• 한국식품과학회지
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• 제29권6호
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• pp.1327-1329
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• 1997

인삼으로부터 사포닌 화합물의 신속한 추출방법을 모색하기 위하여 waring blonder 와 유기용매를 이용한 새로운 추출방법을 개발하였다. 본 방법은 기존의 인삼 증류추출방법과 비교해볼 때 6개의 주종 사포닌($ginsenoside-Rb_2$, $ginsenoside-Rb_1$, ginsenoside-Rc, ginsenoside-Rd, ginsenoside-Re, $ginsenoside-Rg_1$) 함량이 유사하여 유의성이 있는 것으로 생각된다. 유기용매는 메탄올과 클로로포름을 7:3의 비율로 사용하였을 때 사포닌 화합물이 잘 추출되었다. 5개의 주종사포닌을 합한 전체 사포닌 함량은 본 방법에서는 2.41% 이었고 기존의 방법 에서는 2.54%이었다. 그러나 조사포닌의 함량은 본 방법이 기존의 방법보다 높은 것으로 나타났다.

• 약학회지
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• 제35권5호
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• pp.432-437
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• 1991

A mixture of 20(R)- and 20(S)-ginsenoside Rg$_{3}$ was obtained under mild acidic hydrolysis from protopanaxadiol saponins, ginsenosides Rb$_{1}$, Rb$_{2}$, Rc and Rd. The product was acetylated to give the peracetates, which were further converted into 20(R)-ginsenoside Rg$_{3}$, 20(S)-ginsenoside Rg$_{3}$, 20(R)-ginsenoside Rh$_{2}$ and 20(S)-ginsenoside Rh$_{2}$ by the direct alkaline treatment depending upon two kinds of temperature conditions respectively. The structure and physicochemical properties of a prosapogenin, 20(R)-ginsenoside Rh$_{2}$, were investigated.

• Journal of Ginseng Research
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• 제11권2호
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• pp.118-122
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• 1987

인삼의 재배과정중에 부산물로 산출되는 지상부위의 활용방안을 모색하기 위하여 엽과 경의 반성분, 용매별 엑기스 및 사포닌함량을 근의 함량과 대비 검토하여 다음과 같은 결과를 얻었다. 1. 엽은 총당의 함량이 21.5%로 근보다 낮았으나 조직유질, 조지방질 및 회분은 각각 9.41%, 3.43% 및 6.83%로 높았다. 경은 조직유질이 39.2%로 현저하게 많았고 총당과 조단백질은 22.7% 및 8.54%로 근보다 낮았다. 2. 용매별 추출물의 수율은 추출용매의 극성이 클수록 높았으며 근과 경에 비하여 엽은 methanol 및 ehanol 추출물의 수율이 35.9% 및 27.3%로 현저하게 많았고, acetone 및 ethyl acetate추출물도 5.64% 및 3.52%로 높았으며 그 외의 비극성 용매의 추출물의 수율도 대체로 높았다. 3. 총 조saponin의 수율은 근과 경은 4.78% 및 2.22%였으나 엽은 19.58%로 현저하게 높았다. HPLC에 의한 분석결과 엽에는 ginsenoside-Rg1(3.32%), -Re(3.24%), -Rd(2.32 %), -Rc(0.65%), -Rb2(0.92%), -Rbl(0.29%), and -Rf(0.11%)가 함유되었고, 경에는 ginsenoside-Rgl(0.28%), -Re(0.3%), -Rd(0.05%), -Rf(0.11%)외에 미량의 -Rbl, -Rb2, -Rc가 검출되었다. 특히 엽은 총 saponin과 ginsenoside-Rg1, -Re 및 -Rd외에도 -Rc와 -Rb2의 함량이 높아서 이들 성분의 분리용 시료로 적합함을 알 수 있었다.

• 한국융합학회논문지
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• 제8권12호
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• pp.1-7
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• 2017

