• Journal of Ginseng Research
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• 제40권1호
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• pp.28-37
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• 2016

Background: Panax ginseng cannot be cultivated on the same land consecutively for an extended period, and the underlying mechanism regarding microorganisms is still being explored. Methods: Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) and BIO-LOG methods were used to evaluate the microbial genetic and functional diversity associated with the P. ginseng rhizosphere soil in various cultivation ages and modes. Results: The analysis of microbial diversity using PCR-DGGE showed that microbial communities were significantly variable in composition, of which six bacterial phyla and seven fungal classes were detected in P. ginseng soil. Among them, Proteobacteria and Hypocreales dominated. Fusarium oxysporum, a soilborne pathogen, was found in all P. ginseng soil samples except R0. The results from functional diversity suggested that the microbial metabolic diversity of fallow soil abandoned in 2003was the maximum and transplanted soil was higher than direct-seeding soil and the forest soil uncultivated P. ginseng, whereas the increase in cultivation ages in the same mode led to decreases in microbial diversity in P. ginseng soil. Carbohydrates, amino acids, and polymers were the main carbon sources utilized. Furthermore, the microbial diversity index and multivariate comparisons indicated that the augmentation of P. ginseng cultivation ages resulted in decreased bacterial diversity and increased fungal diversity, whereas microbial diversity was improved strikingly in transplanted soil and fallow soil abandoned for at least one decade. Conclusion: The key factors for discontinuous P. ginseng cultivation were the lack of balance in rhizosphere microbial communities and the outbreak of soilborne diseases caused by the accumulation of its root exudates.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.419-429
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• 2022

American ginseng (Panax quinquefolium L.) is a perennial herbaceous plant widely cultivated in China, Korea, the United States, and Japan due to its multifunctional properties. In northwest China, transplanting after 2-3 years has become the main mode of artificial cultivation of American ginseng. However, the effects of the cultivation process on the chemical properties of the soil and bacterial community remain poorly understood. Hence, in the present study, high-throughput sequencing and soil chemical analyses were applied to investigate the differences between bacterial communities and nutrition driver factors in the soil during the cultivation of American ginseng. The responses of soil nutrition in different ecological niches were also determined with the results indicating that the cultivation of American ginseng significantly increased the soluble nutrients in the soil. Moreover, the bacterial diversity fluctuated with cultivation years, and 4-year-old ginseng roots had low bacterial diversity and evenness. In the first two years of cultivation, the bacterial community was more sensitive to soil nutrition compared to the last two years. Proteobacteria, Actinobacteria, Gemmatimonadetes, Acidobacteria, Firmicutes, and Bacteroidetes dominated the bacterial community regardless of the cultivation year and ecological niche. With the increase of cultivation years, the assembly of bacterial communities changed from stochastic to deterministic processes. The high abundance of Sphingobium, Novosphingobium, and Rhizorhabdus enriched in 4-years-old ginseng roots was mainly associated with variations in the available potassium (AK), total phosphorus (TP), total potassium (TK), and organic matter (OM).

• 한국자원식물학회지
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• 제33권6호
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• pp.651-658
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• 2020

The aim of this study was to investigate the comparative growth characteristics and ginenoside contents of wild-simulated ginseng on different years (7 and 13-year-old) by monitoring soil properties of cultivation regions. Plant and soil samples were collected from 6 different cultivation regions. Soil organic matter (OM), total nitrogen (TN) and cation exchangeable capacity (CEC) were significantly higher in 13-year-old wild-simulated ginseng cultivation regions compared to 7-year-old wild-simulated ginseng cultivation regions. Growth characteristics of wild-simulated ginseng had shown significantly higher in 13-year-old wild-simulated ginseng compared to 7-year-old wild-simulated ginseng. Ginsenoside G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 were significantly higher in 13-year-old wild-simulated ginseng than 7-year-old wild-simulated ginseng. According to the results of correlation analysis, soil OM, TN and CEC of the cultivated regions were positively correlated with the growth of wild-simulated ginseng. In addition, the root length of wild-simulated ginseng showed positive correlation with ginsenoside content. Hence, this study was able to investigate the correlation between growth and ginsenoside content of wild-simulated ginseng based on soil characteristics of the cultivation regions.

