• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.3
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• pp.107-115
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• 2008

Herbs and plants widely used for the ecological restoration were selected for germination rate analysis under treatment of microorganisms to determine ideal treatment conditions and medium for enhanced germination rate. Albizzia julibrissin, when submerged in a nutrient medium or distilled water, presented a decrease in germination period rather than increase in germination rate. When treated with microorganism culture solution (JM-2) for 24 hours, 90% germination was achieved in two days, which is sufficient evidence to conclude that such treatment accelerates the germination of Albizzia julibrissin. Germination period decreased for Lespedeza cyrtobotrya samples submerged in microorganism solution for 15 and 48 hours, however, increases in germination rates were not observed. Sample treated in the solution for 24 hours had increased germination rate and enhanced germination period. Microorganism solution treatment had a negative effect on germination for Lespedeza cuneata, unlike Lespedeza cyrtobotrya and Albizzia julibrissin. Microorganism treated seeds of Lepsedeza cuneata had a lower germination rate than that of the control with no treatment. However, submerging treatments in a nutrient medium or distilled water for 24 to 48 hours were proven effective with higher germination rates than control sample with no treatment. Herbs and plants widely used for the ecological restoration were selected for germination rate analysis under treatment of microorganisms to determine ideal treatment conditions and medium for enhanced germination rate. Albizzia julibrissin, when submerged in a nutrient medium or distilled water, presented a decrease in germination period rather than increase in germination rate. When treated with microorganism culture solution (JM-2) for 24 hours, 90% germination was achieved in two days, which is sufficient evidence to conclude that such treatment accelerates the germination of Albizzia julibrissin. Germination period decreased for Lespedeza cyrtobotrya samples submerged in microorganism solution for 15 and 48 hours, however, increases in germination rates were not observed. Sample treated in the solution for 24 hours had increased germination rate and enhanced germination period. Microorganism solution treatment had a negative effect on germination for Lespedeza cuneata, unlike Lespedeza cyrtobotrya and Albizzia julibrissin. Microorganism treated seeds of Lepsedeza cuneata had a lower germination rate than that of the control with no treatment. However, submerging treatments in a nutrient medium or distilled water for 24 to 48 hours were proven effective with higher germination rates than control sample with no treatment.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.355-359
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• 2006

This study was carried out to investigate the factors affecting seed germination of Korean native orchids, such as Habenaria radiata, Bletilla striate and Cypripedium macranthum. In general, seeds of H. radiata was effectively germinated on solid medium and the highest germination (29.6%) was achieved on Hyponex solid medium. But shoot growth was better in MS liquid medium containing $100mL\;L^{-1}$ coconut water (MSC medium). Seed germination of B. striata was effectively induced on MS solid medium. However, no seed germination was observed in all liguid medium tested. Seed germination experiments of C. macranthum were performed using 6 kinds of media and seeds harvested 90 days after cross- and self-pollination. Cross-pollinated seeds were germinated on all the media with the best germination ratio of 4.45% on MSC medium. But no germination was observed from self-pollinated seeds.

• Plant Resources
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• v.6 no.2
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• pp.144-148
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• 2003

Germination efficiency, such as days to germination and conditions for the hybrid seeds, was tested after hybrid seeds were sown on the medium. Seeds were germinated from 67 to 126 days after seeding in all cross combinations, and germination condition was different among hybrid combinations. There was big difference on days to germination based on the different media, that is, days to germination in the hyponex medium was shorter than that in the Knudson C medium. Mutants such as MMS and NaN$_3$ were used to cause mutation. Germination occurred with 0.1 % NaN$_3$ and MMS. However, even though germination occurred in other treatments (0.01 % and 0.2% of NaN$_3$ and MMS), brownish phenomenon was intense, or did not proceed and got worse after 4-5 months of seeding. In addition, it was performed to choose appropriate medium for the growth of Cymbidium rhizome whose media adaptability is more different than other orchids. Different concentration of NAA and Kinetin was used. As a result, C. goeringii hybrid showed difference for the concentration of NAA and Kinetin combination.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.132-137
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• 2016

The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing $10g{\cdot}L^{-1}$ sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with $10g{\cdot}L^{-1}$ sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with $10g{\cdot}L^{-1}$ sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

• Journal of Forest and Environmental Science
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• v.38 no.1
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• pp.38-47
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• 2022