본 연구는 환경정화에 주로 사용되는 미세기포를 2년 근 인삼 지속적으로 처리하여 인삼이 성장하면서 변화되는 형태와 ginsenoside 변화를 조사하는 융합적 연구이다. 인삼 재배시 미세기포수를 적용하여 4개월 동안(120일) 재배한 후 인삼의 잎과 뿌리의 부위별 ginsenoside 함량과 조성을 분석하였다. 잎에 일반수를 처리한 결과 protopanaxatriol(PPT) 계열 Re 함량만 월등히 높게 나타났지만 미세기포수를 처리한 결과 protopanaxadiol(PPD) 계열 Rb1, RC, Rb2, Rd 성분도 같이 증가시키는 것을 확인하였다. 특히 Re, Rb1이 다량 증가함으로써 전체적인 total ginsenoside가 증가하는 요인이 되었다. 인삼의 부위별 PD/PT 비율은 미세기포수를 처리한 잎에서는 0.811으로 나타나고 뿌리는 1.28로 나타났다. 이것은 미세기포수 처리가 뿌리에서 ginsenoside의 합성을 유도하여 PD/PT 비율이 1과 가까운 결가를 가져와 유용성분의 증가 및 고른 분포 이루어졌다고 판단된다. 따라서 미세버블수를 사용한 고품질 인삼을 생산하는 재배 방법을 제시하고 인삼의 뿌리와 더불어 잎도 기능성 식품 소재로 활용할 수 있는 가능성을 제시하였다.

• Natural Product Sciences
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• 제14권3호
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• pp.171-176
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• 2008

Column chromatographic separation of 70% EtOH extract of the roots of Korean cultivated-wild ginseng led to the isolation of ten ginsenosides (1 - 10). The isolated compounds were identified as ginsenoside $Rg_1$ (1), ginsenoside Re (2), ginsenoside Rc (3), ginsenoside $Rb_1$ (4), ginsenoside $Rb_2$ (5), ginsenoside Rd (6), ginsenoside $Rg_3$ (7), ginsenoside $F_2$ (8), ginsenoside $Rb_3$ (9), and ginsenoside $Rd_2$ (10) by physicochemical and spectroscopic methods. The compounds (1 - 10) were for the first time isolated from the roots of Korean cultivated-wild ginseng.

• Journal of Ginseng Research
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• 제41권1호
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• pp.36-42
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• 2017

Background: Some differences have been reported in the biotransformation of ginsenosides, probably due to the types of materials used such as ginseng, enzymes, and microorganisms. Moreover, most microorganisms used for transforming ginsenosides do not meet food-grade standards. We investigated the statistical conversion rate of major ginsenosides in ginsenosides model culture during fermentation by lactic acid bacteria (LAB) to estimate possible pathways. Methods: Ginsenosides standard mix was used as a model culture to facilitate clear identification of the metabolic changes. Changes in eight ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, and Rg2) during fermentation with six strains of LAB were investigated. Results: In most cases, the residual ginsenoside level decreased by 5.9-36.8% compared with the initial ginsenoside level. Ginsenosides Rb1, Rb2, Rc, and Re continuously decreased during fermentation. By contrast, Rd was maintained or slightly increased after 1 d of fermentation. Rg1 and Rg2 reached their lowest values after 1-2 d of fermentation, and then began to increase gradually. The conversion of Rd, Rg1, and Rg2 into smaller deglycosylated forms was more rapid than that of Rd from Rb1, Rb2, and Rc, as well as that of Rg1 and Rg2 from Re during the first 2 d of fermentation with LAB. Conclusion: Ginsenosides Rb1, Rb2, Rc, and Re continuously decreased, whereas ginsenosides Rd, Rg1, and Rg2 increased after 1-2 d of fermentation. This study may provide new insights into the metabolism of ginsenosides and can clarify the metabolic changes in ginsenosides biotransformed by LAB.

• Applied Biological Chemistry
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• 제23권4호
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• pp.222-227
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• 1980

고려인삼근(자경종)(高麗人蔘根(紫莖種))의 중심부(형성층내부)(中心部(形成層內部))와 외피십피층(外皮十皮層)에 있는 ginsenoside를 고속액 체크로마토그라피로 분석(分析)하고 경(莖)의 자색정도(紫色程度)와의 관계(關係)를 검토(檢討)하였다. Ginsenoside의 단순상관(單純相關)에 의(依)한 saponin 양상(樣相)의 유사도(類似度)를 경색도군간(莖色度群間) 같은 뿌리 또는 다른 뿌리간(間)에 두부위(部位)에서 비교(比較)한 결과(結果) 경색도(莖色度)는 saponin 양상(樣相)과 관련(關聯)되지 않는 것으로 보였다. Saponin 양상(樣相)은 부위(部位)의 출처(出處)에 관계(關係)없이 서도 다른 부위간(部位間)에 약간 달랐다. 각(各) ginsenoside 함량순위(含量順位)는 표피십피층(表皮十皮層)에서 $Rg_1>Re>Rb_1>Rb_2>Rc>Rg_2{\geq}Rd>Rf$이고 중심부(中心部)에서는 $Rg_1>Re{\geq}Rg_2{\geq}Rb_1{\gg}Rb_2>Rc{\geq}Rd>Rf$였다.

