• Journal of Radiation Protection and Research
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• 제37권2호
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• pp.56-62
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• 2012

To determine the biological effects of low-dose-rate radiation ($^{137}Cs$, 2.95 mGy/h) on EL4 lymphoma cells during 24 h, we investigated the expression of genes related to apoptosis, cell cycle arrest, DNA repair, iron transport, and ribonucleotide reductase. EL4 cells were continuously exposed to low-dose-rate radiation (total dose: 70.8 mGy) for 24 h. We analyzed cell proliferation and apoptosis by trypan blue exclusion and flow cytometry, gene expression by real-time PCR, and protein levels with the apoptosis ELISA kit. Apoptosis increased in the Low-dose-rate irradiated cells, but cell number did not differ between non- (Non-IR) and Low-dose-rate irradiated (LDR-IR) cells. In concordance with apoptotic rate, the transcriptional activity of ATM, p53, p21, and Parp was upregulated in the LDR-IR cells. Similarly, Phospho-p53 (Ser15), cleaved caspase 3 (Asp175), and cleaved Parp (Asp214) expression was upregulated in the LDR-IR cells. No difference was observed in the mRNA expression of DNA repair-related genes (Msh2, Msh3, Wrn, Lig4, Neil3, ERCC8, and ERCC6) between Non-IR and LDR-IR cells. Interestingly, the mRNA of Trfc was upregulated in the LDR-IR cells. Therefore, we suggest that short-term Low-dose-rate radiation activates apoptosis in EL4 lymphoma cells.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
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• pp.231-236
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• 1999

Accurate ocean surface fluxes with high resolution are critical for understanding a mechanism of global climate. However, it is difficult to derive those fluxes by using ocean observation data because the number of ocean observation data is extremely small and the distribution is inhomogeneous. On the other hand. satellite data are characterized by the high density, the high resolution and the homogeneity. Therefore, it can be considered that we obtain accurate ocean surface by using satellite data. Recently we constructed ocean surface data sets mainly using satellite data. The data set is named by Japanese Ocean Flux data sets with Use of Remote sensing Observations (J-OFURO). Here, we introduce J-OFURO. The data set includes shortwave radiation, longwave radiation, latent heat flux, sensible heat flux, and momentum flux etc. Moreover, sea surface dynamic topography data are included in the data set. Radiation data sets covers western Pacific and eastern Indian Ocean because we use a Japanese geostationally satellite (GMS) to estimate radiation fluxes. On the other hand, turbulent heat fluxes are globally estimated. The constructed data sets are used and shows the effectiveness for many scientific studies.

• 방사선산업학회지
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• 제8권3호
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• pp.169-176
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• 2014

For designing the model of the web program, the demand survey for the technology and information has been performed for the students of the related departments, industrialists and researchers. And, the survey, such as advantages and disadvantages, for the current situations has been examined through comparison and analysis by the establishment type and operational process for the present operating web programs having the similar functions in Korea. The contents and web program for the technology and information system have been also developed by the question investigation and the expert opinions. This system for RI-Biomics has been developed by focusing the convenience for the information provision and the information search as the first constructing direction. Information has been collected by the operator in our institute and making contract with Global Trend Briefing of KISTI in Korea. The information collection in the web program has been designed as the direction regularly provided with RSS. Information has been then analyzed by constructing the expert pool provided from the advisory committee for the technology and information, and using them. The publicity for this web program has been performed by webzines and then it is noted that the publicity programs such as some events should be regularly developed when expanded and advanced to a community in future.

• 한국연소학회지
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• 제4권1호
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• pp.131-139
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• 1999

A Numerical Analysis of NOx production in Hydrogen-Air flame is performed using the quasi-laminar reaction modelling. As results, in low global strain rate region, $U_F/D_F\;{\leq}\;50,000$, the quasi-laminar reaction modelling reproduces the experimentally observed EINOx half power scaling that the ratio of EINOx and flame residence time, $L_f^3(D_F^2U_F)$, is proportional to the square root of global strain rate. Thus, it suggests that turbulence-chemistry interaction has a minor impact on the trend of NOx production in low global strain rate region. However, the quasi-laminar reaction modelling predicts the higher temperature and NOx than experimentally observed. This overprediction may be due to the lack of radiation and quasi-laminar reaction modelling.

