• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.289-297
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• 2020

The genetic diversity of two subpopulations of Corynandra chelidonii, one of terrestrial and the other of aquatic environments, was measured with molecular markers, such as start codon targeted (SCoT), inter simple sequence repeats (ISSR), and random amplification of polymorphic DNA (RAPD). The traditional morphological traits such as habitat, habit, leaf morphology, the colour of the sepals and petals, number of stamens, and seed morphology formed the base for their realization as two varieties, C. chelidonii var. pallae and C. chelidonii var. chelidonii. The polymorphism between the two variants was 100% with the primers SCoT-2 and OPA-1 and 4, while maximum polymorphism was detected with ISSR-2, SCoT-3, and OPA-3. The study used, for the first time, more than one molecular marker to assess the genetic variation underscoring the morphological variation in Corynandra chelidonii (L.f.) Cochrane & Iltis. The study justifies the recognition of the two subpopulations of Corynandra chelidonii from aquatic and terrestrial environments as two distinct varieties, C. chelidonii var. pallae (Reddy & Raju) V.S.Raju and C. chelidonii var. chelidonii, respectively, based on the traditional taxonomic evidence.

• Journal of Animal Science and Technology
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• v.47 no.3
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• pp.341-354
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• 2005

For the genetic assessment of the cattle breeds including Hanwoo, eleven microsatellite markers on ten bovine autosomes were genetically characterized for 618 individuals of nineteen cattle breeds; North Eastern Asian breeds (Korean cattle, Korean Black cattle, Japanese Black cattle, Japanese Brown cattle, Yanbian cattle), Chinese yellow cattle (Luxi cattle, Nanyang cattle), European Bas taurus (Angus, Hereford, Charolais, Holstein, Limousin), African Bas taurus (N'Dama, Baoule), African Bas indicus (Kavirondo Zebu, White Fulani), Asian Bas indicus (Sahiwal, Nelore) and one Bali cattle, Bas banteng as an outbreed-reference population. Allele frequencies derived from the genotyping data were used in estimating heterozygosities, gene diversities and genetic distances. The microsatellite loci were highly polymorphic, with a total of 162 different alleles observed across all loci. Variability in allele numbers and frequencies was observed among the breeds. The average expected heterozygosity of North Eastern Asian breeds was higher than those of European and African taurines, but lower than those of Asian and African indicines. Genetic distances were estimated using Nei's DA genetic distance and the resultant DA matrix was used in the construction of the phylogenetic trees. The genetic distances between North Eastern Asian cattle breeds and Bas indicus were similar with those between European Bas taurus and Bas indicus, and African Bas taurus and Bas indicus, respectively. The clusters were clearly classified into North Eastern Asian, European and African taurines groups as well as different cluster with Chinese mainland breeds, firstly out-grouping with Bas indicus. These results suggest that Korean cattle, Hanwoo, had not been originated from a crossbred between Bas primigenius in Europe and Bas indicus in India and North Eastern Asian Bas taurus may be have separate domestication from European and African Bas taurus.

• Journal of Environmental Impact Assessment
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• v.22 no.2
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• pp.157-170
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• 2013

수질오염총량관리제 시행 하천에 대해서는 객관적이고 과학적 방법으로 유역내 각 지역의 오염부하량을 할당할 필요가 있다. 본 연구에서는 대도시에서 배출되는 오염부하의 영향을 크게 받는 영산강수계의 중상류부를 대상으로 오염부하량 할당 방법에 대해 검토하였다. 오염부하량 할당을 위한 수질모델링은, 수질관리에 흔히 적용되어온 QUAL2E의 최근 판인, QUAL2Kw를 이용해서 수행하였다. 모델 적용 대상 지역의 각 reach의 수질매개변수는 QUAL2Kw의 자동보정 기능을 이용해서 추정하였다. 오염부하량 할당의 최적화는 유전알고리즘(genetic algorithm)을 이용하였고, 최소부하량 삭감법(least waste load removal allocation), 일정 부하량 이상 최소부하량 삭감법(least waste load removal over a certain value), 동일삭감률 할당법(equal removal rate)의 세가지 방법을 적용하고 비교 검토하였다. 동일삭감률 할당법은 다른 방법보다 유역 전체 부하량 삭감량이 훨씬 크기 때문에 효과적이지 않았고, 이 방법을 쓰기 위해서는 부하량 삭감대상인 각 소유역과 하수처리장을 그 규모와 특성에 따라 세분화할 필요가 있다. 동일삭감률 할당법의 적용시 세가지 범주로 나누어서 삭감률을 적용하였다. 오염부하량 삭감의 효율성을 감안할 때 최소 부하량 삭감법보다 일정 부하량 이상 최소부하량 삭감법이 더 적절한 것으로 검토되었다.

