• Applied Microscopy
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• v.19 no.2
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• pp.74-84
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• 1989

Buffer soluble storage proteins of ginseng seed have been localized by electron microscopy using post-embedding immunocytochemical gold labelling technique. Major components of the storage proteins were revealed to be storage protein-1($SP_{1}$, MW 160,000) and storage protein-2($SP_{2}$, MW 70,000). Both of the storage proteins are glycoproteins. Anti-$SP_{1}$ and anti-$SP_{2}$ from rabbit, against $SP_1$ and $SP_2$, respectively, reacted on sections of ginseng endosperm tissue embedded in Spurr's epoxy resin. The rabbit antibodies were visualized indirectly by reaction with protein A labelled with colloidal gold. Both storage proteins were found to be accumulated together in the same protein bodies, but their relative contents are not equal.

• BMB Reports
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• v.41 no.4
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• pp.338-343
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• 2008

Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration.

• Proceedings of the Ginseng society Conference
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• 1998.06a
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• pp.103-108
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• 1998

The Canadian production of American ginseng (Panax quinquefolius L.) occurs mainly in Ontario, British Columbia and the Atlantic provinces. Although ginseng is a profitable crop, its successful production is dependent on careful consideration of cultural management f include site selection, site preparation, seed selection and handling, shading actors which and mulching, pest and nutritional management, and handling of harvested crops. Diseases of particular concern in Atlantic Canada are root rots caused by Phytopkthora cactorum, Cylindrocarpon destructans and Fusarium sp. Recently two systemic fungicides (metalaxyl and fosetylal) were registered; however, growers in Atlantic Canada have experienced metalaxyl resistance resulting from the reliance on this single compound for the control of Phytophthora sap. Current research being conducted on alternative control of these diseases will be discussed. In weed control research, 2, 4-D, MCPA, clopyralid have continued to show promise for weed contro1 at low rates. In trials to evaluate non-selective herbicides as post-senescence or pre-emergence in ginseng, glyphosate (Round-up) provided control of perennials as well as willowherb and lambsquarters. In phytoxicity trials, ginseng significantly tolerated grass herbicides, including clethodim, rimsulfuron, trakloxydim, nicosulfuron and fenoxyprop. For broadleaf herbicides, significant tolerance was shown for bromoxynil, thifensulfuron methyl, flumetulam/clopyralid, thifensulfuro/tribenuron. Disease and weed management of ginseng in Atlantic Canada will be discussed.

• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.456-466
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• 2002

Wild ginseng production is increasing due to forest recovery for last 30 years. Total number of Symmani (traditional mountain ginseng digger) was 558 in 2001. Provincial distribution of Symmani in 2001 was highest in Kangwon $(32\%),$ next in Choongbook $(21\%)$ and least in Jeonnam $(0.7\%)$ and Kyoungnam $(0.9\%).$ Age distribution of Symmani was $33\%\;for\;fourties,\;32\%$ for fifties and $20\%$ for sixties. There were 8 persons in eighties. Symmanies are still keeping traditional ritual for mountain god serving clothes of colored ribbons and foods. Increased production induced open market system from underground dealing of mountain ginseng. Korea Mountain Ginseng Association established mountain ginseng assessment committee with professional Symmanies in 2001. From September to November in 2001, 987 roots were requested for quality assessment to the committee and 476 roots $(48\%)$ were passed and graded and others were rejected. Highest frequency of rejection was foreign origin. Pass rate was highest $(74\%)$ in Choongnam suggesting best place for quality. Number of collected roots in each province was positively correlated (p=0.05) with number of Symmanies. There are 3 quality groups of mountain ginseng, Heaven (pure natural), Earth (from seeding of wild ginseng) and Man (from seeding or seedling of wild ginseng with slight environmental modification). The relationship between price and age was polynomial in high quality root, Heaven, Earth and seed long head of Man group, and linear in low quality group, seedling long head of Man. The best one in 2001 was 26 g, 124 years old and sold with 109 million won. Quality criteria are age, shape, weight, color and healthy outlook. Fine roots are criteria for health status of roots and taproot is criteria for efficacy and called as medicine barrel. The implication is that ginsenosides have rarely been experienced for efficacy. The quality criteria of cultivated ginseng were originated from those of mountain ginseng. It is unique for mountain ginseng that only fresh one can be on market. Since quality criteria of mountain ginseng must be based on the efficacy experience it is well expected that present criteria might almost be established at the age of Shinnong Materia Medica.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.330-337
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• 2016

