• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.116-116
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• 2017

Low-temperature is one of the environmental stress factors that affect plant growth and development and consequently limit crop productivity. The control of seed germination under low-temperature is organized by many genes which are called quantitative trait loci (QTLs). High germination rate for low-temperature is an important factor of growing rice. Previously, we identified a major QTL controlling low-temperature germinability in rice using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, 'Hwaseongbyeo (HS)'. A $BC_3F_7$ line (TR5) showed better low-temperature germinability than its recurrent parent. TR5 was crossed with HS to develop a segregating F2:3 populations for the target QTL. Six SSR markers polymorphic between HS and Rufi were used to screen and fine map the qLTG1. The qLTG1 on chromosome 1, which accounted for 55.5% of the total phenotypic variation, confirmed that Rufi allele enhanced the low-temperature germinability. Intervals between markers CRM16 and CRM15, four candidate genes were identified. The identified candidate genes, which are encoded by a protein of unknown function, showed their direct involvement on seed germination at low-temperature. To identify genes targeted by qLTG1, we investigated the expression profiles of these candidate genes and germination behavior of qLTG1 under different stress conditions and compared to HS, Rufi, and TR5 at $13{\pm}2^{\circ}C$ for 3 days after incubation. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. Hence, the QTL for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.4
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• pp.321-324
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• 2001

Tobacco seeds (Nicotiana tabacum L. cv KF109) were primed in the polyethylene glycol 6000(PEG) solution and then stored at 5 and $25^{\circ}C$ under 40, 60 and 80% relative humidity (RH) conditions for six months. The effect of storage temperature and humidity on mean germination time ($T_{50}$), longevity and germination of the primed tobacco seeds were compared. Untreated seeds (control) stored at $5^{\circ}C$ showed high germinability throughout the entire storage period and humidity, and a decline in germinability showed after 6 months at 60% RH and after 3 months at 80% RH when stored at $25^{\circ}C$, Primed seeds retained high germinability until 6 months at 60% RH and 3 months at 80% RH when stored at $5^{\circ}C$ but showed a significant decline in germinability after 3 months at 40% RH, and 1 months at 60% and 80% RH, respectively when stored at $25^{\circ}C$, Primed seeds were completely lost viability when stored at $25^{\circ}C$ under 60% RH for 6 months and under 80% RH for 3 months.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.477-482
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• 2012

Low-temperature germination is one of the major determinants for stable stand establishment in the rice direct seeding method in temperate regions and at high altitude areas. Quantitative trait loci (QTL) controlling low-temperature germinability in rice were identified using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon and the Korean japonica cultivar, 'Hwaseongbyeo'. The germination rate at $15^{\circ}C$ was measured to represent low-temperature germination and used for QTL analysis. The germination rate at $15^{\circ}C$ for 7 days of Oryza rufipogon and Hwaseongbyeo was 93.3 and 28.7%, respectively, and that of progenies ranged from 0 to 48%. A linkage map was constructed using 135 simple sequence repeat (SSR) markers. Five putative QTLs associated with low-temperature germination were detected on chromosomes 1, 3, 4, 10 and 11. The QTL, qltg10 on chromosome 10 accounted for 19.2% of the total phenotypic variation for low-temperature germinability. Four additional QTL, accounted for 10.4 - 15.1% of the total phenotypic variation. The O. rufipogon alleles in all detected QTLs loci increased the low-temperature germination rate. No QTL associated with low temperature germinability has been detected near the qltg10 QTL in this study suggesting that qltg10 is a new QTL. The locus, qltg10 is of particular interest because of its independence from undesirable height and maturity effects. The DNA markers linked to the QTL for low temperature germinability would be useful in selecting lines with enhanced low temperature germinability in rice breeding program.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1630-1630
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• 2001

NIR specroscopy is widely used today as a quantitative technique for predicting the chemical composition of various agricultural product. However there exist few application for seed quality assessment, especially for seed germinability. This study is to show the possibilities of a nondestructive estimation of germinability in radish (Raphanus sativus L) seeds. The experiment carried out on one radish cultivar (Chung Su Gung Jung, Nong Woo Bio Co., Ltd.) harvested in 1993. NIR(Foss Co.) spectral measurements were carried out on the seeds surface of flat side. The seeds after spectral measurements were planted on blotter individually and observed germination. The seeds were characterized to nongermination and germination group, which in turn grouped to normal and abnormal germination and then compared with the NIR spectra. The spectra from these seed groups were compared each other, The result suggested that NIR spectra could be applicable to determine radish seeds germinability.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.37-45
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• 1970

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.192-192
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• 2017