• Mycobiology
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• v.45 no.1
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• pp.9-14
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• 2017

Most known species in the Physalacriaceae are saprotrophs that grow on decaying leaves and wood, and approximately 21 genera in the Physalacriaceae have been reported worldwide. During an ongoing survey of indigenous fungi in Korea, four specimens belonging to the Physalacriaceae were collected on Ulleung Island. These specimens were identified as three species based on morphological characteristics and molecular analysis of rDNA-internal transcribed spacer sequences. Three species in three genera were shown to be new records in Korea: Hymenopellis orientalis, Paraxerula hongoi, and Ponticulomyces orientalis. The latter two are the first records of these genera in Korea. In this study, we provide detailed morphological descriptions of these species and describe their phylogenetic position within the Physalacriaceae.

• Applied Biological Chemistry
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• v.51 no.4
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• pp.251-257
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• 2008

Different types of milk products, such as kwacha golla, mawa, cheese, curd, and chocolate are popular in Bangladesh. However, the microbiological safety of these products is poorly understood. This study was performed to assess the microbiological quality of kwacha golla, a local milk product. Kwacha golla samples were collected from ten different areas of Rajshahi and Kushtia regions, and the quality of the each sample was assessed using various parameters including standard plate count, total coliform, fecal coliform, total fungi, and spore-forming bacteria, as well as food-borne microorganisms. Out of 300 samples, total coliform was detected at 56.66% (n= 300), exceeding the minimum allowable limit of 36.66%. Similarly, experiments were carried out with fungi and food-borne pathogens including Escherichia coli, Listeria monocytogenes, Salmonella sp., and Staphylococcus aureus. Results revealed 85.33, 53, and 49.33% of the samples were contaminated by fungi, E. coli, and L. monocytogenes, respectively. However, all samples showed no contaminations of Salmonella sp. and Staphylococcus sp. Therefore, this study could be helpful to the people of Bangladesh by providing information on the possibility of a major health problem caused by the consumption of kwacha golla.

• Journal of Environmental Impact Assessment
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• v.20 no.5
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• pp.663-673
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• 2011

In the face of accelerating biodiversity loss and its significance in our coexistence with nature, biodiversity is becoming more crucial in sustainable development perspective. To estimate biodiversity in the future which provides valuable information for decision making system especially in the national level, a quantitative approach must be studied forehand as a baseline of the present status. In this study, we developed a large-scale map of Plant Species Richness (PSR, typical indicator of biodiversity) for Young-dong and Pyung-chang provinces. Due to the accessibility of appropriate data and advance of modelling techniques, reduction of variables without deteriorating the predictive power is considered by applying Genetic algorithm. In addition, a number of Correctly Classified Instances (CCI) with 10-fold cross validation which indicates the predictive power, was carried out for evaluation. This study, as a fundamental baseline, will be beneficial in future land work as well as ecosystem restoration business or other relevant decision making agenda.

