• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.988-994
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• 2010

The EtOAc fraction of Lespedeza cyrtobotrya showed mushroom tyrosinase inhibitory and radical scavenging activities. Four active compounds were isolated based on Sephadex LH-20 chromatography and HPLC, and the structures were elucidated, on the basis of their LC-MS and NMR spectral data, as 2-(2,4-dihydroxyphenyl)-6-hydroxybenzofuran (1), eriodictyol-7-O-glucopyranoside (2), haginin A (3), and dalbergioidin (4), respectively. Compound (1) showed mushroom tyrosinase inhibitory activity with an $IC_{50}$ value of $5.2\;{\mu}M$ and acted as a competitive inhibitor. Furthermore, $37.3\;{\mu}M$ of compound 1 reduced 50% of the melanin content on human melanoma (MNT-1) cells. The radical scavenging activities of compounds 1, 2, 3, and 4 were shown to have $IC_{50}$ values of 11.0, 24.5, 9.0, and $36.5\;{\mu}M$, respectively, in an ABTS system and $IC_{50}$ values of 42.7, 36.0, 37.7, and $61.7\;{\mu}M$, respectively, in a DPPH system. The mushroom tyrosinase inhibitory activity of the EtOAc fraction of Lespedeza cyrtobotrya was contributed by compounds 1, 3, and 4, and its radical scavenging activity was contributed by compounds 1-4.

• Journal of Applied Biological Chemistry
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• 제52권4호
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• pp.221-225
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• 2009

Lithospermum erythrorhizon has been known well as one of traditional medicine for fever reduction, detoxication, and blood circulation improvement. This study was carried out to isolate biological active compounds from roots of Lithospermum erythrorhizon and to investigate their low density lipoprotein (LDL) antioxidant and anticancer activities. The hexane extract of Lithospermi Radix has been separated on silica gel chromatography and a naphthoquinone pigment compound 1 has been isolated. The structure of the compound 1 has been identified by spectroscopic technique, including MS and NMR, as acetylshikonin (1). Acetylshikonin showed significantly inhibitory activity on $Cu^{2+}$-induced human LDL oxidation with $IC_{50}$ value of $8.8\;{\mu}M$ and obvious anticancer effect by inhibiting farnesyl:protein transferase (FPTase) activity with $IC_{50}$ value of $23\;{\mu}M$, which suggested that acetylshikonin might be useful for the treatment of atherosclerosis and cancer.

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2003년도 춘계학술발표회
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• pp.191-200
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• 2003

Gunsan coastal area is one of region increasing pollution problems. One of the most important factors that cause eutrophication is nutrient materials containing nitrogen and phosphorus which stem from excreation of terrestial sources. At this study, the three-dimensional numerical hydrodynamic and ecosystem model, which was developed by Institute for Resources and Environment of Japan, were applied to analyze the processes affecting the eutrophication. The residual currents, which were obtained by integrating the simulated tidal currents over 1 tidal cycle, showed the presence of a typical. Density driven currents were generated westward at surface and eastward at the bottom in Geum estuary area where the fresh waters are flowing into. The ecosystem model was calibrated with the data surveyed in the field of the study area in annual average. The simulated results of DIN were fairly good coincided with the observed values within relative error of 32.39%. correlation coefficient(r) of 0.99. In the case of DIP, the simulated results were fairly good coincided with the observed values within relative error of 24.26%, correlation coefficient (r) of 0.82. The simulations of DIN and DIP concentrations were performed using ecosystem model under the conditions of 20 ∼ 80% pollution load reductions from pollution sources. In study area, concentration of DIN and DIP were reduced to 20∼80% and under 10% in case of the 80% reduction of the input loads from fresh water respectively. But pollution loads from sediment had hardly affected DIN and DIP concentration. For the environment management of coastal areas, in case of Kunsan area, the most important pollution sources affecting eutrophication phenomenon were found to be the input loads from fresh water.

