• Clinical and Experimental Reproductive Medicine
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• 제38권3호
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• pp.126-134
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• 2011

Preimplantation genetic diagnosis (PGD) is gradually widely used in prevention of gene diseases and chromosomal abnormalities. Much improvement has been achieved in biopsy technique and molecular diagnosis. Blastocyst biopsy can increase diagnostic accuracy and reduce allele dropout. It is cost-effective and currently plays an important role. Whole genome amplification permits subsequent individual detection of multiple gene loci and screening all 23 pairs of chromosomes. For PGD of chromosomal translocation, fluorescence $in-situ$ hybridization (FISH) is traditionally used, but with technical difficulty. Array comparative genomic hybridization (CGH) can detect translocation and 23 pairs of chromosomes that may replace FISH. Single nucleotide polymorphisms array with haplotyping can further distinguish between normal chromosomes and balanced translocation. PGD may shorten time to conceive and reduce miscarriage for patients with chromosomal translocation. PGD has a potential value for mitochondrial diseases. Preimplantation genetic haplotyping has been applied for unknown mutation sites of single gene disease. Preimplantation genetic screening (PGS) using limited FISH probes in the cleavage-stage embryo did not increase live birth rates for patients with advanced maternal age, unexplained recurrent abortions, and repeated implantation failure. Polar body and blastocyst biopsy may circumvent the problem of mosaicism. PGS using blastocyst biopsy and array CGH is encouraging and merit further studies. Cryopreservation of biopsied blastocysts instead of fresh transfer permits sufficient time for transportation and genetic analysis. Cryopreservation of embryos may avoid ovarian hyperstimulation syndrome and possible suboptimal endometrium.

• 한국초지조사료학회지
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• 제39권3호
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• pp.189-194
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• 2019

The normal feeding approach of goats might be due to their precise anatomical and physiological characteristics of entity, which permit them to be highly selective, to eat legume silages and wild green grass. This review has been designed to consider the grazing behavior, fodder selection, and feed composition of goats. Various herbs and corns consumed by goats have numerous nutritive resources. Based on the general herbaceous intake activities and behavior of goats, they prefer wild grass such as grass grown in the steep hills than soft grass. Because the digestion capacity of cellulose feed has higher digestion level compared to other non-ruminants within rumen and it is advantageous to use wild forest or mountain grass which comprises high proportion of cellulose feed for goat. In South Korea, there are abundant feed resources for goats because of occupying large areas of mountains. Thus, goat production and feeding costs could be reduced if plants are used from the wild forest as a feed for goats relative to grassland grazing. Also, it is expected to contribute in improvement of goat farming with harmonious relationship between the grassland and wild forest while satisfying animal welfare and physiological desires of livestock.

• Animal Bioscience
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• 제35권8호
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• pp.1151-1161
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• 2022

Objective: This study was conducted to estimate the association of age at first calving (AFC) with first lactation traits as well as lifetime performance traits in Murrah buffaloes. Methods: Data on first lactation and life time performance of Murrah buffaloes (n = 679), maintained at Indian Council of Agricultural Research-Central Institute for Research on Buffaloes, Hisar, India during the period 1983 through 2017, were deduced to calculate heritability estimates, genetic and phenotypic correlation of different first lactation and lifetime traits. The univariate animal model was fitted to estimate variance components and heritability separately for each trait, while bivariate animal models were set to estimate genetic and phenotypic correlations between traits under study. Results: The heritability was high for first peak milk yield (FPY, 0.64±0.08), moderate for AFC (0.48±0.07) and breeding efficiency (BE 0.39±0.09). High genetic correlations of first lactation total milk yield (FLTMY) with first lactation standard milk yield (FLSMY, 305 days or less), FPY, and first lactation length (FLL) was seen. Likewise, genetic correlation of AFC was positive with FLTMY, FLL, first dry period (FDP), first service period (FSP), first calving interval (FCI), herd life (HL) and productive days (PD). Significant phenotypic correlation of FLTMY was observed with HL, productive life (PL), PD, total lifetime milk yield (LTMY), standard lifetime milk yield (standard LTMY). Moreover, positive genetic and phenotypic correlation of FPY was observed with HL, PL, PD, total LTMY and standard LTMY. Conclusion: This study reports that AFC had positive genetic correlation with FDP, FSP, FCI, and unproductive days while, negative association of AFC was observed with FLSMY, PL, total LTMY, standard LTMY, and BE. This suggests that reduction of AFC would results in improvement of lifetime performance traits.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.298-308
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• 2020

