• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.603-607
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• 2004

Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out with a battery of 11 random decamer primers to study band frequency (BF), genetic identity index (I) and mean average percentage difference (MAPD) between Bhadawari and Murrah breeds of buffalo. The primers OPA04 and BG15 resolved a band of 460 bp, which was present only in animals of Bhadawari breed. Whereas, the primers OPA14, BG27 and BG28 produced Murrah specific fragments of sizes 730 bp and 1,230 bp, respectively. The estimate of genetic identity index was highest (0.845) with the primer OPA01 and the lowest (0.479) with the primer BG27. The genetic identity index pooled over the primers was 0.596${\pm}$0.037 between these two breeds. The highest MAPD estimate (53.9) between the two breeds was obtained with the primer BG27 and the lowest (14.3) with the primer OPA01. It might be concluded that the genetic identity index between these two breeds calculated on the basis of BF showed moderate level of genetic identity with the primers employed. MAPD calculated on the basis of uncommon bands also demonstrated lower to medium level of genetic difference between Bhadawari and Murrah breeds of buffalo.

• Journal of Ecology and Environment
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• 제29권6호
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• pp.503-509
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• 2006

서남해안에 자생하고 있는 염생식물 4종, 갈대(Phragmites communis Trin), 칠면초(Suaeda japonica Makino), 갯잔디(Zoysia sinica Hance), 그리고 해홍나물(Suaeda maritima (L.) Dumort)에 대하여 최소 복원 및 보존 면적의 크기를 AFLP (Amplified Fragment Length Polymorphism)기법을 이용한 유전적 다양성 정보에 근거하여 판정하였다. 각 종들의 조사 지역 개체군내에서 유전적 다양성 지수 $\Psi_{ST}$값은 갈대(P. communis)는 0.3856, 칠면초(S. japonica)는 0.1445, 해홍나물(S. maritima)은 0.1669 그리고 갯잔디(Z. sinica) 0.2422을 나타내었다. 칠면초가 0.1445로 가장 낮은 값을 나타내었고 갈대가 0.3856로 가장 높은 값을 나타내었다. 즉, 본 조사 대상 군락 중에서 칠면초 개체군이 가장 높은 유전적 다양성을 나타내었고, 갈대 개체군이 가장 낮은 유전적 다양성을 나타내는 것으로 판단되었다. 한편, 각 개체군별 단위 면적당 유전적 다양성 지수에 근거한 최소 복원 및 보존 면적은 갈대(P. communis)는 $500{\times}500m^2$, 칠면초(S. japonica), 해홍나물(S. maritima), 그리고 갯잔디(Z. sinica)들은 각각 $100\times100m^2$로 판정되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.912-919
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• 2017

Objective: Identification of the candidate genes that play key roles in phenotypic variations can provide new information about evolution and positive selection. Interleukin (IL)-32 is involved in many biological processes, however, its role for the immune response against various diseases in mammals is poorly understood. Therefore, the current investigation was performed for the better understanding of the molecular evolution and the positive selection of single nucleotide polymorphisms in IL-32 gene. Methods: By using fixation index ($F_{ST}$) based method, IL-32 (9375) gene was found to be outlier and under significant positive selection with the provisional combined allocation of mean heterozygosity and $F_{ST}$. Using nucleotide sequences of 11 mammalian species from National Center for Biotechnology Information database, the evolutionary selection of IL-32 gene was determined using Maximum likelihood model method, through four models (M1a, M2a, M7, and M8) in Codeml program of phylogenetic analysis by maximum liklihood. Results: IL-32 is detected under positive selection using the $F_{ST}$ simulations method. The phylogenetic tree revealed that goat IL-32 was in close resemblance with sheep IL-32. The coding nucleotide sequences were compared among 11 species and it was found that the goat IL-32 gene shared identity with sheep (96.54%), bison (91.97%), camel (58.39%), cat (56.59%), buffalo (56.50%), human (56.13%), dog (50.97%), horse (54.04%), and rabbit (53.41%) respectively. Conclusion: This study provides evidence for IL-32 gene as under significant positive selection in goat.