• 여성건강간호학회지
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• 제2권2호
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• pp.270-283
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• 1996

This study was done for the purpose of analyzing the relationship between menopausal symptoms and depression. Data were collected by a questionnaire from November 28 to December 30, 1995. The subjects were 134 women between 40-61 years of age. The instruments used for this study were The Menopausal symptom scale by Neugartom and Depression scale by Zung. The results of the study were as follows. 1. Mean score of menopausal symptoms was 1.59. Mean score orders of experienced symptoms were "Rack pain and joint pain"(2.02), "General weakness"(1.98), "Nervousness"(1.96). The most serious menopausal symptom was psychosomatic symptom.(1.88) Total score orders of experienced symptoms was 27.9 and over 90.3% of women complained menopausal symptom. 2. Mean score orders of depression was 39.13. It was normal range of depression. 85.1% of the women were normal range of depression and 14.2% of women mild depression. 3. The relationship between menopausal symptoms and depression was statistically significant(r=0.5307, p=0.000). When the relationship among three dimensions of menopausal symptoms were explored the psychosomatic symptom(r=0.4090, P=0.000), Physical symptom(r=0.319, P=0.000), Physical symptom(r=0.319, P=0.000) were statistically significant. 4. General characteristic variables were significantly related to the level of menopausal symptoms as follows ; environment of living(F=2.89, p=0.038), religion(F=4.18, P=0.007), times of birth(F=2.66, p=0.043). The analysis of this study have implication for management of middle aged women's health, to solve the nursing problems, and to prevent and relieve climacteric symptoms.

• 약학회지
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• 제52권4호
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• pp.316-321
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• 2008

The hepatoprotection by the methanol extract of Oenanthe javanica DC (water dropwort) (OJME) was investigated in Sprague Dawley rats with inducing liver damage by acetaminophen. After OJME administration for 1 week, the increase of hepatic lipid peroxide level by acetaminophen-induced hepatotoxicity was significantly reduced. In case of phase I microsomal enzyme systems including cytochrome P-450, aminopyrine N-demethylase and aniline hydroxylase, any significant differences between in control and in OJME-pretreated group was observed after acetaminophen treatment. However, the pretreatment of OJME maintained the hepatic glutathione level and the activity of liver cytosolic glutathione S-transferase, which was significantly decreased by the acetaminophen intoxication. Among the glutathione-generating system, glutathione reductase was more responsible for its biosynthesis rather than ${\gamma}-glutamylcystein$ synthetase. OJME itself showed the strong inhibition activity on DPPH radical generation. In conclusion, OJME administration maintains the liver glutathione pool and hepatic glutathione S-transferase activity, in addition with its high anti-oxidative capability, to show hepatoprotective effect from acetaminophen intoxication.

• 한국수자원학회논문집
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• 제38권2호
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• pp.167-177
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• 2005

본 연구는 미국 지질조사국에서 개발된 장기유출모형인 PRMS 모형을 금강유역 내 용담댐유역에 적용함으로써 PRMS 모형의 공간적인 특성과 유출 모의에 대한 효율성을 검토하였다. 공간적인 유출특성을 구체화하기 위하여 소유역구분과 HRU수의 증감에 따른 유출의 변화를 고찰하였다. 그 결과 소유역 구분에 따라, HRU증감에 따라 유역말단부에서 유량의 변화는 실제로 크게 변화하지 않는 것으로 나타났으며, 따라서 PRMS 모형은 준 분포형적인 성질보다는 오히려 집중형의 성질을 갖는 모형임을 알 수 있었다. 반면, 용담댐유역의 지형입력자료를 USGS의 입력 자료형식으로 변환하고 소유역과 HRU를 고정한 후 최적화 과정을 통하여 유역특성의 매개변수를 설정하였고 이를 실측치와 비교하여 국내 유역의 PRMS 모형의 적용성을 검증하였다. 그 결과 실측자료의 정확성이 제고될 경우, PRMS 모형은 장기 유출특성을 잘 모사하는 모형으로 적용이 가능할 것으로 나타났다.

• 설비공학논문집
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• 제16권9호
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• pp.828-837
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• 2004

The present study investigated two-phase flow distribution, phase separation and pressure drop in multi-microchannel tubes under adiabatic condition. The test section consisted of inlet and outlet headers with the inner diameter of 19.4㎜ and 15 parallel microchannel tubes. Each microchannel tube brazed to the inlet and outlet headers and had 8 rectangular ports with the hydraulic diameter of 1.32㎜. The key experimental parameters were orientation of header (horizontal and vertical), flow direction of refrigerant into the inlet header (in-line, parallel and cross flow) and inlet quality (0.1, 0.2 and 0.3). It was found that the orientation of the header had relatively large effect on the flow distribution and phase separation, while the inlet quality didn't affect much on them. The horizontal header showed the better flow distribution and phase separation characteristics than the vertical one. The parallel flow condition with the horizontal header showed the best performance for the flow distribution and phase separation characteristics under the test conditions. Two-phase pressure drops through the microchannel tubes with the horizontal header were higher than those of the microchennel tubes with the vertical header due to gravitational effect.