Genetic diversity among Thaumatococcus daniellii populations in the southwestern region of Nigeria were assessed using morphometric and molecular markers to determine the population structure and existing genetic relationship for its improvement, conservation and sustainable utilisation. Populations from five locations in each of the six states were used for the study. Morphometric data were collected on folia characters and analysed for variability. Genome DNA was isolated from the plant leaf and amplified by polymerase chain reaction with inter-simple sequence repeat markers (ISSR) to determine the allelic polymorphism, marker effectiveness and genetic relationship of the population. The results showed significant variations in petiole length and leaf dimensions of the populations within and across the states. These morphometric traits are the major parameters that delimit the populations and they correlated significantly at P≤0.05. Analysis of the electrophoregram showed that the ISSR markers are effective for the diversity study. A total of 136 loci were amplified with an average of 7.16 loci per marker, 63.2% of the loci were polymorphic. The Principal Coordinate Analysis revealed that seven factors accounted for 81.6% of the variation and the dendrogram separated the populations into two major groups at a genetic distance of 10 (about 90% similarity) with sub-groups and clusters. Most populations within the state had a high degree of similarity, nonetheless, strong genetic relationship exists among populations from different states. The close relationship between populations across the states suggests a common progenitor, which are likely separated by ecological or geographical isolation mechanisms.

• 한국수정란이식학회지
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• 제24권3호
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• pp.199-205
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• 2009

This study was carried out to investigate the effective genetic resources preservation system using the frozen boar semen. The porcine oocytes were matured for 44 hours in NCSU-23 medium with or without 10% Porcine Follicle Fluid (PFF), 0.5 ${\mu}g/ml$ porcine FSH, 0.5 ${\mu}g/ml$ equine LH, 1.0 ${\mu}g/ml$ 17 $\beta$-estradiol ($E_2$) and 10 ng/ml Epidermal Growth Factor (EGF) under mineral oil at $38.5^{\circ}C$ in humidified atmosphere of 5% $CO_2$ in air. After 44 h of culture, the oocytes were inseminated with frozen-thawed semen and fresh semen prepared with mTBM medium for 6 h. Later, set of 50 presumptive zygotes were transferred into 4-well dish (500 ${\mu}l$) of IVC medium. for embryos freezing, slow-freezing and vitrification methods were used as a cryopreservation. Differences among treatments were analyzed using General Linear Model Procedure by SAS Package (version 6.12) differences were considered significant when p<0.05. Following IVF and IVC, the rates of cleavage and blastocysts formation were significantly higher (p<0.05) in hormone supplemented group than that of hormone-free group (25.7 vs, 12.1). The development rates to cleavage and blastocysts were significantly higher in PZM-5 group than NCSU-23 group (60.3%, 46.6% vs 27.4%, 11.1%). Further improvement was achieved when PZM-5 was supplemented with FBS. Cleavage rates was significantly higher in fresh semen source group than frozen semen (66.7% vs 43.7%). However in blastocysts rates was similar two groups. Post-thaw survival rates of embryos were 1.2% and 2.2% in slow-frezing and vitrification groups, respectively. The results of our study suggest that it is still possible to improve the culture conditions and boar semen cryopreservation for enhance reproductive technology and animal genetic resources conservation.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1388-1393
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• 2015