• 한국약용작물학회지
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• 제13권5호
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• pp.254-261
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• 2005

To evaluate the effects of cultivar, environment, age and cultivation times on ginsenoside content among 8 wild populations of American ginseng (Panax quinquefolium), the concentrations of 6 ginsenosides in root were determined at the time of collection (T0) of plants from the wild and 1 year after (T1) transplanting the roots to each of two different forest garden locations. Both location and population had significant effects on root and shoot growth. Overall, ginsenoside Rb1 was most abundant. The second most abundant ginsenoside were Re and Rg1, however the contents of them were not significantly different from each other. Concentrations of Rg1 and Re were inversely related. Ginsenoside Re was influenced by population and location. Ginsenoside Rg1, Rb1, Rc, Rb2 and Rd were influenced by population, location and age. Ginsenoside levels were consistently lower but growth was consistently higher at the more intensively managed garden location.

• Journal of Ginseng Research
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• 제32권4호
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• pp.390-396
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• 2008

For evaluating the quality of ginseng, simple and fast analysis methods are needed to determine the ginsenoside content of the ginseng products. The aim of this study was therefore to optimize conditions for fast analysis of the ginsenosides, the active ingredients in extracts of Korean red ginseng. When tandem HPLC mass spectrometry (HPLC-MS/MS) was used, four forms of ginsenoside, Rb1, Rb2, Rc, and Re, were readily separated in seven minutes using a gradient mobile phase (acetonitrile and water containing acetic acid). This is the shortest separation time reported among the studies of major ginsenoside analysis. When gradient HPLC with UV detection was used, the detection limit was high, but separation of these four ginsenosides required 25 minutes using acetonitrile and water containing formic acid as a mobile phase. HPLC-MS/MS was able to separate ginsenoside Rg1 easily regardless of the mobile phase condition, but the HPLC-UV could not separate Rg1 because acetonitrile concentration in the mobile phase had to be maintained below 20%. Ginsenoside peaks were clearer and had more sensitive detection limits when Korean red ginseng extract was analyzed by the HPLC-MS/MS, but the UV detection was useful for chromatographic fingerprinting of all four major ginsenosides of the extract: Rb1, Rb2, Rc, and Re. Extracts were found to contain 2.17 mg, 1.51 mg, 1.29 mg, and 0.46 mg of ginsenoside Rb1, Rb2, Rc, Re, respectively, per gram weight. The ratios of each ginsenoside in the extracts were 1.0 : 0.7 : 0.6 : 0.2, respectively. Taken together, the results indicate that HPLC-MS/MS spectrometry could be the most useful method for rapid analysis of even small amounts of major ginsenosides, while HPLC with UV detection could also be used for rapid analysis of major ginsenosides and for quality control of ginseng products.

• Journal of Ginseng Research
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• 제40권4호
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• pp.366-374
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• 2016

Background: In this study, we screened and identified an endophyte JG09 having strong biocatalytic activity for ginsenosides from Platycodon grandiflorum, converted ginseng total saponins and ginsenoside monomers, determined the source of minor ginsenosides and the transformation pathways, and calculated the maximum production of minor ginsenosides for the conversion of ginsenoside Rb1 to assess the transformation activity of endophyte JG09. Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis. Results: A total of 32 ${\beta}$-glucosidase-producing endophytes were screened out among the isolated 69 endophytes from P. grandiflorum. An endophyte bacteria JG09 identified as Luteibacter sp. effectively converted protopanaxadiol-type ginsenosides Rb1, Rb2, Rc, Rd into minor ginsenosides F2 and C-K, and converted protopanaxatriol-type ginsenoside Rg1 into minor ginsenoside Rh1. The transformation pathways of major ginsenosides by endophyte JG09 were as follows: $Rb1{\rightarrow}Rd{\rightarrow}F2{\rightarrow}C-K$; $Rb2{\rightarrow}C-O{\rightarrow}C-Y{\rightarrow}C-K$; $Rc{\rightarrow}C-Mc1{\rightarrow}C-Mc{\rightarrow}C-K$; $Rg1{\rightarrow}Rh1$. The maximum production rate of ginsenosides F2 and C-K reached 94.53% and 66.34%, respectively. Conclusion: This is the first report about conversion of major ginsenosides into minor ginsenosides by fermentation with P. grandiflorum endophytes. The results of the study indicate endophyte JG09 would be a potential microbial source for obtaining minor ginsenosides.