• 한국작물학회지
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• 제57권4호
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• pp.365-372
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• 2012

Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.1912-1921
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• 2020

Objective: This study assessed genomic prediction accuracies based on different selection methods, evaluation procedures, training population (TP) sizes, heritability (h²) levels, marker densities and pedigree error (PE) rates in a simulated Korean beef cattle population. Methods: A simulation was performed using two different selection methods, phenotypic and estimated breeding value (EBV), with an h² of 0.1, 0.3, or 0.5 and marker densities of 10, 50, or 777K. A total of 275 males and 2,475 females were randomly selected from the last generation to simulate ten recent generations. The simulation of the PE dataset was modified using only the EBV method of selection with a marker density of 50K and a heritability of 0.3. The proportions of errors substituted were 10%, 20%, 30%, and 40%, respectively. Genetic evaluations were performed using genomic best linear unbiased prediction (GBLUP) and single-step GBLUP (ssGBLUP) with different weighted values. The accuracies of the predictions were determined. Results: Compared with phenotypic selection, the results revealed that the prediction accuracies obtained using GBLUP and ssGBLUP increased across heritability levels and TP sizes during EBV selection. However, an increase in the marker density did not yield higher accuracy in either method except when the h² was 0.3 under the EBV selection method. Based on EBV selection with a heritability of 0.1 and a marker density of 10K, GBLUP and ssGBLUP_0.95 prediction accuracy was higher than that obtained by phenotypic selection. The prediction accuracies from ssGBLUP_0.95 outperformed those from the GBLUP method across all scenarios. When errors were introduced into the pedigree dataset, the prediction accuracies were only minimally influenced across all scenarios. Conclusion: Our study suggests that the use of ssGBLUP_0.95, EBV selection, and low marker density could help improve genetic gains in beef cattle.

• Journal of Animal Science and Technology
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• 제55권2호
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• pp.81-85
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• 2013

This experiment was conducted to investigate the effects of lactation records in Holstein dairy cattle on the selection rates using a total of 341,436 test records from 113, 812 heads of cattle from first to third lactation. Heritabilities for milk, fat, and protein yields were highest at first lactation (0.28, 0.24, and 0.27, respectively), and decreased to 0.14, 0.15, and 0.13 at third lactation. For the milk yields, phenotypic correlations between first and second lactation, first and third lactation, and second and third lactation were low (0.49, 0.39, and 0.47, respectively), while genetic correlations among consecutive lactations and between second and third were above 0.8 and 0.9. In Model I, of the 1,138 heads the top 1% were selected based on first lactation records, only 32.4% (396 heads) were re-selected when the second lactation records were included and the 67.6% (769 heads) were newly selected animals. While in Model II, 85.1% (1,138 heads) of the animals which were selected as the top 1% on the basis of first and second lactation records were included. A multiple trait evaluation method using multiple lactation records is more desirable than a single trait evaluation method using first lactation records only.

• Parasites, Hosts and Diseases
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• 제50권3호
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• pp.233-238
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• 2012

The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. The aim of this study was production and evaluation of the usefulness of the recombinant Toxoplasma gondii GRA7 antigen for the serodiagnosis of Toxoplasma gondii IgM and IgG by ELISA. A total of 70 T. gondii IgM positive sera, 74 T. gondii IgG positive sera, and 60 sera from subjects who were not infected with T. gondii were examined. These sera were shown different absorbance values in ELISA test. To control the specificity of the rGRA7 other parasitic diseases, for example, echinococcosis, malaria, leishmaniasis, fascioliasis, and strongyloidiasis were tested of which none showed positive results. Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (com ELISA) were 89% and 90%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 96% and 90%, respectively. The results obtained here show that this antigen is useful for diagnostic purposes.

• Ecology and Resilient Infrastructure
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• 제2권1호
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• pp.33-41
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• 2015

본 연구는 어류서식적합도 향상을 위해 유전자알고리즘을 이용한 하천의 구조개선 방안을 제시하였다. 하천구조 개선에 의한 수리특성의 변화에 따른 어류 서식적합도와 물리적 교란양상과의 상관특성을 분석하였다. 원주천의 하천 환경조사와 어류의 군집특성을 이용하여 수중 생태계를 대표할 수 있는 복원 목표어종으로 참갈겨니를 선택하였다. 참갈겨니의 서식적합지수를 사용한 서식적합도 분석은 PHABSIM 모형을 이용하였다. HEC-RAS를 이용한 수리특성 분석과 하천교란 평가방법을 이용하여 물리적 교란평가를 수행하였다. 서식적합도 향상을 위한 최적의 저수로 폭의 개선방안을 제시하였다. 하천구조의 개선에 의한 수리특성의 변화에 따른 서식적합도와 물리적 교란의 상관성을 분석하여 어류 서식적합도와 물리적 교란 평가점수의 향상이 있음을 확인하였다. 서식적합도 향상을 위한 하천의 구조 개선은 물리적 교란의 평가점수의 향상을 가져옴을 확인하였다.

• 한국지진공학회논문집
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• 제9권2호통권42호
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• pp.17-27
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• 2005

점탄성감쇠기가 설치된 구조물의 통합최적설계기법 및 비용효율성 평가기법을 제시하였다. 구조부재와 점탄성감쇠기의 사용량을 설계변수로 하여, 생애주기비용을 최소화하도록 최적화문제를 정식화하였으며, 유전자알고리즘을 적용하여 최적의 설계변수를 검색하였다. 수치예제에 대한 통합최적설계 수행 결과를 통해 지반운동 특성에 따른 점탄성감쇠기의 최적배치 및 각 층 강성의 최적분포 경향을 분석하였으며, 점탄성감쇠기가 설치되지 않은 구조시스템과의 생애주기비용 비교를 통하여 비용효율성을 평가하였다. 점탄성감쇠기는 특히 중약진지역에서 높은 비용효율성을 보이는 것으로 나타났다.