• Journal of Radiation Protection and Research
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• 제38권4호
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• pp.166-171
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• 2013

While high-dose ionizing radiation results in long term cellular cytotoxicity, chronic low-dose (<0.2 Gy) of X- or ${\gamma}$-ray irradiation can be beneficial to living organisms by inducing radiation hormesis, stimulating immune function, and adaptive responses. During chronic low-dose-rate radiation (LDR) exposure, whole body of mice is exposed to radiation, however, it remains unclear if LDR causes changes in gene expression of the whole brain. Therefore, we aim to investigate expressed genes (EGs) and signaling pathways specifically regulated by LDR-irradiation ($^{137}Cs$, a cumulative dose of 1.7 Gy for total 100 days) in the whole brain. Using microarray analysis of whole brain RNA extracts harvested from ICR and AKR/J mice after LDR-irradiation, we discovered that two mice strains displayed distinct gene regulation patterns upon LDR-irradiation. In ICR mice, genes involved in ion transport, transition metal ion transport, and developmental cell growth were turned on while, in AKR/J mice, genes involved in sensory perception, cognition, olfactory transduction, G-protein coupled receptor pathways, inflammatory response, proteolysis, and base excision repair were found to be affected by LDR. We validated LDR-sensitive EGs by qPCR and confirmed specific upregulation of S100a7a, Olfr624, and Gm4868 genes in AKR/J mice whole brain. Therefore, our data provide the first report of genetic changes regulated by LDR in the mouse whole brain, which may affect several aspects of brain function.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2001년도 추계학술대회 논문집
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• pp.335-335
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• 2001

Direct-normal, total horizontal and diffuse horizontal irradiance data obtained from a multi-filter rotating shadowband radiometer over seven different wavelength bands (416nm, 151nm, 616nm, 675nm, 870nm and 940nm and the entire spectrum) has been analyzed at Kwangju, South Korea from June 1998 December 2000. The maximum hourly global radiation flux ranged from 0.44 MJ/$m^2$ to 2.68 MJ/$m^2$ at around 11:00-12:00 Hr local time while the maximum hourly diffuse radiation flux ranged from about 0.96 MJ/$m^2$ to 1.37 MJ/$m^2$. The maximum hourly direct irradiance ranged from 1.42 MJ/$m^2$ to 2.92 MJ/$m^2$ from June1998-2000. During the period under consideration, the average monthly global radiation recorded were 13.09 MJ/$m^2$/day, 10.58MJ/$m^2$/day and 9.78MJ/$m^2$/day for years 1998, 1999 and 2000 respectively, while the diffuse irradiance were 6.54 MJ/$m^2$/day, 5.33 MJ/$m^2$/day and 5.14 MJ/$m^2$/day for f998, 1999 and 2000 respectively. The direct irradiance values at the site were 11.63 MJ/$m^2$/day, 8.24MJ/$m^2$/day and 7.75 MJ/$m^2$/day for 1998, f999 and 2000 respectively. It was observed that each of the years has its own unique meteorological parameters that affect the quality and quantity of radiation received for each month. The annual average daily fractions of the diffuse to the global radiation (KD) were 0.51, 0.61 and 0.59 for years 1998, 1999 and 2000 respectively. Analysis indicated that the average daily KD ranged from 0.13 to a maximum value of 0.99 in May for irradiance measured at the broadband channel while the maximum and minimum KD value of 0.91 and 0.23 was recorded at wavelength band 870nm and 940nm in January and September respectively. The lowest average daily clearness index (Kt) value recorded was 0.03 in May (Spring) and the annual average daily clearness indexes are 0.45, 0.34 and 0.35 for years 1998, f999 and 2000 respectively. The frequency of clear days annually at Kwangju is 67.95%, 29.57% and 40.72% for years 1998, 1999 and 2000 respectively.

