• Journal of Pharmaceutical Investigation
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• 제32권3호
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• pp.153-159
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• 2002

Gene therapy is fundamentally a sophisticated drug delivery technology to cure a disease by the transfer of genetic material to modify living cells. In other words, the gene is used as a therapeutic drug much like a chemical compound is employed in chemotherapy. Currently almost 600 clinical trials are underway worldwide since the first clinical trials carried out in 1990 to treat adenosine deaminase deficiency using retroviral vectors. Despite the great progress still is there no gene therapy product being approved as a new drug. This is partly due to a lack of an ideal gene delivery system that is safe and can provide stable, optimal level production of the therapeutic proteins in the cell. This review covers the current status of several different biological and physico-chemical agents that are being developed as gene delivery vehicles. Although gene therapy promises great hopes toward the cure of a broad spectrum of genetic and acquired diseases, the success of gene therapy heavily asks for the development of vector systems for safe and efficient application in humans.

• 한국항해항만학회지
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• 제39권1호
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• pp.61-67
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• 2015

국내의 화물 운송량은 꾸준히 증가하고 있으며 이에 따라 내륙지역의 화물운송량 예측에 관한 연구가 활발하게 진행되고 있다. 하지만 종래의 기종점 자료(O/D Data)는 화물 운송수단의 출발지와 목적지를 조사하여 구축하였으며 이는 화물의 운송 특성을 제대로 반영시키지 못하는 문제점을 지니고 있다. 화물의 운송은 단순히 출발지에서 목적지로 화물을 운반하는 것이 아니라 화물운송 도중에 운송수단을 변경하거나 화물의 임시 보관이 필요한 경우가 발생하므로 화물운송모형에서는 중간 경유지(물류센터 또는 내륙컨테이너기지)를 포함시켜야 된다. 본 논문에서는 이러한 중간 경유지를 고려한 기종점 자료의 필요성을 인식하였고 물류센터를 고려한 화물운송 경로에 대한 모형을 제시하고자 한다. 본 연구가 제시하는 화물운송 경로모형은 각 구간별 운송계수를 설정하고 이에 따라 화물량을 예측하는 모형으로서 유전 알고리즘을 이용하여 모형을 검증하였고 오차의 범위가 ${\pm}1.0%$이내로써 모형은 타당한 것으로 판단된다.

• Journal of Animal Science and Technology
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• 제56권5호
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• pp.17.1-17.4
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• 2014

Puberty is a stage of sexual development determined by the interaction of many loci and environmental factors. Identification of genes contributing to genetic variation in this character can assist with selection for early pubertal bulls, improving genetic progress in livestock breeding. Thyroid hormones play an important role in sexual development and spermatogenic function. The objective of this study was to evaluate the association between single nucleotide polymorphisms (SNPs) located in thyroglobulin(TG) gene with age of puberty in Angus bulls. Four SNPs were genotyped in 273 animals using SEQUENOM technology and the association between markers and puberty age was analyzed. Results showed a significant association (P < 0.05) between these markers and puberty age estimated at a sperm concentration of 50 million and a progressive motility of 10%. This is the first report of an association of TG polymorphisms with age of puberty in bulls, and results suggest the importance of thyroidal regulation in bovine sexual development and arrival to puberty.

• 산업공학
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• 제16권spc호
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• pp.99-104
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• 2003

While demands for express service are rapidly increasing according to recent progress of electronic commerce, express service companies are struggling to take a larger delivery service market share through ongoing improvement in their service processes. Extension of cut-off time for express service centers can provide the express company with increase of total sales, but it may also cause to increase the possibility not to satisfy customer needs due to work delay in the consolidation terminal. Therefore, compromised decision for cut-off time of each service center should be made by taking operation characteristics of the consolidation terminal into account. This study suggests an approach for determining the cut-off time for express service centers according to operational characteristics of the consolidation terminal with the objective of maximizing expected incremental sales. The problem defined in this study can be represented as two successive models; one is an integer programing model in which the best cut-off time for each sales center are determined, and the other is a single machine scheduling model in which a working schedule in the consolidation terminal is obtained. A genetic algorithm is developed to solve the two models simultaneously. Finally, an example problem is carried out to verify applicability and performance of the algorithm with the data set collected from an express company.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2003년도 제45차 추계학술대회
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• pp.21-30
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• 2003