• Journal of Applied Biological Chemistry
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• 제59권4호
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• pp.313-316
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• 2016

꽃송이 버섯(S. crispa)을 80 % MeOH 수용액으로 추출하고, 추출물을 EtOAc, n-butyl alcohol 및 물 분획으로 나누었다. EtOAc 분획과 n-BuOH 분획에 대해 $SiO_2$ 및 ODS column chromatography를 반복적으로 실시하여 2종의 ergosterol peroxide를 분리, 정제하였다. infrared spectrometry, nuclear magnetic resonance 및 FAB-MS data를 해석, 화합물 1과 2를 ergosterol peroxide와 3-O-${\beta}$-D-glucopyranosyl ergosterol peroxide로 각각 구조동정 하였다. 두 화합물 모두 꽃송이 버섯으로부터는 이번 실험에서 처음으로 분리되었다.

• International Journal of Arrhythmia
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• 제18권2호
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• pp.66-76
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• 2017

Background and Objectives: Although an anterior linear ablation is an effective lesion set in radiofrequency catheter ablation (RFCA) for longstanding persistent atrial fibrillation (L-PeAF), its durability for bidirectional block (BDB) is only about 60% at repeat procedure. We hypothesized that changes in electrocardiogram (ECG) may predict an anterior line block state and the clinical outcome of L-PeAF ablation. Subjects and Methods: We studied 304 L-PeAF patients (77% male, $60{\pm}10yrs$), who consistently underwent RFCA Dallas lesion set (circumferential pulmonary vein isolation, posterior box lesion, and anterior line) protocol with subsequent comparison of pre-procedural and post-procedural P wave axes, and one year follow-up (n=205) sinus rhythm (SR) ECGs. Results: 1. P wave axis shifted inferiorly at immediate post-procedure (p<0.001), and was independently correlated with BDB of anterior line (${\ss}=10.4$, 95% confidence interval [CI] 2.79-17.94, p=0.008). 2. The degree of post-procedural inferior shift of P wave axis did not reflect clinical recurrence within one-year (n=205, p=0.923), potentially due to conduction recovery of an anterior line. However, among 160 patients without clinical recurrence within one-year, P wave axis at one-year ECG was independently associated with very late recurrence of AF after one-year (n=160, hazard ratio [HR] 0.98; 95% CI 0.97-0.99, p=0.001), during $45.6{\pm}16.7$ months of follow-up. 3. Among 22 patients who underwent repeat procedures, P wave axis shift was more significant in patients with maintained BDB of an anterior line than in those without (p=0.015). Conclusion: An inferior shift of P wave axis reflects the achievement and the maintenance of an anterior line BDB, and is associated with better long-term clinical outcome after catheter ablation for L-PeAF based on Dallas lesion set.

• Journal of Applied Biological Chemistry
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• 제55권4호
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• pp.217-220
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• 2012

오가피(Acanthopanax sessiliflorus Seeman) 열매를 실온에서 70% ethanol (EtOH)로 추출하고 이 추출물을 ethyl acetate (EtOAc) 분획, n-butyl alcohol 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel, octadecyl silica gel 및 Sephadex LH-20 column chromatography를 반복 실시하여 2종의 화합물을 분리, 정제하였다. NMR, infrared spectroscopy, 및 electron ionization/mass spectrometry 등의 spectrum을 해석하여, 화합물 1과 화합물 2를 각각 3,5-dihydroxycinnamic acid과 protocatechuic acid 로 구조를 결정하였다. 화합물 1은 오가자에서는 처음으로 분리된 화합물이다. 또한 이 화합물에 대한 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazyl 및 oxygen radical absorbance capacity radical 소거능을 이용한 항산화 활성을 측정하였는데, 모두 vitamin C보다 2배 이상 활성이 높은 것으로 나타났다.