Streptomyces sp. A75 and A501 inhibited the mycelial growth of pathogenic Rhizoctonia solani and Pythium sp., which cause the ginseng disease known as damping-off. Three methods were evaluated for the control of these pathogens, using a mixture of the culture broths from Streptomyces sp. A75 and A501. The methods tested were seed dipping with 50-fold diluted broth, drenching of soil with 100-fold diluted broth after sowing, and combined seed dipping and drenching. These methods reduced the incidence of ginseng damping-off caused by R. solani by 81.3%, 84.8%, and 32.2% and that caused by Pythium sp. by 51.0%, 52.1%, and 75.3%, respectively. Based on these results, the combination of seed dipping and soil drenching after sowing using a mixture of the culture broths from Streptomyces sp. A75 and A501 effectively reduced the incidence of damping-off in ginseng.

• Journal of Ginseng Research
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• v.20 no.3
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• pp.325-330
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• 1996

Dynamic parameters of physiology including embryo ratio (ER), embryo length to endosperm length, dry weight ratio between embryo and endosperm (DWR) and respiration rate (RR) in American send (Panax quinquefolium L.) seed were investigated. According to the changes of ER during seed stratification, the duration of embryo afterripening could be divided into three stages as \circled1 embryo slow growth stage (ESGS), ER increased from 7.31% to 20.48% (0.16% day-1): \circled2 embryo rapid growth stage (ERGS), ER increased to 80.98% (0.61% day-1) (75G5+ ERGS=morphological afterripening stage (MP,5)) and \circled3pysiological afterripening stage (PAS), ER Increased to 88.50% (0.094 day-1) only. DWR Increased slowly from 0.20% to 2.76clp (0.016% day-1) in MAS and rapidly to 8.81% (0.061% day-1) in PAS. The RR correlated significantly with ER as well (r=0.8934 > rot, 0.6610). The steep increment of both DWR and RR in PAS indicated that the PAS was not a static stage although the ER was not changed too much. All of these may provide some information for understanding the dormancy mechanisms of American sting seed.

• Journal of Ginseng Research
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• v.5 no.2
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• pp.92-98
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• 1981

This experiment was carried out to inverstigate the characteristics of growth of ginseng Plant on field conditions as affected by seedling weight. Weight of seedling ranged from 0.4 to 1.8g were divided into seven classes at interval of 0.2g. The heavy seedlings showed superiority in stem length, stem diameter, leaf area, number of palmate leaf and leaflets and root weight in 2-,3-, and 4-age as compared to light ones. The heavy seedlings also showed superiority in the ratio of inflorescence appearance in 2-age and seed product in 4-age as compared to light ones, but there were no significant difference in the ratio of missing-plant and the cracking. of root. The differences of growth in field condition as affected by seedling weight were diminished as the age progressed, and the annual growth rates of root of light seedling were high in older plant.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.71-78
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• 1989

The structural changes in the pericarp of Panax ginseng fruit cells are studied during maturation periods. The pericarp can be divided into exocarp, mesocarp and endocarp. The exocarp consists of one layer of epidermal cells which is covered by a thin cuticle and hypodermal cells. A central vacuole and peripheral cytoplasm are observed in the exocarp and mesocarp. Also, irregular wall arrangement are observed during the differentiation. The endocarp is clearly marked off from the others by secondaw wall thickening and lignification. Secretory materials produced by the Golgi complex and rough endoplasmic reticulum vesicles appear to accumulated in the cell wall. These secretory materials are considered major components of the seed coat during the differentiation.

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.154-155
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• 2011

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.94-95
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• 1988