• Journal of Plant Biology
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• v.33 no.2
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• pp.141-146
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• 1990

Germination and vegetative growth of Enteromorpha multiramosa Bliding from Pyoson, Cheju Island were investigated in laboratory under various combinations of temperature (5-$25^{\circ}C$) and salinity (8-48$^{\circ}C$). Percent level of germination was relatively high at all combinations of the two factors. The highest value among the combinations was revealed at 15$^{\circ}C$ and 32$\textperthousand$. Dry weight also was fairly high at all levels of combination with maximum value at 2$0^{\circ}C$ and 32$\textperthousand$. Analysis of variance for germination and growth was completed respectively and polynomial prediction models were constructed. F ratio revealed that all factors had a significant effect (p<0.001) on percentage of germination and dry weight, and their interactions also were significant (p<0.001), although the F ratio of interactions was far less than that for either the separate effect of temperature or salinity. Response surface of polynomial equation represented that temperature influenced less than salinity on germination, while it effected remarkably on vegetative growth, so the Enteromorpha multiramosa was kept to visible macrothalli from winter to spring in Cheju Island.

• Korean Journal of Agricultural Science
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• v.19 no.1
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• pp.1-8
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• 1992

This experiment was conducted to improve the germination when we sowed Chinese milk vetch (Astragalus sinicus L. Local vas. Jinjoo) in the up-land.. The results are summerized as follows 1. There is no difference on the germination of Chinese milk vetch from $15^{\circ}C$ to $35^{\circ}C$. Germination ratio is 7l%, speed is 60% and mean of germinating day. is about 4. But at $10^{\circ}C$ germination ratio and speed are decreased as 65%, 8% but mean of germinating day is increased as 8. 2. Chemicals used for improve of germination $KNO_3$, $H_2SO_4$, HCl, NaOCl, NaOH and GA were increased about 6~8% of germination ratio. 3. Germination in response to soil covering up depth, surface sowing showed 68% of germination and not more than 4cm the seed is germinated 60% but not less than 6cm, germination ratio is not more than 50%. 4. Soil water content needed in the germination is proper about 70~80% of field water capacity and the ratio is 65% but not. more than 40% germination ratio is not more than 31%. 5. Germination in response to seed treatment (1 day sbsorption /1 day dry) germination ratio is not decreased when it treated 2 times but not less than 3 times it decreased seriously and not less that: 5 times it decreased not more than 31%. 6. Germination in response to seed largement, small seed is not good for germination, so seeds harvested after the majority of the seed is ripened.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.132-135
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• 2005

The water absorption ratio was not affected by the planting dates. Hannamkong showed higher water absorption ratio than any sprout -soybean varieties. Soybean seeds produced from later planting showed higher germination percentage than those planted May 25. Pungsannamulkong, Eunhakong, Doremikong, and Someongkong showed over $90\%$ germination after two days, while Tawonkong and Hannamkong were lower, The germination ratio test of the soybean seeds at $25^{\circ}C$ is usually for 7 days. But, germination ratio test of seeds used for sprout-soybean is suitable for 3 days at $25^{\circ}C$.

• The Korean Journal of Food And Nutrition
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• v.3 no.1
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• pp.29-34
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• 1990

This study carried out to changes $\alpha$-amylase activity and isozymes in barley during germination in the dark and red light The specific activity of u-amylase was increased during the germination periods in the dark, giving 355.0 and 523.7 units/mg protein at 3 and 5 day, and the activity was increased by the red light up to 48 and 15% at 3 and 5 days of germination, respectively. The ratio of $\alpha$-amylase I and II was approximately 95:5 at both 3 and 5 days of germination in the dark while the different ratio was found by the red light i. e. 60 : 40 and 90 : 10 at 3 and 5 days of germination, respectively.

• Journal of Plant Biology
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• v.22 no.4
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• pp.95-100
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• 1979

As a part of the studies on the regulatory mechanism of gene expression by $GA_{3}$ , the effects of $GA_{3}$ on the protein biosynthesis and phosphorylation in maize seedlings were investigated. 1. The optimum concentration of $GA_{3}$ for the stimulation of the protein biosynthesis was 0.3mM. 2. The protein biosynthesis was remarkably increase by $GA_{3}$ during the germination. The reason for the decrease in the protein biosynthesis by 48hrs. after germination seems to be a staggered gene expression, and/or increases in protease and RNase activities. 3. The ratio of the amount of the newly synthesized protein in germinating seeds treated with $GA_{3}$ to the amount of proteins secreted into the endosperm was similar to that ratio in control. According to this result, it seems that $GA_{3}$ stimulates only the expression of certain definite genes. 4. By the treatment with $GA_{3}$, the rates of biosynthesis and phosphorylation of proteins were increased up to about 1.5 times during germination and 6 times by 72hrs. after germination, respectively. The ratio of the total soluble proteins to the phosphorpoteins considerably increased in the early germination stage (24hrs.) but decreased after 24hrs. According to the above mentioned results, the stimulation of the phosphorylation of proteins of $GA_{3}$ seems to be attributed to the increases in the activities of protein kinases.

