분변화를 조사하였다. 그 결과 pH는 배양 8일째에 3.22로 감소하는 경향이었으며, 총산은 8일째에 가장 높은 4.66으로 나타났다. Brix는 배양기간 동안 거의 변화가 없었으며, 유리당은 fructose, glucose 및 sucrose로 구성되어 있고 fructose는 배양 종료 후, 10일째에도 거의 감소하지 않고 높게 나타났다. G. Persimmonis KJ145$^{T}$ 균체는 배양 2일부터 급격히 증가하여 4일에 가장 높게 나타났으며, bacterial cellulose는 배양기간이 길수록 증가하는 경향을 나타내었다. 발효액의 유기산은 malic acid, succinic acid, oxalic acid 등이 검출되었으며, acetic acid는 발효 2일째부터 급격히 증가하여 8일째에 가장 높게 생성되었다. 따라서 bacterial cellulose와 초산을 동시에 생산하기 위한 최적배양기간은 8일 정도로 나타났다.