As a result of the technological advance provided by intracytoplasmic sperm injection (ICSI) in 1992, the evaluation and treatment of the infertile male has changed significantly. Many men who were previously thought to be irreversibly infertile have the potential to initiate their own biologic pregnancy. However, not all men having impaired semen parameter are ideal candidates for ICSI for numerous reasons including a lack of addressing the underlying problem causing the male infertility, unknown genetic consequences, and cost-effectiveness issues. In this era of ICSI, the fundamental approach to the male with suspected subfertility is unchanged and is based on a history, physical examination, and focused laboratory testing. The urologist should approach the patient with an intent to identify remediable causes of subfertility given the specific clinical situation. For instance, should a gentleman have his varicocele repaired or vasectomy reversed, or should he proceed directly with ICSI? If no factors can be improved in a timely manner, then ICSI should be considered using the available sperm. Examples of recent advances include the diagnosis and treatment of ejaculatory duct obstruction, indications and techniques for performing testis biopsy, and technique for sperm harvesting. In addition, potential genetic causes of male subfertility should be diagnosed and discussed with the patient. Cystic fibrosis gene mutation, karyotype abnormallities, and Y-chromosome microdeletions all have recently been identified as causative for male infertility in otherwise phenotypically normal men. With recently evolved diagnostic and therapeutic techniques now available for the infertile couple, even the most severe male factor problems in patients previously considered irreversibly infertile are now potentially treatable. The physician should be aware of the availability and limitations of these new and exciting reproductive technologies because they will allow him to provide timely and more effective therapy for the infertile couple. An understanding of these advances by all physicians is important as we progress into the $21^{st}$ century

• 전기학회논문지
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• 제65권7호
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• pp.1236-1241
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• 2016

Cancer has been the most frequent in Korea, and pathogenesis and progression of cancer have been known to be occurred through various causes and stages. Recently, the research of chromosomal and genetic disorder and the research about prognostic factor to predict occurrence, recurrence and progress of chromosomal and genetic disorder have been performed actively. In this paper, we analyzed DNA methylation data downloaded from TCGA (The Cancer Genome Atlas), open database, to research bladder cancer which is the most frequent among urinary system cancers. Using three level of methylation data which had the most preprocessing, 59 candidate CpG island were extracted from 480,000 CpG island, and then we analyzed extracted CpG island applying data mining technique. As a result, cg12840719 CpG island were analyzed significant, and in Cox's regression we can find the CpG island with high relative risk in comparison with other CpG island. Shown in the result of classification analysis, the CpG island which have high correlation with bladder cancer are cg03146993, cg07323648, cg12840719, cg14676825 and classification accuracy is about 76%. Also we found out that positive predictive value, the probability which predicts cancer in case of cancer was 72.4%. Through the verification of candidate CpG island from the result, we can utilize this method for diagnosing and treating cancer.

• Journal of Korean Neurosurgical Society
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• 제65권5호
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• pp.697-709
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• 2022

