Production and Evaluation of Toxoplasma gondii Recombinant GRA7 for Serodiagnosis of Human Infections
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Selseleh, Mina
(Department of Medical Parasitology and Mycology, School of Public Health (Affiliation Center), Tehran University of Medical Sciences)
Keshavarz, Hossein (Department of Medical Parasitology and Mycology, School of Public Health (Affiliation Center), Tehran University of Medical Sciences) Mohebali, Mehdi (Department of Medical Parasitology and Mycology, School of Public Health (Affiliation Center), Tehran University of Medical Sciences) Shojaee, Saeedeh (Department of Medical Parasitology and Mycology, School of Public Health (Affiliation Center), Tehran University of Medical Sciences) Selseleh, Monavar (Department of Medical Parasitology and Mycology, School of Public Health (Affiliation Center), Tehran University of Medical Sciences) Eshragian, Mohammad Reza (Epidemiology and Biostatistics Department, School of Public Health, Tehran University of Medical Sciences) Mansouri, Fatemeh (Genetic Faculty, School of Public Health, Tehran University of Medical Sciences) Modarressi, Mohammad Hossein (Genetic Faculty, School of Public Health, Tehran University of Medical Sciences) |
1 | Tenter AM, Johnson AM. Recognition of recombinant Toxoplasma gondii antigens by human sera in an ELISA. Parasitol Res 1991; 77: 197-203. DOI |
2 | Redlich A, Müller WA. Serodiagnosis of acute toxoplasmosis using a recombinant form of the dense granule antigen GRA6 in an enzyme linked immunosorbent assay. Parasitol Res 1998; 84: 700-706. DOI |
3 | Fuccillo DA, Madden DL, Tzan N, Sever JL. Difficulties associated with serological diagnosis of Toxoplasma gondii infections. Diagn Clin Immunol 1987; 5: 8-13. |
4 | Pietkiewicz H, Hiszczynska-Sawicka E, Kur J, Petersen E, Nielsen HV, Paul M, Stankiewicz M, Myjak P. Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis. Parasitol Res 2007; 100: 333-337. DOI ScienceOn |
5 | Pietkiewicz H, Hiszczynska-Sawicka E, Kur J, Petersen E, Nielsen HV, Stankiewicz M, Andrzejewska I, Myjak P. Usefulness of Toxoplasma gondii specific recombinant antigens in serodiagnosis of human toxoplasmosis. J Clin Microbiol 2004; 42: 1779-1781. DOI ScienceOn |
6 | Cesborn MF. Dense-granule organelles of Toxoplasma gondii: Their role in the host parasite relationship. Parasitol Today 1994; 10: 293-296. DOI ScienceOn |
7 | Neudeck A, Stachelhaus S, Nischik N, Striepen B, Reichmann G, Fischer HG. Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7. Microbes Infect 2002; 4: 581-590. DOI ScienceOn |
8 | Coppens I, Dunn JD, Romano JD, Pypaert M, Zhang H, Boothroyd JC, Joiner KA. Toxoplasma gondii sequesters lysosomes from mammalian hosts in the vacuolar space. Cell 2006; 125: 261-274. DOI ScienceOn |
9 | Jacobs D, Vercammen M, Saman E. Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain. Clin Diagn Lab Immunol. 1999; 6: 24-29. |
10 | Bothwell A, Yancopoulos GD, Alt FW. Methods for cloning and analysis of eukaryotic genes. Boston, USA. Jones and Bartlett Publishers. 1990, p 247-260. |
11 | Feliciello I, Chinali G. A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal Biochem 1993; 212: 394-401. DOI ScienceOn |
12 | Hiszczynska-Sawicka E, Brillowska-Dabrowska A, Dabrowski S, Pietkiewicz H, Myjak P, Kur J. High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Expr Purif 2003; 27: 150-157. DOI ScienceOn |
13 | Del Bono V, Canessa A, Bruzzi P, Fiorelli MA, Terragna A. Significance of specific immunoglobulin M in the chronological diagnosis of 38 cases of toxoplasmic lymphadenopathy. J Clin Microbiol 1989; 27: 2133-2135. |
14 | Aubert D, Maine GT, Villena I, Hunt JC, Howard L, Sheu M, Brojanac S, Chovan LE, Nowlan SF, Pinon JM. Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. J Clin Microbiol 2000; 38: 1144-1150. |
15 | Nigro M, Gutierrez A, Hoffer AM, Clemente M, Kaufer F, Carral L, Martin V, Guarnera EA, Angel SO. Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis. Diagn Microbiol Infect Dis 2003; 47: 609-613. DOI ScienceOn |
16 | Gabra MS, Grossiord D, Perrin LH, Shaw A, Cheung A, McGregor IA. Defined Plasmodium falciparum antigens in malaria serology. Bull World Health Organ 1986; 64: 889-896. |
17 | Velmurugan GV, Tewari AK, Rao JR, Baidya S, Kumar MU, Mishra AK. High-level expression of SAG1 and GRA7 gene of Toxoplasma gondii (Izantnagar isolate) and their application in serodiagnosis of goat toxoplasmosis. Vet Parasitol 2008; 154: 185-192. DOI ScienceOn |
18 | Sadeghiani G, Zare M, Babaie J, Shokrgozar MA, Azadmanesh K, Fard-Esfahani P, Golkar M. Heterologous production of dense granule GRA7 antigen of Toxoplasma gondii in Escherichia coli. Southeast Asian J Trop Med Public Health 2009; 40: 692-700. |
19 | Li S, Galvan G, Araujo FG, Suzuki Y, Remington JS, Parmley S. Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens. Clin Diagn Lab Immunol 2000; 7: 781-787. |
20 | Pfrepper KI, Enders G, Gohl M, Krczal D, Hlobil H, Wassenberg D, Soutschek E. Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin Diagn Lab Immunol 2005; 12: 977-982. |
21 | Fehniger TE, Walfield AM, Cunningham TM, Radolf JD, Miller JN, Lovett MA. Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen. Infect Immun 1984; 46: 598-607. |
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