Genetic parameters of Berkshire pigs for reproduction, carcass and meat quality traits were estimated using the records from a breeding farm in Korea. For reproduction traits, 2,457 records of the total number of piglets born (TNB) and the number of piglets born alive (NBA) from 781 sows and 53 sires were used. For two carcass traits which are carcass weight (CW) and backfat thickness (BF) and for 10 meat quality traits which are pH value after 45 minutes (pH45m), pH value after 24 hours (pH24h), lightness in meat color (LMC), redness in meat color (RMC), yellowness in meat color (YMC), moisture holding capacity (MHC), drip loss (DL), cooking loss (CL), fat content (FC), and shear force value (SH), 1,942 pig records were used to estimate genetic parameters. The genetic parameters for each trait were estimated using VCE program with animal model. Heritability estimates for reproduction traits TNB and NBA were 0.07 and 0.06, respectively, for carcass traits CW and BF were 0.37 and 0.57, respectively and for meat traits pH45m, pH24h, LMC, RMC, YMC, MHC, DL, CL, FC, and SH were 0.48, 0.15, 0.19, 0.36, 0.28, 0.21, 0.33, 0.45, 0.43, and 0.39, respectively. The estimate for genetic correlation coefficient between CW and BF was 0.27. The Genetic correlation between pH24h and meat color traits were in the range of -0.51 to -0.33 and between pH24h and DL and SH were -0.41 and -0.32, respectively. The estimates for genetic correlation coefficients between reproductive and meat quality traits were very low or zero. However, the estimates for genetic correlation coefficients between reproductive traits and drip and cooking loss were in the range of 0.12 to 0.17 and -0.14 to -0.12, respectively. As the estimated heritability of meat quality traits showed medium to high heritability, these traits may be applicable for the genetic improvement by continuous measurement. However, since some of the meat quality traits showed negative genetic correlations with carcass traits, an appropriate breeding scheme is required that carefully considers the complexity of genetic parameters and applicability of data.

• 농업과학연구
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• 제2권2호
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• pp.341-356
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• 1975

본 연구는 육성후대의 3개 동기생육형 합성품종과 서부 oregon의 저온다습한 기후 하에 선발된 7개인자형의 다계교배 파생계통들의 능력을 검정하기 위하여 대비품종으로 다수성품종 Fawn을 공시하여 대전의 충남대학교 공과대학 실험포장에서 수행하였다. 특히 광합성과 관련이 깊은 식물체 및 잎의 주요형질과 아울러 재식후 첫여름의 조사로 생산량을 조사 분석하였던바 주요한 결론을 요약하면 다음과 같다. 1) 파생계통 및 합성품종군과 대비품종 상호간에는 생엽중의 유전적인 차이를 나타내었던바 다계교배 파생계통군의 평균 생엽중이 가장 무거웠고 다음이 합성품종군 이었으며 대비품종 Fawn이 가장 가벼웠다. 한편 다계교배 파생계통군 대에서는 인자형들간에 건엽중의 유전적 차이를 나타내고 있었다. 2) 엽면적 역시 파생계통 군합성품종군 및 대비품종간에 유전적 차이를 보였는데 동기생육형 합성품종군의 평균 엽면적이 대비품종 Fawn에 비해 현저히 넓었으며 다계교배 파생계통군이 가장 넓은 평균 엽면적을 보았다. 3) 합성품종군 파생계통군 및 대비품종간에 특정엽중(단위엽면적당엽중)의 차이는 인정되지 않았는데 앞으로 이특저엽중의 중간변이에 관한 연구가 요망된다. 4) 엽폭은 파생계통군, 합성품종군 및 대비품종간에 현저한 유전적인 차이가 인정되었던바 다계교배 파생계통군의 평균엽폭이 가장 넓었고 다음에 동기새육형 합성품종군 이었으며 대비품종 Fawn이 가장 좁았다. 5) 제1회 예취전 초장은 대비품종 Fawn이 동계생육형 합성품종이나 파생계통에 대해 현저히 길었으며 한편7개인자형의 다계교배 파생계통간에서는 초장의 편차가 크게 나타났으나 초장의 재생력은 품종 파생계통간 혹은 내에서 유의차가 인정되지 않았다. 6) 일차 예취전의 주폭(株幅) 즉 포장피복능력은 동기생육형 합성품종에서 컷고 한편 1차예취후의 주폭은 다계교배파생계통들간에 상당한 차이를 나타내었던바 유전적인 차이가 충분히 인정되었다. 8) 제1차예취시의 청예수량은 합성품종의 후기세대에서 상당한 개선을 보았으며 다계교배 파생계통의 7개 인형들간에는 상당한 유전적인 차이를 보였다. 다계교배 파생계통군의 평균수량은 대비 품종이나 동기생육형 합성품종의 수량보다 현저히 높았던바 이로서 보다 수량성이 높은 품종의 개량이 앞으로 가능할 것이라고 추측된다. 9) 여름동안의 청예수량 및 초장 재생력으로 보면 동기생육형 품종군 및 파생계통들간의 재생력에는 대체로 차이가 없었다. 10) 전체적으로 볼때 특정엽중 및 제1회 예취전초장을 제외한 전형질에서 모두 유의성은 인정되는 것은 아니라 하더라도 다계교배 파생계통군이 가장 우수하였고 다음이 합성품종군이었으며 대비품종 Fawn이 가장 떨어졌다. 그리고 다계교배 파생계통의 7개 인자형 간에는 유전적인 차이가 인정되었다. 11) 통계적인 유의성이 모두 인정되는것은 아니나 모두 형질에서 품종 1002가 1001 및 1000두품종보다 우수 하였다. 12) 여러형질간의 상관연구의 결과는 잘일치되고 있는바 보다나은 품종의 개량을 위한 바람직한 인자형의 선발에 기여 할수 있을 것이다.