Objective : The present study aimed to identify the function of ischemic stroke (IS) patients' peripheral blood and its role in IS, explore the pathogenesis, and provide direction for clinical research progress by comprehensive bioinformatics analysis. Methods : Two datasets, including GSE58294 and GSE22255, were downloaded from Gene Expression Omnibus database. GEO2R was utilized to obtain differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs were performed using the database annotation, visualization and integrated discovery database. The protein-protein interaction (PPI) network of DEGs was constructed by search tool of searching interactive gene and visualized by Cytoscape software, and then the Hub gene was identified by degree analysis. The microRNA (miRNA) and miRNA target genes closely related to the onset of stroke were obtained through the miRNA gene regulatory network. Results : In total, 36 DEGs, containing 27 up-regulated and nine down-regulated DEGs, were identified. GO functional analysis showed that these DEGs were involved in regulation of apoptotic process, cytoplasm, protein binding and other biological processes. KEGG enrichment analysis showed that these DEGs mediated signaling pathways, including human T-cell lymphotropic virus (HTLV)-I infection and microRNAs in cancer. The results of PPI network and cytohubba showed that there was a relationship between DEGs, and five hub genes related to stroke were obtained : SOCS3, KRAS, PTGS2, EGR1, and DUSP1. Combined with the visualization of DEG-miRNAs, hsa-mir-16-5p, hsa-mir-181a-5p and hsa-mir-124-3p were predicted to be the key miRNAs in stroke, and three miRNAs were related to hub gene. Conclusion : Thirty-six DEGs, five Hub genes, and three miRNA were obtained from bioinformatics analysis of IS microarray data, which might provide potential targets for diagnosis and treatment of IS.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권5호
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• pp.237-251
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• 2002

The recent progress on metabolic systems engineering was reviewed based on our recent research results in terms of (1) metabolic signal flow diagram approach, (2) metabolic flux analysis (MFA) in particular with intracellular isotopomer distribution using NMR and/or GC-MS, (3) synthesis and optimization of metabolic flux distribution (MFD), (4) modification of MFD by gene manipulation and by controlling culture environment, (5) metabolic control analysis (MCA), (6) design of metabolic regulation structure, and (7) identification of unknown pathways with isotope tracing by NMR. The main characteristics of metabolic engineering is to treat metabolism as a network or entirety instead of individual reactions. The applications were made for poly-3-hydroxybutyrate (PHB) production using Ralstonia eutropha and recombinant Escherichia coli, lactate production by recombinant Saccharomyces cerevisiae, pyruvate production by vitamin auxotrophic yeast Toluropsis glabrata, lysine production using Corynebacterium glutamicum, and energetic analysis of photosynthesic microorganisms such as Cyanobateria. The characteristics of each approach were reviewed with their applications. The approach based on isotope labeling experiments gives reliable and quantitative results for metabolic flux analysis. It should be recognized that the next stage should be toward the investigation of metabolic flux analysis with gene and protein expressions to uncover the metabolic regulation in relation to genetic modification and/ or the change in the culture condition.

• Molecules and Cells
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• 제38권9호
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• pp.743-749
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• 2015

Production of genome-edited animals using germline-competent cells and genetic modification tools has provided opportunities for investigation of biological mechanisms in various organisms. The recently reported programmed genome editing technology that can induce gene modification at a target locus in an efficient and precise manner facilitates establishment of animal models. In this regard, the demand for genome-edited avian species, which are some of the most suitable model animals due to their unique embryonic development, has also increased. Furthermore, germline chimera production through longterm culture of chicken primordial germ cells (PGCs) has facilitated research on production of genome-edited chickens. Thus, use of avian germline modification is promising for development of novel avian models for research of disease control and various biological mechanisms. Here, we discuss recent progress in genome modification technology in avian species and its applications and future strategies.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.469-473
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• 1992

Data on the birth weight, 8 week body weight and ewe body weight of an unselected random mating Romney Marsh sheep flock are analyzed to study the influence of various environmental factors. The average birth weight of lambs was 5.06 kg. Sex of lamb, birth type and dam age contributed significant variation in lamb birth weight (p < 0.05). Males were significantly heavier than females (p < 0.05) and singles were significantly heavier than multiples (p < 0.05) at birth. Birth weight of lamb increased with the progress of dams' age. The overall average 8 week body weight of lambs was 20.84 kg. Effect of birth weight, sex, birth type and dam age was significant on 8 week body weight of lamb. Eight week body weight increased with the increase of lamb birth weight (b=1.285 kg). Ewes' body weight taken before tupping was affected by ewes' age, year of performance and their weight at birth. It was concluded that performance data on lamb birth weight, 8 week body weight and ewe body weight should be corrected for the above relevantly significant environmental factors in any genetic calculation in the United Kingdom Romney March sheep.