Direct-seeding cultivation of rice is increasing in Asia instead of transplanting system, because of its lower cost and operational simplicity. Low-temperature germinability (LTG) and anaerobic germinability (AG) are important characters for breeding of varieties for wide-spread adoption of direct-seeding cultivation in rice. This study was performed to characterize LTG and AG of seven rice varieties and identify varieties with strong germinability on both low-temperature and anaerobic conditions. The mean germination rate and germination vigor of seven varieties were 51.7% and 6.0 under low-temperature condition, respectively. Among these varieties, Cheongcheongjinmi and Hwanggeumnodeul had the highest germination rate of 80%, indicating that Cheongcheongjinmi and Hwanggeumnodeul have a good LTG. In anaerobic conditions, the germination rate and coleoptile length for all varieties were 47.6% and 3.2 cm, respectively. Of them, the highest germination rate and coleoptile length were observed in Subo and Hopum, respectively, suggesting that these two varieties are tolerant to anaerobic during germination stage. Molecular characterization by SDS-PAGE revealed that the protein patterns differed at 50 kDa, 40 kDa, and 22 kDa between low-temperature and anaerobic conditions. Varieties identified as good LTG or AG in this study may be used for developing new direct-seeding rice cultivars through pyramiding these traits in the breeding program.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.275-279
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• 1984

To develop a long term storage technique of soybean germplasm, fifty lines of soybean seeds were kept in storage room controlled by air-conditioner and dehumidifier at KAERI. The storage room was kept below 25$^{\circ}C$ of temperature and 60% relative humidity. Germinability of stored seeds was evaluated at every three or six months. On the other hand, the newly harvested seeds of the lines were accerelated aging at 40$^{\circ}C$ and 90% of relative humidity, and compared with non-accerelated seeds. The average germinability at the starting time of storage was 97% but the germinability was decreased to 62% at 42 months after storage in the storage room. The germinability seemed to be affected by seed size and seed coat color. The germinability of small seed lot tended to be higher than that of large seed lot, and the germination response in longer term of storage was greater than those of shorter term of storage. The accerelated aging test for 7 days proved to be a useful technique to pretest germinability of soybean seeds for assaying the relative storage potential.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.711-715
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• 1995

This study was carried out to determine the desirable screeing condition for low-temperature germinability of rice seeds. The germination test was performed with fifty rice varieties from three countries, mainly including the recent Korean rice cultivars. The analyzed result of germination test under three temperature conditions(13$^{\circ}C$, 16$^{\circ}C$, $25^{\circ}C$) pointed out that 11 days after seeding at 13$^{\circ}C$ was the most efficient testing condition for low-temperature germinability in terms of mean and variation in germination rate. Highly significant correlations were observed between germination rates of 11 days after seeding at 13$^{\circ}C$ and germination coefficients, average days of germination at 13 for 20 days. Low-temperature germinability were highly positively associated with days to heading or amylose content, but negatively correlated with grain width.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.333-337
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• 1997

Pods and kernels of two peanut(Arachis hypogaea L.) cultivars, a Shinpung type, Shindaekwangtangkong and a Virginia bunch type, Namdaetangkong were harvested periodically after the gynophore earthing in the field condition and their developments were monitored by observation of the changes in size, weight and moisture content. Seed germinability and seedling vigor were tested to set up a method for hastening the generation advancement in breeding procedure. Pods and kernels reached the maximum in size at 30 to 40 days after gynophore earthing and at that time the kernels had one hundred percent germinability and the seedlings sprouted from those kernels exhibited practically acceptable viability enough to grow for the next generation.

• Korean Journal of Agricultural Science
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• v.2 no.1
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• pp.49-59
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• 1975

This study was done to find out the effect of seven widely used herbicides on mycelial growth, productivity and germ inability of sclerotium of Corticiurm sasakii growing on artificial media. The results obtained are as follows: (1) 2,4-D amine inhibited at 5 to 1000ppm mycelial growth and at 2.5 to 1000ppm sclerotia production. At normal field rate, though mycelial growth and sclerotia production were inhibited highly. sclerotial germinability were inhibited slightly. (2) MCP inhibited at 10 to 1000ppm mycelial growth and at 500 to 1000ppm sclerotia production. At normal field rate, though mycelial growth and sclerotia production were inhibited highly, sclerotial germinability were inhibited slightly. (3) DCPA and PCP inhibited highly or perfectly at 2.5 to 1000ppm mycelial growth and sclerotia production. Also sclerotial germinability was inhibited highly at normal field rate. (4) MO inhibited at 125 to 1000ppm mycelial growth and at 10 to 1000ppm sclerotia production. At normal field rate, though mycelial growth was not inhibited, sclerotial germinabitity was inhibited moderately. (5) CP-53619 inhibited at 2.5 to 1000ppm mycelial growth and at 10 to 1000ppm sclerotia production. At normal field rate, though mycelial growth and sclerotia production were inhibited moderately, sclerotial germinability was inhibited little. (6) NPE inhibited at 5 to 1000ppm mycelial growth and at 10 to 1000ppm sclerotia production. At normal field rate, mycelial growth, productivity and germinability of sclerotium were inhibited slightly.