• 한국작물학회지
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• 제41권2호
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• pp.173-177
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• 1996

경상북도 일원에서 수집한 유색콩 146계통을 공시하여 밥밑콩 유전자원의 이용성을 확대하고 유색콩 품종육성의 기초자료로 활용코자 주요 작물학적 특성을 조사하여 얻어진 결과는 다음과 같다. 1. 수집계통은 흑색, 흑색 아주까리형, 선비잡이형, 밤색, 갈색, 갈색 아주까리형, 녹색 등 종피의 형태에 따라7종으로 분류되었으며 이 중 흑색 및 녹색의 종피를 가진 계통이 가장 많았다. 계통별 화색은 자색과 백색으로 나타났으며 이 중 자색을 가진 계,통이 전체 수집종의 90%에 해당하는 132계통이었다. 종실의 종피를 제거한 후 자엽의 색깔은 녹색이 82계통, 황색이 64계통 이었다. 2. 종피형태 별 개화일수, 결실일수 및 생육일수를 조사한 결과 개화일수 및 결실일수의 경우 51∼81일 및 75∼103일의 변이폭을 나타내어 수집계통간 공히 변이폭이 컸던 반면 생육일수의 경우에는 흑색 아주까리계통(123일)을 제외한 모든 계통에서 150∼159일로 비교적 변이폭이 작았다. 3. 100립중의 경우 109이하의 계통으로부터 36g이상의 계통까지 다양하였으나 이들중 흑색종피를 가진 계통중에서도 자엽색이 녹색인 계통들이 황색 계통에 비해 100립중이 무거운 경향을 보였고, 특히 선비잡이형 종피계통의 경우에는 100립중이 36g이상 되는 극대립종이었다. 4. 수집종의 종실형태는 대체로 편타원형이었으며, 길이 : 폭 : 두께의 비율은 평균1 : 0.87 : 0.69였고, 100립중과 종실의 형태적 특성간의 상관관계는 길이, 폭, 두께 모두가 고도로 유의한 정의 상관을 나타내었다.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.17-22
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• 2008

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also $F_2$ populations derived from the cross between(Jasmine 85 aromatic$\times$Nipponbare non-aromatic) and(Jasmine 85$\times$Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the $F_2$ populations was tested by $x^2$ analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

• 자원환경지질
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• 제39권6호
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• pp.653-662
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• 2006

화원면 주광리 일대의 석영맥은 선캠브리아기의 변성퇴적암류 내에 발달된 NW 또는 NE방향의 단층대를 충진한 천열수성 석영맥이다. 이들 석영맥의 연장성과 품위를 기초로, 1호 석영맥은 연장성이 200 m이며 폭은 0.1에서 3 m 정도이다. 1호 석영맥의 광화작용은 심성시기와 천성시기로 구분된다. 심성시기는 일라이트, 견운모를 수반하는 필릭대와 점토대로 구성되며 황화광물은 황철석, 유비철석, 섬아연석, 황동석, 방연석 및 함은사면동석 등이 관찰된다 천성시기는 철-망간 산화광물, 아연-철 산화광물 및 연 산화광물 등이 관찰된다. 유체포유물 자료에 의하면, 심성시기의 균일화온도와 염농도는 각각 $187{\sim}306^{\circ}C,\;0.0{\sim}6.2wt.%$로써 광화유체가 계속적인 순환수의 유입에 의한 혼입에 의해 냉각 및 희석작용이 있었음을 시사한다. 산소($-4.1{\sim}4.1%o$), 수소($-107{\sim}-88%o$)동위원소 값을 볼 때 광화유체는 순환수 기윈의 유체가 주종을 이룬 것으로 보이며 광화작용이 진행됨에 따라 계속적인 순환수의 혼입이 크게 작용한 것으로 해석할 수 있다.

• Applied Biological Chemistry
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• 제45권4호
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• pp.185-189
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• 2002

우리나라의 유전자변형농산물 의무 표시제가 시행됨에 따라 수입 유전자변형농산물 중 유전자변형 콩의 혼입유무를 판별할 수 있는 검정기술 개발이 요구되고 있다. 근사미(glyphosate)제초제에 저항성을 나타내는 토양미생물인 Agrobacterium CP4 유래의 5-enolpyruvyl shikimate-3-phosphate synthase(EPSPS) 유전자의 도입여부를 PCR로 진단할 수 있는 특이프라이머를 제작하여 제초제저항성 콩(Roundup Ready Soybean, RRS)을 검정할 수 있는 PCR조건을 확립하였으며 콩의 내재유전자인 lectin유전자와 RRS특이 프라이머를 이용하여 duplex PCR에 의한 제초제저항성 콩의 검정법을 확립하였다. 또한 수입 콩 및 콩나물에 대하여 근사미 제초제 처리로 저항성 개체를 판별하는 생물검정법도 확립하여 저항성 개체의 잎에서 분리한 genomic DNA에 대하여 EPSPS특이 프라이머를 이용하여 분석한 결과 RRS특이적인 PCR밴드를 확인하였다. 또한 수입 콩의 백립중과 종실의 제색을 고려할 때 단일품종이 아닌 여러 품종이 혼합되어 있음을 확인하였다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.27-27
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• 2003