• 천문학회지
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• 제36권spc1호
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• pp.83-91
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• 2003

There are terrestrial signatures of the solar activity cycle in ice core data (Ram & Stoltz 1999), but the variations in the sun's irradiance over the cycle seem too small to account for the signature (Lean 1997; Goode & Dziembowski 2003). Thus, one would expect that the signature must arise from an indirect effect(s) of solar activity. Such an indirect effect would be expected to manifest itself in the earth's reflectance. Further, the earth's climate depends directly on the albedo. Continuous observations of the earthshine have been carried out from Big Bear Solar Observatory since December 1998, with some more sporadic measurements made during the years 1994 and 1995. We have determined the annual albedos both from our observations and from simulations utilizing the Earth Radiation Budget Experiment (ERBE) scene model and various datasets for the cloud cover, as well as snow and ice cover. With these, we look for inter-annual and longer-term changes in the earth's total reflectance, or Bond albedo. We find that both our observations and simulations indicate that the albedo was significantly higher during 1994-1995 (activity minimum) than for the more recent period covering 1999-2001 (activity maximum). However, the sizes of the changes seem somewhat discrepant. Possible indirect solar influences on the earth's Bond albedo are discussed to emphasize that our earthshine data are already sufficiently precise to detect, if they occur, any meaningful changes in the earth's reflectance. Still greater precision will occur as we expand our single site observations to a global network.

• 한국농공학회논문집
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• 제62권5호
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• pp.63-72
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• 2020

Solar-sharing, which is an agricultural photovoltaic system installing solar panels on the upper part of crop growing field, has especially drawn attention. Because paddy fields for cultivating crops are large flat areas, there have been various attempts to utilize solar energy for solar photovoltaic as well as growth of crops in agriculture. Solar-sharing was first proposed in Japan, and has been actively studied for optimization and practical uses. The domestic climate differs from the climate conditions in which the solar-sharing has been widely studied, therefore, it is required to develop the solar-sharing technology suitable for the domestic climate. In this study, a simulation model was developed to analyze the change of solar radiation resulted from the solar-sharing installation. Monthly solar illumination intensity and the change of illumination intensity according to the various conditions of solar panel installation were simulated. The results of monthly illumination analysis differed by altitude of the sun, which was related to season. In addition, it was analyzed that the monthly illumination decreased by up to 42% due to solar-sharing. Accordingly, it is recommended that solar-sharing should be installed as a way to maximize the efficiency of solar photovoltaic system while minimizing the decrease in solar radiation reaching the crops.

• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.574-581
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• 2011

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.

• The Korean Journal of Ecology
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• 제23권4호
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• pp.293-298
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• 2000

To study the effect of global warming on the growth of plants and plant populations throughout their life cycle under a field-like condition, we constructed a Temperature Gradient Chamber (TGC) in Tsukuba, Japan. The chamber had slender shape : 30 m long. 3 m wide, and 2.5 m high. That satisfactory performance was confirmed by a test throughout all seasons in 1998: the projected global warming condition in the near future was simulated. That is, independent of a great daily or seasonal change in ambient meteorological conditions, air temperatures at the air outlet were warmed 5$^{\circ}C$ higher than those at the ambient (the annual mean was 14.3$^{\circ}C$) with precision of ${\pm}$0.2$^{\circ}C$ (the annual means were 19.2$^{\circ}C$) with a rising rate of approximately 1$^{\circ}C$ every 5 m. This chamber will enable us to study the effects of global warming on growth of plants and plant populations because their abilities to control air temperature are excellent. TGC is expected that it would be utilized for studying the effect of global warming on plant growth under natural weather conditions.