• Molecules and Cells
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• v.25 no.2
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• pp.163-171
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• 2008

The genus Cynodon comprises ten species. The objective of this study was to evaluate the genetic diversity of Korean bermudagrasses at the morphological, cytological and molecular levels. Morphological parameters, the nuclear DNA content and ploidy levels were observed in 43 bermudagrass ecotypes. AFLP markers were evaluated to define the genetic diversity, and chromosome counts were made to confirm the inferred cytotypes. Nuclear DNA contents were in the ranges 1.42-1.56, 1.94-2.19, 2.54, and 2.77-2.85 pg/2C for the triploid, tetraploid, pentaploid, and hexaploid accessions, respectively. The inferred cytotypes were triploid (2n = 3x = 27), tetraploid (2n = 4x = 36), pentaploid (2n = 5x = 45), and hexaploid (2n = 6x = 54), but the majority of the collections were tetraploid (81%). Mitotic chromosome counts verified the corresponding ploidy levels. The fast growing fine-textured ecotypes had lower ploidy levels, while the pentaploids and hexaploids were coarse types. The genetic similarity ranged from 0.42 to 0.94 with an average of 0.64. UPGMA cluster analysis and principle coordinate analysis separated the ecotypes into 6 distinct groups. The genetic similarity suggests natural hybridization between the different cytotypes, which could be useful resources for future breeding and genetic studies.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.153-163
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• 1998

Genetic relationships of some poplars in the section Leuce, including 5 species and 11 clones of Populus alba${\times}$glandulosa, were investigated on the basis of RAPD marker analysis. Twenty-two of the 88 arbitrary 10-mer primers, showed reproducible amplification in the preliminary experiment with 6 samples, were used for PCR and generated a total of 181 RAPD markers. Genetic relationships among the analyzed samples were tested by two phenetic methods of the UPGMA and the neighbor-joining, which revealed the close genetic relationship between P. glandulosa and P. alba. And the close genetic relationship between P. glandulosa and P. davidiana was ascertained by the principal component analysis. Based on the observation of the close genetic relationship between them, it was deduced that P. glandulosa might be originated by the saltational speciation caused by the hybridization between P. alba and P. davidiana in nature.

• Korean Management Science Review
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• v.24 no.2
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• pp.157-176
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• 2007

Fuzzy cognitive map(FCM) has long been used as an effective way of constructing the human's decision making process explicitly. By taking advantage of this feature, FCM has been extensively used in providing what-if solutions to a wide variety of business decision making problems. In contrast, the goal-seeking analysis mechanism by using the FCM is rarely observed in literature, which remains a research void in the fields of FCM. In this sense, this study proposes a new type of the FCM-based goal-seeking analysis which is based on utilizing the genetic algorithm. Its main recipe lies in the fact that the what-if analysis as well as goal-seeking analysis are enabled very effectively by incorporating the genetic algorithm into the FCM-driven inference process. To prove the empirical validity of the proposed approach, valid questionnaires were gathered from a number of experts on software sales, and analyzed statistically. Results showed that the proposed approach is robust and significant.

• Development and Reproduction
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• v.18 no.2
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• pp.89-98
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• 2014

The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria and M. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotides primer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to the other primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minor fragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the seven oligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~ SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twenty-one Meretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01 and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely related to that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between two white clam population pairs (P<0.05).

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.2
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• pp.170-176
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• 2012

Genetic diversities, population genetic structures and demographic histories of the thread-sail filefish Stephanolepis cirrhifer were investigated by nucleotide sequencing of 336 base pairs of the mitochondrial DNA (mtDNA) control region in 111 individuals collected from six populations in Korean coastal waters. A total of 70 haplotypes were defined by 58 variable nucleotide sites. The neighbor-joining tree of the 70 haplotypes was shallow and did not provide evidence of geographical associations. Expansion of S. cirrhifer populations began approximate 51,000 to 102,000 years before present, correlating with the period of sea level rise since the late Pleistocene glacial maximum. High levels of haplotype diversities ($0.974{\pm}0.029$ to $1.000{\pm}0.076$) and nucleotide diversities (0.014 to 0.019), and low levels of genetic differentiation among populations inferred from pairwise population FST values (-0.007 to 0.107), support an expansion of the S. cirrhifer population. Hierarchical analysis of molecular variance (AMOVA) revealed weak but significant genetic structures among three groups ($F_{CT}$ = 0.028, p<0.05), and no genetic variation within groups (0.53%; $F_{SC}$ = 0.005, p = 0.23). These results may help establish appropriate fishery management strategies for stocks of S. cirrhifer and related species.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.309-313
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• 2008

