• 생명과학회지
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• 제8권4호
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• pp.381-386
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• 1998

This study was designed to observe the effects of the feeding Gentiana scabra Bunge var.buergeri Max. extract on the improvement of the blood glucose, lipids in the serum of streptozotcin(55mg/kg of body weight, I.P. injection) induced hyperglycemic rats (S.D. strain, ) fed the experimental diets for 4 weeks. Concentrations of blood glucose were significantly higher in the streptozotocin administration(I.P.) groups (group 2(streptozotcin+water), 3(streptozocin+Gentiana scabra Bunge extract), 4(streptozotocin+cholesterol+water), 5(streptozotocin+cholesterol+Gentiana scabra Bunge extract) than those in the control group(group 1(water)). Bolld glucose concentrations were rather lower in the group 3(streptozotocin+Gentiana scabra Bunge extract)than in the group 2(streptozotocin+water), and then those of the group 5 were rather lower than in the group 4. There was almost little significance among the groups. concentrations of total cholesterol in serum were lower in the group 5 than those in the group 4, and the group 3 were rather lower than in the group 2. Concentrations of LDL-cholesterol in serum were significantly lower in the group 3 than those 2. In the ratio of HDL-cholesterol concentrations to total cholesterol concentrations, Gentiana scabra Bunge extract administration groups were higher percentage than the other goups. Concentrations of free cholesterol and cholesteryl ester in serum were rather lower in the groups 3,5 than the other groups, and then those of the group were higher percentage than the other groups. concentrations of free cholesterol and cholesteryl ester in serum were rather lower in the groups 3,5 than the groups, and then those of the group 3 were significantly lower than in the group 4. Concentrations of triglyceride and phospholipid in serum were decreased in the group 3 than in the other groups. The activities of aspartate aminotransferase and alanine aminotransferase in serum were increased in the all experimental groups than in the control group. From the above research, the Gentiana scabra Bunge var.buergeri Max. extracts were effective on the improvement of the lipid compositions in serum of streptozotcin induced hyperglycemic and hypercholosterolemic rats.

• 생약학회지
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• 제32권1호통권124호
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• pp.1-5
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• 2001

The Chinese crude drug, 'Yong Dam (龍膽)' which is derived from the root of Gentiana plants of the family Gentianaceae, has been used as remedy for stomachic, intestinal catarrh, convulsion, etc. With regard to the botanical origin of 'Yong Dam', it has been considered to be Gentiana species of Gentianaceae, especially Gentiana scabra, but there has no pharmacognostical confirmation on it. To clarify the botanical origin of 'Yong Dam' from Korea, we studied on the anatomical characteristics of Gentiana species growing wild in Korea i.e. Gentiana scabra var. buergeri, G. uchiyamai, G. triflora, G. axillariflora var. coreana and of 'Yong Dam' from Korea on Korean market. Through our studies, the botanical origin of 'Yong Dam' from Korea was proved to be Gentiana scabra var. buergeri and Gentiana axillariflora var. coreana.

• 대한의생명과학회지
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• 제9권2호
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• pp.85-91
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• 2003

Gentiana scabra var. buergeri (G. scabra) is a herb known to have therapeutic effect in infection diseases. We studied cellular activation and antitoxoplasmosis in macrophages after G. scabra stimulation. Macrophage activation was detected by nitrite production. Macrophages were treated with G. scabra extracted with water or methanol. Maximal nitrite production was detected in macrophages after stimulation of G. scabra extract 0.1 mg/ml. Maximal nitrite concentration was 23.22 0.003 uM/L in macrophages after water extract of G. scabra and was 24.07 1.41 uM/L after methanol extract of G. scabra. Effect of G. scabra in the phagocytic capacity of macrophages was monitored by using PI (percentage of macrophage infected by T gondii) method. The minimum PI (42.5 2.31) was detected in macrophages treated by water extract of G. scabra 0.1 mg/ml before infection of T gondii. We also examined toxoplasmastatic capacity of macrophage using FI (fold increase) method. The minimum FI (4.46 1.16) was shown in macrophages after water extract of G. scabra 0.1 mg/ml pretreatment before infection. Under electron microscope, proliferation of T gondii was inhibited by extract of G. scabra treatment in macrophages and the mitochondrion and lysosomal vacuoles within cells were increased. Taken together, G. scabra extract activates macrophages and induces toxopalsmastatic activity after T gondii infection. It is suggested that G. scabra may be used as a therapeutic drug against toxoplasmosis.

• 생명과학회지
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• 제8권5호
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• pp.532-536
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• 1998

식이성 고지혈증 유발 Sprague Dawley계 숫흰쥐를 사용하여 용담 열수 추출액을 급여하므로써 혈당 및 혈청의 지질 개선 효과를 규명하기 위해 4주간 실험 사육한 결과, 혈당 농도는 대조군인 1군(물)에 비해 2군(콜레스테롤+물) 및 3군(콜레스테롤+용담추출액)이 높게 나타났으며, 콜레스테롤과 용담 추출액을 급여한 3군이 2군(콜레스테롤+물)보다 다소 낮은 경향을 보였지만 혈당 저하효과는 유의성있게 관찰되지 않았다. 혈청중 총콜레스테롤 농도는 2군에 비하여 3군이 유의적으로 낮게 나타났다. LDL-콜레스테롤 농도는 2군에 비해 3군에서 유의성있게 감소하였으며, 총콜레스테롤에 대한 HDL-콜레스테롤 농도비는 용담 추출액 급여군이 높게 나타났다. 혈청 유리 콜레스테롤 및 콜레스테롤 에스테르 농도는 콜레스테롤 급여군인 2군에 비해 용담 추출액 급여군(3군)이 유의성 있게 감소하였다. 중성지질 및 인지질 농도는 3군의 농도가 낮았다. 혈청 AST 및 ALT활성은 대조군에 비하여 여타 실험군에서 증가하는 경향을 보였다. 이상의 결과에서, 용담 열수 추출액이 식이성 고지혈증 흰쥐에 대한 혈청지질 개선효과가 다소나마 있는 것으로 사료된다.