• Korean Journal of Agricultural Science
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• v.10 no.1
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• pp.28-60
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• 1983

This study was carried out to define the effects of external factors including temperature, moisture, oxygen and light quality on the germination of sesame seeds and to investigate the change of major chemical constituents of seeds during germination. The results obtained are summarized as follows: 1. The average germination ratio was from 95.8% to 97.2% when it was tested every $5^{\circ}C$ intervals from $20^{\circ}C$ to $35^{\circ}C$ and no significant difference in germination ratio was found within $20^{\circ}C$ to $35^{\circ}C$. But the germination ratio dropped rapidly to 32.2% when seeds were germinated at $15^{\circ}C$ and the coefficient of variation become greater(77%) 2. The days required for germination ranged from 1.16 to 1. 64 at the temperatures of $35^{\circ}C$ to $25^{\circ}C$ and they were 3.07 and 10.4 at the temperatures of $20^{\circ}C$ and $15^{\circ}C$, respectively. 3. Considering the germination ratio and days needed, $15^{\circ}C$ was assumed to be the minimum temperature for germination practically and this temperature is recommended for testing low temperature tolerance of seed germination of sesame cultivars. 4. The varieties shown the highest low temperature tolerance were Shirogoma and Turkey. The next varieties shown some degree of low temperature germination were Suweon #29, Naebok and IS 58. The varieties with 70 to 80% of germination ratio were Maepo, Suweon #14, Kimpo, Moondeok, and Haenam. Among the 90 varieties tested, the varieties with comparatively high degree of low temperature tolerance were about 10%, and 70% of the low temperature tolerant varieties were domestic varieties. 5. At $12^{\circ}C$ the Shirogoma was the only variety which showed over 50% of germination ratio, 71.4% of the varieties showed less than 20% of germination ratio. When the temperature was raised to $27^{\circ}C$ 18 days after placement at $12^{\circ}C$ all the varieties showed over 90% of germination ratio within 2days. 6. The amounts of water imbibition needed for seed germination were 0.48 to 0.62 times of the seed dry weight at $25^{\circ}C$ and were significantly different among sesame cultivars. About 63% of water required for germination was imbibed in 2 hours after placement of seeds under the germination condition. 7. Under saturated moisture condition the average germination ratio was 0.42%. In the soil of which water potential was -0.4bar 64.8% of the seeds germinated and the most adequate soil water potential for sesame seed germination was about -0.4 to -5.5 bar. The germination ratio decreased as the soil water potential declined below -5.5 bar. 8. Six out of 10 varieties were not influenced by 5% of oxygen in air germination chamber, while varieties such as Yecheon, PI 158073, IS 103 and Euisangcheon showed 64 to 91% of germination under the 5% oxygen content. Under anaerobic condition, cotyledones were not emerged but only hypocotyl was emerged and elongated. The germination ratio of IS 103 decreased significantly under anaerobic condition. 9. When the seeds were dried for 24 hours after 12 hours imbibition of water, the seeds of Cheongsong did not lose their germination ability and 27.5% was germinated but Suweon #9 and Early Russian failed to germinate. However, the germination ratio of IS 103 decreased when the seed were dried 24 hours after 4 hours imbibition of water and the germination ability of IS 103 was maintained even though the seeds were dried for 24 hours after 24 hours imbibition of water. 10. During germination, sugar content of sesame seed increased rapidly and activity of ${\alpha}$-amylase increased gradually while starch content decreased significantly. The rates of increase in sugar content and enzyme activity and decrease in starch content were significantly lower at $15^{\circ}C$ compared with those at $25^{\circ}C$. 11. During germination of sesame seeds, lipid content in the seeds dropped rapidly and the activity of alkaline lipase increased significantly at early stage of germination. The rate of decrease in lipid content and increase in emzyme activity was lower at $15^{\circ}C$ than at $25^{\circ}C$. 12. Four out of 6 varieties were not affected in germination by light wave length. But Suweon #8 was inhibited in germination by 600-650nm. and IS 103 by 600 to 650nm and 500 to 550nm of light wave length. Suweon #8 showed high germination ratio under 650 to 760 nm and 500 to 560nm, and IS 103 under 400 to 470nm and complete darkness. 13. The germination ratios increased significantly in the seeds of which 1000 grain weight is heavier. When the seeds were placed at soil 4cm deep, Cheongsong and Early Russian failed to emerge their cotyledones, but Suweon #9 and IS 103 showed 32.5 and 50% cotyledone emergence, respectively. The extracts from sesame plant and soil where the sesame was cultivated previously did not affect in the-germination of sesame seeds. 14. The covering by black or transparent polyethylene films increased germination ratio compared with uncovered seeds. The covering was effective in shortening the days needed for germination and in improving the early seedling growth, number of capsules per plant and grain yield. Difference was not so seizable between the two polyethylene films but the transparent film appeared somewhat more effective than the black one. 15. Simcheon, Cheongsong. Suweon #9. PI 158073 and IS 103 showed lower rate of water absorbtion by seed during germination and Suweon #8, Suweon #26, Orotall and Euisangcheon showed high increase in seed weight after water absorbtion by seed.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.480-487
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• 2006