The insulin-like growth factor binding protein 3 (IGFBP3) has been investigated as a candidate gene for growth promoting effects in beef cattle and a modulator of IGF bioactivity. Previously, we have reported twenty two sequence variants discovered in Korean native cattle (Hanwoo). In this study, we examined the association between gene-specific polymorphisms of IGFBP3 and cold carcass weight (CW) and marbling score (MS) among Korean native cattle. Among twenty two polymorphisms, four common polymorphic sites (-854G>C, -100G>A, +421G>T and +3863C>A) were genotyped in our beef cattle (n = 437). Statistical analysis revealed that one common polymorphism in the promoter region (-854G>C) showed putative associations with MS (p = 0.03). IGFBP3 variation/haplotype information analyzed in this study will provide valuable information into strategies for the production of a commercial line of beef cattle.

• Plant Resources
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• v.1 no.1
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• pp.26-32
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• 1998

This paper describes the variations in eight agronomic traits in three unadapted local landraces and an inbred cultivar of corn. To compare the agronomic traits in field evaluation with molecular marker evaluation the genotypes of the plant introduction were also checked by 4 microsatellite-SSR loci. The variations of the eight agronomic traits were higher in the local landrades than in the inbred line. which was substantiated by the high genetic variation in the landrades with microsatellite-SSR loci. The level of genetic variation was also different between landraces. Since the genetic evaluation can be easily quantified by the analysis of microsatellite-SSR loci. the threshold level of genetic homogeneity in the population for parental lines in breeding program can be determined and the effort of maintaining the landrace population would be alleviated. As an example in our analysis. the entry from Whachon should not need the same number of selfing generations as the other two landraces to get the level of inbred state. Since this line showed lowest intra-genetic variation within the population.

• 전자공학회논문지 IE
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• v.46 no.2
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• pp.7-10
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• 2009

This paper presents the implementation of libraries of hardware modules for genetic algorithm using VHDL. Evolvable hardware refers to hardware that can change its architecture and behavior dynamically and autonomously by interacting with its environment. So, it is especially suited to applications where no hardware specifications can be given in advance. Evolvable hardware is based on the idea of combining reconfigurable hardware device with evolutionary computation, such as genetic algorithm. Because of parallel, no function call overhead and pipelining, a hardware genetic algorithm give speedup over a software genetic algorithm. This paper suggests the hardware genetic algorithm for evolvable embedded system chip. That includes simulation results and analysis for several fitness functions. It can be seen that our design works well for the three examples.

• Journal of Microbiology and Biotechnology
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• v.4 no.3
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• pp.171-175
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• 1994

To find new crystal protein genes, we screened 42 Bacillus thuringiensis strains of serovar standards by Southern hybridization with a cryI-specific probe which was amplified from B. thuringiensis subsp. kurstaki HDl by polymerase chain reaction (PCR). Two strains, B. thuringiensis subsp. entomocidus HD9 and subsp. subtoxicus HD109, generated weak signals under the low-stringency hybridization conditions. Further analysis with Southern hybridization revealed that the two strains contained cryIB genes which are slightly different from those of B. thuringiensis subsp. thuringiensis HD2. These results were confirmed by PCR with cryIB-specific primers followed by the restriction analysis of PCR products.

• Journal of Species Research
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• v.1 no.1
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• pp.100-103
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• 2012

Nutria Myocastor coypus is one of a well known invasive riparian mammal found species around world from North America to Eurasia and Africa. In South Korea, feral nutrias inhabit areas from the Nakdonggang and Namgang (River) to their tributaries and Upo Wetland where they have had devastating effects on environment. Nevertheless, there has been little research about nutrias in Korea. This study is to analyze the genetic structure of the nutria population in the Upo Wetland and identify the origin of the source populations. Twenty individuals from the Upo Wetland were genotyped using 25 microsatellite loci. When compared with another introduced population, that of the Blackwater Nation Wildlife Refuge in U.S., the Upo population contains considerable genetic variations. Tests for Hardy-Weinberg equilibrium and Bayesian clustering analysis suggest the Upo population is genetically structured and has at least two source populations. This preliminary study presents the need for further in-depth studies about this species which should combine genetic and ecological studies.