• Journal of Ginseng Research
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• 제41권3호
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• pp.326-329
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• 2017

Background: Cultivated ginseng is often introduced as a substitute and adulterant of Russian wild ginseng due to its lower cost or misidentification caused by similarity in appearance with wild ginseng. The aim of this study is to develop a simple and reliable method to differentiate Russian wild ginseng from cultivated ginseng. Methods: The mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 3 regions of Russian wild ginseng and Chinese cultivated ginseng were analyzed. Based on the multiple sequence alignment result, a specific primer for Russian wild ginseng was designed by introducing additional mismatch and allele-specific polymerase chain reaction (PCR) was performed for identification of wild ginseng. Real-time allele-specific PCR with endpoint analysis was used for validation of the developed Russian wild ginseng single nucleotide polymorphism (SNP) marker. Results: An SNP site specific to Russian wild ginseng was exploited by multiple alignments of mitochondrial nad7 intron 3 regions of different ginseng samples. With the SNP-based specific primer, Russian wild ginseng was successfully discriminated from Chinese and Korean cultivated ginseng samples by allele-specific PCR. The reliability and specificity of the SNP marker was validated by checking 20 individuals of Russian wild ginseng samples with real-time allele-specific PCR assay. Conclusion: An effective DNA method for molecular discrimination of Russian wild ginseng from Chinese and Korean cultivated ginseng was developed. The established real-time allele-specific PCR was simple and reliable, and the present method should be a crucial complement of chemical analysis for authentication of Russian wild ginseng.

• 정보처리학회논문지C
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• 제18C권3호
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• pp.179-186
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• 2011

무선 메쉬 네트워크 기술은 유선과 비슷한 전송속도를 갖는 무선 네트워크망을 구축하는 기술을 의미하며, 기존 유선 네트워크와 비교해서 네트워크 구축에 보다 향상된 유연성을 제공한다. 또한 노드의 이동성이 적고 에너지 영향에도 제약이 적게 따른다는 특징을 갖고 있다. 그러나 다중 경로 설정 및 선택 시에 발생할 수 있는 시스템 오버헤드와 같은 고려되어야 하는 사항들이 많아지게 된다. 따라서 이러한 네트워크 특성과 경로 설정 기술이 반영될 수 있는 네트워크의 설계 및 최적화에 주목하게 된다. 본 논문에서는 다중 경로 설정에 효과적으로 대응하기 위해 유전 알고리즘의 적합성 평가 함수에 링크의 성능과 로드를 고려한 노드의 트래픽 상황과 데이터 손실률 그리고 대역폭 및 링크의 홉수를 이용한 노드의 서비스 평가 값을 적용함으로써 무선 메쉬 네트워크 라우팅의 경로 설정에 대한 해결 방법을 제안한다.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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• pp.679-682
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• 2003

In this paper, we propose a Genetic Algorithm (GA) using symbiotic evolutionary viruses. Our GA is based on both the building block hypothesis and the virus theory of evolution. The proposed GA aims to control a destruction of building blocks by discovering, keeping, and propagating of building blocks based on virus operation. Concretely, we prepare the group of individuals and the group of viruses. In our GA, the group of individuals searches solutions and the group of viruses searches building blocks. These searches done based on the symbiotic relation of both groups. Also, our GA has two types of virus evaluation techniques. One is that each virus is evaluated by the difference of the fitness of an individual between before and after infection of virus. Another is that all viruses aye evaluated by the difference of the fitness of an individual between before and after infection of all viruses. Furthermore, we applied the proposed GA to the minimum value search problem of a test function which has some local solutions far from the optimal solution. And, we discuss a difference of behaviors of the proposed GA based on each virus evaluation techniques.

• Journal of Microbiology and Biotechnology
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• 제30권12호
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• pp.1862-1869
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• 2020

The spread of plasmid-mediated colistin resistance has posed a serious threat to public health owing to its effects on the emergence of pandrug-resistant bacteria. In this study, we investigated the prevalence and characteristics of mcr-1-positive Escherichia coli isolated from retail meat samples in Korea. In total, 1,205 E. coli strains were isolated from 3,234 retail meat samples in Korea. All E. coli strains were subjected to antimicrobial susceptibility testing and were examined for the presence of mcr-1 gene. All mcr-1-positive E. coli (n = 10, 0.8%) from retail meat were subjected to pulse-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS). The transferability of mcr-1 gene was determined by conjugation assays. The mcr-1-positive strains exhibited diverse clonal types. Our mcr-1 genes were located in plasmids belonged to the IncI2 (n = 1) and IncX4 (n = 8) types, which were reported to be prevalent in Asia and worldwide, respectively. Most mcr-1 genes from mcr-1-positive strains (9/10) were transferable to the recipient strain and the transfer frequencies ranged from 2.4 × 10-3 to 9.8 × 10-6. Our data suggest that the specific types of plasmid may play an important role in spreading plasmid-mediated colistin resistance in Korea. Furthermore, our findings suggest that the retail meat may be an important tool for disseminating plasmid-mediated colistin resistance.