• 한국약용작물학회지
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• 제26권3호
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• pp.248-253
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• 2018

Background: Dazomet are widely used as soil fumigant to solve soilborne problems, and the degradation intermediates are toxic to nematodes, fungi, bacteria, insects and weeds. Methods and Results: The effects of cultivation of green manure crop, maize before and after soil fumigation on the control of ginseng root rot disease were compared using soil where 6-years-old ginseng was harvested. Fumigant (dazomet) were used for soil fumigation in May and September, respectively. Maize was grown for soil management before and after soil fumigation. After May fumigation, the sowing date of maize was delayed by 15 days and thus its dry weight was decreased significantly. Maize cultivation after May fumigation increased pH but decreased EC, $NO_3$, $P_2O_5$, and K significantly. Maize cultivation after May fumigation decreased fungi population and the ratio of fungi and bacteria. Growth of 2-years-old ginseng was improved and the incidence of ginseng root rot was significantly decreased by maize cultivation after May fumigation. After harvesting 2-years-old ginseng, the population of Cylindrocarpon destructans was not different between treatment of May and September, but Fusarium solani showed a significant increase in September fumigation after maize cultivation. Conclusions: Maize cultivation after soil fumigation was effective in inhibiting ginseng root rot by the amendment of mineral composition and microorganism in fumigated soil.

• 한국토양비료학회지
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• 제49권6호
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• pp.795-806
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• 2016

This study was conducted to improve the continuous techniques for international competitiveness of ginseng industry to Korea-China FTA negotiation and conclusion, and provide the basic information for ginseng industry development of Korea. It was carried out the visiting of the northeastern three provinces (Jilin, Liaoling and Heilongjang) in China for 3-year from 2014 to 2016 and observed the farmers' fields of ginseng cultivation with soil environmental status. The types of ginseng cultivation could be observed in small scales of 0.5~3.0 ha, in middle scales of 4.0~10.0 ha and in large scales of 30~700 ha with the kinds of imhasam, Chinese ginseng, Korean ginseng and western ginseng. Also ginseng was cultivated in newly reclaimed land of forest in two types of direct seeding and transplanting of ginseng seedlings. The field beds of ginseng growing were covered with vinyl films in arch design of 100~130 cm height and vinyl was painted in spraying with blue, green and yellow colours for shading. It was investigated in status of the physico-chemical properties of soils. The physical information on the field soils were silt loam, loam and sandy loam in soil textures, and some plain in low slope, some alluvial fan or local valley in forest of land topography. Soil pH ranged within 5.0~5.2, soil EC was $0.93{\sim}3.78dS\;m^{-1}$, organic matter was $37{\sim}35g\;kg^{-1}$, nitrate nitrogen $63{\sim}490mg\;kg^{-1}$, available $P_2O_5$ $55{\sim}163mg\;kg^{-1}$, and in exchangeable cations, K was 0.30~0.98, Ca was 6.5~14.0, Mg was $1.1{\sim}5.3cmol_c\;kg^{-1}$ in ranges. Farmers used the fertilizer for ginseng cultivation in 10~11 t of compost, $200{\sim}400kg\;ha^{-1}$ of complex fertilizer and $750kg\;ha^{-1}$ of oil cakes. The northeastern three provinces of China can use the newly lands with large areas of ginseng cultivation in soil sickness by continuous cropping. and the soil basic fertility is batter than that of Korean in standard guide of ginseng cultivation soil.

