• Title/Summary/Keyword: Ginseng product

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Isolation and Characterization of A Thiodiglycol-Degrading Cupravidus sp. (Thiodiglycol를 분해하는 Cupriavidus sp.의 분리와 특성)

  • Park, Jong-Deok;Kim, Jee-Cheon;Yoon, Ki-Hong
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.311-316
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    • 2007
  • A Gram-negative bacterium capable of degrading thiodiglycol (TDG), main hydrolysis product of sulfur mustard, was isolated from ginseng field in enrichment medium supplemented with TDG as carbon source. The isolate, WS-32, grew optimally at $30-37^{\circ}C$ and pH 6.0-8.0. It was found to be similar to the genus Cupriavi연 on the basis of 165 rRNA sequence, while its biochemical properties were highly homologous to Alcaligenes faecalis. The cell growth of WS-32 strain was slightly inhibited on LB broth by TDG, but the maximum level of its growth was maintained stably in the presence of TDG. After incubation of inoculated LB medium or uninoculated LB medium containing TDG for 2 days, TDG amount of the culture filtrate was analyzed to decrease noticeably by HPLC. TDG and alcohols were also oxidized by cell-free extract of the isolate with maximum activities at pH 8.0 and $45^{\circ}C$.

Inhibitory Effect of Oriental Herbal Medicines on Tumor Necrosis $Factor-{\alpha}$ Production in Lipopolysaccharide-stimulated RAW264.7 cells

  • Cho, Jae-Youl;Park, Ji-Soo;Kim, Pyung-Su;Chae, Sook-Hee;Yoo, Eun-Sook;Baik, Kyong-Up;Lee, Jong-Soo;Park, Myung-Hwan
    • Natural Product Sciences
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    • v.5 no.1
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    • pp.12-19
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    • 1999
  • Eleven out of 118 herbal medicines which are frequently used in Korean traditional prescriptions for inflammatory diseases exhibited more than 50% of inhibition on $TNF-{\alpha}$ production in LPS-stimulated RAW264.7 cells by their total ethanol extracts with 0.1 mg/ml as a final concentration. The active 11 total extracts were prepared from Angelica koreana, Coptis japonica, Cynanchum paniculatum, Magnolia frgesii, Magnolia officinalis, Panax ginseng, Patrinia scabiosaefolia, Pterocarpus santalius, Rhapontica uniflora, Saussurea lappa. Of them, Coptis japonica, Magnolia fargesii and Saussurea lappa also significantly inhibited $TNF-{\alpha}$ production in vivo. These total extracts were sequentially fractionated with n-hexane, methylene chloride, ethyl acetate, n-butanol and water. Among the solvent-fractionated extracts with 0.05 mg/ml as a final concentration, inhibitory effects of Angelica koreana, Magnolia fargesii, Magnolia officinalis, Pterocarpus santalinus, Rhapontica uniflora and Saussurea lappa markedly showed in one or two solvent fractions suggesting that the principles may be concentrated by subfractionation as the main compounds.

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Anti-diabetic Effects of CCCA, CMESS, and Cordycepin from Cordyceps militaris and the Immune Responses in Streptozotocin-induced Diabetic Mice

  • Yun, Yun-Ha;Han, Shin-Ha;Lee, Seung-Jeong;Ko, Sung-Kwon;Lee, Chong-Kil;Ha, Nam-Joo;Kim, Kyung-Jae
    • Natural Product Sciences
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    • v.9 no.4
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    • pp.291-298
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    • 2003
  • Anti-diabetic effect of various fractions of Cordyceps militaris (CM), CCCA (crude cordycepin containing adenosine), CMESS (ethanol soluble supernatant), and cordycepin were evaluated in streptozotocin (STZ) induced diabetic mice, CMESS showed potent inhibitory activity of 34.7% in starch-loaded mice (2 g/kg) while acarbose as a positive standard exhibited 37.8% of inhibition rate. After 3 days administration (50 mg/kg), cordycepin (0.2 mg/kg), and acarbose (10 mg/kg) dramatically reduced blood glucose level (inhibition ratio: 46.9%, 48.4% and 37.5% respectively). CCCA that has high contents of cordycepin (0.656 mg/4 mg) did not influence on reducing blood glucose level. The proliferation of splenocytes and peritoneal macrophages derived from STZ-induced diabetic mice administered samples were evaluated out by addition of mitogens to see the stability of the usage of these herbal medicines. Proliferation of T-lymphocyte was significantly decreased; while NO production was increased more than two fold to STZ control in the cordycepin-administered group. Changes of serum enzyme levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) were also evaluated. Cordycepin administered group was appeared to acarbose. We conclude that CMESS and cordycepin may be useful tools in the control of blood glucose level in diabetes and promising new drug as an anti-hyperglycemic agent without defects of immune responses and other side effects.