• 생약학회지
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• 제13권1호
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• pp.1-6
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• 1982

Gentiana uchiyamana Nakai is a perennial herb in Gentianaceous plants. From the root of Gentiana uchiyamana Nakai, the bitter secoiridoid glucosides were isolated and identified as gentiopicroside and swertiamarin. A high-performance liquid chromatographic method for quantitative determination of bitter component, i.e. gentiopicroside in the roots of Gentiana scabra and G. uchiyamana Nakai was developed, using a ${\mu}$-Bondapak $C_{18}$ column and methanol-water mobile phases. The content of gentiopicroside was calculated from calibration curve previously prepared using the standard, 3.55% in the roots of Gentiana scabra and 1.58% in Gentiana uchiyamana Nakai.

• 생약학회지
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• 제30권2호
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• pp.173-176
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• 1999

Bioassay-guided fractionation of a $H_2O$ extract of the roots of Gentiana scabra has furnished 5-(hydroxymethyl)-2-furfural (1) as an inhibitory compound for nitric oxide (NO) production in murine macrophage RAW 264.7 cells stimulated with $interferon-{\gamma}$ plus lipopolysaccharide. Compound 1 showed the moderate inhibition of NO production with $IC_{50}$ value of $803\;{\mu}M$.

• 생약학회지
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• 제12권1호
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• pp.23-25
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• 1981

Methanol extracts of the roots of Atractylodes japonica (alba) (Compositae) and Gentiana scabra (Gentianaceae) were further fractionated with n-butanol, chloroform, n-hexane and water. Then each fraction was p.o. administered to an animal model of hepatitis caused by $CCl_4$ intoxication. Measurements of sleeping time of mice induced by hexobarbital administration indicated that n-butanol and chloroform fractions of Atractylodes japonica (alba), and chloroform and n-hexane fractions of Gentiana scabra showed significant liver protective activities. Measurements of the activities of serum transaminase, S-GOT and S-GPT appeared to support these results.

• Archives of Pharmacal Research
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• 제21권4호
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• pp.436-439
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• 1998

In order to find out anti-platelet activating factor (PAF) from natural resources, Korean medicinal plants used for the treatments of peripheral circulation disorders were tested for their possible protective effects on PAF-induced anaphylactic shock. From the above screening, the methanol extract of Gentiana scabra showed a potent antagonistic activity against PAF. Water suspension of the extract was partitioned with $CH_2$$CI_2$ and EtOAc, successively. The EtOAc fraction which showed the highest activity was chromatographed on silica gel to yield 6 fractions. From the fraction which showed higher PAF-antagonistic activity than the other fractions, compound 1 was isolated by recrystallization. On the basis of spectral data, compound 1 was identified as 2-hydroxy-3-methoxybenzoic acid glucose ester. The compound prevented the mice from the PAF-induced death at a dose of 300 $\mu\textrm{g}$/mouse.

• 생명과학회지
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• 제29권12호
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• pp.1345-1350
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• 2019

한약재로 쓰이는 용담(Gentiana scabra)의 열수 추출물(GS-W)을 정상 마우스에 경구 투여할 경우 in vivo 위장관이송률(ITR)이 유의적이고 용량 의존적으로 증가한다는 사실이, 최근 본 연구진에 의해 보고된 바 있다. 이에 본 연구에서는, in vivo 실험적 위장관 운동 기능 저해(GMD) 마우스 모델에서는 GS-W가 ITR에 어떠한 영향을 미치는지 검정하고자 하였다. GS-W는 5 g/kg의 고용량 경구 투여 시에도 정상 마우스에서 급성 독성을 나타내지 않았고, GMD 마우스에서 ITR을 유의적이고 용량 의존적으로 증가시켰으며, 특히 1 g/kg 경구 투여 시의 ITR은 cisapride 투여 시보다 수치 상으로 높았다. 이러한 결과들은, 1990년대까지 임상적으로 널리 처방되었으나 치명적인 부작용으로 인해 2000년 이후 시장에서 철수된 약물인 cisapride를 GS-W가 대체하여, 인간의 다양한 GMD 상황을 예방하거나 그 증상을 완화시킬 수 있는 잠재력이 있음을 시사한다.

• Plant Resources
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• 제8권2호
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• pp.81-86
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• 2005

An efficient plant regeneration system of Gentiana scabra through somatic embryogenesis was established. Leaves and roots of seedlings of Gentiana scabra excised after germination were cultured on MS basal medium with 2,4-D, NAA or BA. Embryogenic callus was obtained on MS medium with 0.5 mg/L 2,4-D alone or 0.1 mg/L 2,4-D combimation with 1.0 mg/L BA after 45 days of culture. These embryogenic calli gave rise to somatic embryos, which subsequently developed into plantlets on MS medium without PGRs. Also, shoots were effectively differentiated from embryogenic callus when root segments were cultured on MS medium supplement with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Shoots were effectively rooted on MS medium without PGRs. In vitro flowers were formed from plantlets cultured on MS medium with $5\%$ sucrose after 60 days of culture.