• Journal of Ginseng Research
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• 제41권2호
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• pp.195-200
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• 2017

Background: The natural ratios of carbon (C), nitrogen (N), and sulfur (S) stable isotopes can be varied in some specific living organisms owing to various isotopic fractionation processes in nature. Therefore, the analysis of C, N, and S stable isotope ratios in ginseng can provide a feasible method for determining ginseng authenticity depending on the cultivation land and type of fertilizer. Methods: C, N, and S stable isotope composition in 6-yr-old ginseng roots (Jagyeongjong variety) was measured by isotope ratio mass spectrometry. Results: The type of cultivation land and organic fertilizers affected the C, N, and S stable isotope ratio in ginseng (p < 0.05). The ${\delta}^{15}N_{AIR}$ and ${\delta}^{34}S_{VCDT}$ values in ginseng roots more significantly discriminated the cultivation land and type of organic fertilizers in ginseng cultivation than the ${\delta}^{13}C_{VPDB}$ value. The combination of ${\delta}^{13}C_{VPDB}$, ${\delta}^{15}N_{AIR}$, or ${\delta}^{34}S_{VCDT}$ in ginseng, except the combination ${\delta}^{13}C_{VPDB}-^{34}S_{VCDT}$, showed a better discrimination depending on soil type or fertilizer type. Conclusion: This case study provides preliminary results about the variation of C, N, and S isotope composition in ginseng according to the cultivation soil type and organic fertilizer type. Hence, our findings are potentially applicable to evaluate ginseng authenticity depending on cultivation conditions.

• Journal of Ginseng Research
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• 제43권1호
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• pp.1-9
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• 2019

Background: Korean ginseng is an important cash crop in Asian countries. However, plant yield is reduced by pathogens. Among the Ilyonectria radicicola-species complex, I. mors-panacis is responsible for root-rot and replant failure of ginseng in Asia. The development of new methods to reveal the existence of the pathogen before cultivation is started is essential. Therefore, a quantitative real-time polymerase chain reaction method was developed to detect and quantify the pathogen in ginseng soils. Methods: In this study, a species-specific histone H3 primer set was developed for the quantification of I. mors-panacis. The primer set was used on DNA from other microbes to evaluate its sensitivity and selectivity for I. mors-panacis DNA. Sterilized soil samples artificially infected with the pathogen at different concentrations were used to evaluate the ability of the primer set to detect the pathogen population in the soil DNA. Finally, the pathogen was quantified in many natural soil samples. Results: The designed primer set was found to be sensitive and selective for I. mors-panacis DNA. In artificially infected sterilized soil samples, using quantitative real-time polymerase chain reaction the estimated amount of template was positively correlated with the pathogen concentration in soil samples ($R^2=0.95$), disease severity index ($R^2=0.99$), and colony-forming units ($R^2=0.87$). In natural soils, the pathogen was recorded in most fields producing bad yields at a range of $5.82{\pm}2.35pg/g$ to $892.34{\pm}103.70pg/g$ of soil. Conclusion: According to these results, the proposed primer set is applicable for estimating soil quality before ginseng cultivation. This will contribute to disease management and crop protection in the future.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
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• pp.155-167
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• 1990

Ginseng Is renowned for both its medicinal and herbal uses and successful cultivation of Panax ginseng in Asia and Panax quinquefolium in North America has until recently taken place in the native geographical ranges of the plants. As a consequence of the potential high capital return and anticipated increases in consumer consumption, commercial cultivation of American ginseng now occurs well outside the native range of the plant in North America. In fact, the region of greatest expansion of cultivation is in the semi-arid interior region of British Columbia, Canada. Linked with this expansion is the potential domination of the ginseng industry by agricultural corporations. In the interior of British Columbia, the native deciduous forest environment of eastern North America is simulated with elevated polypropylene shade and a surface covering of straw mulch. The architecture of these environments is designed to permit maximum machinery usage and to minimize labor requirements. Further, with only a four- years growth cycle, plant densities in the gardens are high. In this hot, semi-arid environment, producers believe they have a competitive advantage over other regions in North America because of the low precipitation rates. This helps to minimize atmospheric humidity such that the conditions for fungal disease development are reduced. If soil moisture level become limited, supplemental water can be provided by irrigation. The nature of the radiation and energy balance regimes of the shade and many environments promotes high soil moisture levels. Also, the modified environment redlines soil heating. This can result in an aerial environment for the plant that is stressful and a rooting zone environment that is suloptimal. The challenge of further refining the man modified environment for enhanced plant growth and health still remains. Keywords Panax ginseng, Panax quinquefolium, cultivation, ginseng production.