Manufacture and Physiological Functionality of Korean Traditional Liquor by using Chamomile (Matricaria chamomile) (캐모마일(Matricaria chamomile)을 이용한 전통 민속주의 제조 및 생리기능성)

  • Lee, Dae-Hyung;Kim, Jae-Ho;Kim, Na-Mi;Lee, Jong-Soo
    • Korean Journal of Food Science and Technology
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    • v.34 no.1
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    • pp.109-113
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    • 2002
  • To develop a new traditional liquor by using chamomile, the condition of alcohol fermentation was investigated by the addition of 5%, 10%, 15% nuruk, and 20% chamomile into mash. The maximum amount of ethanol was produced when 20% chamomile and 15% nuruk were added to cooked rice and fermented by Saccharomyces cerevisiae at $25^{\circ}C$ for 10 days. The acceptability and physiological functionalities of chamomile liquors with different concentrations $(1{\sim}20%)$ of chamomile were compared. The C-5 chamomile liquor which was prepared by adding 5% chamomile into mash showed the best acceptability in the sensory evaluation test and color test, and its fibrinolytic activity, tyrosinase inhibitory activity, and nitrite scavenging activity were good.

Specialized Research on Food Culture of Main Family in Chung-Buk through In-Depth Interview (심층면접을 통한 충북지역 종가(宗家) 음식문화 특성 연구)

  • Kim, Mi-Hye;Han, Jung-In;Chung, Hae-Kyung
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.4
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    • pp.574-593
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    • 2015
  • This research's main objective was to analyze the special qualities of food culture of the Main Family in Chung-Buk through a case study on the diversified types of meals that the Main Family cooked, such as courtesy food, normal food, and seasonal food. The research identifies traditional ingredients of specialties from Chung-Buk through old literature. According to "Sejongsillokjiriji", there are a myriad of ingredients:, including jujube, ginseng, pine mushroom, manna lichen, persimmon, mandarin fish, crab, sweetfish, lacquer, honey, and terrapin, which represent Chung-Buk's unique ingredients. Another reliable source, "Banchandungsok", does not provide a detailed list of traditional foods, but rather a simple list. The majority of food from Chung-Buk, in "Banchandungsok", consist of side dishes like kimchi and salt greens. Eventually, salt greens became diversified. As Chung-Buk is a landlocked province, people often used dried pollack. Kimchi and raw coaker were frequently used as well, and rice cakes encompassed multiple ingredients such as: jujube, mushroom, pine nut, and, dried persimmon. There were distinct differences in the diversity of food and amount of dishes used by the Main Families of Chung-Buk dependent on the ingredients and wealth of each Main Family. Chung-Buk has a landscape full of mountains, so potatoes were abundant and used to make potato Dasik. When cooking process was completed, people placed kelp on to the finished product. Seasonal foods were considered very important, especially on special occasions such as Backjoong, which was a day dedicated to laborers. These foodsprovided a good opportunity for the community to build stronger bonds by sharing rice cake and Yukgaejang with other members of the community. It is apparent that "Jeobbingac" culture, or guest culture, flourished since people in Chung-Buk are traditionally taught to leave spare food for the guest at all times.

Shelf-life prediction of packaged cigarette subjected to different degrees of sealing (봉함도에 따른 포장담배의 저장수명 예측)

  • Keun-hoi Lee;young-hoh Kim;young-taek Lee;Kwang-soo Rhim;yong-tae Kim
    • Journal of the Korean Society of Tobacco Science
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    • v.12 no.2
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    • pp.59-65
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    • 1990
  • In order to predict the shelf-life of cigarettes packaged in typical flexible film under conditions of various temperature, relative humidity and sealing degree, a computer iterative technique was used. Although there were some significant differences at initial equilibrium relative humidity(55%), the experimental results agree fairly well with predictions following the student's t test($\alpha$=0.01) in most cases. Essentially, the higher the storage temperature, the shorter the shelf-life of the cigarette product. The bigger the differences from the initial equilibrium relative humidity, the shorter the storage period of the cigarette. Moisture transfer through the film at relatively high temperature gave higher confidence. The sealing degree, one of the storage parameters, appeared to be a major influencing factor to shelf-life. Slopes($\beta$) of the temp., sealing degree and %rh of the dependent variable to shelf life were 0.49, -0.39 and -0, 28 respectively, when analysed by multiple regression of SPSS software. Below 600m1/min sealing decree of the packed cigarette through the sealing Position at 30mmH20 differential pressure, the shelf-life could be increased by more than six months.

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The radioprotective effects of Bu-Zhong-Yi-Qi-Tang and its major ingredients in irradiated mice (방사선 피폭 마우스에서 보중익기탕 및 구성단미의 효과)

