• Title/Summary/Keyword: Geo-Education

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Detection of Geosmin Production Capability Using geoA Gene in Filamentous Cyanobacteria (Nostocales, Oscillatoriales) Strains (geoA 유전자를 이용한 사상형 남조류(Nostocales, Oscillatoriales)의 Geosmin 생성능 검출)

  • Ryu, Hui-Seong;Shin, Ra-Young;Seo, Kyung-Ae;Lee, Jung-Ho;Kim, Kyunghyun
    • Journal of Korean Society on Water Environment
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    • v.34 no.6
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    • pp.661-668
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    • 2018
  • Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was $17,535ngL^{-1}$ and $14,311ngL^{-1}$ respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin.

The Comparative Consideration on the Basic pattern of Geo-go-ri (저고리 원형제도의 비교고찰)

  • 정옥임
    • Journal of the Korean Home Economics Association
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    • v.32 no.1
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    • pp.211-228
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    • 1994
  • We consider the irrational part of the basic pattern drafting of the Geo-Ko-ri so that we want to design it more scientically. So there are problems which is found after we considered with comparison about the existingly basic pattern drafting of Geo-go-ri. (1) there is a difference between the practical and Calculation. (2) The part of the curved line was mad indistinctly There is a difference betweence the calculating expression and calculating value. (4) There are some parts which lack objectivity about the reference size. (5) The calculating method of Collar-width and Neck-Nidth are not formative and rational. These are items that we must consider and study further. Especially if we can make up of the scientific form in consideration of the formativeness focused on "git" I think it will make a great contribution to not only the standardization of the basic pattern of Geo-go-ri and the use of the use of education but also the manufacture of individual clothing the production by Auto CAD. Auto CAD.

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