• 한국잠사곤충학회지
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• 제37권1호
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• pp.10-15
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• 1995

Genetic characterization of Sri Lankan silkworm bivoltine population has not been at-tempted so far, since its sporadic introduction of bivoltine strains into the island, starting from the 1950's. Genetic structure of Sri Lankan population of mulberry silkworm Bombyx mori was investigated through estimation of general (GCA) and specific combining ability(SCA) and heritability(${h^2}_B$), on the economic quantitative characters from leading 8 inbreds and their 28 F1's in a half diallel cross, in an attempt to utilize the estimates in determination of future breeding methods and to predict the breeding value over the phenotypic value. It was found that the breeding population of the bivoltine silkworm in Sri Lanka has still maintained considerable amounts of additive gene action as well as nonadditive. For some time in the future, both breeding strategies of "selection without inbreeding" and also "inbreeding followed by crossing" should therefore be effective in genetic improvement of economic characters investigated. In addition, superior combiners in general and in specific F1′s were identified for each of 6 economic characters, to be immediately utilized in selection and also in cross breeding programs in Sri Lanka.

• 대한산업공학회지
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• 제39권5호
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• pp.361-366
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• 2013

Prediction of process parameters is very important in parameter design. If predictions are fairly accurate, the quality improvement process will be useful to save time and reduce cost. The concept of dual response approach based on response surface methodology has widely been investigated. Dual response approach may take advantages of optimization modeling for finding optimum setting of input factor by separately modeling mean and variance responses. This study proposes an alternative dual response approach based on machine learning techniques instead of statistical analysis tools. A hybrid neural network-genetic algorithm has been proposed for the purpose of parameter design. A neural network is first constructed to model the relationship between responses and input factors. Mean and variance responses correspond to output nodes while input factors are used for input nodes. Using empirical process data, process parameters can be predicted without performing real experimentations. A genetic algorithm is then applied to find the optimum settings of input factors, where the neural network is used to evaluate the mean and variance response. A drug formulation example from pharmaceutical industry has been studied to demonstrate the procedures and applicability of the proposed approach.

• 한국정보과학회논문지:소프트웨어및응용
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• 제33권4호
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• pp.440-448
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• 2006

자기 조직화 신경망 (SOM: Self-Organizing Map)은 자율 학습 신경망으로 사전 지식이 존재하지 않는 자료에 존재하는 구조적 관계성을 보전하는데 이용된다. 자기 조직화 신경망은 벡터 양자화, 조합 최적화, 패턴 인식과 같은 복잡한 문제 해결을 위한 연구에 많이 이용되어 왔다. 이 논문에서는 좀더 효율적인 유전 알고리즘을 얻기 위한 스키마 변환 도구로서 자기 조직화 신경망을 이용하는 새로운 사용법에 대해서 제안한다. 즉, 각 자식해는 탐색 공간에서 좀더 바람직한 모양을 가지는 동질의 인공 신경망으로 변환된다. 이 변환으로 인해 강한 상위(epistasis)를 가지는 유전자들은 염색체 상에서 서로 인접하게 되는 것이다. 실험 결과는 기존 결과에 비해서 주목할만한 성능 개선이 있음을 보여준다.