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http://dx.doi.org/10.3839/jabc.2012.034

Anti-oxidant activity of Phenolic Compound Isolated from the Fruits of Acanthopanax sessiliflorus Seeman  

In, Seo-Ji (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Lee, Dae-Young (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA)
Seo, Kyeong-Hwa (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Nam, Tae-Gyu (Department of Food Science and Biotechnology, Kyung Hee University)
Kim, Dae-Ok (Department of Food Science and Biotechnology, Kyung Hee University)
Kim, Geum-Soog (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA)
Noh, Hyung-Jun (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA)
Kim, Gye-Won (Brewing Research Center, Hankyung National University)
Seo, Woo-Duck (Department of Functional Crop, National Institute of Crop Science, RDA)
Kang, Hee-Cheol (GFC Co., Ltd.)
Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.55, no.4, 2012 , pp. 217-220 More about this Journal
Abstract
The fruits of Acanthopanax sessiliflorus Seeman (Araliaceae) were extracted with 70% aqueous ethanol at room temperature. The concentrated extract was partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and $H_2O$, successively. From the EtOAc fraction, two compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. According to the results of physicochemical and spectroscopic data including NMR, mass spectrometry, and infrared spectroscopy, the chemical structures of the compounds were determined as 3,5-dihydroxycinnamic acid (1) and protocatechuic acid (2). Compound 1 was isolated from the fruits of A. sessiliflirus Seeman for the first time. And the compounds were evaluated for the radical scavenging the antioxidant capacity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazy, and oxygen radical absorbance capacity assay.
Keywords
Acanthopanax sessiliflorus; 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt; 3,5-dihydroxycinnamic acid; 1,1-diphenyl-2-picrylhydrazyl; NMR; oxygen radical absorbance capacity; protocatechuic acid;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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1 Brand-Willians W, Cuvelier ME, and Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Technol 28, 25-30.   DOI   ScienceOn
2 Huang D, Ou B, Hampsch-Woodill M, Flanagan J, and Prior RL (2002) Highthroughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 20, 4437-4.
3 Im KR, Kim MJ, Jung TK, and Yoon KS (2008) The inhibitory effects of Acanthopanax sessiliflorum Seeman on melanogenesis. J Soc Cosmet Scientists Korea 34, 149-56.   과학기술학회마을
4 Kim DO, Jeong SW, and Lee CY (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 81, 321-6.   DOI   ScienceOn
5 Lee IC, Bae JS, Kim TW, Kwon OJ, and Kim TH (2011) Polyphenolic constituents from the aerial parts of Thymus quinquecostatus var. japonica collected on Ulleung island. J Korean Soc Appl Biol Chem 54, 811-6.   과학기술학회마을   DOI
6 Lee SH, Kim BK, Cho SH, and Shin KH (2002) Phytochemical constituents from the fruits of Acanthopanax sessiliflorus. Arch Pharm Res 25, 280- 4.   DOI   ScienceOn
7 Lee SH, Lee YS, Jung SH, Ji J, Shin KH, Kim BK, and Kang SS (2003) Antitumor and immunostimulating activities of Acanthopanax sessiliflorus fruits. Nat Prod Sci 9, 112-6.
8 Lee WT (1996) In Lineamenta Florae Korea (vol. 770). Academy Co, Seoul, Korea.
9 Li XC, Wang XZ, Chen, DF, and Chen SZ (2011) Antioxidant activity and mechanism of protocatechuic acid in vitro. Functional Foods in Health and Disease 1, 232-44.
10 Lim SH, Park, YH, Kwon CJ, Ham HJ, Jeong HN, Kim KH et al. (2010) Anti-diabetic and hypoglycemic effect of Eleutherococcus spp. J Korean Soc Food Sci Nutr 39, 1761-8.   과학기술학회마을   DOI   ScienceOn
11 Nawal AM, Shagufta P, Itrat F, Muhammad N, and Ajaz H (2011) Antioxidant, anti-glycation and anti-inflammatory activities of phenolic constituents from Cordia sinensis. Molecules 16, 10214-26.   DOI
12 Ryoo HS, Park SY, Chang SY, and Yook CS (2003) Triterpene components from the leaves of Acanthopanax sessiliflorus Seem. Korean J Pharmacogn 34, 269-73.   과학기술학회마을
13 Song Y, Deng Y, Huang D, Wen J, Liu Z, and Li FM (2012) LC-MS/MS determination and pharmacokinetic study of four lignan components in rat plasma after oral administration of Acanthopanax sessiliflorus extract. J Ethnopharmacol 141, 957-63.   DOI   ScienceOn
14 Song Y, Yang CJ, Yu K, and Li FM (2011) In vivo antithrombotic and antiplatelet activities of a quantified Acanthopanax sessiliflorus fruit extract. Chinese J Natural Medicines 9, 141-5.
15 Yagi S, Chretien F, Duval RD, Fontanay S, Maldini M, Piacente S et al. (2012) Antibacterial activity, cytotoxicity and chemical constituents of Hydnora johannis roots. South Afr J Bot 78, 228-34.   DOI   ScienceOn
16 Yook CS, Lee DH, and Seo YK (1976) A new forma of Acanthpanax sepcies (I). Korean J Pharmacogn 7, 179-90.
17 Yook CS, Lee DH, Seo YK, and Ryu KS (1977) Study on the constituents in the root bark of Acanthopanax sessiliflorum Seemann (II). Korena J Pharmacogn 8, 31-4.   과학기술학회마을
18 Yoshisumi K, Hirano K, Ando H, Hirai Y, Ida Y, Tsuji TJ et al. (2006) Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase. J Agric Food Chem 54, 335-41.   DOI   ScienceOn