• Journal of Environmental Impact Assessment
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• v.20 no.3
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• pp.357-365
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• 2011

The QUAL2K has the same basic characteristics as the QUAL2E model, which has been widely used in stream water quality modeling; in QUAL2K, however, various functions are supplemented. The QUAL2Kw model uses a genetic algorithm(GA) for automatic calibration of QUAL2K, and it can search for optimum water quality parameters efficiently using the calculation results of the model. The QUAL2Kw model was applied to the Gangneung Namdaecheon River on the east side of the Korean Peninsula. Because of the effluents from the urban area, the middle and lower parts of the river are more polluted than the upper parts. Moreover, the hydraulic characteristics differ between the lower and upper parts of rivers. Thus, the river reaches were divided into seven parts, auto-calibration for the multiple reaches was performed using the function of the user-defined automatic calibration of the rates worksheets. Because GA parameters affect the optimal solution of the model, the impact of the GA parameters used in QUAL2Kw on the fitness of the model was analyzed. Sensitivity analysis of various factors, such as population size, crossover probability, crossover mode, strategy for mutation and elitism, mutation rate, and reproduction plan, were performed. Using the results of this sensitivity analysis, the optimum GA parameters were selected to achieve the best fitness value.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.273-281
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• 2011

Fusarium head blight (FHB), also known as scab, caused mainly by Fusarium graminearum is a devastating disease of wheat in regions that are warm and humid during flowering. In addition to significant yield and quality losses, the mycotoxin deoxynivalenol produced by the pathogen in infected wheat kernels is a serious problem for food and feed safety. Twenty- three Korean cultivars and "Sumai 3", which is a FHB-resistant Chinese cultivar were tested for Type I, Type II resistances of FHB. Three cultivars were identified as resistant in Type I assessment, and two cultivars were resistant in Type II assessment. Genetic variation and relationship among the cultivars were evaluated on the basis of 11 Simple Sequence Repeat (SSR) and 29 Sequence Tagged Site (STS) markers that were linked to FHB resistance Quantitative Trait Loci (QTL) on chromosome 3BS. One SSR and 7 STS markers detected polymorphisms. Especially, using a STS marker (XSTS3B-57), 32.4% of the variation for Type II FHB resistance could be explained. Genetic relationship among Korean wheat cultivars was generally consistent with their released year. These markers on chromosome 3BS have the potential for accelerating the development of Korean wheat cultivars with improved Fusarium head blight resistance through the use of marker-assisted selection.

• Natural Product Sciences
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• v.24 no.4
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• pp.219-224
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• 2018

During the screening for cytotoxic compounds from plants grown in Korea, Betula platyphylla (BP) showed potent activity against the adenocarcinomic human alveolar basal epithelial A549 cell line. To identify the cytotoxic components from BP, the $CH_2Cl_2$ fraction with the most significant cytotoxic effect was applied to the column chromatographies. Seven compounds were isolated: lupeol (1), betulinic acid (2), (-)-rhododendrol (3), platyphyllenone (4), platyphyllone (5), (-)-centrolobol (6), and oleanolic acid (7). Among them, three diarylheptanoids (4 - 6) exhibited cytotoxicity toward A549 cells. Especially, $50{\mu}M$ of 4 reduced A549 cell viability to $18.93{\pm}0.82%$ compared to control ($100.00{\pm}21.48%$). Lactate dehydrogenase (LDH) leakage and intracellular reactive oxygen species (ROS) production were also induced by $50{\mu}M$ 4. This is the first report on the cytotoxic effect of BP-derived diarylheptanoids 4-6 against A549 cells. The compound 4 may be useful for the development of early hit compounds for non-small cell lung carcinoma, but the consideration about selectivity of 4 is required since 4 also showed the cytotoxicity in the human normal lung epithelial BEAS-2B cell line.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.909-912
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• 2006

This paper examined the applicability of convex hull, which is defined as the minimal convex polygon including all points, to assessment model for effective region. In order to validate the applicability of the convex hull to assessment model for effective region, a genetic algorithm was adopted as a optimum technique, and an artificial neural network was adopted as a prediction model for material properties. The mix-proportion obtained from the proposed technique is more reasonable than that obtained from previous work.