• 한국약용작물학회지
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• 제17권3호
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• pp.204-209
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• 2009

This study was conducted to assess water movement in paddy-upland rotation soil scheduled for ginseng cultivation through the measurement of infiltration and permeability of soil water. Soil sample was divided with four soil layers. The first soil layer (to 30cm from top soil) was loamy sand, the second and the third soil layers (30$\sim$70 ㎝) were sand, and the fourth (< 120 ㎝) was sandy loam. The soil below 130 ㎝ of fourth soil layer was submerged under water. The shear strength, which represents the resisting power of soil against external force, was 3.1 kPa in the first soil layer. This corresponded to 1/8 of those of another soil layer and this value could result in soil erosion by small amount of rainfall. The rates of infiltration and permeability depending on soil layers were 39.86 cm $hr^{-1}$ in top soil, 2.34 cm $hr^{-1}$ in 30$\sim$70 ㎝ soil layer, 5.23 cm $hr^{-1}$ and 0.18 cm $hr^{-1}$ in 70$\sim$120 ㎝ soil layer, with drain tile, and without drain tile, respectively. We consider that ground water pooled in paddy soil and artificial formation of soil layer could interrupt water canal within soil and affect negatively on water movement. Therefore, we suggest that to drain at 5 m intervals be preferable when it makes soil dressing or soil accumulation to cultivate ginseng in paddy-upland rotation soil to reduce failure risk of ginseng cultivation.

• 한국토양비료학회지
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• 제42권6호
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• pp.430-438
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• 2009

논토양에서 토양의 형태적 및 물리적 특성이 인삼 수량에 미치는 영향을 보면 배수인자의 영향이 가장 컸고, 다음으로 표토의 토성과 유효토심의 영향이 컸다. 반면에 지형, 경사, 석력함량 등이 인삼수량에 미치는 영향은 미미하였다. 밭토양에서는 토양의 형태 및 물리적 특성 중 표토의 토성이 인삼수량에 미치는 영향이 가장 컸으며, 다음으로 유효토심, 석력함량, 배수 순으로 영향이 컸으나 지형, 경사, 경반층 출현깊이 등은 영향이 작았다. 토양의 형태 및 물리적 특성 중 인삼수량에 미치는 영향이 큰 지형, 표토의 토성, 배수등급, 경사, 유효토심, 석력함량 및 경반층 출현 깊이를 재배 적지 기준인자로 선정하였다. 다중회귀 확장기법을 이용하여 논과 밭토양에서 각각의 재배 적지 기준 인자가 인삼 수량에 미치는 기여도를 산출하였다. 인삼 재배 적지 기준 인자가 수량에 미치는 기여도와 적지 기준 인자별 수량지수를 이용하여 재배 적지 기준 인자별로 점수를 부여하고, 적지 기준 인자별 점수를 합산하여 최종 점수에 따라 최적지, 적지, 가능지, 저위생산지로 구분하였다. 최종적으로 토양특성과 기상조건을 중첩하여 논과 밭토양에서의 인삼 재배 적지 기준을 각각 설정하였다. 인삼 재배 적지 기준을 기반으로 하고, 토양도, 기후도 및 지리정보 시스템을 이용하여 주요 인삼 재배단지인 충청북도 음성군에 대하여 인삼 재배 적지 분포도를 작성하였다. 그 결과 필지별 인삼 재배 적지에 대한 정보를 인터넷을 통하여 경작자에게 실시간으로 제공할 수 있는 기반을 조성하였다. 인삼 재배지 조사지역에 대하여 인삼 적지 분포를 분석한 결과 최적지와 적지의 합계가 논토양에서 74.0%, 밭토양에서 88.3.%로 높아 인삼 경작자들이 비교적 적지에 인삼을 재배하고 있음을 확인할 수 있었다.