A diversified and concentrative approach of methylation player can be one of the most powerful studies in the understanding of global epigenetic modifications. Previous studies have suggested that DNA methylation contributes to transcriptional silencing through the several DNA methylation-mediated repression systems by hypermethylation, including methyltransferases (DNMTs), DNA methyltransferase association protein 1 (DMAPl), methyl-CpG binding domain (MBD), and histone deacetylases (HDACs). Assembly of these regulatory protein complexes act sequentially, reciprocally, and interdependently on the newly composed DNA strand through S phase. Therefore, these protein complexes have a role in coupling DNA replication to the designed turn-off system in genome. In this study, we attempted to address the role of DNA methylation by the functional analysis of the methyltransferase molecule, we described the involvement of DMAP1 and DNMTs in cell divistion and the effect of their loss. We also described distinct patterns that DMAP1 and DNMTs are spatially reorganized and displaced from condensing chromosomes as cells progress through mitosis in HeLa cell, COS7, and HIH3T3 cell cycle progressions. DNMT1, DNMT3b, and DMAP1 do not stably contact the genetic material during chromosome compaction and repressive expression. These finding show that the loss of activities of DNMTs and DMAP1 occure stage specifically during the cell cycle, may contribute to the integral balance of global DNA methylation. This is consistent with previous studies resulted in decreased histone acetyltransferases and HDACs, and differs from studies resulted in increased histone methyltransferases. Our results suggest that DNA methylation by DNMTs and DMAP1 during mitosis acts to antagonize hypermethylation by which this mark is epigenetical mitotic-specific methylation.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.349-353
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• 2011

The objective of this study was to investigate the relationship between estrous expression, body condition score (BCS), blood urea nitrogen (BUN) and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Sixty, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2 ${\alpha}$ was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100 ${\mu}g$ GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. The estrous inducement rate and estrous expression rate were significantly lower for cows with BCS below 2.25 than for cows with BCS above 2.25. There was 50.0% of rate of mounting in cows with BCS below 2.25 whereas the rate of mounting was markedly increased in cows with BCS above 2.25 (94.1% and 89.5% for BCS 2.25~2.75 and BCS above 2.75 cows, respectively). Cows with BCS <2.25, 2.25~2.75 and ${\geq}$2.75 had number of transferable embryos of $4.5{\pm}0.7$, $5.9{\pm}1.8$ and $5.6{\pm}2.3$ respectively.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.335-339
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• 2011

The objective of this study was to investigate the relationship between concentration of urea nitrogen, glucose, cholesterol and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Fifty five, at random stages of the estrous cycle, received a CIDR. Four days later the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg $PGF_2$ ${\alpha}$ was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100 ${\mu}g$ GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. Cows with BUN < 10, 11~18 and ${\geq}$19 mg/dl had number of transferable embryos of $4.32{\pm}1.3$, $5.8{\pm}1.8$ and $4.7{\pm}2.1$ respectively. The mean numbers of total ova from < 10 and 10${\leq}$ of corpora lutea(CL) was 8.9 and 14.3, respectively. The number of transferable embryos differed between < 10${\leq}$ CL was 4.8 and 5.6, respectively.

• 한국산학기술학회논문지
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• 제21권7호
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• pp.312-320
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• 2020