This study was conducted to improve the germination percent and germination speed by seed priming using polyethylene glycol (PEG) 8,000 solution on Acer mono seed because it has low and irregular germination characteristics. Seed priming was carried out under 0, -0.25, -0.5, -1.0 and -2.0 MPa of water potential at $20^{\circ}C$ for 3 days. Primed seeds showed higher percent germination, germination speed, mean germination time (MGT) and germination performance index (GPI) than unprimed seeds. Especially, primed seed with -2.0 MPa of water potential had the highest germination properties. And primed seeds had a lower dormancy than the unprimed seeds. Relative growth rate and T/R ratio of seedling from primed seeds were measured to survey the effect of seed priming on the seedling vigor as well as seed germination. The seedlings from primed seeds at -2.0 MPa of water potential showed the highest relative growth rates of height and root collar diameter, however, the lowest value in T/R ratio. This study suggested that seeds priming at -2.0 MPa of water potential under $20^{\circ}C$ for 3 days enhanced not only the percent germination, germination speed, MGT and GPI but also the vigor and growth of seedlings in Acer mono.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.2
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• pp.78-84
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• 2005

This study was conducted to investigate the effects of chemical treatments and electric and magnetic field treatments on germination and seedling growth of onion (Allium cepa L.). Germination ratios of the onion seed were not much different among the tested species. However, germination ratio was much higher in species of small and medium size seed than that of large one. Moisture content of the seeds was shown to be more suitable in the range of 10-15% for seed germination compare to that of 20%. The germination ratio of onion seeds was found to be increased by the chemical treatments in the order of GA<$NaNO_3$<$KNO_3$. In particular, the treatments with 0.1 and 0.2% $KNO_3$ increased the germination ratio by 13 and 15% compared to that of the non-treatment. The seeds treated with electric or magnetic field resulted in much higher germination ratio and better initial growth. Germination ratios of 86 and 88% were found in the 10 kV and 4 Gauss treatments, respectively. The length and weight of the seedlings grown for 40 days after transplanting were increased by 23-45% with 8 kV and 12 Gauss treatments compared to those of non-treatment.

• Proceedings of the KAIS Fall Conference
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• 2009.12a
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• pp.100-102
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• 2009

Effect of temperature, pH and soil depth on germination of Dianthus chinensis, Dianthus barbatus, and Perennial pennant were investigated in growth chamber and soil condition at the ratio of 7:3 (natural soil and organic soil) in laboratory condition. the optimum temperature for seed germination was recorded for $20^{\circ}C-\;25^{\circ}C$. Maximum germination was observed for Dianthus barbatus (76%) where as in soil condition Perennial pennant (51%) showed maximum germination at 1 cm soil depth. Similarly, optimum pH for seed germination was at pH 6 in all the species. So in lower pH (at pH4) seed germination was inhibited. Germination of these selected species at different environmental condition help to construct the ecological sound proof wall to mitigate the noise especially in urban areas.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.22 no.1
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• pp.45-51
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• 1977

The studies were carried to know the effects of GA$_3$, Ethrel and $H_2O$$_2$ on dormancy and germination in ginseng seeds. GA$_3$ stimulated the embryo growth and increased dehiscent (Kaekapp) ratio of the seeds for more than Ethrel and $H_2O$$_2$ GA$_3$ not only increased germination ratio but also shortened the period of germination. Ethrel and $H_2O$$_2$ showed no effects on the germination and there were no significant differences among the treatment levels of GA$_3$. The slow germination of ginseng seeds seemed to be mainly due to the dormancy of endosperm or seed coat rather than of embryo.