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http://dx.doi.org/10.15681/KSWE.2018.34.6.661

Detection of Geosmin Production Capability Using geoA Gene in Filamentous Cyanobacteria (Nostocales, Oscillatoriales) Strains  

Ryu, Hui-Seong (Yeongsan River Environment Research Center, National Institute of Environmental Research)
Shin, Ra-Young (Department of Biology Education, Daegu University)
Seo, Kyung-Ae (Natural Environment Research Division, National Institute of Environmental Research)
Lee, Jung-Ho (Department of Biology Education, Daegu University)
Kim, Kyunghyun (Yeongsan River Environment Research Center, National Institute of Environmental Research)
Publication Information
Journal of Korean Society on Water Environment / v.34, no.6, 2018 , pp. 661-668 More about this Journal
Abstract
Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was $17,535ngL^{-1}$ and $14,311ngL^{-1}$ respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin.
Keywords
Dolichospermum; Nakdong River; Odorous metabolites; Off-flavor; Pseudanabaena;
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