Hymenodictyon orixensis (Roxb.) Mabberley (locally known as Bhutum in Bangladesh) is both an ecologically and economically valuable multipurpose tree species for afforestation and reforestation programs in Bangladesh. Seed germination and seedling development study of H. orixense were conducted to find out the response to different growing medium, e.g., polybag (15×10 cm (T₀) and 20×15 cm (T₁)), sand medium in propagator house (T₂), conventional nursery bed (T₃), and root trainer (T₄) in the Nursery. Consequently, germination behavior and seedling morphological parameters of H. orixense were assessed. The results revealed that the sand medium of the propagator house (T₂) provided the highest germination % (58.57±22.30) and the highest germination energy (11.43±2.43) followed by seedlings growing in 20×15 cm polybags (T1) containing forest topsoil and cow-dung at a ratio of 3:1. Except for germination energy, germination values, and germination capacity, other seed biology parameters, particularly imbibition, germination period, germination rate, and plant survival percent in T₁, T₂, T₃, and T₄ were significantly (p<0.05) different from T₀. Each phenotypic parameter of seedlings and dry matter of shoot and root significantly differed from control except root length (p<0.992). Based on this study, Polybags of 20×15 cm size are regarded as the best medium for quality seedling development of H. orixense. The nursery bed (T₃) had the lowest germination performance and developed more inferior quality seedlings. Thereby, 20×15 cm size of polybags with conventional soil and cow-dung media is recommended for maximum germination and to grow the quality seedlings of H. orixense in the Nursery.

• Korean Journal of Plant Resources
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• v.11 no.1
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• pp.106-110
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• 1998

The present study has been undertake to obtain the fundatmental data of optimum germination condition and to establish storage time for artificial pollinationin Codonopsis lancelata pollen. In vitro condition for germination of freshly collected and stored pollen were examined. The optium temperature for germination of fresh pollen was $25^{\circ}C$. The optium sucrose concentration in the medium ranged from 30 to 40 % and optium pH 6.0% for pollen germination. The rate of pollen germination accelerated considerably in the medium with 1% agar. 30% sucrose, and pH 6. C. lanceolata pollen remained viable for 15 days when stored at 5$^{\circ}C$ with silica gel as desiccant.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.47-56
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• 2018

Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

• Journal of Environmental Science International
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• v.12 no.6
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• pp.659-664
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• 2003

The experiment was carried out to investigate the effects of sulfuric acid and nitric acid among the main components of simulated acid rain (SAR) on the growth of vegetative organs and seed germination of Arabidopsis thaliana. The Arabidopsis treated with SAR supplemented with sulfuric and nitric acids, respectively, showed 28% and 30% decrease of shoot and root growth compared to the control plants, and also many necrotic spots on leaf surfaces after SAR treatment were observed. The shoot and root length for plants grown with nitric acid rain was 14% and 17% lower, respectively, compared to the control, whereas those grown with sulfuric acid rain was 24% and 25% lower than control plants. When Arabidopsis seeds were sown in distilled water, germination rate was 100% after 7 days. However, 80% in SAR medium supplemented with sulfuric and nitric acids, 88% in sulfuric acid rain medium and 93% in nitric acid rain medium. The germination abilities of seeds harvested from SAR supplemented with sulfuric and nitric acids, sulfuric acid rain, and nitric acid rain were 73%, 73% and 94%, respectively. Consequently, sulfuric acids showed more inhibitory effects than nitric acids on the growth of vegetative organs as well as germination rates in Arabidopsis.

• Journal of Plant Biotechnology
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• v.2 no.1
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• pp.29-33
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• 2000

To develop an efficient propagation method for yew tree, zygotic embryos were cultured under various conditions. When dissected embryos were cultured on GA$_3$ containing media, the highest germination frequency was observed on WPM medium contaning 1.0 mg/L GA$_3$. For germination of the embryos, two different conditions were compared; culturing embryos with endosperm (Method I), and 2) culturing embryos only (Method II). Maximum germination was achieved in 0.5 mg/L GA$_3$ when embryos with endosperm were cultured on the media. Of the media tested, White and WPM medium were the most suitable on germination of embryos. The abnormality of yew embryos found was observed when it cultured on GA$_3$ or culture media. About 40% of the precociously germinated embryos could be developed into full seedlings. Seedlings contained taxol in high quantity (535 $\mu\textrm{g}$/g dry weight). In vitro techniques will be sewed as a useful tool for the development of transformed root cultures and biosynthesis studies.

• Korean Journal of Plant Resources
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• v.1 no.1
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• pp.88-92
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• 1988

The study was conducted to determine the optimal basal media and the concentration of plant growth regulator for germination of seeds and growth of plantlet from Dendrobium monile. The results obtained were summarized as follows. Germination was similar in light and dark condition, but the growth of plantlet after germination was better under dark than under light condition in several media. Germination was best in Hyponex and Kyoto solution medium among the 9 media tested. The number of roots/shoot was most in the Hyponex medium containing 0,1ppm NAA and 1.Oppm BA.