본 연구의 목적은 Ribosomal Database Project에서 공적으로 활용 가능한 16S rRNA 유전자 시퀀스들의 메타분석을 통해 반추위 고세균의 계통발생 다양성을 조사하는 것이다. 총 8,416개의 시퀀스가 Ribosomal Database Project(출시버전 11, 업데이트 5)로부터 회수되었고, taxonomy tree를 구축하는데 사용되었다. Species 수준의 OTUs가 97% sequence 유사성 기준으로 QIIME 프로그램을 사용하여 분석되었다. 총 8,416개의 시퀀스 중에서 8,412개의 시퀀스는 총 3개의 문으로 분류되었고, 나머지 4개의 시퀀스는 어떤 알려진 문으로 분류되지 못했다. Euryarchaeota는 가장 우점하는 문으로, 전체 고세균 시퀀스의 99.8%를 차지하였다. 이 중에서 차례로 Methanobrevibacter가 65.4%, Methanosphaera가 10.4%, Methanomassillicoccus가 10.4%, Methanomicrobium가 7.9%, Methanobacterium가 1.9%, Methanimicrococcus가 0.5%, Methanosarcina가 0.1%, Methanoculleus가 0.1%를 차지하였다. V2와 V3 영역으로 자른 7,544개의 시퀀스는 493개의 OTUs로 분류되었다. 총 493 OTUs 중에서 단지 17개만 우점하였고, 총 7,544 시퀀스 중 1% 이상을 차지하였다. 본 연구는 반추동물로 부터 메탄발생에 크게 기여하는 반추위 우점 메탄생성균 분석에 대한 향후 연구를 인도하는데 도움을 주고, 사료나 가축품종이 달라져도 이러한 메탄생성균을 억제하는 메탄저감제를 개발하는데 도움을 줄 것이다.

• Reproductive and Developmental Biology
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• 제32권2호
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• pp.117-121
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• 2008

Our previous study showed that transgenic (TG) pigs harboring human EPO (hEPO) gene have been shown to have reproductive disorders, including low pregnancy rates, irregular estrus cycle and low little size. To investigate these reasons, we assessed estrus behavior (standing response) and plasma $17{\beta}$-estradiol ($E_2$) level, which partly reflect reproductive function, during the estrus cycles after synchronization and superovulation by hormone treatments. Then, we analysed blood composition and expression of hEPO gene in TG pigs. Pigs were injected with PG600. After 10 days, pigs were fed with Regumate porcine for 6 days. Blood samples were collected from jugular vein. Analysis of blood composition and $E_2$ level were measured by Hemavet 950 and $E_2$ ELISA kit, respectively. And, the expression of hEPO gene in reproductive organs was quantitated by real-time RT-PCR. The percentage of estrus behavior in TG was significantly decreased. Hematocrit (HCT), hemoglobin (Hb) concentration and red blood cell (RBC) number were significantly higher in TG than wild type (WT). On the other hand, high expression of hEPO gene in TG was observed in the mammary gland as well as in the uterus. Moreover, plasma $E_2$ level was significantly higher in TG than WT. These results suggest that nonspecific expression of hEPO gene in the other organs of TG may affect blood composition and plasma $E_2$ level, thereby causing reproductive disorders.

• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.294-304
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• 2020

Objective: The study was conducted to evaluate the effects of the absorbent (a mixture of activated carbon and hydrated sodium calcium aluminosilicate) on growth performance, blood profiles and hepatic genes expression in broilers fed diets naturally contaminated with aflatoxin. Methods: A total of 1,200 one-day-old male chicks were randomly assigned to 6 treatments with 10 replicate cages per treatment. The dietary treatments were as follows: i) control (basal diets); ii) 50% contaminated corn; iii) 100% contaminated corn; iv) control+1% adsorbent; v) 50% contaminated corn+1% absorbent; vi) 100% contaminated corn+1% absorbent. Results: During d 1 to 21, feeding contaminated diets reduced (p<0.05) body weight (BW), average daily gain (ADG), and average daily feed intake (ADFI), but increased (p<0.05) feed-to-gain ratio (F/G). The absorbent supplementation increased (p<0.05) BW, ADG, and ADFI. There were interactions (p<0.05) in BW, ADG, and ADFI between contaminated corn and absorbent. Overall, birds fed 100% contaminated diets had lower (p<0.05) final BW and ADG, but higher (p<0.05) F/G compared to those fed control diets. The absorbent addition increased (p<0.05) serum albumin concentration on d 14 and 28 and total protein (TP) level on d 28, decreased (p<0.05) alanine transaminase activity on d 14 and activities of aspartate aminotransferase and alkaline phosphatase on d 28. Feeding contaminated diets reduced (p<0.05) hepatic TP content on d 28 and 42. The contaminated diets upregulated (p<0.05) expression of interleukin-6, catalase (CAT), and superoxide dismutase (SOD), but downregulated (p<0.05) glutathione S-transferase (GST) expression in liver. The absorbent supplementation increased (p<0.05) interleukin-1β, CAT, SOD, cytochrome P450 1A1 and GST expression in liver. There were interactions (p<0.05) in the expression of hepatic CAT, SOD, and GST between contaminated corn and absorbent. Conclusion: The results suggest that the naturally aflatoxin-contaminated corn depressed growth performance, while the adsorbent could partially attenuate the adverse effects of aflatoxin on growth performance, blood profiles and hepatic genes expression in broilers.