  • Kim, Sung-ho;Oh, Heon;Kim, Se-ra;Jo, Sung-kee;Byun, Myung-woo;Shin, Dong-ho
    • Korean Journal of Veterinary Research
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    • v.40 no.2
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    • pp.221-228
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    • 2000
  • We performed this study to determine the effect of Bu-Zhong-Yi-Qi-Tang, as a prescription of traditional Oriental medicine, and its major ingredients on jejunal crypt survival, endogenous spleen colony formation, apopotosis in jejunal crypt cells, lethality and hematological change of mice irradiated with high and low dose of Y-radiation. Bu-Zhong-Yi-Qi-Tang administration before irradiation protected the jejunal crypts (p<0.0001), increased the formation of endogenous spleen colony (p<0.05) and reduced the frequency of radiation-induced apoptosis (p<0.05). The survival rate and mean survival time of the groups treated with Bu-Zhong-Yi-Qi-Tang within 30 days after the treatment were far better than the irradiation control group. In the experiment on the effect of ingredients of Bu-Zhong-Yi-Qi-Tang, the result indicated that the extract of Rensan (Panax ginseng), Danggui (Angelica sinensis), Shengma (Cimicifuga heracleifolia) and Chaihu (Bupleurum falcatnosa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indicated that BU-Zhong-Yi-Qi-Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the protective nature of Bu-Zhong-Yi-Qi-Tang extract and its ingredients.

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The Radioprotective Effect of Sam-Ryung-Baek-Chul-San (San-Ling-Bai-Shu-San) as a Prescriptions of Traditional Chinese Medicine in Irradiated Mice (방사선 피폭 마우스에서 삼령백출산 및 구성단미의 방호효과)

  • Lee, Song-Eun;Oh, Heon;Yang, Jung-Ah;Chung, Chi-Young;Jang, Jong-Sik;Jo, Sung-Kee;Byun, Myung-Woo;Kim, Sung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.2
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    • pp.444-451
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    • 1999
  • We performed this study to determine the radioprotective effect of Sam Ryung Baek Chul San (San Ling Bai Shu San), as a prescription of traditional Oriental medicine, and its major ingredients. Jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells were investigated in irradiated mice with high and low dose of rays. Sam Ryung Baek Chul San administration before irradiation increased the formation of endogenous spleen colony(p<0.05) and reduced the frequency of radiation induced apoptosis(p<0.05). In the experiment on the effect of ingredients of Sam Ryung Baek Chul San, the result indicated that the extracts of Panax ginseng, Poria cocos and Coix lacryma jabi might have the major radioprotective effects. Although the mechanisms of these inhibitory effects remain to be elucidated, these results indicated that Sam Ryung Baek Chul San might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

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Selection of Non-Timber Forest Products for regional specialization -Focused on Gangwon Inland Mountains Area- (지역별 특화를 위한 단기소득임산물 선정 -강원내륙산간권역을 대상으로 -)

  • Kim, Joon-Soon;Lee, Jae-Keun
    • Journal of Korean Society of Forest Science
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    • v.96 no.4
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    • pp.432-437
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    • 2007
  • The purpose of this study was to select regional distinctive Non-Timber Forest Products (NTFP) in Gangwon Inland Mountains Area. It was considered as NTFP Pine mushroom, Raw Oak Mushroom, Chestnut, Pinenut, Acorn, Resin, Cultivated Mountain Ginseng, Fernbrake, Chinese bellflower, Lance asiabell, Angelica, Aster scaber, Sap, Wood Vinegar. Regional weight, item weight and forest products quotient were used for the selection of NTFP. Forest products quotient was an application of Location Quotient that is used Input-Output analysis. As a result, Chestnut was selected in Chuncheon. Raw Oak Mushroom was selected in Wonju and Pyeongchang. It was selected Pinenut in Hongcheon, Lance asiabell in Hoengseong and Hwacheon, Acorn in Cheorwon, Aster scaber in Yanggu and Sap in Inje.

The Processed Radish Extract Melanogenesis in Humans and Induces Anti-Photoaging Effects in Ultraviolet B-Induced Hairless Mouse Model

  • Kim, Hyun-Kyoung
    • International Journal of Advanced Culture Technology
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    • v.7 no.4
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    • pp.125-136
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    • 2019
  • The radish skin and radish greens are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. Melanin is a pigment that gives colour to our skin. But increased production of melanin can turn into benign or malignant tumours. These days due to global warming, the amount of Ultra violet (UVB) rays has been extensively increased with sunlight. Due to this, a phenomenon called exogenous photo aging is widely observed for all skin colour and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases (MMP's) that serves as degradation enzymes for extracellular matrix proteins mainly collagen is increased, causing depletion in collagen and resulting in early wrinkles formation. Therefore in our study we used the murine melanoma cell line B16/F10 to study the melanogenesis inhibition by Heated radish extract (HRE) in vitro and we used HRM-2 hair less mice exposed to artificial UVB for checking the efficacy of Heated radish extract in vivo. Furthermore, we prepared a 3% Heated radish extract (HRE) cream and checked its effects on human skin. Our results have clearly demonstrated that Heated radish extract (HRE) have potently suppressed the tyrosinase activity and melanin production in B16/F10 cells. It had also reduced the expression of components involved in melanin production pathway both transcriptionally and transitionally. In in vivo studies, HRE had potently suppressed the expression of MMP's and reduced the wrinkle formation and inhibited collagen degradation. Moreover, on human skin, ginseng cream increased the resilience, skin moisture and enhanced the skin tone. Therefore in light of these findings, we conclude that HRE is an excellent skin whitening and antiaging product.