• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.1-6
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• 2009

Genetic variability is an important component in the ability of populations to adapt in the face of environmental change. Severe human impacts reduced Muzzafarnagri sheep of India from 500,000 in 1972 to 10,989 in 1973-74. Here we report for the first time the effect of this population decline on levels of genetic variability at 13 FAO recommended ovine microsatellite loci and contrast levels of variability to that in a breed from the same geographical region, which differed in numbers, by an order of magnitude (Marwari sheep). Of the 13 loci, 100% were polymorphic in both breeds. A high degree of genetic variation was observed within populations in terms of both allele diversity (number of alleles per locus, >4) and gene diversity (expected heterozygosity, >0.5), which implied that there is still a substantial amount of genetic diversity at the nuclear loci in a declining population. Nevertheless, overall low number of alleles per locus and relatively less abundance of low frequency alleles in Muzzafarnagri sheep suggested that genetic variability has been comparatively reduced in this population. Bottleneck analysis indicated that a genetic bottleneck did not occur during the most recent decline. In addition, we found that the differentiation among populations was moderate ($F_{ST}$= 11.8%). This study on assessment of genetic effects of the population declines in ovines is a step towards identification of genetically impoverished or healthy populations, which could prove to be a useful tool to facilitate conservation planning in this important species of small ruminants.

• 한국독성학회:학술대회논문집
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• 한국독성학회 1998년도 국제심포지움 및 추계학술대회
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• pp.14-20
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• 1998

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.116.2-117
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• 2003

Late blight, caused by Phytophthora infestans, is one of the most destructive disease on potato and tomato cultivation. To analysis genetic diversity P. infeatans isolates were collected from potato and tomato fields in Korea. These pathogens contained both Al and A2 mating type with metalaxyl-resistant and sensitive isolates. Polymorphisms showed base on RAPD (Random Amplified Polymorphic DNA) in both potato and tomato isolates of P. infestans. Cluster analysis showed high level genetic variation in potato isolates of P. infestans than tomato isolates. P. infestans isolates were observed genetic diversity among them but not grouped among isolates related mating type and metalaxyl response. These results exhibited that P. infestans isolates showing genetic difference among them were distributed in Korea.

• 한국동물생명공학회지
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• 제34권3호
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• pp.183-189
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• 2019

The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (H_exp and H_obs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

• BMB Reports
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• 제28권3호
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• pp.216-220
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• 1995

cDNA for human cholesteryl ester transfer protein (CETP), a potent atherogenic plasma protein that redistributes the neutral lipids among lipoproteins, was expressed in recombinant vaccinia virus-infected cells (CV-1). Two insertion vectors regulated by different promoters were constructed. The vectors were introduced into human thymidine kinase-negative ($TK^-$) 1438 cells infected with wild-type vaccinia virus (WR strain). Recombinant viruses were selected with 5-bromodeoxyuridine (BUdR) and X-gal and identified with DNA dot blot analysis (vSC11-CETP and vTM1-CETP). The CETP cDNA insert in the recombinant vaccinia virus genome was identified by Southern blot analysis. Transcription of CETP cDNA in CV-1 cells infected with recombinant vaccinia virus was monitored by Northern blot analysis using the CETP cDNA as a probe. Positive signals were detected at 1.8 kb in cells infected with vSC11-CETP and at 2.3 kb in cells infected with vTM1-CETP. The recombinant vaccinia virus-infected CV-1 cells were shown to produce functional CETP when the culture medium was subjected to the CETP assay.

• Structural Engineering and Mechanics
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• 제23권4호
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• pp.387-407
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• 2006

A Genetic Algorithm (hereinafter GA) based optimum design algorithm and program for plane steel frames with partially restrained connections is presented. The algorithm was incorporated with the refined plastic hinge analysis method, in which geometric nonlinearity was considered by using the stability functions of beam-column members and material nonlinearity was considered by using the gradual stiffness degradation model that included the effects of residual stress, moment redistribution by the occurrence of plastic hinges, partially restrained connections, and the geometric imperfection of members. In the genetic algorithm, a tournament selection method and micro-GAs were employed. The fitness function for the genetic algorithm was expressed as an unconstrained function composed of objective and penalty functions. The objective and penalty functions were expressed, respectively, as the weight of steel frames and the constraint functions which account for the requirements of load-carrying capacity, serviceability, ductility, and construction workability. To verify the appropriateness of the present method, the optimum design results of two plane steel frames with fully and partially restrained connections were compared.

• 대한조선학회논문집
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• 제52권1호
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• pp.1-7
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• 2015

The weight estimation of floating offshore structures such as FPSO, TLP, semi-Submersibles, Floating Offshore Wind Turbines etc. in the preliminary design, is one of direct measures of both construction cost and basic performance. Through both literature investigation and internet search, the weight data of floating offshore structures such as FPSO and TLP was collected. In this study, the weight estimation model with the genetic programming was suggested for FPSO. The weight estimation model using genetic programming was established by fixing the independent variables based on this data. In addition, the correlation analysis was performed to make up for the weak points of genetic programming; it is apt to induce over-fitting when the number of data is relatively smaller than that of independent variables. That is, by reducing the number of variables through the analysis of the correlation between the independent variables, the increasing effect in the number of weight data can be expected. The reliability of the developed weight estimation model was within 2% of error rate.

• Journal of Plant Biotechnology
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• 제46권2호
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• pp.61-70
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• 2019

There is vast genetic diversity of Myanmar Mangoes. This study mainly focused on indigenous thirteen different mango landraces cultivated in central area of Myanmar, Kyauk-se District and their fruit characteristics by 18 descriptors together with genetic relationship among them by 12 SSR markers. Based on the morpho-physical characters, a wide variation among accessions was found. Genetic characterization of thirteen mango genotypes resulted in the detection of 302 scorable polymorphic bands with an average of 4.33 alleles per locus and an average polymorphism information content (PIC) of 0.7. All the genotypes were grouped into two major clusters by UPGMA cluster analysis and a genetic similarity was observed in a range of 61 ~ 85%. This study may somehow contribute insights into the identification of regional mango diversity in Myanmar and would be useful for future mango breeding program.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.122-122
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• 2005

• 한국미생물생명공학회소식지:생물산업
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• 제9권1호
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• pp.8-13
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• 1996